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The map label for this gene is pncA [C]

Identifier: 145296462

GI number: 145296462

Start: 2610932

End: 2611558

Strand: Reverse

Name: pncA [C]

Synonym: cgR_2375

Alternate gene names: 145296462

Gene position: 2611558-2610932 (Counterclockwise)

Preceding gene: 145296463

Following gene: 145296460

Centisome position: 78.8

GC content: 56.46

Gene sequence:

>627_bases
ATGTCTACACCACGTCGTGCACTAGTAATCATCGATGTCCAGCAGGAATACTTCGAAGGCTTGCTGCCCATTCAGTACCC
AAACCGCGATGAGTCCATCGCTCGAATCGCCGCAGCCATGGATGCTGCCTCAAGCGCTGACGTGCCGGTGGTCGTCGTCA
AGCATGAAATGCCCGAAGGTGCTCCGATCTTCGTGCCAGGCACAGCAACGTTCCCGCTTCACCCAGAATTGGACAAGCGT
TCAGATAAGGTTTCACATGAACTGACCAAGATCTATGCCAGCATCTTCGCTGACACTGATCTGGAGCAGTGGCTGCGTGA
GCGAAACATCGACACCATCACCCTCGTTGGCTACATGACCAACAATTGCGTCATCGGTTCCGCGGCAGCTGCCGAGCCTC
TGGGCTTCAAGGTGGAAGTGCTTTCCGACGCCACCGGCGCCATCGATCTGGTCAACGCAGCGGGTTCCGCGCCAGCCAAG
CAGGTTCATGAAACCATCATGGCGTTGCTGAACTCTAACTGGGCTGCAGTCACCACCACCGAGGGTTGGATCGAAGCTGT
TTCCTCCAAGACTGCACTGGAAGGGGACAACCTCATCGAATCTGCGGCGCGAGGCTGTAACGCGTAA

Upstream 100 bases:

>100_bases
GTTCTTGGCACTCACACACCTGGGGTCGATGCCCACGGCCTTGAGCTATTGGCAGATCACTACAACCTTTCCTAATTTCA
CATCTCAGAAGGAGTTTCAC

Downstream 100 bases:

>100_bases
AAGTTCGTAAATGGGCCAGTCTGCAAGAAAAAACATATGCTGATTTATACGTTTGGTTATTTGCCGACTGGCCCTTTTGA
TCTAGCTGACGAGGATATCG

Product: hypothetical protein

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSTPRRALVIIDVQQEYFEGLLPIQYPNRDESIARIAAAMDAASSADVPVVVVKHEMPEGAPIFVPGTATFPLHPELDKR
SDKVSHELTKIYASIFADTDLEQWLRERNIDTITLVGYMTNNCVIGSAAAAEPLGFKVEVLSDATGAIDLVNAAGSAPAK
QVHETIMALLNSNWAAVTTTEGWIEAVSSKTALEGDNLIESAARGCNA

Sequences:

>Translated_208_residues
MSTPRRALVIIDVQQEYFEGLLPIQYPNRDESIARIAAAMDAASSADVPVVVVKHEMPEGAPIFVPGTATFPLHPELDKR
SDKVSHELTKIYASIFADTDLEQWLRERNIDTITLVGYMTNNCVIGSAAAAEPLGFKVEVLSDATGAIDLVNAAGSAPAK
QVHETIMALLNSNWAAVTTTEGWIEAVSSKTALEGDNLIESAARGCNA
>Mature_207_residues
STPRRALVIIDVQQEYFEGLLPIQYPNRDESIARIAAAMDAASSADVPVVVVKHEMPEGAPIFVPGTATFPLHPELDKRS
DKVSHELTKIYASIFADTDLEQWLRERNIDTITLVGYMTNNCVIGSAAAAEPLGFKVEVLSDATGAIDLVNAAGSAPAKQ
VHETIMALLNSNWAAVTTTEGWIEAVSSKTALEGDNLIESAARGCNA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 22293; Mature: 22162

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPRRALVIIDVQQEYFEGLLPIQYPNRDESIARIAAAMDAASSADVPVVVVKHEMPEG
CCCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
APIFVPGTATFPLHPELDKRSDKVSHELTKIYASIFADTDLEQWLRERNIDTITLVGYMT
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEE
NNCVIGSAAAAEPLGFKVEVLSDATGAIDLVNAAGSAPAKQVHETIMALLNSNWAAVTTT
CCEEEECCCCCCCCCEEEEEECCCCCCEEEHHCCCCCCHHHHHHHHHHHHCCCCEEEEEC
EGWIEAVSSKTALEGDNLIESAARGCNA
HHHHHHHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
STPRRALVIIDVQQEYFEGLLPIQYPNRDESIARIAAAMDAASSADVPVVVVKHEMPEG
CCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
APIFVPGTATFPLHPELDKRSDKVSHELTKIYASIFADTDLEQWLRERNIDTITLVGYMT
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEE
NNCVIGSAAAAEPLGFKVEVLSDATGAIDLVNAAGSAPAKQVHETIMALLNSNWAAVTTT
CCEEEECCCCCCCCCEEEEEECCCCCCEEEHHCCCCCCHHHHHHHHHHHHCCCCEEEEEC
EGWIEAVSSKTALEGDNLIESAARGCNA
HHHHHHHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA