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The map label for this gene is purA [H]

Identifier: 145296759

GI number: 145296759

Start: 2948101

End: 2949390

Strand: Reverse

Name: purA [H]

Synonym: cgR_2663

Alternate gene names: 145296759

Gene position: 2949390-2948101 (Counterclockwise)

Preceding gene: 145296762

Following gene: 145296758

Centisome position: 88.99

GC content: 57.44

Gene sequence:

>1290_bases
ATGGCTGCAATCGTTATTGTCGGCGCTCAATGGGGCGATGAAGGCAAAGGTAAGGCCACGGATATTCTCGGCGGACTCGT
CGATTACGTGGTTAAGCCCAATGGCGGTAACAACGCTGGACACACTGTTGTGGTCGGCGGCGAGAAGTACGAGCTAAAGC
TCCTTCCTGCCGGCGTCCTCTCCGAAACGGCCACCCCAATTTTGGGCAACGGCGTTGTGATCAACCTTGAGGCACTGTTC
GAAGAAATCGACGGACTTGAGGCTCGCGGTGCGGATGCATCCCGCCTGCGCATCTCTGCAAACGCTCACCTGGTTGCTCC
ATACCACCAGGTGATGGACCGTGTTCAGGAACGCTTCCTGGGCAAGCGCGCTATCGGCACCACCGGCCGTGGCATCGGCC
CAACTTACGCGGACAAGGTGTCCCGCGTGGGAATCCGTGTTCAAGACATTTTCGACGAATCCATCCTTCGCCAAAAAGTC
GAGTCTGCCCTGGATTACAAGAACCAGGTCCTGGTGAAGATGTACAACCGCAAGGCCATCGTCGCTGAGGAAATCGTGCA
GTACTTCCTCTCCTACGCTGATCGTCTGCGCCCCATGGTCATCGATGCCACCTTGGTGCTCAACGAGGCACTTGATCAGG
GCAAGCACGTTCTCATGGAAGGTGGCCAGGCAACCATGCTCGACGTGGACCACGGCACCTACCCATTCGTCACCTCCTCC
AACCCAACCGCCGGTGGCGCAAGCGTTGGTTCAGGTATCGGCCCAACCAAGATCACCAGCTCCTTGGGCATCATCAAGGC
CTACACCACTCGTGTTGGTGCCGGCCCATTCCCAACTGAGCTGTTTGATAAGTGGGGCGAGTACCTGCAGACCGTCGGTG
GCGAGGTCGGCGTGAACACCGGCCGTAAGCGTCGCTGTGGCTGGTACGACTCCGTGATTGCTCGTTACGCATCCCGCGTC
AACGGCTTCACCGACTACTTCCTCACCAAGCTGGACGTGCTCACCGGTATCGGTGAAATCCCAATCTGCGTAGCTTACGA
CGTTGATGGTGTTCGCCACGATGAAATGCCACTGACCCAGTCAGAGTTCCACCACGCAACCCCAATCTTTGAAACCATGC
CTGCATGGGACGAAGACATCACCGACTGCAAGACCTTCGAGGATCTTCCACAAAAGGCCCAGGACTACGTCCGACGCCTG
GAAGAACTCTCTGGTGCTCGCTTCTCCTACATCGGTGTTGGACCTGGTCGCGATCAGACCATCGTCCTGCATGACGTTTT
GGAAGGCTAA

Upstream 100 bases:

>100_bases
GATGATTAAAAACCTCACATGGGATAGTAAGATGAAATAACGTCTGTGTGCGCACACGCGTGCATACTCCAACTTCATGG
ATCGGATGTGACGTAAACCA

Downstream 100 bases:

>100_bases
AACACTTTGGTTTACGCCAGTTAATGAGAAAAATCCCTGCAACAGTTTGATGTTGCGGGGATTTTTCTGTCTGTAGGTTG
CCCCCAAATTTAAGGCATTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALF
EEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKV
ESALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRL
EELSGARFSYIGVGPGRDQTIVLHDVLEG

Sequences:

>Translated_429_residues
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALF
EEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKV
ESALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRL
EELSGARFSYIGVGPGRDQTIVLHDVLEG
>Mature_428_residues
AAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALFE
EIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVE
SALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSSN
PTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRVN
GFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLE
ELSGARFSYIGVGPGRDQTIVLHDVLEG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=46.0280373831776, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=44.6511627906977, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=45.7286432160804, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=47.6415094339623, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.3611111111111, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.3611111111111, Blast_Score=327, Evalue=8e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=43.0555555555556, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=44.5754716981132, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46656; Mature: 46524

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
CEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SETATPILGNGVVINLEALFEEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFL
HHCCCCEECCCEEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVESALDYKNQVLVKMYNRKAI
CCHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNT
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
SEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLEELSGARFSYIGVGPGRDQT
HHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCE
IVLHDVLEG
EEEEEHHCC
>Mature Secondary Structure 
AAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
EEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SETATPILGNGVVINLEALFEEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFL
HHCCCCEECCCEEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVESALDYKNQVLVKMYNRKAI
CCHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNT
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
SEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLEELSGARFSYIGVGPGRDQT
HHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCE
IVLHDVLEG
EEEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]