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The map label for this gene is dctA [H]

Identifier: 145296589

GI number: 145296589

Start: 2756874

End: 2758214

Strand: Reverse

Name: dctA [H]

Synonym: cgR_2497

Alternate gene names: 145296589

Gene position: 2758214-2756874 (Counterclockwise)

Preceding gene: 145296590

Following gene: 145296588

Centisome position: 83.22

GC content: 51.45

Gene sequence:

>1341_bases
ATGGATTCAAACACAGAATCTTCAAGTGTTGAGGTCAAAAACGAACACATTAAAGTTCAAAAGCCGCCGAAGAAGGACCG
CACTCACTGGCTCTACATTGCGGTTATCATCGCATTGATTGGCGGTATTACCCTAGGTCTGGTTTCGCCGGAATTGGGCA
AAGAATTCAAGATTTTGGGCACCATGTTTGTGTCCTTGATCAAGATGATTATCGCTCCAGTTATTTTCTGCACCATCGTC
ATCGGAATCGGTTCAGTTAAGGCAGCGGCAACAGTCGGACGCGCTGGTGGCATCGCCCTTGCGTACTTCATCACGATGTC
CACATTCGCACTCGCAGTTGGCCTGCTAGTCGGTAACTTCATCCAGCCAGGTAGCGGACTGAACATCTCAGTTGATGAAG
AATCTTCATTCGCATCCACAGAGAGCAGCCCTGAAGGACTCTTGGGATTCATCCACTCGATCATCCCTGAAACGTTCTTC
TCTGCATTTACTGATGGTTCGGTGCTGCAGGTACTGTTCATCGCCATCCTCGTGGGCTTTGCAGCTCAGTCGATGGGTGA
AAAGGGACAGCCCATCCTTGATTTCGTTTCCCATCTGCAGAAGCTCATCTTCAAGATTTTGAACTGGATTCTGTGGCTCG
CCCCAGTCGGTGCATTCGGTGCAATGGCCGGCGTTGTTGGCGAAACAGGCTTTGATGCCGTTGTTCAGCTCGGTATTTTG
ATCCTTGCCTTTTACGTCACCTGCGCGATCTTCATCTTTGGCGTGCTGGGCGCCGTACTGAAGGTGTTCACCGGCGTGAA
CATCTTCAAGCTGGTCAAGTACCTTGCCAAGGAATTCCTGCTGATCTTTGCTACCTCATCCTCTGAATCTGCCTTGCCAA
ACCTCATGCGCAAGATGGAACACATCGGTGTGGCTAAACCAACCGTCGGAATCGTGGTCCCAACCGGCTATTCCTTCAAC
TTGGACGGCACCGCAATTTACCTCACCATGGCATCCATATTCATTGCCGACGCGATGAATATGCCGATGAGCCTCGGCGA
GCAGGTCGGTCTGCTTGTCTTCATGATCATCGCATCCAAGGGTGCTGCTGGTGTCTCGGGTGCAGGTATCGCAACTCTGG
CTGCCGGATTGTCCTCACACCGCCCAGAACTTCTGCACGGCGTTGACGTGATTGTGGGCATCGATAAATTCATGTCTGAA
GCCCGCGCACTAACCAACTTCGCCGGAAACTCCGTGGCAACACTGCTGGTCGGCAAGTGGACTGGCACCGTGGACATGAA
CCAAGTCCATGACGTTTTGAATGGAAAATCTCCATTTGTGGAGTTAGAGGAAGAGCACTAG

Upstream 100 bases:

>100_bases
ATTTAGGCTTCGACGGAAGCGATCACACCACGTTAAATTTCGACCATCAATAACGGTGGCCGTTTTTTGGCGAATCCCCA
AAAGGTCGAAGAGGAAGTTC

Downstream 100 bases:

>100_bases
TTTTCAACAGGACGACAACGGCCGGACATGCGACAATACAATGCATGTCCGGCCGTCTTCTTGTTTCAGTTTCTAGTATT
TTCGACCAGACCCGATCGGC

Product: Na+/H+-dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDSNTESSSVEVKNEHIKVQKPPKKDRTHWLYIAVIIALIGGITLGLVSPELGKEFKILGTMFVSLIKMIIAPVIFCTIV
IGIGSVKAAATVGRAGGIALAYFITMSTFALAVGLLVGNFIQPGSGLNISVDEESSFASTESSPEGLLGFIHSIIPETFF
SAFTDGSVLQVLFIAILVGFAAQSMGEKGQPILDFVSHLQKLIFKILNWILWLAPVGAFGAMAGVVGETGFDAVVQLGIL
ILAFYVTCAIFIFGVLGAVLKVFTGVNIFKLVKYLAKEFLLIFATSSSESALPNLMRKMEHIGVAKPTVGIVVPTGYSFN
LDGTAIYLTMASIFIADAMNMPMSLGEQVGLLVFMIIASKGAAGVSGAGIATLAAGLSSHRPELLHGVDVIVGIDKFMSE
ARALTNFAGNSVATLLVGKWTGTVDMNQVHDVLNGKSPFVELEEEH

Sequences:

>Translated_446_residues
MDSNTESSSVEVKNEHIKVQKPPKKDRTHWLYIAVIIALIGGITLGLVSPELGKEFKILGTMFVSLIKMIIAPVIFCTIV
IGIGSVKAAATVGRAGGIALAYFITMSTFALAVGLLVGNFIQPGSGLNISVDEESSFASTESSPEGLLGFIHSIIPETFF
SAFTDGSVLQVLFIAILVGFAAQSMGEKGQPILDFVSHLQKLIFKILNWILWLAPVGAFGAMAGVVGETGFDAVVQLGIL
ILAFYVTCAIFIFGVLGAVLKVFTGVNIFKLVKYLAKEFLLIFATSSSESALPNLMRKMEHIGVAKPTVGIVVPTGYSFN
LDGTAIYLTMASIFIADAMNMPMSLGEQVGLLVFMIIASKGAAGVSGAGIATLAAGLSSHRPELLHGVDVIVGIDKFMSE
ARALTNFAGNSVATLLVGKWTGTVDMNQVHDVLNGKSPFVELEEEH
>Mature_446_residues
MDSNTESSSVEVKNEHIKVQKPPKKDRTHWLYIAVIIALIGGITLGLVSPELGKEFKILGTMFVSLIKMIIAPVIFCTIV
IGIGSVKAAATVGRAGGIALAYFITMSTFALAVGLLVGNFIQPGSGLNISVDEESSFASTESSPEGLLGFIHSIIPETFF
SAFTDGSVLQVLFIAILVGFAAQSMGEKGQPILDFVSHLQKLIFKILNWILWLAPVGAFGAMAGVVGETGFDAVVQLGIL
ILAFYVTCAIFIFGVLGAVLKVFTGVNIFKLVKYLAKEFLLIFATSSSESALPNLMRKMEHIGVAKPTVGIVVPTGYSFN
LDGTAIYLTMASIFIADAMNMPMSLGEQVGLLVFMIIASKGAAGVSGAGIATLAAGLSSHRPELLHGVDVIVGIDKFMSE
ARALTNFAGNSVATLLVGKWTGTVDMNQVHDVLNGKSPFVELEEEH

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=379, Percent_Identity=28.2321899736148, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI169790839, Length=462, Percent_Identity=25.7575757575758, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI194239697, Length=458, Percent_Identity=24.235807860262, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI40254478, Length=477, Percent_Identity=23.2704402515723, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4827012, Length=232, Percent_Identity=32.3275862068966, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI66773030, Length=491, Percent_Identity=23.8289205702648, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI5032093, Length=430, Percent_Identity=26.046511627907, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI223468566, Length=262, Percent_Identity=26.7175572519084, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI262359914, Length=442, Percent_Identity=24.8868778280543, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI223468564, Length=262, Percent_Identity=26.7175572519084, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=49.0338164251208, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1790514, Length=377, Percent_Identity=33.42175066313, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788024, Length=397, Percent_Identity=24.1813602015113, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789473, Length=329, Percent_Identity=24.0121580547112, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=433, Percent_Identity=24.9422632794457, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=467, Percent_Identity=25.4817987152034, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983099, Length=464, Percent_Identity=24.1379310344828, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71983106, Length=464, Percent_Identity=24.1379310344828, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=451, Percent_Identity=22.1729490022173, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=431, Percent_Identity=22.0417633410673, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17541374, Length=400, Percent_Identity=21.5, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583025, Length=411, Percent_Identity=25.3041362530414, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17137668, Length=411, Percent_Identity=25.3041362530414, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24583023, Length=411, Percent_Identity=25.3041362530414, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281360481, Length=350, Percent_Identity=26.2857142857143, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281360483, Length=350, Percent_Identity=26.2857142857143, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137666, Length=350, Percent_Identity=26.2857142857143, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47209; Mature: 47209

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNTESSSVEVKNEHIKVQKPPKKDRTHWLYIAVIIALIGGITLGLVSPELGKEFKILG
CCCCCCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHH
TMFVSLIKMIIAPVIFCTIVIGIGSVKAAATVGRAGGIALAYFITMSTFALAVGLLVGNF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
IQPGSGLNISVDEESSFASTESSPEGLLGFIHSIIPETFFSAFTDGSVLQVLFIAILVGF
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AAQSMGEKGQPILDFVSHLQKLIFKILNWILWLAPVGAFGAMAGVVGETGFDAVVQLGIL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ILAFYVTCAIFIFGVLGAVLKVFTGVNIFKLVKYLAKEFLLIFATSSSESALPNLMRKME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
HIGVAKPTVGIVVPTGYSFNLDGTAIYLTMASIFIADAMNMPMSLGEQVGLLVFMIIASK
HCCCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GAAGVSGAGIATLAAGLSSHRPELLHGVDVIVGIDKFMSEARALTNFAGNSVATLLVGKW
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
TGTVDMNQVHDVLNGKSPFVELEEEH
CCCCCHHHHHHHHCCCCCCEECCCCC
>Mature Secondary Structure
MDSNTESSSVEVKNEHIKVQKPPKKDRTHWLYIAVIIALIGGITLGLVSPELGKEFKILG
CCCCCCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHH
TMFVSLIKMIIAPVIFCTIVIGIGSVKAAATVGRAGGIALAYFITMSTFALAVGLLVGNF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
IQPGSGLNISVDEESSFASTESSPEGLLGFIHSIIPETFFSAFTDGSVLQVLFIAILVGF
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AAQSMGEKGQPILDFVSHLQKLIFKILNWILWLAPVGAFGAMAGVVGETGFDAVVQLGIL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ILAFYVTCAIFIFGVLGAVLKVFTGVNIFKLVKYLAKEFLLIFATSSSESALPNLMRKME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
HIGVAKPTVGIVVPTGYSFNLDGTAIYLTMASIFIADAMNMPMSLGEQVGLLVFMIIASK
HCCCCCCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GAAGVSGAGIATLAAGLSSHRPELLHGVDVIVGIDKFMSEARALTNFAGNSVATLLVGKW
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
TGTVDMNQVHDVLNGKSPFVELEEEH
CCCCCHHHHHHHHCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA