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The map label for this gene is purB [H]

Identifier: 145296594

GI number: 145296594

Start: 2762549

End: 2763979

Strand: Reverse

Name: purB [H]

Synonym: cgR_2502

Alternate gene names: 145296594

Gene position: 2763979-2762549 (Counterclockwise)

Preceding gene: 145296595

Following gene: 145296593

Centisome position: 83.4

GC content: 57.72

Gene sequence:

>1431_bases
GTGGCTGATAAAAAGAAGATCGCAAACGTCCTGTCGAACCGTTACGCCTCCGCGGAACTTTCTAACCTGTGGAGTGCCGA
AGAGAAGATCATCATGGAGCGCCAGCTCTGGATCGCCGTGATGAAAGCCCAGAAAGATCTGGGAGTTGAGATCCCTGCCG
AGGCAATTGAAGCCTATGAAGCAGTGATCGACCAGGTTGATTTGGCAAGCATCGCCGATCGTGAGCGAGTCACCCGCCAC
GATGTGAAGGCCCGCATCGAAGAATTCAATGCGCTGGCTGGCCATGAGCACATCCACAAGGGCATGACCTCCCGCGACCT
CACGGAAAATGTTGAGCAGCTGCAGATCCACCGCTCCCTTGAGCTGGTCCGCAACAAGGGCATTGCAGTTGTTGCAGCTA
TCGGATCCCGCGCAGCGCAGTACCAAAGCCTGGTCATGGCTGGCCGTTCCCACAACGTGGCAGCGCAGGCAACTACCTTG
GGCAAGCGTTTCGCAACCGCAGCTGATGAAATGCTCGTGGCACTTGAGCGCGTCACCGAACTGCTCAACCGCTACCCACT
TCGCGGAATCAAGGGCCCAATGGGTACCGCTCAAGACATGCTTGACCTCATGGATGGCGACGAAGCTCGTCTTTCCGATC
TGGAAACCCGCATCGCAGCGCACCTCGGCTTTGATCGCGTCTTCGACTCCGTCGGCCAGGTCTACCCACGTTCCCTTGAC
TTCGATGCAGTATCTGCTCTGGTTCAGCTTGGCTCCGGCCCATCATCGCTGTCCCACACCATTCGTCTCATGGCCGGCAC
CGAAACTGTTACCGAAGGTTTCAAGGAGGGACAGGTCGGTTCCTCTGCAATGCCTCACAAGATGAACGCTCGTTCCTGTG
AGCGCGTTGGCGGTCTGCAGGTTATTCTTCGCGGATACCTCACCATGGTTGCTGATCTTTCCGGCCAGCAGTGGAACGAA
GGCGATGTCTTCTGCTCCGTGATCCGCCGCGTTGCACTGCCAGACGCATTCTTCGCGATTGACGGAATGTTTGAAACTTT
CCTGACAGTCCTGGATGAATTCGGTGCATTCCCTGCCATGATCGAGCGCGAACTTGAGCGTTACCTGCCATTCCTGGCAA
CTACCCGTATCCTCATGGCCGCTGTCCGCGCAGGCGTGGGCCGCGAAACCGCACACGAAGTAATCAAGGAAAACGCTGTC
GCGGTTGCCCTCAACATGCGTGAAAACGGCGGTGACCAGGACCTTATCCAGCGTTTGGCTGCTGACGAGCGCCTCCCAAT
GACCGAAGCTGACCTAGAAGCAGCACTGGCTGACCGCCACGCCTTCATTGGCGCTGCGGAGTCCCAGGTATCCCGTGTAC
TCGACCGCATTCAGGCGCTTGTCGACGCCCACCCTGGCGCCGCAGACTACCGACCAGGTGAGATCCTTTAA

Upstream 100 bases:

>100_bases
TCAAAGGAAGACACCATTGAAGGTGTGCGCAAAATCGGAGAATTCATCAAAAAATAGCAGCGACTAGGTTAGTTTCGGTT
CGTGGGGAATAATAATTTGC

Downstream 100 bases:

>100_bases
AGGTTTTTAACGGCGTCCACGCGGGTTTACCGCTGGATCGCAGTGAGAAAATGGGGCCTCGAGCCCCATTTTTTGTACCT
AAAGCATTTGTGCAGCTCGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MADKKKIANVLSNRYASAELSNLWSAEEKIIMERQLWIAVMKAQKDLGVEIPAEAIEAYEAVIDQVDLASIADRERVTRH
DVKARIEEFNALAGHEHIHKGMTSRDLTENVEQLQIHRSLELVRNKGIAVVAAIGSRAAQYQSLVMAGRSHNVAAQATTL
GKRFATAADEMLVALERVTELLNRYPLRGIKGPMGTAQDMLDLMDGDEARLSDLETRIAAHLGFDRVFDSVGQVYPRSLD
FDAVSALVQLGSGPSSLSHTIRLMAGTETVTEGFKEGQVGSSAMPHKMNARSCERVGGLQVILRGYLTMVADLSGQQWNE
GDVFCSVIRRVALPDAFFAIDGMFETFLTVLDEFGAFPAMIERELERYLPFLATTRILMAAVRAGVGRETAHEVIKENAV
AVALNMRENGGDQDLIQRLAADERLPMTEADLEAALADRHAFIGAAESQVSRVLDRIQALVDAHPGAADYRPGEIL

Sequences:

>Translated_476_residues
MADKKKIANVLSNRYASAELSNLWSAEEKIIMERQLWIAVMKAQKDLGVEIPAEAIEAYEAVIDQVDLASIADRERVTRH
DVKARIEEFNALAGHEHIHKGMTSRDLTENVEQLQIHRSLELVRNKGIAVVAAIGSRAAQYQSLVMAGRSHNVAAQATTL
GKRFATAADEMLVALERVTELLNRYPLRGIKGPMGTAQDMLDLMDGDEARLSDLETRIAAHLGFDRVFDSVGQVYPRSLD
FDAVSALVQLGSGPSSLSHTIRLMAGTETVTEGFKEGQVGSSAMPHKMNARSCERVGGLQVILRGYLTMVADLSGQQWNE
GDVFCSVIRRVALPDAFFAIDGMFETFLTVLDEFGAFPAMIERELERYLPFLATTRILMAAVRAGVGRETAHEVIKENAV
AVALNMRENGGDQDLIQRLAADERLPMTEADLEAALADRHAFIGAAESQVSRVLDRIQALVDAHPGAADYRPGEIL
>Mature_475_residues
ADKKKIANVLSNRYASAELSNLWSAEEKIIMERQLWIAVMKAQKDLGVEIPAEAIEAYEAVIDQVDLASIADRERVTRHD
VKARIEEFNALAGHEHIHKGMTSRDLTENVEQLQIHRSLELVRNKGIAVVAAIGSRAAQYQSLVMAGRSHNVAAQATTLG
KRFATAADEMLVALERVTELLNRYPLRGIKGPMGTAQDMLDLMDGDEARLSDLETRIAAHLGFDRVFDSVGQVYPRSLDF
DAVSALVQLGSGPSSLSHTIRLMAGTETVTEGFKEGQVGSSAMPHKMNARSCERVGGLQVILRGYLTMVADLSGQQWNEG
DVFCSVIRRVALPDAFFAIDGMFETFLTVLDEFGAFPAMIERELERYLPFLATTRILMAAVRAGVGRETAHEVIKENAVA
VALNMRENGGDQDLIQRLAADERLPMTEADLEAALADRHAFIGAAESQVSRVLDRIQALVDAHPGAADYRPGEIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=32.2222222222222, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=32.03125, Blast_Score=206, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17508577, Length=408, Percent_Identity=29.4117647058824, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI32564234, Length=355, Percent_Identity=30.4225352112676, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323391, Length=461, Percent_Identity=32.9718004338395, Blast_Score=244, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24647570, Length=442, Percent_Identity=31.9004524886878, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52192; Mature: 52061

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKKKIANVLSNRYASAELSNLWSAEEKIIMERQLWIAVMKAQKDLGVEIPAEAIEAYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AVIDQVDLASIADRERVTRHDVKARIEEFNALAGHEHIHKGMTSRDLTENVEQLQIHRSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELVRNKGIAVVAAIGSRAAQYQSLVMAGRSHNVAAQATTLGKRFATAADEMLVALERVTE
HHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNRYPLRGIKGPMGTAQDMLDLMDGDEARLSDLETRIAAHLGFDRVFDSVGQVYPRSLD
HHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDAVSALVQLGSGPSSLSHTIRLMAGTETVTEGFKEGQVGSSAMPHKMNARSCERVGGLQ
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VILRGYLTMVADLSGQQWNEGDVFCSVIRRVALPDAFFAIDGMFETFLTVLDEFGAFPAM
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IERELERYLPFLATTRILMAAVRAGVGRETAHEVIKENAVAVALNMRENGGDQDLIQRLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ADERLPMTEADLEAALADRHAFIGAAESQVSRVLDRIQALVDAHPGAADYRPGEIL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADKKKIANVLSNRYASAELSNLWSAEEKIIMERQLWIAVMKAQKDLGVEIPAEAIEAYE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AVIDQVDLASIADRERVTRHDVKARIEEFNALAGHEHIHKGMTSRDLTENVEQLQIHRSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELVRNKGIAVVAAIGSRAAQYQSLVMAGRSHNVAAQATTLGKRFATAADEMLVALERVTE
HHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNRYPLRGIKGPMGTAQDMLDLMDGDEARLSDLETRIAAHLGFDRVFDSVGQVYPRSLD
HHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDAVSALVQLGSGPSSLSHTIRLMAGTETVTEGFKEGQVGSSAMPHKMNARSCERVGGLQ
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VILRGYLTMVADLSGQQWNEGDVFCSVIRRVALPDAFFAIDGMFETFLTVLDEFGAFPAM
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IERELERYLPFLATTRILMAAVRAGVGRETAHEVIKENAVAVALNMRENGGDQDLIQRLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ADERLPMTEADLEAALADRHAFIGAAESQVSRVLDRIQALVDAHPGAADYRPGEIL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]