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The map label for this gene is glsA [H]

Identifier: 145296480

GI number: 145296480

Start: 2628273

End: 2629589

Strand: Reverse

Name: glsA [H]

Synonym: cgR_2392

Alternate gene names: 145296480

Gene position: 2629589-2628273 (Counterclockwise)

Preceding gene: 145296490

Following gene: 145296479

Centisome position: 79.34

GC content: 57.63

Gene sequence:

>1317_bases
TTGAGTCGCTGTGAGCCCCAGAATCTGTCATGCACAAGAGTATATAGCGCAAAAGAAATCACTAGTCTTGATTCTATGTT
GACGATGCCGATACCCGAGTACCTGCACGAAATTTTAGATGATGTCCGCGACACCACCTCCGGCGAGTTGGCCGATTACA
TCCCGGAACTAAAATCTGCGGACCCAAACCCGCTGGCAGTAGCCCTGTGCACCGTCAACGGACACATTTACAGCGCGGGC
GACGACGACGTGGCATTCACCATGCAAAGTATTTCCAAGCCCTTTGCCTACGCACTCGCACTCCAAGAATGCGGCTTTGA
TGAGGTCTCTGCATCCGTGGCCTTGGAACCCTCCGGTGAGGCCTTCAACGAACTTTCCCTCGACGGCGAAAACCGCCCCA
TGAACCCCATGATCAACGCCGGTGCGATCGCCATCAACCAGCTGATCAACGGCTCCGATTCCACCGTGGAAGACCGCGTG
GAAAAAATCCGACAGTACTTCTCTGAACTTGCTGGACGCGAACTTACCATCGACCGCGTGCTTGCCGAATCCGAACTCGC
CGGCGCCGACCGCAACCTCTCCATCGCCCACATGCTGCGCAACTATGGCGTCATCGAAGACGAAGCCCACGACGCCGTCC
TCAGCTACACGCTGCAATGCGCCATCAAAGTAACCACGCGCGACCTCGCAGTCATGACCGCCACGCTCGCCGCCGGCGGC
ACGCACCCAATTACCGGCAAGAAGCTTCTCGACGCCCGCGTCTGCCGCCTCACCCTCTCCGTCATGGCTTCAGCAGGCAT
GTACGACGAGGCAGGGCAGTGGCTCTCCACCGTAGGCATCCCCGCGAAATCAGGAGTCGCCGGCGGACTCATCGGCATTC
TGCCAGGTCAGCTGGGCATCGCCACATTTTCCCCACGCCTGAACCCCAAAGGCAACAGCGTGCGCGGCGTAGAAATATTC
AAACAGCTTTCCGACGACATGGGCCTCCACCTCATGTCCACCGAGCAGGTATCCGGCCACGCAGTACGATCCATCACGCG
GGACGGCGACACCACCTTCATCCAAATGCAGGGCGCCATGAACTTCTCAGCCAGCGAAAGCTTCCTCCACGCCATCGTGG
AACACAACTTTGAAGGTACCGAAGTTGTTCTTGATCTCACCCGAGTACTTAGCTTCCACCCCGTAGCCATCCGCATGATC
AAAGAAGGCCTCAAACGCATCCGCGACGCAGGCTTTGAGGTGTTCATCCTCGACCCAGATGGCGTACTGCCCGATTTCAT
GTTTTCCGACGGCACCATCTGCAAAGAACGAGTGTGA

Upstream 100 bases:

>100_bases
TCCGCAGTGGAAAAACTCACTCGCCCAGGCTGCGAAAACGCCCGCGACACCGTGGAAGGGGAGACACCAGCGACTTTTGC
GACATCATAAATGGTGGCTT

Downstream 100 bases:

>100_bases
CCGGTAGCTTTATGGTCTGAACAATTCTAAGGAGATTAATCGGTGAAAAAGAAGATTATGTTGCCTTTGATTGTTGCAGC
TTTGGGTTAAGTGCCTGCAG

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSRCEPQNLSCTRVYSAKEITSLDSMLTMPIPEYLHEILDDVRDTTSGELADYIPELKSADPNPLAVALCTVNGHIYSAG
DDDVAFTMQSISKPFAYALALQECGFDEVSASVALEPSGEAFNELSLDGENRPMNPMINAGAIAINQLINGSDSTVEDRV
EKIRQYFSELAGRELTIDRVLAESELAGADRNLSIAHMLRNYGVIEDEAHDAVLSYTLQCAIKVTTRDLAVMTATLAAGG
THPITGKKLLDARVCRLTLSVMASAGMYDEAGQWLSTVGIPAKSGVAGGLIGILPGQLGIATFSPRLNPKGNSVRGVEIF
KQLSDDMGLHLMSTEQVSGHAVRSITRDGDTTFIQMQGAMNFSASESFLHAIVEHNFEGTEVVLDLTRVLSFHPVAIRMI
KEGLKRIRDAGFEVFILDPDGVLPDFMFSDGTICKERV

Sequences:

>Translated_438_residues
MSRCEPQNLSCTRVYSAKEITSLDSMLTMPIPEYLHEILDDVRDTTSGELADYIPELKSADPNPLAVALCTVNGHIYSAG
DDDVAFTMQSISKPFAYALALQECGFDEVSASVALEPSGEAFNELSLDGENRPMNPMINAGAIAINQLINGSDSTVEDRV
EKIRQYFSELAGRELTIDRVLAESELAGADRNLSIAHMLRNYGVIEDEAHDAVLSYTLQCAIKVTTRDLAVMTATLAAGG
THPITGKKLLDARVCRLTLSVMASAGMYDEAGQWLSTVGIPAKSGVAGGLIGILPGQLGIATFSPRLNPKGNSVRGVEIF
KQLSDDMGLHLMSTEQVSGHAVRSITRDGDTTFIQMQGAMNFSASESFLHAIVEHNFEGTEVVLDLTRVLSFHPVAIRMI
KEGLKRIRDAGFEVFILDPDGVLPDFMFSDGTICKERV
>Mature_437_residues
SRCEPQNLSCTRVYSAKEITSLDSMLTMPIPEYLHEILDDVRDTTSGELADYIPELKSADPNPLAVALCTVNGHIYSAGD
DDVAFTMQSISKPFAYALALQECGFDEVSASVALEPSGEAFNELSLDGENRPMNPMINAGAIAINQLINGSDSTVEDRVE
KIRQYFSELAGRELTIDRVLAESELAGADRNLSIAHMLRNYGVIEDEAHDAVLSYTLQCAIKVTTRDLAVMTATLAAGGT
HPITGKKLLDARVCRLTLSVMASAGMYDEAGQWLSTVGIPAKSGVAGGLIGILPGQLGIATFSPRLNPKGNSVRGVEIFK
QLSDDMGLHLMSTEQVSGHAVRSITRDGDTTFIQMQGAMNFSASESFLHAIVEHNFEGTEVVLDLTRVLSFHPVAIRMIK
EGLKRIRDAGFEVFILDPDGVLPDFMFSDGTICKERV

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain [H]

Homologues:

Organism=Homo sapiens, GI156104878, Length=306, Percent_Identity=38.2352941176471, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI20336214, Length=300, Percent_Identity=38, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1786693, Length=288, Percent_Identity=39.2361111111111, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787804, Length=297, Percent_Identity=34.3434343434343, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17532727, Length=312, Percent_Identity=40.0641025641026, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17507019, Length=356, Percent_Identity=37.0786516853933, Blast_Score=231, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI193204073, Length=313, Percent_Identity=37.6996805111821, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI193204075, Length=315, Percent_Identity=37.4603174603175, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24653162, Length=356, Percent_Identity=34.2696629213483, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI281363241, Length=356, Percent_Identity=34.2696629213483, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI24653164, Length=356, Percent_Identity=34.2696629213483, Blast_Score=212, Evalue=5e-55,
Organism=Drosophila melanogaster, GI116008307, Length=356, Percent_Identity=34.2696629213483, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24653156, Length=356, Percent_Identity=34.2696629213483, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24653158, Length=356, Percent_Identity=34.2696629213483, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI281363239, Length=366, Percent_Identity=33.879781420765, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24653166, Length=356, Percent_Identity=34.2696629213483, Blast_Score=211, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015868
- InterPro:   IPR002645 [H]

Pfam domain/function: PF04960 Glutaminase [H]

EC number: =3.5.1.2 [H]

Molecular weight: Translated: 47411; Mature: 47280

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCEPQNLSCTRVYSAKEITSLDSMLTMPIPEYLHEILDDVRDTTSGELADYIPELKSA
CCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
DPNPLAVALCTVNGHIYSAGDDDVAFTMQSISKPFAYALALQECGFDEVSASVALEPSGE
CCCCEEEEEEEECCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHCCEEEECCCCC
AFNELSLDGENRPMNPMINAGAIAINQLINGSDSTVEDRVEKIRQYFSELAGRELTIDRV
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHH
LAESELAGADRNLSIAHMLRNYGVIEDEAHDAVLSYTLQCAIKVTTRDLAVMTATLAAGG
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEEEEEEEEEECHHHHHHHHHHHCCC
THPITGKKLLDARVCRLTLSVMASAGMYDEAGQWLSTVGIPAKSGVAGGLIGILPGQLGI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCE
ATFSPRLNPKGNSVRGVEIFKQLSDDMGLHLMSTEQVSGHAVRSITRDGDTTFIQMQGAM
EEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCC
NFSASESFLHAIVEHNFEGTEVVLDLTRVLSFHPVAIRMIKEGLKRIRDAGFEVFILDPD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECCC
GVLPDFMFSDGTICKERV
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRCEPQNLSCTRVYSAKEITSLDSMLTMPIPEYLHEILDDVRDTTSGELADYIPELKSA
CCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
DPNPLAVALCTVNGHIYSAGDDDVAFTMQSISKPFAYALALQECGFDEVSASVALEPSGE
CCCCEEEEEEEECCEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHCCEEEECCCCC
AFNELSLDGENRPMNPMINAGAIAINQLINGSDSTVEDRVEKIRQYFSELAGRELTIDRV
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHH
LAESELAGADRNLSIAHMLRNYGVIEDEAHDAVLSYTLQCAIKVTTRDLAVMTATLAAGG
HHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEEEEEEEEEECHHHHHHHHHHHCCC
THPITGKKLLDARVCRLTLSVMASAGMYDEAGQWLSTVGIPAKSGVAGGLIGILPGQLGI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCE
ATFSPRLNPKGNSVRGVEIFKQLSDDMGLHLMSTEQVSGHAVRSITRDGDTTFIQMQGAM
EEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCC
NFSASESFLHAIVEHNFEGTEVVLDLTRVLSFHPVAIRMIKEGLKRIRDAGFEVFILDPD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEECCC
GVLPDFMFSDGTICKERV
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]