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The map label for this gene is sir2 [H]

Identifier: 145296800

GI number: 145296800

Start: 2993672

End: 2995357

Strand: Reverse

Name: sir2 [H]

Synonym: cgR_2703

Alternate gene names: 145296800

Gene position: 2995357-2993672 (Counterclockwise)

Preceding gene: 145296803

Following gene: 145296799

Centisome position: 90.38

GC content: 54.92

Gene sequence:

>1686_bases
ATGACAACAACCACCGGAAGTGCCCGGCCAGCACGTGCCGCCAGGAAGCCTAAGCCCGAAGGCCAATGGAAAATCGACGG
CACCGAGCCGCTTAACCATGCCGAGGAAATTAAGCAAGAAGAACCCGCTTTTGCTGTCAAGCAGCGGGTCATTGATATTT
ACTCCAAGCAGGGTTTTTCTTCCATTGCACCGGATGACATTGCCCCACGCTTTAAGTGGTTGGGCATTTACACCCAGCGT
AAGCAGGATCTGGGCGGTGAACTGACCGGTCAGCTTCCTGATGATGAGCTGCAGGATGAGTACTTCATGATGCGTGTGCG
TTTTGATGGCGGACTGGCTTCCCCTGAGCGCCTGCGTGCCGTGGGTGAAATTTCTAGGGATTATGCTCGTTCCACCGCGG
ACTTCACCGACCGCCAGAACATTCAGCTGCACTGGATTCGTATTGAAGATGTGCCTGCGATCTGGGAGAAGCTAGAAACC
GTCGGACTGTCCACCATGCTTGGTTGCGGTGACGTTCCACGTGTTATCTTGGGCTCCCCAGTTTCTGGCGTAGCTGCTGA
AGAGCTGATCGATGCCACCCCGGCTATCGATGCGATTCGTGAGCGCTACCTAGACAAGGAAGAGTTCCACAACCTTCCTC
GTAAGTTTAAGACTGCTATCACTGGCAACCAGCGCCAGGATGTTACCCACGAAATCCAGGACGTTTCCTTCGTTCCTTCG
ATTCACCCAGAATTCGGCCCAGGATTTGAGTGCTTTGTGGGCGGTGGCCTGTCCACCAACCCAATGCTTGCTCAGCCACT
TGGTTCTTGGATTCCACTTGATGAGGTTCCAGAAGTGTGGGCTGGCGTCGCCGGCATTTTCCGCGACTACGGCTTCCGAC
GCCTGCGTAACCGTGCTCGCCTCAAGTTCTTGGTGGCACAGTGGGGTATTGAGAAGTTCCGTGAAGTTCTTGAGACCGAA
TACCTCGAGCGCAAGCTGATTGATGGCCCAGTTGTTACCACCAACCCTGGCTACCGTGACCACATTGGCATTCACCCACA
AAAGGACGGCAAGTTCTACCTCGGTGTGAAGCCAACCGTTGGACACACCACCGGTGAGCAGCTCATTGCCATTGCTGATG
TTGCAGAAAAGCACGGCATCACCAGGATTCGTACCACGGCGGAAAAGGAACTGCTCTTCCTCGATATTGAGCGAGAGAAC
CTTACTACCGTTGCACGCGACCTGGATGAAATCGGACTGTACTCTTCACCTTCCGAGTTCCGCCGCGGCATCATTTCCTG
CACCGGCTTGGAGTTCTGCAAGCTTGCGCACGCAACGACCAAGTCACGAGCAATTGAGCTTGTCGACGAACTGGAAGAGC
GACTCGGCGATTTGGAAGTTCCCATCAAGATCGCACTGAACGGTTGCCCTAACTCTTGCGCACGCACCCAGGTTTCCGAC
ATTGGATTCAAGGGACAGACCGTCACCGATGCTGACGGCAACCGCGTTGAAGGTTTCCAGGTTCACCTGGGCGGTTCCAT
GAACTTGGATCCAAACTTCGGACGCAAGCTCAAGGGCCACAAGGTTATTGCCGATGAAGTGGGAGAGTACGTCACTCGCG
TTGTTACCCACTTCAAGGAACAGCGCCACGAGGACGAGCACTTCCGCGATTGGGTCCAGCGGGCCGCTGAGGAAGATTTG
GTGTGA

Upstream 100 bases:

>100_bases
GAATGTAATGGTTGGTCTAGACAGAGCGGTACGTCTAAGTTTGCGGATAGATCAAACCGAGTGACATGTACTTCACTAGC
TCTTTAAGGATTAACTCCCC

Downstream 100 bases:

>100_bases
GTCTTCGGAGGAAACCCAATCCCAACCGCAACCACCCTCTGTACTGCCCATACTGCGCGGGAGAAGTTCTTTTCCCCGAT
GAGCAAACAGAATTCGCGTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTTTTGSARPARAARKPKPEGQWKIDGTEPLNHAEEIKQEEPAFAVKQRVIDIYSKQGFSSIAPDDIAPRFKWLGIYTQR
KQDLGGELTGQLPDDELQDEYFMMRVRFDGGLASPERLRAVGEISRDYARSTADFTDRQNIQLHWIRIEDVPAIWEKLET
VGLSTMLGCGDVPRVILGSPVSGVAAEELIDATPAIDAIRERYLDKEEFHNLPRKFKTAITGNQRQDVTHEIQDVSFVPS
IHPEFGPGFECFVGGGLSTNPMLAQPLGSWIPLDEVPEVWAGVAGIFRDYGFRRLRNRARLKFLVAQWGIEKFREVLETE
YLERKLIDGPVVTTNPGYRDHIGIHPQKDGKFYLGVKPTVGHTTGEQLIAIADVAEKHGITRIRTTAEKELLFLDIEREN
LTTVARDLDEIGLYSSPSEFRRGIISCTGLEFCKLAHATTKSRAIELVDELEERLGDLEVPIKIALNGCPNSCARTQVSD
IGFKGQTVTDADGNRVEGFQVHLGGSMNLDPNFGRKLKGHKVIADEVGEYVTRVVTHFKEQRHEDEHFRDWVQRAAEEDL
V

Sequences:

>Translated_561_residues
MTTTTGSARPARAARKPKPEGQWKIDGTEPLNHAEEIKQEEPAFAVKQRVIDIYSKQGFSSIAPDDIAPRFKWLGIYTQR
KQDLGGELTGQLPDDELQDEYFMMRVRFDGGLASPERLRAVGEISRDYARSTADFTDRQNIQLHWIRIEDVPAIWEKLET
VGLSTMLGCGDVPRVILGSPVSGVAAEELIDATPAIDAIRERYLDKEEFHNLPRKFKTAITGNQRQDVTHEIQDVSFVPS
IHPEFGPGFECFVGGGLSTNPMLAQPLGSWIPLDEVPEVWAGVAGIFRDYGFRRLRNRARLKFLVAQWGIEKFREVLETE
YLERKLIDGPVVTTNPGYRDHIGIHPQKDGKFYLGVKPTVGHTTGEQLIAIADVAEKHGITRIRTTAEKELLFLDIEREN
LTTVARDLDEIGLYSSPSEFRRGIISCTGLEFCKLAHATTKSRAIELVDELEERLGDLEVPIKIALNGCPNSCARTQVSD
IGFKGQTVTDADGNRVEGFQVHLGGSMNLDPNFGRKLKGHKVIADEVGEYVTRVVTHFKEQRHEDEHFRDWVQRAAEEDL
V
>Mature_560_residues
TTTTGSARPARAARKPKPEGQWKIDGTEPLNHAEEIKQEEPAFAVKQRVIDIYSKQGFSSIAPDDIAPRFKWLGIYTQRK
QDLGGELTGQLPDDELQDEYFMMRVRFDGGLASPERLRAVGEISRDYARSTADFTDRQNIQLHWIRIEDVPAIWEKLETV
GLSTMLGCGDVPRVILGSPVSGVAAEELIDATPAIDAIRERYLDKEEFHNLPRKFKTAITGNQRQDVTHEIQDVSFVPSI
HPEFGPGFECFVGGGLSTNPMLAQPLGSWIPLDEVPEVWAGVAGIFRDYGFRRLRNRARLKFLVAQWGIEKFREVLETEY
LERKLIDGPVVTTNPGYRDHIGIHPQKDGKFYLGVKPTVGHTTGEQLIAIADVAEKHGITRIRTTAEKELLFLDIERENL
TTVARDLDEIGLYSSPSEFRRGIISCTGLEFCKLAHATTKSRAIELVDELEERLGDLEVPIKIALNGCPNSCARTQVSDI
GFKGQTVTDADGNRVEGFQVHLGGSMNLDPNFGRKLKGHKVIADEVGEYVTRVVTHFKEQRHEDEHFRDWVQRAAEEDLV

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=528, Percent_Identity=24.0530303030303, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322597, Length=558, Percent_Identity=25.089605734767, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 63009; Mature: 62878

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTGSARPARAARKPKPEGQWKIDGTEPLNHAEEIKQEEPAFAVKQRVIDIYSKQGFS
CCCCCCCCCCHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SIAPDDIAPRFKWLGIYTQRKQDLGGELTGQLPDDELQDEYFMMRVRFDGGLASPERLRA
CCCCCHHCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCHHHHHH
VGEISRDYARSTADFTDRQNIQLHWIRIEDVPAIWEKLETVGLSTMLGCGDVPRVILGSP
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHCHHHHHCCCCCCHHHHCCC
VSGVAAEELIDATPAIDAIRERYLDKEEFHNLPRKFKTAITGNQRQDVTHEIQDVSFVPS
CCCHHHHHHHHCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IHPEFGPGFECFVGGGLSTNPMLAQPLGSWIPLDEVPEVWAGVAGIFRDYGFRRLRNRAR
CCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKFLVAQWGIEKFREVLETEYLERKLIDGPVVTTNPGYRDHIGIHPQKDGKFYLGVKPTV
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCC
GHTTGEQLIAIADVAEKHGITRIRTTAEKELLFLDIERENLTTVARDLDEIGLYSSPSEF
CCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHH
RRGIISCTGLEFCKLAHATTKSRAIELVDELEERLGDLEVPIKIALNGCPNSCARTQVSD
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
IGFKGQTVTDADGNRVEGFQVHLGGSMNLDPNFGRKLKGHKVIADEVGEYVTRVVTHFKE
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
QRHEDEHFRDWVQRAAEEDLV
HCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTTGSARPARAARKPKPEGQWKIDGTEPLNHAEEIKQEEPAFAVKQRVIDIYSKQGFS
CCCCCCCCCHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SIAPDDIAPRFKWLGIYTQRKQDLGGELTGQLPDDELQDEYFMMRVRFDGGLASPERLRA
CCCCCHHCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCHHHHHH
VGEISRDYARSTADFTDRQNIQLHWIRIEDVPAIWEKLETVGLSTMLGCGDVPRVILGSP
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHCHHHHHCCCCCCHHHHCCC
VSGVAAEELIDATPAIDAIRERYLDKEEFHNLPRKFKTAITGNQRQDVTHEIQDVSFVPS
CCCHHHHHHHHCCHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCC
IHPEFGPGFECFVGGGLSTNPMLAQPLGSWIPLDEVPEVWAGVAGIFRDYGFRRLRNRAR
CCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKFLVAQWGIEKFREVLETEYLERKLIDGPVVTTNPGYRDHIGIHPQKDGKFYLGVKPTV
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCC
GHTTGEQLIAIADVAEKHGITRIRTTAEKELLFLDIERENLTTVARDLDEIGLYSSPSEF
CCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHH
RRGIISCTGLEFCKLAHATTKSRAIELVDELEERLGDLEVPIKIALNGCPNSCARTQVSD
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
IGFKGQTVTDADGNRVEGFQVHLGGSMNLDPNFGRKLKGHKVIADEVGEYVTRVVTHFKE
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
QRHEDEHFRDWVQRAAEEDLV
HCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA