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The map label for this gene is otsA [H]

Identifier: 145296623

GI number: 145296623

Start: 2797029

End: 2798486

Strand: Direct

Name: otsA [H]

Synonym: cgR_2531

Alternate gene names: 145296623

Gene position: 2797029-2798486 (Clockwise)

Preceding gene: 145296622

Following gene: 145296624

Centisome position: 84.4

GC content: 53.98

Gene sequence:

>1458_bases
ATGGATGATTCCAATAGCTTTGTAGTTGTTGCTAACCGTCTGCCAGTGGATATGACTGTCCACCCAGATGGTAGCTATAG
CATCTCCCCCAGCCCCGGTGGCCTTGTCACGGGGCTTTCCCCCGTTCTGGAACAACATCGTGGATGTTGGGTCGGATGGC
CTGGAACTGTAGATGTTGCACCCGAACCATTTCGAACAGATACGGGTGTTTTGCTGCACCCTGTTGTCCTCACTGCAAGT
GACTATGAAGGCTTCTACGAGGGCTTTTCAAACGCAACGCTGTGGCCTCTTTTCCACGATTTGATTGTTACTCCGGTGTA
CAACACCGATTGGTGGCATGCGTTTCGGGAAGTAAACCTCAAGTTCGCTGAAGCCGTGAGCCAAGTGGCGGCACACGGTG
CCACTGTGTGGGTGCAGGACTATCAGCTGTTGCTGGTTCCTGGCATTTTGCGCCAGATGCGCCCTGATTTGAAGATCGGT
TTCTTCCTCCACATTCCCTTCCCTTCCCCTGATCTGTTCCGTCAGCTGCCGTGGCGTGAAGAGATTGTTCGAGGCATGCT
TGGCGCAGATTTGGTGGGATTCCATTTGGTTCAAAACGCAGAAAACTTCCTTGCGTTAACCCAGCAGGTTGCCGGCACTG
CCGGGTCTCATGTGGGTCAGCCGGACACCTTGCAGGTCGGTGGTGAAGCATTGGTGCGTGAGATTGGCGCTCATGTTGAA
ACCGCTGACGGAAGGCGAGTTAGCGTCGGGGCGTTCCCGATCTCGATTGATGTTGAAATGTTTGGGGAGGCGTCGAAAAG
CGCCGTTCTTGATCTTTTAAAAACGCTCGACGAGCCGGAAACCGTATTCCTGGGCGTTGACCGACTGGACTACACCAAGG
GCATTTTGCAGCGCCTGCTTGCGTTTGAGGAACTGCTGGAATCCGGCGCGTTGGAGGCCGACAAAGCTGTGTTGCTGCAG
GTCGCGACGCCTTCGCGTGAGCGCATTGATCACTATCGTGTGTCGCGTTCGCAGGTCGAGGAAGCCGTCGGCCGTATCAA
TGGTCGTTTCGGTCGCATGGGGCGTCCCGTGGTGCATTATCTACACAGGTCATTGAGTAAAAATGATCTCCAGGTGCTGT
ATACCGCAGCCGATGTCATGCTGGTTACGCCTTTTAAAGACGGTATGAACTTGGTGGCTAAAGAATTCGTAGCCAACCAC
CGCGACGGCACTGGTGCTTTGGTGCTGTCCGAATTTGCCGGCGCGGCCACTGAGCTGACCGGTGCGTATTTATGCAACCC
ATTTGATGTGGAATCCATCAAACGGCAAATGGTGGCAGCTGTCCATGATTTGAAGCACAATCCGGAATCTGCGGCAACGC
GAATGAAAACGAACAGCGAGCAGGTCTATACCCACGACGTCAACGTGTGGGCTAATAGTTTCCTGGATTGTTTGGCGCAG
TCGGGAGAAAACTCATGA

Upstream 100 bases:

>100_bases
GAAGGCTGCTGCTAAGAAAACGACCAAGAAGACCACTAAGAAAACTACTAAAAAGACCACCGCAAAGAAGACCACAAAGA
AGTCTTAAGCCGGATCTTAT

Downstream 100 bases:

>100_bases
ACCGCGCACGAATCGCGACCATAGGCGTTCTTCCGCTTGCTTTACTGCTGGCGTCCTGTGGTTCAGACACCGTGGAAATG
ACAGATTCCACCTGGTTGGT

Product: hypothetical protein

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDDSNSFVVVANRLPVDMTVHPDGSYSISPSPGGLVTGLSPVLEQHRGCWVGWPGTVDVAPEPFRTDTGVLLHPVVLTAS
DYEGFYEGFSNATLWPLFHDLIVTPVYNTDWWHAFREVNLKFAEAVSQVAAHGATVWVQDYQLLLVPGILRQMRPDLKIG
FFLHIPFPSPDLFRQLPWREEIVRGMLGADLVGFHLVQNAENFLALTQQVAGTAGSHVGQPDTLQVGGEALVREIGAHVE
TADGRRVSVGAFPISIDVEMFGEASKSAVLDLLKTLDEPETVFLGVDRLDYTKGILQRLLAFEELLESGALEADKAVLLQ
VATPSRERIDHYRVSRSQVEEAVGRINGRFGRMGRPVVHYLHRSLSKNDLQVLYTAADVMLVTPFKDGMNLVAKEFVANH
RDGTGALVLSEFAGAATELTGAYLCNPFDVESIKRQMVAAVHDLKHNPESAATRMKTNSEQVYTHDVNVWANSFLDCLAQ
SGENS

Sequences:

>Translated_485_residues
MDDSNSFVVVANRLPVDMTVHPDGSYSISPSPGGLVTGLSPVLEQHRGCWVGWPGTVDVAPEPFRTDTGVLLHPVVLTAS
DYEGFYEGFSNATLWPLFHDLIVTPVYNTDWWHAFREVNLKFAEAVSQVAAHGATVWVQDYQLLLVPGILRQMRPDLKIG
FFLHIPFPSPDLFRQLPWREEIVRGMLGADLVGFHLVQNAENFLALTQQVAGTAGSHVGQPDTLQVGGEALVREIGAHVE
TADGRRVSVGAFPISIDVEMFGEASKSAVLDLLKTLDEPETVFLGVDRLDYTKGILQRLLAFEELLESGALEADKAVLLQ
VATPSRERIDHYRVSRSQVEEAVGRINGRFGRMGRPVVHYLHRSLSKNDLQVLYTAADVMLVTPFKDGMNLVAKEFVANH
RDGTGALVLSEFAGAATELTGAYLCNPFDVESIKRQMVAAVHDLKHNPESAATRMKTNSEQVYTHDVNVWANSFLDCLAQ
SGENS
>Mature_485_residues
MDDSNSFVVVANRLPVDMTVHPDGSYSISPSPGGLVTGLSPVLEQHRGCWVGWPGTVDVAPEPFRTDTGVLLHPVVLTAS
DYEGFYEGFSNATLWPLFHDLIVTPVYNTDWWHAFREVNLKFAEAVSQVAAHGATVWVQDYQLLLVPGILRQMRPDLKIG
FFLHIPFPSPDLFRQLPWREEIVRGMLGADLVGFHLVQNAENFLALTQQVAGTAGSHVGQPDTLQVGGEALVREIGAHVE
TADGRRVSVGAFPISIDVEMFGEASKSAVLDLLKTLDEPETVFLGVDRLDYTKGILQRLLAFEELLESGALEADKAVLLQ
VATPSRERIDHYRVSRSQVEEAVGRINGRFGRMGRPVVHYLHRSLSKNDLQVLYTAADVMLVTPFKDGMNLVAKEFVANH
RDGTGALVLSEFAGAATELTGAYLCNPFDVESIKRQMVAAVHDLKHNPESAATRMKTNSEQVYTHDVNVWANSFLDCLAQ
SGENS

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=484, Percent_Identity=32.4380165289256, Blast_Score=227, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71987012, Length=580, Percent_Identity=28.2758620689655, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115533558, Length=442, Percent_Identity=30.0904977375566, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI115533560, Length=441, Percent_Identity=30.1587301587302, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6319602, Length=442, Percent_Identity=38.6877828054299, Blast_Score=292, Evalue=8e-80,
Organism=Saccharomyces cerevisiae, GI6323537, Length=413, Percent_Identity=29.2978208232446, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323917, Length=427, Percent_Identity=29.2740046838407, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320279, Length=435, Percent_Identity=29.1954022988506, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI19920676, Length=500, Percent_Identity=36.6, Blast_Score=272, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 53369; Mature: 53369

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSNSFVVVANRLPVDMTVHPDGSYSISPSPGGLVTGLSPVLEQHRGCWVGWPGTVDVA
CCCCCCEEEEEECCCEEEEECCCCCEEECCCCCCHHHCHHHHHHHHCCEEECCCCCCCCC
PEPFRTDTGVLLHPVVLTASDYEGFYEGFSNATLWPLFHDLIVTPVYNTDWWHAFREVNL
CCCCCCCCCEEEEEEEEEECCCHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCH
KFAEAVSQVAAHGATVWVQDYQLLLVPGILRQMRPDLKIGFFLHIPFPSPDLFRQLPWRE
HHHHHHHHHHHCCCEEEEECCHHEEHHHHHHHCCCCCEEEEEEEECCCCHHHHHHCCCHH
EIVRGMLGADLVGFHLVQNAENFLALTQQVAGTAGSHVGQPDTLQVGGEALVREIGAHVE
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCEE
TADGRRVSVGAFPISIDVEMFGEASKSAVLDLLKTLDEPETVFLGVDRLDYTKGILQRLL
CCCCCEEEEEEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
AFEELLESGALEADKAVLLQVATPSRERIDHYRVSRSQVEEAVGRINGRFGRMGRPVVHY
HHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LHRSLSKNDLQVLYTAADVMLVTPFKDGMNLVAKEFVANHRDGTGALVLSEFAGAATELT
HHHHCCCCCEEEEEEEECEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHC
GAYLCNPFDVESIKRQMVAAVHDLKHNPESAATRMKTNSEQVYTHDVNVWANSFLDCLAQ
HHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
SGENS
CCCCC
>Mature Secondary Structure
MDDSNSFVVVANRLPVDMTVHPDGSYSISPSPGGLVTGLSPVLEQHRGCWVGWPGTVDVA
CCCCCCEEEEEECCCEEEEECCCCCEEECCCCCCHHHCHHHHHHHHCCEEECCCCCCCCC
PEPFRTDTGVLLHPVVLTASDYEGFYEGFSNATLWPLFHDLIVTPVYNTDWWHAFREVNL
CCCCCCCCCEEEEEEEEEECCCHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCH
KFAEAVSQVAAHGATVWVQDYQLLLVPGILRQMRPDLKIGFFLHIPFPSPDLFRQLPWRE
HHHHHHHHHHHCCCEEEEECCHHEEHHHHHHHCCCCCEEEEEEEECCCCHHHHHHCCCHH
EIVRGMLGADLVGFHLVQNAENFLALTQQVAGTAGSHVGQPDTLQVGGEALVREIGAHVE
HHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCEE
TADGRRVSVGAFPISIDVEMFGEASKSAVLDLLKTLDEPETVFLGVDRLDYTKGILQRLL
CCCCCEEEEEEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHH
AFEELLESGALEADKAVLLQVATPSRERIDHYRVSRSQVEEAVGRINGRFGRMGRPVVHY
HHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LHRSLSKNDLQVLYTAADVMLVTPFKDGMNLVAKEFVANHRDGTGALVLSEFAGAATELT
HHHHCCCCCEEEEEEEECEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHC
GAYLCNPFDVESIKRQMVAAVHDLKHNPESAATRMKTNSEQVYTHDVNVWANSFLDCLAQ
HHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
SGENS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA