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The map label for this gene is trpE [H]

Identifier: 145297019

GI number: 145297019

Start: 3236376

End: 3237932

Strand: Direct

Name: trpE [H]

Synonym: cgR_2916

Alternate gene names: 145297019

Gene position: 3236376-3237932 (Clockwise)

Preceding gene: 145297017

Following gene: 145297020

Centisome position: 97.65

GC content: 57.1

Gene sequence:

>1557_bases
ATGAGCACGAATCCCCATGTTTTCTCCCTAGATGTCCGCTATCACGAGGATGCTTCTGCGTTGTTTGCCCACTTGGGTGG
CACGACGGCCGATGATGCAGCTCTTTTAGAAAGCGCCGATATCACCACCAAGAATGGTATTTCTTCCCTCGCGGTGTTGA
AGAGTTCGGTGCGCATTACGTGCACGGGCAACACGGTGGTAACGCAGCCACTGACGGACTCGGGTAGGACAGTGGTCGCA
CGCCTGACACAGCAGCTTTCCCAGTACAACACCGCAGAGAATACTTTTAGCTTCCCGGCCTCCGATGCTGTTGATGAGCG
CGAGCGCCTCACCGCACCAAGCACCATCGAAGTGCTGCGCAAGTTGCAGTTCGAGTCCGGCTACAGTGACGCGTCCCTGC
CACTGCTCATGGGCGGTTTCGCCTTTGATTTCTTAGAAACCTTTGAAACGCTCCCCGCGGTCGAGGAGAGCGTCAACACT
TACCCCGATTACCAGTTCGTCCTCGCGGAAATCGTCCTGGACATCAATCACCAGAACCAGACCGCCAAACTCACCGGCGT
CTCCAACACCCCAGGCGAGCTCGAGGCCGAGCTCAACAAGCTTTCATTGCTTATCGACGCCGCCCTCCCCGCAACTGAAC
ACGCCTACCAAACCACCCCTCACGACGGCGACACTCTTCGCGTTGTGGCTGATATTCCCGATGCTCAGTTCCGCACCCAG
ATCAATGAGCTGAAAGAAAACATTTACAACGGTGACATCTACCAAGTTGTCCCGGCGCGCACTTTCACCGCACCATGTCC
TGATGCATTCGCTGCTTATCTGCAGCTGCGTGCCACCAACCCGTCGCCGTACATGTTCTATATCCGTGGCCTCAACGAAG
GCCGCTCCTATGAACTTTTTGGCGCATCCCCTGAGTCCAACCTCAAGTTCACCGCTGCTAACCGTGAGCTGCAGCTGTAC
CCAATCGCAGGTACCCGCCCCCGTGGACTCAACCCAGATGGCTCCATCAACGATGAGCTAGATATCCGCAATGAGTTGGA
TATGCGCACTGATGCCAAAGAGATCGCGGAGCACACCATGCTTGTCGATCTCGCCCGCAACGACCTGGCCCGCGTCTCGG
TCCCAGCGTCGCGCCGGGTTGCGGATCTTTTGCAGGTGGATCGCTATTCCCGCGTGATGCACTTGGTGTCCCGTGTGACG
GCGACGTTGGACCCAGAGCTTGATGCTTTGGACGCCTATCGGGCGTGCATGAATATGGGCACGTTGACCGGCGCTCCGAA
GTTGCGCGCTATGGAGCTGTTGCGCGGCGTCGAAAAGCGCAGGCGTGGTTCTTATGGTGGGGCAGTGGGGTACCTGCGCG
GCAATGGCGATATGGATAATTGCATTGTTATTCGTTCGGCGTTTGTCCAGGATGGTGTGGCTGCTGTGCAGGCTGGTGCG
GGTGTGGTCCGCGATTCTAATCCTCAATCTGAAGCCGATGAGACGTTGCACAAGGCGTATGCCGTGTTGAATGCCATTGC
GCTTGCTGCTGGTTCCACTTTGGAGGTCATCCGATGA

Upstream 100 bases:

>100_bases
AACCGGCAAGCCCTGGATCGAATGAAGCTCGCAGCGAGTAATTATTTGATGTTTCCCAGAAAGGCTTCAGCCCCACAATG
ATTTCCTCGGTAGGTGCCCC

Downstream 100 bases:

>100_bases
CACACGTTGTTCTCATTGATAATCACGATTCTTTTGTCTACAACCTGGTGGATGCGTTCGCCGTGGCCGGTTATAAGTGC
ACGGTGTTCCGCAATACGGT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSTNPHVFSLDVRYHEDASALFAHLGGTTADDAALLESADITTKNGISSLAVLKSSVRITCTGNTVVTQPLTDSGRTVVA
RLTQQLSQYNTAENTFSFPASDAVDERERLTAPSTIEVLRKLQFESGYSDASLPLLMGGFAFDFLETFETLPAVEESVNT
YPDYQFVLAEIVLDINHQNQTAKLTGVSNTPGELEAELNKLSLLIDAALPATEHAYQTTPHDGDTLRVVADIPDAQFRTQ
INELKENIYNGDIYQVVPARTFTAPCPDAFAAYLQLRATNPSPYMFYIRGLNEGRSYELFGASPESNLKFTAANRELQLY
PIAGTRPRGLNPDGSINDELDIRNELDMRTDAKEIAEHTMLVDLARNDLARVSVPASRRVADLLQVDRYSRVMHLVSRVT
ATLDPELDALDAYRACMNMGTLTGAPKLRAMELLRGVEKRRRGSYGGAVGYLRGNGDMDNCIVIRSAFVQDGVAAVQAGA
GVVRDSNPQSEADETLHKAYAVLNAIALAAGSTLEVIR

Sequences:

>Translated_518_residues
MSTNPHVFSLDVRYHEDASALFAHLGGTTADDAALLESADITTKNGISSLAVLKSSVRITCTGNTVVTQPLTDSGRTVVA
RLTQQLSQYNTAENTFSFPASDAVDERERLTAPSTIEVLRKLQFESGYSDASLPLLMGGFAFDFLETFETLPAVEESVNT
YPDYQFVLAEIVLDINHQNQTAKLTGVSNTPGELEAELNKLSLLIDAALPATEHAYQTTPHDGDTLRVVADIPDAQFRTQ
INELKENIYNGDIYQVVPARTFTAPCPDAFAAYLQLRATNPSPYMFYIRGLNEGRSYELFGASPESNLKFTAANRELQLY
PIAGTRPRGLNPDGSINDELDIRNELDMRTDAKEIAEHTMLVDLARNDLARVSVPASRRVADLLQVDRYSRVMHLVSRVT
ATLDPELDALDAYRACMNMGTLTGAPKLRAMELLRGVEKRRRGSYGGAVGYLRGNGDMDNCIVIRSAFVQDGVAAVQAGA
GVVRDSNPQSEADETLHKAYAVLNAIALAAGSTLEVIR
>Mature_517_residues
STNPHVFSLDVRYHEDASALFAHLGGTTADDAALLESADITTKNGISSLAVLKSSVRITCTGNTVVTQPLTDSGRTVVAR
LTQQLSQYNTAENTFSFPASDAVDERERLTAPSTIEVLRKLQFESGYSDASLPLLMGGFAFDFLETFETLPAVEESVNTY
PDYQFVLAEIVLDINHQNQTAKLTGVSNTPGELEAELNKLSLLIDAALPATEHAYQTTPHDGDTLRVVADIPDAQFRTQI
NELKENIYNGDIYQVVPARTFTAPCPDAFAAYLQLRATNPSPYMFYIRGLNEGRSYELFGASPESNLKFTAANRELQLYP
IAGTRPRGLNPDGSINDELDIRNELDMRTDAKEIAEHTMLVDLARNDLARVSVPASRRVADLLQVDRYSRVMHLVSRVTA
TLDPELDALDAYRACMNMGTLTGAPKLRAMELLRGVEKRRRGSYGGAVGYLRGNGDMDNCIVIRSAFVQDGVAAVQAGAG
VVRDSNPQSEADETLHKAYAVLNAIALAAGSTLEVIR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=509, Percent_Identity=47.9371316306483, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788114, Length=373, Percent_Identity=32.4396782841823, Blast_Score=181, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6320935, Length=277, Percent_Identity=42.2382671480144, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324361, Length=347, Percent_Identity=26.2247838616715, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 56504; Mature: 56373

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNPHVFSLDVRYHEDASALFAHLGGTTADDAALLESADITTKNGISSLAVLKSSVRIT
CCCCCEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCEEEE
CTGNTVVTQPLTDSGRTVVARLTQQLSQYNTAENTFSFPASDAVDERERLTAPSTIEVLR
ECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
KLQFESGYSDASLPLLMGGFAFDFLETFETLPAVEESVNTYPDYQFVLAEIVLDINHQNQ
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
TAKLTGVSNTPGELEAELNKLSLLIDAALPATEHAYQTTPHDGDTLRVVADIPDAQFRTQ
EEEEEECCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
INELKENIYNGDIYQVVPARTFTAPCPDAFAAYLQLRATNPSPYMFYIRGLNEGRSYELF
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEE
GASPESNLKFTAANRELQLYPIAGTRPRGLNPDGSINDELDIRNELDMRTDAKEIAEHTM
CCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHH
LVDLARNDLARVSVPASRRVADLLQVDRYSRVMHLVSRVTATLDPELDALDAYRACMNMG
HHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TLTGAPKLRAMELLRGVEKRRRGSYGGAVGYLRGNGDMDNCIVIRSAFVQDGVAAVQAGA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCHHHHHCCC
GVVRDSNPQSEADETLHKAYAVLNAIALAAGSTLEVIR
CCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
STNPHVFSLDVRYHEDASALFAHLGGTTADDAALLESADITTKNGISSLAVLKSSVRIT
CCCCEEEEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCEEEE
CTGNTVVTQPLTDSGRTVVARLTQQLSQYNTAENTFSFPASDAVDERERLTAPSTIEVLR
ECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHH
KLQFESGYSDASLPLLMGGFAFDFLETFETLPAVEESVNTYPDYQFVLAEIVLDINHQNQ
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
TAKLTGVSNTPGELEAELNKLSLLIDAALPATEHAYQTTPHDGDTLRVVADIPDAQFRTQ
EEEEEECCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
INELKENIYNGDIYQVVPARTFTAPCPDAFAAYLQLRATNPSPYMFYIRGLNEGRSYELF
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHEEEEECCCCCEEEEEEECCCCCCEEEE
GASPESNLKFTAANRELQLYPIAGTRPRGLNPDGSINDELDIRNELDMRTDAKEIAEHTM
CCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHH
LVDLARNDLARVSVPASRRVADLLQVDRYSRVMHLVSRVTATLDPELDALDAYRACMNMG
HHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TLTGAPKLRAMELLRGVEKRRRGSYGGAVGYLRGNGDMDNCIVIRSAFVQDGVAAVQAGA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCHHHHHCCC
GVVRDSNPQSEADETLHKAYAVLNAIALAAGSTLEVIR
CCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3808947; 2263476; 12948626; 3609747; 3667535 [H]