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The map label for this gene is pckG [H]

Identifier: 145296851

GI number: 145296851

Start: 3045858

End: 3047690

Strand: Reverse

Name: pckG [H]

Synonym: cgR_2751

Alternate gene names: 145296851

Gene position: 3047690-3045858 (Counterclockwise)

Preceding gene: 145296858

Following gene: 145296848

Centisome position: 91.96

GC content: 57.5

Gene sequence:

>1833_bases
ATGACTACTGCTGCAATCAGGGGCCTTCAGGGTGAGGCGCCGACCAAGAATAAGGAACTGCTGAACTGGATCGCAGACGC
CGTCGAGCTCTTCCAGCCTGAGGCTGTTGTGTTCGTTGATGGATCCCAGGCTGAGTGGGATCGCATGGCGGAGGATCTTG
TTGAAGCCGGTACCCTCATCAAGCTCAACGAGGAAAAGCGTCCGAACAGCTACCTAGCTCGTTCCAACCCATCTGACGTT
GCGCGCGTTGAGTCCCGCACCTTCATCTGCTCCGAGAAGGAAGAAGATGCTGGCCCAACCAACAACTGGGCTCCACCACA
GGCAATGAAGGACGAAATGTCCAAGCATTACGCTGGTTCCATGAAGGGGCGCACCATGTACGTCGTGCCTTTCTGCATGG
GTCCAATCAGCGATCCGGACCCTAAGCTTGGTGTGCAGCTCACTGACTCCGAGTACGTTGTCATGTCCATGCGCATCATG
ACCCGCATGGGTATTGAAGCGCTGGACAAGATCGGCGCGAACGGTAGCTTCGTTAAGTGCCTCCACTCCGTTGGTGCTCC
TTTGGAGCCAGGCCAGGAAGACGTTGCATGGCCTTGCAACGACACCAAGTACATCACCCAGTTCCCAGAGACCAAGGAAA
TTTGGTCCTACGGTTCCGGCTACGGCGGAAACGCAATTCTGGCAAAGAAGTGCTACGCACTGCGTATCGCATCTGTCATG
GCTCGCGAAGAAGGCTGGATGGCTGAGCACATGCTCATCCTGAAGCTGATCAACCCAGAGGGCAAGGCGTACCACATCGC
AGCAGCATTCCCATCTGCTTGTGGCAAGACCAACCTCGCCATGATCACTCCAACCATCCCAGGCTGGACCGCTCAGGTTG
TTGGCGACGACATTGCTTGGCTGAAGCTGCGCGAGGACGGCCTCTACGCAGTTAACCCAGAAAACGGTTTCTTCGGTGTT
GCTCCAGGCACCAACTACGCATCTAACCCAATCGCGATGAAGACCATGGAACCAGGCAACACCCTGTTCACCAACGTGGC
ACTCACCGACGACGGCGACATCTGGTGGGAGGGCATGGATGGCGACGCCCCCGCTCACCTCATTGACTGGAAGGGTAACG
ACTGGACCCCAGAGTCCGACGAAAACGCTGCTCACCCTAACTCCCGTTACTGCGTAGCAATCGACCAGTCCCCAGCAGCA
GCACCTGAGTTCAACGACTGGGAAGGCGTCAAGATCGACGCAATCCTCTTCGGTGGACGTCGCGCAGACACCGTCCCACT
GGTTACTCAGACCTACGACTGGGAGCACGGCACCATGGTTGGTGCACTGCTCGCATCCGGTCAGACCGCAGCTTCCGCAG
AAGCAAAGGTCGGCACACTCCGCCACGACCCAATGGCAATGCTCCCATTCATTGGCTACAACGCTGGTGAATACCTGCAG
AACTGGATTGACATGGGCAACAAGGGTGGCGACAAGATGCCATCCATCTTCCTGGTCAACTGGTTCCGCCGTGGCGAAGA
TGGACGCTTCCTGTGGCCTGGCTTCGGCGACAACTCCCGCGTTCTGAAGTGGGTCATCGACCGCATCGAAGGCCGCGTTG
GCGCAGACGAGACCGTTGTTGGACACACCGCTAAGGCTGAAGACCTCGACCTCGACGGCCTCGACACCCCAATTGAGGAT
GTCAAGGAAGCACTGACCGCTCCTGCAGAGCAGTGGGCGAACGACGTTCAAGACAACGCCGAGTACCTCACTTTCCTCGG
ACCACGTGTTCCTGCAGAGGTTCACAGCCAGTTCGATGCTCTGAAGGCCCGCATTTCAGCAGCTCACGCTTAA

Upstream 100 bases:

>100_bases
ATGTGTCCGTTGTCTCACCTAAAGTTTTAACTAGTTCTGTATCTGAAAGCTACGCTAGGGGGCGAGAACTCTGTCGAATG
ACACAAAATCTGGAGAAGTA

Downstream 100 bases:

>100_bases
AGTTCACGCTTAAGAACTGCTAAATAACAAGAAAGGCTCCCGAAAGGGAGCCTTTCTTGTCGTTAAGCGATGAATTCCTC
AAAACCTCAGTGCTTTTTAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTTAAIRGLQGEAPTKNKELLNWIADAVELFQPEAVVFVDGSQAEWDRMAEDLVEAGTLIKLNEEKRPNSYLARSNPSDV
ARVESRTFICSEKEEDAGPTNNWAPPQAMKDEMSKHYAGSMKGRTMYVVPFCMGPISDPDPKLGVQLTDSEYVVMSMRIM
TRMGIEALDKIGANGSFVKCLHSVGAPLEPGQEDVAWPCNDTKYITQFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
AREEGWMAEHMLILKLINPEGKAYHIAAAFPSACGKTNLAMITPTIPGWTAQVVGDDIAWLKLREDGLYAVNPENGFFGV
APGTNYASNPIAMKTMEPGNTLFTNVALTDDGDIWWEGMDGDAPAHLIDWKGNDWTPESDENAAHPNSRYCVAIDQSPAA
APEFNDWEGVKIDAILFGGRRADTVPLVTQTYDWEHGTMVGALLASGQTAASAEAKVGTLRHDPMAMLPFIGYNAGEYLQ
NWIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGDNSRVLKWVIDRIEGRVGADETVVGHTAKAEDLDLDGLDTPIED
VKEALTAPAEQWANDVQDNAEYLTFLGPRVPAEVHSQFDALKARISAAHA

Sequences:

>Translated_610_residues
MTTAAIRGLQGEAPTKNKELLNWIADAVELFQPEAVVFVDGSQAEWDRMAEDLVEAGTLIKLNEEKRPNSYLARSNPSDV
ARVESRTFICSEKEEDAGPTNNWAPPQAMKDEMSKHYAGSMKGRTMYVVPFCMGPISDPDPKLGVQLTDSEYVVMSMRIM
TRMGIEALDKIGANGSFVKCLHSVGAPLEPGQEDVAWPCNDTKYITQFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
AREEGWMAEHMLILKLINPEGKAYHIAAAFPSACGKTNLAMITPTIPGWTAQVVGDDIAWLKLREDGLYAVNPENGFFGV
APGTNYASNPIAMKTMEPGNTLFTNVALTDDGDIWWEGMDGDAPAHLIDWKGNDWTPESDENAAHPNSRYCVAIDQSPAA
APEFNDWEGVKIDAILFGGRRADTVPLVTQTYDWEHGTMVGALLASGQTAASAEAKVGTLRHDPMAMLPFIGYNAGEYLQ
NWIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGDNSRVLKWVIDRIEGRVGADETVVGHTAKAEDLDLDGLDTPIED
VKEALTAPAEQWANDVQDNAEYLTFLGPRVPAEVHSQFDALKARISAAHA
>Mature_609_residues
TTAAIRGLQGEAPTKNKELLNWIADAVELFQPEAVVFVDGSQAEWDRMAEDLVEAGTLIKLNEEKRPNSYLARSNPSDVA
RVESRTFICSEKEEDAGPTNNWAPPQAMKDEMSKHYAGSMKGRTMYVVPFCMGPISDPDPKLGVQLTDSEYVVMSMRIMT
RMGIEALDKIGANGSFVKCLHSVGAPLEPGQEDVAWPCNDTKYITQFPETKEIWSYGSGYGGNAILAKKCYALRIASVMA
REEGWMAEHMLILKLINPEGKAYHIAAAFPSACGKTNLAMITPTIPGWTAQVVGDDIAWLKLREDGLYAVNPENGFFGVA
PGTNYASNPIAMKTMEPGNTLFTNVALTDDGDIWWEGMDGDAPAHLIDWKGNDWTPESDENAAHPNSRYCVAIDQSPAAA
PEFNDWEGVKIDAILFGGRRADTVPLVTQTYDWEHGTMVGALLASGQTAASAEAKVGTLRHDPMAMLPFIGYNAGEYLQN
WIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGDNSRVLKWVIDRIEGRVGADETVVGHTAKAEDLDLDGLDTPIEDV
KEALTAPAEQWANDVQDNAEYLTFLGPRVPAEVHSQFDALKARISAAHA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=619, Percent_Identity=48.1421647819063, Blast_Score=565, Evalue=1e-161,
Organism=Homo sapiens, GI187281517, Length=613, Percent_Identity=46.8189233278956, Blast_Score=557, Evalue=1e-158,
Organism=Homo sapiens, GI66346723, Length=411, Percent_Identity=47.6885644768856, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17555492, Length=626, Percent_Identity=46.3258785942492, Blast_Score=549, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71989645, Length=617, Percent_Identity=45.8670988654781, Blast_Score=539, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71989658, Length=617, Percent_Identity=45.8670988654781, Blast_Score=538, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71989653, Length=595, Percent_Identity=46.5546218487395, Blast_Score=529, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI32564411, Length=556, Percent_Identity=48.5611510791367, Blast_Score=520, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI32564407, Length=524, Percent_Identity=49.4274809160305, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71989649, Length=516, Percent_Identity=46.7054263565892, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI32564409, Length=370, Percent_Identity=47.8378378378378, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17540956, Length=589, Percent_Identity=23.2597623089983, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24655085, Length=597, Percent_Identity=48.2412060301507, Blast_Score=570, Evalue=1e-162,
Organism=Drosophila melanogaster, GI24655082, Length=611, Percent_Identity=47.6268412438625, Blast_Score=560, Evalue=1e-159,
Organism=Drosophila melanogaster, GI161077226, Length=569, Percent_Identity=49.0333919156415, Blast_Score=540, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24655088, Length=502, Percent_Identity=49.402390438247, Blast_Score=500, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 66862; Mature: 66731

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAIRGLQGEAPTKNKELLNWIADAVELFQPEAVVFVDGSQAEWDRMAEDLVEAGTLI
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEE
KLNEEKRPNSYLARSNPSDVARVESRTFICSEKEEDAGPTNNWAPPQAMKDEMSKHYAGS
EECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
MKGRTMYVVPFCMGPISDPDPKLGVQLTDSEYVVMSMRIMTRMGIEALDKIGANGSFVKC
CCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHH
LHSVGAPLEPGQEDVAWPCNDTKYITQFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AREEGWMAEHMLILKLINPEGKAYHIAAAFPSACGKTNLAMITPTIPGWTAQVVGDDIAW
HHHCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEE
LKLREDGLYAVNPENGFFGVAPGTNYASNPIAMKTMEPGNTLFTNVALTDDGDIWWEGMD
EEEECCCEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCC
GDAPAHLIDWKGNDWTPESDENAAHPNSRYCVAIDQSPAAAPEFNDWEGVKIDAILFGGR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCC
RADTVPLVTQTYDWEHGTMVGALLASGQTAASAEAKVGTLRHDPMAMLPFIGYNAGEYLQ
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHCCCCHHHHHH
NWIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGDNSRVLKWVIDRIEGRVGADETVV
HHHHCCCCCCCCCCEEEEEEHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEE
GHTAKAEDLDLDGLDTPIEDVKEALTAPAEQWANDVQDNAEYLTFLGPRVPAEVHSQFDA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
LKARISAAHA
HHHHHHHCCC
>Mature Secondary Structure 
TTAAIRGLQGEAPTKNKELLNWIADAVELFQPEAVVFVDGSQAEWDRMAEDLVEAGTLI
CCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEE
KLNEEKRPNSYLARSNPSDVARVESRTFICSEKEEDAGPTNNWAPPQAMKDEMSKHYAGS
EECCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
MKGRTMYVVPFCMGPISDPDPKLGVQLTDSEYVVMSMRIMTRMGIEALDKIGANGSFVKC
CCCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHH
LHSVGAPLEPGQEDVAWPCNDTKYITQFPETKEIWSYGSGYGGNAILAKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AREEGWMAEHMLILKLINPEGKAYHIAAAFPSACGKTNLAMITPTIPGWTAQVVGDDIAW
HHHCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEE
LKLREDGLYAVNPENGFFGVAPGTNYASNPIAMKTMEPGNTLFTNVALTDDGDIWWEGMD
EEEECCCEEEECCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCEEEECCC
GDAPAHLIDWKGNDWTPESDENAAHPNSRYCVAIDQSPAAAPEFNDWEGVKIDAILFGGR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCC
RADTVPLVTQTYDWEHGTMVGALLASGQTAASAEAKVGTLRHDPMAMLPFIGYNAGEYLQ
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHCCCCHHHHHH
NWIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGDNSRVLKWVIDRIEGRVGADETVV
HHHHCCCCCCCCCCEEEEEEHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEE
GHTAKAEDLDLDGLDTPIEDVKEALTAPAEQWANDVQDNAEYLTFLGPRVPAEVHSQFDA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
LKARISAAHA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]