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The map label for this gene is dapE [H]

Identifier: 145296811

GI number: 145296811

Start: 3004546

End: 3005871

Strand: Reverse

Name: dapE [H]

Synonym: cgR_2714

Alternate gene names: 145296811

Gene position: 3005871-3004546 (Counterclockwise)

Preceding gene: 145296813

Following gene: 145296809

Centisome position: 90.7

GC content: 54.3

Gene sequence:

>1326_bases
ATGACGCTTTACGACGAAACCCTCACCCTCCTTCAGGAACTTATCCGCAACGCCTGCGTGAATGATCTAACCCCAGATTC
AGGTCAGGAAATTAGAAACGCAGAGAGCCTGGAACGTTTCTTTGAAGGTACCCCGAATGTTGAGATCACCAAGCTGGAGC
CTCGTCCGGGTCGGACCTCTATTATCGTCACGGTTCCAGGCAGTGACCCTAATGCCGAGCCATTAACGCTGCTTGGTCAT
ACTGACGTTGTCCCTGTTGATCTGCCCAAATGGACTAAGGATCCCTTCGGTGCGGAGATCTCCGATGGACAAATTTGGGG
TCGCGGGTCTGTCGATATGCTCTTTATTACCGCAACTCAAGCGGCTGTCACCCGTCAAGTAGCTCGCGAAGGTGGACTGC
GCGGCACGCTGACATTCGTTGGCGTTGCCGATGAAGAAGCCCGCGGCGGACTCGGAGCGAAGTGGCTTTCCGAAGAACAC
CAAAACCTCTTCAGCTGGAAAAACTGCCTCTCCGAATCCGGTGGATCGCACCTTCCAGTCCACGACGGCAGCGACGCAGT
AGTAATTAACGTTGGAGAAAAGGGTGCAGCTCAACGTCGTATTCACGTCAATGGCGATGCCGGTCATGGTTCCATTCCCT
TTGACCGCGATAGCGCCATTGTCAAAATCGGTGAAGTCGCCCGCCGAATCGCTGCCGCCGATCTGAAGGTAGCCAAGGAT
GATATCTGGCAAGGCTTCGTCCAAGCGCACCGTTTCGACCCAGAAACGGAGCAGGCGCTGCTTAGCGGGACCTCCCCTGA
GGCCTATGCAGAGTTCGGCGGACTCTCCCGCTTCGCCCACGCGGTGTCTCATCTCACGATCGCCCAAACTGTGGTTCGTG
CAGGTCAAGCCATCAATGTCTTGCCGTCGCATGCGTACTTGGAACTGGATATCCGTACCCTTCCAGGCCAAACCAATGAC
TATGTTGATGACACCCTGCGTGGTGCTCTGGGCGATCTCGCCGATGAAGTAGAAATCGAACACCTCATCTCTGAAGAAGC
AACGGTGAGCCCAACTGATTCCAGGTTGTATAACACCTTGGAAAAAGTTCTTGGTGATTTCTTCCCCGATGCGCCTGTGG
TCCCAATTATTTCCTCTGGTGGCTCTGACCTGCGCTTTGGTCGTCGACTAGGCGGTGTTGGTTATGGTTTTGCAGTTCAT
GCACGTGAACGAACTTTGGCAGAAGCAATGGGGCAACTTCACTCCCATGACGAGGCGCTGTACCTGGAAGATCTTGAACT
GACTGTTCGGGGTTATGACTCCGTCGTGCGTGAATTCCTAGGCTAA

Upstream 100 bases:

>100_bases
ACGATACGGTCTAAAACTCCAGGGAAAACAGACAGAATCCAGCGTCAGTTAGCCGGAAATTCGATACAGTGGCGATGAAC
GCGATATTAGAAAGAAAAAG

Downstream 100 bases:

>100_bases
AAACATGAAGCAGGCGTCTTTTCAATTGAACGGGTTCTGAAACTAAGCACCGTTTGAAGCGCCACAACTGCAAGAACGAC
GCCTGCCAACAAGGGCATTC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTLYDETLTLLQELIRNACVNDLTPDSGQEIRNAESLERFFEGTPNVEITKLEPRPGRTSIIVTVPGSDPNAEPLTLLGH
TDVVPVDLPKWTKDPFGAEISDGQIWGRGSVDMLFITATQAAVTRQVAREGGLRGTLTFVGVADEEARGGLGAKWLSEEH
QNLFSWKNCLSESGGSHLPVHDGSDAVVINVGEKGAAQRRIHVNGDAGHGSIPFDRDSAIVKIGEVARRIAAADLKVAKD
DIWQGFVQAHRFDPETEQALLSGTSPEAYAEFGGLSRFAHAVSHLTIAQTVVRAGQAINVLPSHAYLELDIRTLPGQTND
YVDDTLRGALGDLADEVEIEHLISEEATVSPTDSRLYNTLEKVLGDFFPDAPVVPIISSGGSDLRFGRRLGGVGYGFAVH
ARERTLAEAMGQLHSHDEALYLEDLELTVRGYDSVVREFLG

Sequences:

>Translated_441_residues
MTLYDETLTLLQELIRNACVNDLTPDSGQEIRNAESLERFFEGTPNVEITKLEPRPGRTSIIVTVPGSDPNAEPLTLLGH
TDVVPVDLPKWTKDPFGAEISDGQIWGRGSVDMLFITATQAAVTRQVAREGGLRGTLTFVGVADEEARGGLGAKWLSEEH
QNLFSWKNCLSESGGSHLPVHDGSDAVVINVGEKGAAQRRIHVNGDAGHGSIPFDRDSAIVKIGEVARRIAAADLKVAKD
DIWQGFVQAHRFDPETEQALLSGTSPEAYAEFGGLSRFAHAVSHLTIAQTVVRAGQAINVLPSHAYLELDIRTLPGQTND
YVDDTLRGALGDLADEVEIEHLISEEATVSPTDSRLYNTLEKVLGDFFPDAPVVPIISSGGSDLRFGRRLGGVGYGFAVH
ARERTLAEAMGQLHSHDEALYLEDLELTVRGYDSVVREFLG
>Mature_440_residues
TLYDETLTLLQELIRNACVNDLTPDSGQEIRNAESLERFFEGTPNVEITKLEPRPGRTSIIVTVPGSDPNAEPLTLLGHT
DVVPVDLPKWTKDPFGAEISDGQIWGRGSVDMLFITATQAAVTRQVAREGGLRGTLTFVGVADEEARGGLGAKWLSEEHQ
NLFSWKNCLSESGGSHLPVHDGSDAVVINVGEKGAAQRRIHVNGDAGHGSIPFDRDSAIVKIGEVARRIAAADLKVAKDD
IWQGFVQAHRFDPETEQALLSGTSPEAYAEFGGLSRFAHAVSHLTIAQTVVRAGQAINVLPSHAYLELDIRTLPGQTNDY
VDDTLRGALGDLADEVEIEHLISEEATVSPTDSRLYNTLEKVLGDFFPDAPVVPIISSGGSDLRFGRRLGGVGYGFAVHA
RERTLAEAMGQLHSHDEALYLEDLELTVRGYDSVVREFLG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=392, Percent_Identity=25.2551020408163, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI4501901, Length=439, Percent_Identity=24.373576309795, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538640, Length=387, Percent_Identity=24.2894056847545, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71982710, Length=278, Percent_Identity=26.6187050359712, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71982082, Length=405, Percent_Identity=25.1851851851852, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24649210, Length=389, Percent_Identity=23.6503856041131, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47778; Mature: 47647

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYDETLTLLQELIRNACVNDLTPDSGQEIRNAESLERFFEGTPNVEITKLEPRPGRTS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHCCCCCEEEEEECCCCCCEE
IIVTVPGSDPNAEPLTLLGHTDVVPVDLPKWTKDPFGAEISDGQIWGRGSVDMLFITATQ
EEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEHH
AAVTRQVAREGGLRGTLTFVGVADEEARGGLGAKWLSEEHQNLFSWKNCLSESGGSHLPV
HHHHHHHHHHCCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HDGSDAVVINVGEKGAAQRRIHVNGDAGHGSIPFDRDSAIVKIGEVARRIAAADLKVAKD
CCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DIWQGFVQAHRFDPETEQALLSGTSPEAYAEFGGLSRFAHAVSHLTIAQTVVRAGQAINV
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
LPSHAYLELDIRTLPGQTNDYVDDTLRGALGDLADEVEIEHLISEEATVSPTDSRLYNTL
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EKVLGDFFPDAPVVPIISSGGSDLRFGRRLGGVGYGFAVHARERTLAEAMGQLHSHDEAL
HHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCEE
YLEDLELTVRGYDSVVREFLG
EEECEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
TLYDETLTLLQELIRNACVNDLTPDSGQEIRNAESLERFFEGTPNVEITKLEPRPGRTS
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHCCCCCEEEEEECCCCCCEE
IIVTVPGSDPNAEPLTLLGHTDVVPVDLPKWTKDPFGAEISDGQIWGRGSVDMLFITATQ
EEEEECCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEHH
AAVTRQVAREGGLRGTLTFVGVADEEARGGLGAKWLSEEHQNLFSWKNCLSESGGSHLPV
HHHHHHHHHHCCCEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HDGSDAVVINVGEKGAAQRRIHVNGDAGHGSIPFDRDSAIVKIGEVARRIAAADLKVAKD
CCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DIWQGFVQAHRFDPETEQALLSGTSPEAYAEFGGLSRFAHAVSHLTIAQTVVRAGQAINV
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
LPSHAYLELDIRTLPGQTNDYVDDTLRGALGDLADEVEIEHLISEEATVSPTDSRLYNTL
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EKVLGDFFPDAPVVPIISSGGSDLRFGRRLGGVGYGFAVHARERTLAEAMGQLHSHDEAL
HHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCEE
YLEDLELTVRGYDSVVREFLG
EEECEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]