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The map label for this gene is ackA

Identifier: 145296751

GI number: 145296751

Start: 2939017

End: 2940210

Strand: Reverse

Name: ackA

Synonym: cgR_2655

Alternate gene names: 145296751

Gene position: 2940210-2939017 (Counterclockwise)

Preceding gene: 145296752

Following gene: 145296747

Centisome position: 88.72

GC content: 50.92

Gene sequence:

>1194_bases
ATGGCATTGGCACTTGTTTTGAACTCCGGTTCATCTTCCATCAAATTCCAGCTGGTCAACCCCGAAAACTCTGCCATCGA
CGAGCCATATGTTTCTGGTCTTGTGGAGCAGATTGGTGAGCCAAACGGCCGCATCGTACTCAAAGTAGAGGGTGAAAAAT
ACACCCTAGAGACACCCATCGCAGATCACTCCGAAGGCCTAAACCTGGCGTTCGATCTCATGGACCAGCACAACTGTGGA
CCTTCCCAACTGGAAATCACCGCAGTCGGACACCGTGTGGTCCACGGCGGAATCTTGTTCTCCGCACCGGAACTTATCAC
TGATGAAATCGTTGAAATGATCCGCGACCTCATTCCACTCGCACCACTGCACAACCCTGCAAACGTTGACGGCATTGATG
TTGCTCGAAAAATTCTCCCCGATGTCCCACACGTAGCTGTCTTTGACACCGGTTTCTTCCACTCACTTCCACCAGCAGCT
GCACTGTATGCCATCAATAAAGATGTTGCCGCTGAACACGGAATTAGGCGCTATGGTTTCCACGGCACCTCCCATGAATT
TGTGTCCAAGCGCGTGGTGGAAATTTTGGAAAAGCCCACCGAAGAAATCAACACCATCACCTTCCACCTGGGAAACGGCG
CATCCATGGCTGCTGTTCAAGGTGGTCGTGCGGTAGATACTTCCATGGGTATGACACCTCTCGCGGGACTTGTCATGGGT
ACCCGAAGCGGTGACATTGATCCAGGTATCGTCTTCCACCTTTCACGCACCGCTGGCATGAGCATCGATGAGATCGATAA
TCTGCTGAACAAAAAGTCGGGTGTAAAGGGACTTTCCGGTGTTAATGATTTCCGTGAACTGCGGGAAATGATCGACAACA
ATGATCAAGATGCCTGGTCCGCCTACAACATTTACATCCACCAGCTTCGCCGCTACCTCGGTTCCTACATGGTGGCACTA
GGACGTGTGGACACCATCGTGTTTACCGCTGGTGTTGGTGAAAATGCACAGTTTGTCCGCGAAGATGCATTGGCGGGATT
GGAAATGTACGGAATTGAGATCGATCCAGAGCGTAACGCATTGCCAAACGATGGTCCTCGATTGATTTCCACTGATGCCT
CCCAGGTGAAGGTGTTTGTCATCCCAACTAATGAAGAATTAGCTATCGCTAGGTACGCGGTGAAGTTCGCTTAG

Upstream 100 bases:

>100_bases
GCCTGAACAAACCAGTCAACGACCTTTCCCGTGGTGCGACAGTCCCTGACATCGTCAACACTGTCGCCATCACCGCTATT
CAGGCAGGAGGACGCAGCTA

Downstream 100 bases:

>100_bases
CTCTCCTGGTTAGGATCCACCACAAATCGCTCTGATCAGCGGTTTTGTGGTGGATTTTTGCGTTTTTAAGGGGTGAAACT
GCACGGATCCACCACAGATC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MALALVLNSGSSSIKFQLVNPENSAIDEPYVSGLVEQIGEPNGRIVLKVEGEKYTLETPIADHSEGLNLAFDLMDQHNCG
PSQLEITAVGHRVVHGGILFSAPELITDEIVEMIRDLIPLAPLHNPANVDGIDVARKILPDVPHVAVFDTGFFHSLPPAA
ALYAINKDVAAEHGIRRYGFHGTSHEFVSKRVVEILEKPTEEINTITFHLGNGASMAAVQGGRAVDTSMGMTPLAGLVMG
TRSGDIDPGIVFHLSRTAGMSIDEIDNLLNKKSGVKGLSGVNDFRELREMIDNNDQDAWSAYNIYIHQLRRYLGSYMVAL
GRVDTIVFTAGVGENAQFVREDALAGLEMYGIEIDPERNALPNDGPRLISTDASQVKVFVIPTNEELAIARYAVKFA

Sequences:

>Translated_397_residues
MALALVLNSGSSSIKFQLVNPENSAIDEPYVSGLVEQIGEPNGRIVLKVEGEKYTLETPIADHSEGLNLAFDLMDQHNCG
PSQLEITAVGHRVVHGGILFSAPELITDEIVEMIRDLIPLAPLHNPANVDGIDVARKILPDVPHVAVFDTGFFHSLPPAA
ALYAINKDVAAEHGIRRYGFHGTSHEFVSKRVVEILEKPTEEINTITFHLGNGASMAAVQGGRAVDTSMGMTPLAGLVMG
TRSGDIDPGIVFHLSRTAGMSIDEIDNLLNKKSGVKGLSGVNDFRELREMIDNNDQDAWSAYNIYIHQLRRYLGSYMVAL
GRVDTIVFTAGVGENAQFVREDALAGLEMYGIEIDPERNALPNDGPRLISTDASQVKVFVIPTNEELAIARYAVKFA
>Mature_396_residues
ALALVLNSGSSSIKFQLVNPENSAIDEPYVSGLVEQIGEPNGRIVLKVEGEKYTLETPIADHSEGLNLAFDLMDQHNCGP
SQLEITAVGHRVVHGGILFSAPELITDEIVEMIRDLIPLAPLHNPANVDGIDVARKILPDVPHVAVFDTGFFHSLPPAAA
LYAINKDVAAEHGIRRYGFHGTSHEFVSKRVVEILEKPTEEINTITFHLGNGASMAAVQGGRAVDTSMGMTPLAGLVMGT
RSGDIDPGIVFHLSRTAGMSIDEIDNLLNKKSGVKGLSGVNDFRELREMIDNNDQDAWSAYNIYIHQLRRYLGSYMVALG
RVDTIVFTAGVGENAQFVREDALAGLEMYGIEIDPERNALPNDGPRLISTDASQVKVFVIPTNEELAIARYAVKFA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=45.5, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI145693184, Length=399, Percent_Identity=40.6015037593985, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_CORGB (A4QHF4)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139572.1
- ProteinModelPortal:   A4QHF4
- SMR:   A4QHF4
- STRING:   A4QHF4
- GeneID:   4994016
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2655
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43093; Mature: 42961

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALALVLNSGSSSIKFQLVNPENSAIDEPYVSGLVEQIGEPNGRIVLKVEGEKYTLETPI
CEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
ADHSEGLNLAFDLMDQHNCGPSQLEITAVGHRVVHGGILFSAPELITDEIVEMIRDLIPL
CCCCCCCCEEEEEHHCCCCCCCEEEEEEECCEEEECCEEECCHHHHHHHHHHHHHHHHCC
APLHNPANVDGIDVARKILPDVPHVAVFDTGFFHSLPPAAALYAINKDVAAEHGIRRYGF
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHEECCCHHHHHCCCHHHCC
HGTSHEFVSKRVVEILEKPTEEINTITFHLGNGASMAAVQGGRAVDTSMGMTPLAGLVMG
CCCCHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECCCCEECCCCCCHHHHHHEEE
TRSGDIDPGIVFHLSRTAGMSIDEIDNLLNKKSGVKGLSGVNDFRELREMIDNNDQDAWS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
AYNIYIHQLRRYLGSYMVALGRVDTIVFTAGVGENAQFVREDALAGLEMYGIEIDPERNA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCC
LPNDGPRLISTDASQVKVFVIPTNEELAIARYAVKFA
CCCCCCEEEECCCCCEEEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
ALALVLNSGSSSIKFQLVNPENSAIDEPYVSGLVEQIGEPNGRIVLKVEGEKYTLETPI
EEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCC
ADHSEGLNLAFDLMDQHNCGPSQLEITAVGHRVVHGGILFSAPELITDEIVEMIRDLIPL
CCCCCCCCEEEEEHHCCCCCCCEEEEEEECCEEEECCEEECCHHHHHHHHHHHHHHHHCC
APLHNPANVDGIDVARKILPDVPHVAVFDTGFFHSLPPAAALYAINKDVAAEHGIRRYGF
CCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHEECCCHHHHHCCCHHHCC
HGTSHEFVSKRVVEILEKPTEEINTITFHLGNGASMAAVQGGRAVDTSMGMTPLAGLVMG
CCCCHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECCCCEECCCCCCHHHHHHEEE
TRSGDIDPGIVFHLSRTAGMSIDEIDNLLNKKSGVKGLSGVNDFRELREMIDNNDQDAWS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
AYNIYIHQLRRYLGSYMVALGRVDTIVFTAGVGENAQFVREDALAGLEMYGIEIDPERNA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCCC
LPNDGPRLISTDASQVKVFVIPTNEELAIARYAVKFA
CCCCCCEEEECCCCCEEEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA