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The map label for this gene is purT

Identifier: 145296758

GI number: 145296758

Start: 2946700

End: 2947926

Strand: Reverse

Name: purT

Synonym: cgR_2662

Alternate gene names: 145296758

Gene position: 2947926-2946700 (Counterclockwise)

Preceding gene: 145296759

Following gene: 145296757

Centisome position: 88.95

GC content: 55.5

Gene sequence:

>1227_bases
ATGTACATCCCAGAGTCGATCGGCACCCCTTTGACCCCCAATGCCACGAAAGTGATGCTGCTGGGATCAGGAGAATTAGG
CAAAGAAGTAGCCATTGCTTTCCAGCGTCTCGGCCTGGAAGTCCATGCTGTTGATCGCTACGAACATGCCCCAGCCCACC
AGGTCGCTCACTTCTCCTATGTCATCGACATGACAGATGCAGCCCAGGTGCGGGAATTGGTGGAGCGTGTGCGCCCAGAT
TTTGTCATTCCTGAAATCGAAGCACTGGCAACCGATGAACTGGTGAAGATCGAAGAAGAGGGGCTAGCTACCATCGTGCC
CACTGCACGTGCAGCCAAGCTGACCATGAACCGCGAAGGCATCCGCAAGCTGGCAGCAGAGGAACTGGGTCTTCCAACCT
CCAACTATGAGTTCTGCTCCACTTTCGAGGAATTCTCCGCAGCTGCTGAAAAGCTTGGTTACCCGAACGTGGTGAAACCA
GTGATGAGTTCTTCCGGCAAGGGCCAATCTGTTTTGCGTAGTTCAGACGATCTGCAGGCAGCATGGGATTATGCGATGAG
CGGTGCACGCGTGGCTAACTCCCGCGTCATCGTGGAAGCTTTCGTGGAATTCGATTACGAGATCACCCTGTTGACAGTAA
GGTCCATCGATCCCACCACCTCTAAGCCTGCGACCTGGTTCTGTGAGCCCATTGGACACCGCCAAGAAGACGGCGACTAC
GTGGAATCCTGGCAGCCAATGGAGATGACTCCTCGCGCGCTGGAAAACGCACGCTCAGTAGCCGCACGCATCACCAACGC
ATTGGGCGGACGCGGCGTATTTGGTGTGGAGCTCTTTGTCTCCGGCGATGACGTGTACTTCTCTGAAGTCTCCCCACGCC
CACACGACACCGGCCTTGTCACCCTTGCCACCCAGCGTTTCTCTGAATTTGAACTCCACGCCAAGGCAATTTTGGGACTG
CCTGTTGATGTCACCCTGATTTCTCCAGGTGCTTCTGCGGTTATTTACGGTGGCATCGAATCTGAAGGCGTGAGCTACAC
CGGTTTGGCTGAAGCACTGGCAGTGGCTGAAACTGATCTTCGTATCTTTGCCAAGCCAGAGGCCTTCACCAAGCGTCGCA
TGGGTGTTGCAGTGTCCACCGCTGAGGATGTGGCTGCTGCCAGGGACCGTGCCACTTTGGCTGCTGCAGCGATCAAGGTT
CATCCAGGAAATTCCGCAGAGGCTTAA

Upstream 100 bases:

>100_bases
AGGTTGCCCCCAAATTTAAGGCATTAGGGTTTAAGCCTTCGGCATGTCAGATAGAGTGAACACCAATATTTCTGACATCT
TCCAACGGAGGCTAAAAGGC

Downstream 100 bases:

>100_bases
CATGCTGGGAACGCATCGGGGCAGCATTTCGTGGGTTTCTACGATCCTGCTTGCCATCGGTGTCATCCTGATCGGATACG
CGGCGGTAGATTTCGGCCCG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MYIPESIGTPLTPNATKVMLLGSGELGKEVAIAFQRLGLEVHAVDRYEHAPAHQVAHFSYVIDMTDAAQVRELVERVRPD
FVIPEIEALATDELVKIEEEGLATIVPTARAAKLTMNREGIRKLAAEELGLPTSNYEFCSTFEEFSAAAEKLGYPNVVKP
VMSSSGKGQSVLRSSDDLQAAWDYAMSGARVANSRVIVEAFVEFDYEITLLTVRSIDPTTSKPATWFCEPIGHRQEDGDY
VESWQPMEMTPRALENARSVAARITNALGGRGVFGVELFVSGDDVYFSEVSPRPHDTGLVTLATQRFSEFELHAKAILGL
PVDVTLISPGASAVIYGGIESEGVSYTGLAEALAVAETDLRIFAKPEAFTKRRMGVAVSTAEDVAAARDRATLAAAAIKV
HPGNSAEA

Sequences:

>Translated_408_residues
MYIPESIGTPLTPNATKVMLLGSGELGKEVAIAFQRLGLEVHAVDRYEHAPAHQVAHFSYVIDMTDAAQVRELVERVRPD
FVIPEIEALATDELVKIEEEGLATIVPTARAAKLTMNREGIRKLAAEELGLPTSNYEFCSTFEEFSAAAEKLGYPNVVKP
VMSSSGKGQSVLRSSDDLQAAWDYAMSGARVANSRVIVEAFVEFDYEITLLTVRSIDPTTSKPATWFCEPIGHRQEDGDY
VESWQPMEMTPRALENARSVAARITNALGGRGVFGVELFVSGDDVYFSEVSPRPHDTGLVTLATQRFSEFELHAKAILGL
PVDVTLISPGASAVIYGGIESEGVSYTGLAEALAVAETDLRIFAKPEAFTKRRMGVAVSTAEDVAAARDRATLAAAAIKV
HPGNSAEA
>Mature_408_residues
MYIPESIGTPLTPNATKVMLLGSGELGKEVAIAFQRLGLEVHAVDRYEHAPAHQVAHFSYVIDMTDAAQVRELVERVRPD
FVIPEIEALATDELVKIEEEGLATIVPTARAAKLTMNREGIRKLAAEELGLPTSNYEFCSTFEEFSAAAEKLGYPNVVKP
VMSSSGKGQSVLRSSDDLQAAWDYAMSGARVANSRVIVEAFVEFDYEITLLTVRSIDPTTSKPATWFCEPIGHRQEDGDY
VESWQPMEMTPRALENARSVAARITNALGGRGVFGVELFVSGDDVYFSEVSPRPHDTGLVTLATQRFSEFELHAKAILGL
PVDVTLISPGASAVIYGGIESEGVSYTGLAEALAVAETDLRIFAKPEAFTKRRMGVAVSTAEDVAAARDRATLAAAAIKV
HPGNSAEA

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=396, Percent_Identity=57.3232323232323, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786733, Length=311, Percent_Identity=24.7588424437299, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=315, Percent_Identity=23.4920634920635, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_CORGB (A4QHG1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139579.1
- ProteinModelPortal:   A4QHG1
- SMR:   A4QHG1
- STRING:   A4QHG1
- GeneID:   4994493
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2662
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44050; Mature: 44050

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 85-85 BINDING 118-118 BINDING 159-159 BINDING 207-207 BINDING 277-277 BINDING 289-289 BINDING 296-296 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIPESIGTPLTPNATKVMLLGSGELGKEVAIAFQRLGLEVHAVDRYEHAPAHQVAHFSY
CCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHEEEEE
VIDMTDAAQVRELVERVRPDFVIPEIEALATDELVKIEEEGLATIVPTARAAKLTMNREG
EEECCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHEEECCCCCEEEECCCHHEEEEECHHH
IRKLAAEELGLPTSNYEFCSTFEEFSAAAEKLGYPNVVKPVMSSSGKGQSVLRSSDDLQA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCHHH
AWDYAMSGARVANSRVIVEAFVEFDYEITLLTVRSIDPTTSKPATWFCEPIGHRQEDGDY
HHHHHHCCCEECCCEEEEEEEHHCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCH
VESWQPMEMTPRALENARSVAARITNALGGRGVFGVELFVSGDDVYFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLATQRFSEFELHAKAILGLPVDVTLISPGASAVIYGGIESEGVSYTGLAEALAVAETDL
EEEHHHHHHHHHEEHHEECCCEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCE
RIFAKPEAFTKRRMGVAVSTAEDVAAARDRATLAAAAIKVHPGNSAEA
EEEECCHHHHHHHCCEEEECHHHHHHHHHHHEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MYIPESIGTPLTPNATKVMLLGSGELGKEVAIAFQRLGLEVHAVDRYEHAPAHQVAHFSY
CCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHEEEEE
VIDMTDAAQVRELVERVRPDFVIPEIEALATDELVKIEEEGLATIVPTARAAKLTMNREG
EEECCCHHHHHHHHHHHCCCCCCCCHHHHCCCHHEEECCCCCEEEECCCHHEEEEECHHH
IRKLAAEELGLPTSNYEFCSTFEEFSAAAEKLGYPNVVKPVMSSSGKGQSVLRSSDDLQA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCHHH
AWDYAMSGARVANSRVIVEAFVEFDYEITLLTVRSIDPTTSKPATWFCEPIGHRQEDGDY
HHHHHHCCCEECCCEEEEEEEHHCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCH
VESWQPMEMTPRALENARSVAARITNALGGRGVFGVELFVSGDDVYFSEVSPRPHDTGLV
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TLATQRFSEFELHAKAILGLPVDVTLISPGASAVIYGGIESEGVSYTGLAEALAVAETDL
EEEHHHHHHHHHEEHHEECCCEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCE
RIFAKPEAFTKRRMGVAVSTAEDVAAARDRATLAAAAIKVHPGNSAEA
EEEECCHHHHHHHCCEEEECHHHHHHHHHHHEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA