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The map label for this gene is shiA [H]

Identifier: 145296615

GI number: 145296615

Start: 2788610

End: 2789962

Strand: Reverse

Name: shiA [H]

Synonym: cgR_2523

Alternate gene names: 145296615

Gene position: 2789962-2788610 (Counterclockwise)

Preceding gene: 145296616

Following gene: 145296614

Centisome position: 84.18

GC content: 53.58

Gene sequence:

>1353_bases
ATGACTATCAACTCACCAGCGCCGAAGGTCGGTGACACCATGTCCCACGAAGCCTATTCGGCCAAAGCCCATCCAACAAA
AGGCACTCCACAGGCCAAGCGAGCTGCTCTATCAGCATTCCTTGGCTCAACCTTGGAGTACTACGACTTCTTCATTTACG
GCTCGGCAGCTGCACTCGTGTTCTCCCATGTCTTCTTCCCAGAAGGCGGCGCGAACTCAGTTCTGTTGTCTATCTCCACA
CTTGGTGTTGCATATGTTGCTCGCCCAGCAGGTGCGGTCTTGTTCGGTCACCTGGGAGACACCATTGGTCGTCGAAAGAC
TTTGATGATCATCTTGTTCACCATGGGTCTGTCTACCTTGGCTATCGGACTTCTTCCGACTTATGGTCAGGTGGGAATCC
TCGCACCCATCATGCTTGTGGTGCTTCGCCTCCTGCAGGGATTGTCTGCTGGTGGTGAGTCCCCGGGTGCAGCAGCGCTG
TCCATGGAACACGCACCACAGCGTCGTCGCGGTTTCTACTCTTCATTCACCATCTCTGGTGTGATGTTCGGTATCGTGCT
TTCCTCCGTGGTATTCATCCCCATCGCGGCTCTGCCTGATGAACAGCTGTTTAGCTGGGGCTGGCGCATTCCGTTCATCT
TGAGCATCATCTTGACCGGTGTTGCCCTGTGGATCCGTAGGCACCTGGAAGAGTCTGAGACTTTTGAAGAAGAAGTTGAA
GAGGCCAAGGTTCCTGAAACTCCAGTTGTGGAACTGTTCAAGAACCACTGGGCTGCTGTCTTCCGCGTGATGTTCTCCTC
TACTTACGCCATGATGAACACCATCTTGAACGTCTTCGGACTGGCATATGCGGTGTACAGCGACATTGAGCGCACCACCA
TGCTGGGTGTCATCGCGGTGGCAAACCTTATGGCTGTGTTCTCTCAGCCGTTCTTCGGTTTGCTGTCGGACAAGATTGGC
CGCAAGCCAGTGTTCATCACCGGTGCACTTGGTGCTGGAGGCATGATGTTCGTTTTCTTCAATGCGATTAGCACTGGAAA
CATCGCCATGATTTACCTCTCCGCGATGGTTCTGATGGGCCTGTTCTACGCAGCGCCAAACGCTACGTATATGGCAGCGT
TCCCGGAGCAGTTCCCTGCAAAGGTTCGCTACTCAGGAATGGCTATTGGCCTCATGCTTGGCCTGTTGGTCTCAGGATTC
GCACCAGCTGTTGCAGAAATGCTCACTGGTGGACAAGCAGAAAACTGGCAGCCAGTTGCCTGGATGTGCCTTGGTTTCGC
AGTCCTTTCCGCGGTCGCCTTTGCCACAGGTAAAGAAACCTACAAAACCCCAACCCACCTTCTCGGAAAATAA

Upstream 100 bases:

>100_bases
TCCGCCATATAGTTCATTCCATTGGTTATCCAGCTCACAGTTTAGGTTCCAAACGGATAGCCACTTGACCTAAATACCCA
TTCCTTTGAGAGGGAATACG

Downstream 100 bases:

>100_bases
CTCGGCAATTACCCACAACAAAACACTTGAGGGAAGTTTGATTCTCTATGGCTGCATACGCTGTTACCTACGCCTACGTT
CCAGAAAATGATGAGATGGC

Product: hypothetical protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTINSPAPKVGDTMSHEAYSAKAHPTKGTPQAKRAALSAFLGSTLEYYDFFIYGSAAALVFSHVFFPEGGANSVLLSIST
LGVAYVARPAGAVLFGHLGDTIGRRKTLMIILFTMGLSTLAIGLLPTYGQVGILAPIMLVVLRLLQGLSAGGESPGAAAL
SMEHAPQRRRGFYSSFTISGVMFGIVLSSVVFIPIAALPDEQLFSWGWRIPFILSIILTGVALWIRRHLEESETFEEEVE
EAKVPETPVVELFKNHWAAVFRVMFSSTYAMMNTILNVFGLAYAVYSDIERTTMLGVIAVANLMAVFSQPFFGLLSDKIG
RKPVFITGALGAGGMMFVFFNAISTGNIAMIYLSAMVLMGLFYAAPNATYMAAFPEQFPAKVRYSGMAIGLMLGLLVSGF
APAVAEMLTGGQAENWQPVAWMCLGFAVLSAVAFATGKETYKTPTHLLGK

Sequences:

>Translated_450_residues
MTINSPAPKVGDTMSHEAYSAKAHPTKGTPQAKRAALSAFLGSTLEYYDFFIYGSAAALVFSHVFFPEGGANSVLLSIST
LGVAYVARPAGAVLFGHLGDTIGRRKTLMIILFTMGLSTLAIGLLPTYGQVGILAPIMLVVLRLLQGLSAGGESPGAAAL
SMEHAPQRRRGFYSSFTISGVMFGIVLSSVVFIPIAALPDEQLFSWGWRIPFILSIILTGVALWIRRHLEESETFEEEVE
EAKVPETPVVELFKNHWAAVFRVMFSSTYAMMNTILNVFGLAYAVYSDIERTTMLGVIAVANLMAVFSQPFFGLLSDKIG
RKPVFITGALGAGGMMFVFFNAISTGNIAMIYLSAMVLMGLFYAAPNATYMAAFPEQFPAKVRYSGMAIGLMLGLLVSGF
APAVAEMLTGGQAENWQPVAWMCLGFAVLSAVAFATGKETYKTPTHLLGK
>Mature_449_residues
TINSPAPKVGDTMSHEAYSAKAHPTKGTPQAKRAALSAFLGSTLEYYDFFIYGSAAALVFSHVFFPEGGANSVLLSISTL
GVAYVARPAGAVLFGHLGDTIGRRKTLMIILFTMGLSTLAIGLLPTYGQVGILAPIMLVVLRLLQGLSAGGESPGAAALS
MEHAPQRRRGFYSSFTISGVMFGIVLSSVVFIPIAALPDEQLFSWGWRIPFILSIILTGVALWIRRHLEESETFEEEVEE
AKVPETPVVELFKNHWAAVFRVMFSSTYAMMNTILNVFGLAYAVYSDIERTTMLGVIAVANLMAVFSQPFFGLLSDKIGR
KPVFITGALGAGGMMFVFFNAISTGNIAMIYLSAMVLMGLFYAAPNATYMAAFPEQFPAKVRYSGMAIGLMLGLLVSGFA
PAVAEMLTGGQAENWQPVAWMCLGFAVLSAVAFATGKETYKTPTHLLGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=36.1305361305361, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=31.554524361949, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789941, Length=379, Percent_Identity=36.6754617414248, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=30.8252427184466, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=25.3101736972705, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=31.25, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48338; Mature: 48207

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINSPAPKVGDTMSHEAYSAKAHPTKGTPQAKRAALSAFLGSTLEYYDFFIYGSAAALV
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FSHVFFPEGGANSVLLSISTLGVAYVARPAGAVLFGHLGDTIGRRKTLMIILFTMGLSTL
HHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIGLLPTYGQVGILAPIMLVVLRLLQGLSAGGESPGAAALSMEHAPQRRRGFYSSFTISG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCHHHHCCHHHHHHHH
VMFGIVLSSVVFIPIAALPDEQLFSWGWRIPFILSIILTGVALWIRRHLEESETFEEEVE
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKVPETPVVELFKNHWAAVFRVMFSSTYAMMNTILNVFGLAYAVYSDIERTTMLGVIAV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLMAVFSQPFFGLLSDKIGRKPVFITGALGAGGMMFVFFNAISTGNIAMIYLSAMVLMG
HHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
LFYAAPNATYMAAFPEQFPAKVRYSGMAIGLMLGLLVSGFAPAVAEMLTGGQAENWQPVA
HHHHCCCCCEEEECCHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
WMCLGFAVLSAVAFATGKETYKTPTHLLGK
HHHHHHHHHHHHHHHCCCHHCCCCHHHCCC
>Mature Secondary Structure 
TINSPAPKVGDTMSHEAYSAKAHPTKGTPQAKRAALSAFLGSTLEYYDFFIYGSAAALV
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FSHVFFPEGGANSVLLSISTLGVAYVARPAGAVLFGHLGDTIGRRKTLMIILFTMGLSTL
HHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AIGLLPTYGQVGILAPIMLVVLRLLQGLSAGGESPGAAALSMEHAPQRRRGFYSSFTISG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCHHHHCCHHHHHHHH
VMFGIVLSSVVFIPIAALPDEQLFSWGWRIPFILSIILTGVALWIRRHLEESETFEEEVE
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAKVPETPVVELFKNHWAAVFRVMFSSTYAMMNTILNVFGLAYAVYSDIERTTMLGVIAV
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLMAVFSQPFFGLLSDKIGRKPVFITGALGAGGMMFVFFNAISTGNIAMIYLSAMVLMG
HHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
LFYAAPNATYMAAFPEQFPAKVRYSGMAIGLMLGLLVSGFAPAVAEMLTGGQAENWQPVA
HHHHCCCCCEEEECCHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
WMCLGFAVLSAVAFATGKETYKTPTHLLGK
HHHHHHHHHHHHHHHCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]