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The map label for this gene is rihA [H]

Identifier: 145296817

GI number: 145296817

Start: 3011658

End: 3012563

Strand: Reverse

Name: rihA [H]

Synonym: cgR_2720

Alternate gene names: 145296817

Gene position: 3012563-3011658 (Counterclockwise)

Preceding gene: 145296821

Following gene: 145296816

Centisome position: 90.9

GC content: 53.31

Gene sequence:

>906_bases
ATGATTCCTGTTCTCATCGACTGCGACACCGGCATCGACGACGCCCTCGCCCTGATCTACCTGGCTGCTTTGCATAAACG
TGGTGAAATCCAACTTTTTGGAGCAACGACCACCGCAGGAAATGTTGATGTGAAACAAACCGCCATCAATACCAGGTGGG
TGTTGGATCAGTGTGGATTAGCGGACATCCCGGTCCTCGCAGGACAACCTGAACCAAAGCACGTGCCGCTAGTGACTACT
CCAGAAACACACGGCGACCATGGCCTTGGTTATATAAACCCAGGTCACGTCGAAATTCCAGAAGGTGACTGGAAGCAGCT
GTGGAAAGAACACCTCAGTAACCCAGAAACTAAGCTGATTGTCACCGGGCCCGCCACCAACCTTGCGGAATTCGGGCCAG
TGGAAAACGTCACGCTGATGGGTGGCACCTACCTTTATCCAGGCAACACCACTCCAACGGCAGAATGGAATACCTGGGTT
GATCCACACGGAGCTAAAGAAGCATTCGCGGCAGCCCAAAAGCCCATCACGGTGTGTTCCTTGGGCGTGACCGAGCAGTT
TACTCTGAACCCGGACAGCCTTTCTACACTTATCAACACGCTTGGCAGCCAACCCATCGCAGAGCATTTACCTAAGATGC
TGCGCTTTTACTTTGAATTCCACGAAACGCAGGGCGAAGGCTACCTCGCTCAAATTCATGACTTGCTGACCTGCATGATT
GCCTTGGACAAGATCCCATTTTCAGGCCGTGAAGTAACCGTGGATGTGGAAGCCGATTCGACCTTGATGCGTGGCACCAC
TGTTGCAGATATTCGCGGACATTGGGGCAAGCCAGCTAACGCATTCCTTGTGGAAACCGCAGACATTGAGGCCGCCCACG
CGGAACTCCTCAGGGCAGTGGAATGA

Upstream 100 bases:

>100_bases
TTAGTCCTCTATGGCAGCAAGCCTGCCAGAGGAGCCTATCCAGCACTAGAACCCAACTAGAACCCAACCCCAAAACCAGA
AAACACTAAGCTCGTGAGAC

Downstream 100 bases:

>100_bases
AATAATCCGGTGCTGATGCAGGGCAACTCGCTTAATCTGGCAAACAACAGCGAGAGAAAGAAGCCCATGCCCACACCTGA
AGAACTTTTAGCAGCCCGCT

Product: hypothetical protein

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MIPVLIDCDTGIDDALALIYLAALHKRGEIQLFGATTTAGNVDVKQTAINTRWVLDQCGLADIPVLAGQPEPKHVPLVTT
PETHGDHGLGYINPGHVEIPEGDWKQLWKEHLSNPETKLIVTGPATNLAEFGPVENVTLMGGTYLYPGNTTPTAEWNTWV
DPHGAKEAFAAAQKPITVCSLGVTEQFTLNPDSLSTLINTLGSQPIAEHLPKMLRFYFEFHETQGEGYLAQIHDLLTCMI
ALDKIPFSGREVTVDVEADSTLMRGTTVADIRGHWGKPANAFLVETADIEAAHAELLRAVE

Sequences:

>Translated_301_residues
MIPVLIDCDTGIDDALALIYLAALHKRGEIQLFGATTTAGNVDVKQTAINTRWVLDQCGLADIPVLAGQPEPKHVPLVTT
PETHGDHGLGYINPGHVEIPEGDWKQLWKEHLSNPETKLIVTGPATNLAEFGPVENVTLMGGTYLYPGNTTPTAEWNTWV
DPHGAKEAFAAAQKPITVCSLGVTEQFTLNPDSLSTLINTLGSQPIAEHLPKMLRFYFEFHETQGEGYLAQIHDLLTCMI
ALDKIPFSGREVTVDVEADSTLMRGTTVADIRGHWGKPANAFLVETADIEAAHAELLRAVE
>Mature_301_residues
MIPVLIDCDTGIDDALALIYLAALHKRGEIQLFGATTTAGNVDVKQTAINTRWVLDQCGLADIPVLAGQPEPKHVPLVTT
PETHGDHGLGYINPGHVEIPEGDWKQLWKEHLSNPETKLIVTGPATNLAEFGPVENVTLMGGTYLYPGNTTPTAEWNTWV
DPHGAKEAFAAAQKPITVCSLGVTEQFTLNPDSLSTLINTLGSQPIAEHLPKMLRFYFEFHETQGEGYLAQIHDLLTCMI
ALDKIPFSGREVTVDVEADSTLMRGTTVADIRGHWGKPANAFLVETADIEAAHAELLRAVE

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=30.8441558441558, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786871, Length=300, Percent_Identity=28.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786213, Length=307, Percent_Identity=34.5276872964169, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320608, Length=266, Percent_Identity=27.0676691729323, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641837, Length=196, Percent_Identity=31.6326530612245, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 32703; Mature: 32703

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVLIDCDTGIDDALALIYLAALHKRGEIQLFGATTTAGNVDVKQTAINTRWVLDQCGL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHCCCEEEHHHCCC
ADIPVLAGQPEPKHVPLVTTPETHGDHGLGYINPGHVEIPEGDWKQLWKEHLSNPETKLI
CCCCEECCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCEEEE
VTGPATNLAEFGPVENVTLMGGTYLYPGNTTPTAEWNTWVDPHGAKEAFAAAQKPITVCS
EECCCCCHHHCCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEE
LGVTEQFTLNPDSLSTLINTLGSQPIAEHLPKMLRFYFEFHETQGEGYLAQIHDLLTCMI
CCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALDKIPFSGREVTVDVEADSTLMRGTTVADIRGHWGKPANAFLVETADIEAAHAELLRAV
HHHCCCCCCCEEEEEECCCCEEECCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHC
E
C
>Mature Secondary Structure
MIPVLIDCDTGIDDALALIYLAALHKRGEIQLFGATTTAGNVDVKQTAINTRWVLDQCGL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEHHHHCCCEEEHHHCCC
ADIPVLAGQPEPKHVPLVTTPETHGDHGLGYINPGHVEIPEGDWKQLWKEHLSNPETKLI
CCCCEECCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCEEEE
VTGPATNLAEFGPVENVTLMGGTYLYPGNTTPTAEWNTWVDPHGAKEAFAAAQKPITVCS
EECCCCCHHHCCCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEE
LGVTEQFTLNPDSLSTLINTLGSQPIAEHLPKMLRFYFEFHETQGEGYLAQIHDLLTCMI
CCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALDKIPFSGREVTVDVEADSTLMRGTTVADIRGHWGKPANAFLVETADIEAAHAELLRAV
HHHCCCCCCCEEEEEECCCCEEECCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA