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The map label for this gene is proB [H]

Identifier: 145296324

GI number: 145296324

Start: 2468382

End: 2469602

Strand: Reverse

Name: proB [H]

Synonym: cgR_2239

Alternate gene names: 145296324

Gene position: 2469602-2468382 (Counterclockwise)

Preceding gene: 145296325

Following gene: 145296323

Centisome position: 74.52

GC content: 56.35

Gene sequence:

>1221_bases
GTGACAGGGCTTCCCGTCACCCCCTACAGCCAGGAAGCAAGCATCGGTGCGAGCTTCCCGGCAGTGGATCCGGACACCAA
AGACAGCGCCGCATACGGACATGAATCCGGAATGCGTGAGCGCATCTCCAACGCTAAGCGAGTGGTGGTGAAAATTGGTT
CGTCCTCATTGACTAACGATGAGGATGGACACACCGTCGATCCCAACCGCATCAACACTATTGTCAATGCCTTGCAAGCA
CGCATGGAAGCTGGCTCGGACCTCATCGTTGTGTCCTCTGGCGCAGTGGCCGCGGGAATGGCTCCACTTGGATTGAGCAC
TCGGCCCACGGAATTGGCAGTCAAGCAGGCTGCAGCAGCAGTGGGGCAAGTTCACCTCATGCACCAGTGGGGACGTTCTT
TTGCCCGGTATGGTCGCCCCATCGGCCAGGTGCTTCTTACCGCAGCTGATGCAGGAAAGCGTGATCGTGCGAGGAATGCG
CAGCGTACCATCGACAAGCTGCGCATTTTGGGCGCGGTTCCTATCGTCAATGAAAATGACACCGTGGCAACCACCGGTGT
GAATTTTGGTGACAACGACCGACTTGCTGCAATTGTGGCGCACCTGGTGTCGGCTGATGCTTTGGTGCTGCTCAGTGACG
TGGATGGACTTTTTGATAAGAACCCTACTGATCCCACCGCGAAGTTTATTTCCGAGGTTCGTGACGGCAATGATTTGAAA
GGTGTCATTGCCGGCGACGGCGGAAAAGTGGGCACCGGTGGCATGGCATCAAAGGTGTCTGCTGCACGTTTGGCTTCCCG
AAGTGGCGTGCCTGTGCTGTTGACCTCTGCGGCAAACATTGGCCCAGCACTGGAAGACGCCCAGGTGGGCACTGTATTCC
ACCCCAAGGACAACCGCCTCTCCGCGTGGAAGTTCTGGGCTTTGTATGCCGCAGATACTGCAGGAAAGATCCGACTTGAT
GATGGCGCGGTGGAAGCAGTGACCTCCGGTGGTAAATCTTTGCTGGCTGTGGGCATTACTGAAATCATTGGTGATTTCCA
ACAGGGTGAGATCGTGGAGATCTTGGGACCTGCCGGCCAAATCATCGGGCGAGGCGAGGTGTCCTACGATTCTGATACCT
TGCAATCAATGGTTGGTATGCAAACGCAGGACCTTCCAGATGGCATGCAGCGCCCGGTAGTGCATGCAGATTATCTGTCC
AACTACGCCAGCCGCGCGTAA

Upstream 100 bases:

>100_bases
TCTACGTGTATACGATGGTAGCGCTATGAATGATACGCAGAACACACCTGAAAGCGTTCGATTACGGGATAATCTCCCAA
CGCCAACCCAAATGGCGCCG

Downstream 100 bases:

>100_bases
AGCGCGGGCCTGCTGGTGACGGGTGGCGTCGAAAAGCATTTTTAAAGGAGTTTAAGACGATGAAGTTTGTTATGTATCCG
CATTTGTGGGAGTCCACGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTGLPVTPYSQEASIGASFPAVDPDTKDSAAYGHESGMRERISNAKRVVVKIGSSSLTNDEDGHTVDPNRINTIVNALQA
RMEAGSDLIVVSSGAVAAGMAPLGLSTRPTELAVKQAAAAVGQVHLMHQWGRSFARYGRPIGQVLLTAADAGKRDRARNA
QRTIDKLRILGAVPIVNENDTVATTGVNFGDNDRLAAIVAHLVSADALVLLSDVDGLFDKNPTDPTAKFISEVRDGNDLK
GVIAGDGGKVGTGGMASKVSAARLASRSGVPVLLTSAANIGPALEDAQVGTVFHPKDNRLSAWKFWALYAADTAGKIRLD
DGAVEAVTSGGKSLLAVGITEIIGDFQQGEIVEILGPAGQIIGRGEVSYDSDTLQSMVGMQTQDLPDGMQRPVVHADYLS
NYASRA

Sequences:

>Translated_406_residues
MTGLPVTPYSQEASIGASFPAVDPDTKDSAAYGHESGMRERISNAKRVVVKIGSSSLTNDEDGHTVDPNRINTIVNALQA
RMEAGSDLIVVSSGAVAAGMAPLGLSTRPTELAVKQAAAAVGQVHLMHQWGRSFARYGRPIGQVLLTAADAGKRDRARNA
QRTIDKLRILGAVPIVNENDTVATTGVNFGDNDRLAAIVAHLVSADALVLLSDVDGLFDKNPTDPTAKFISEVRDGNDLK
GVIAGDGGKVGTGGMASKVSAARLASRSGVPVLLTSAANIGPALEDAQVGTVFHPKDNRLSAWKFWALYAADTAGKIRLD
DGAVEAVTSGGKSLLAVGITEIIGDFQQGEIVEILGPAGQIIGRGEVSYDSDTLQSMVGMQTQDLPDGMQRPVVHADYLS
NYASRA
>Mature_405_residues
TGLPVTPYSQEASIGASFPAVDPDTKDSAAYGHESGMRERISNAKRVVVKIGSSSLTNDEDGHTVDPNRINTIVNALQAR
MEAGSDLIVVSSGAVAAGMAPLGLSTRPTELAVKQAAAAVGQVHLMHQWGRSFARYGRPIGQVLLTAADAGKRDRARNAQ
RTIDKLRILGAVPIVNENDTVATTGVNFGDNDRLAAIVAHLVSADALVLLSDVDGLFDKNPTDPTAKFISEVRDGNDLKG
VIAGDGGKVGTGGMASKVSAARLASRSGVPVLLTSAANIGPALEDAQVGTVFHPKDNRLSAWKFWALYAADTAGKIRLDD
GAVEAVTSGGKSLLAVGITEIIGDFQQGEIVEILGPAGQIIGRGEVSYDSDTLQSMVGMQTQDLPDGMQRPVVHADYLSN
YASRA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=303, Percent_Identity=33.6633663366337, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI62912457, Length=301, Percent_Identity=32.890365448505, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786437, Length=351, Percent_Identity=38.7464387464387, Blast_Score=219, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17569967, Length=273, Percent_Identity=34.7985347985348, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=273, Percent_Identity=34.7985347985348, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=406, Percent_Identity=33.4975369458128, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=372, Percent_Identity=33.6021505376344, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21357643, Length=316, Percent_Identity=35.126582278481, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42363; Mature: 42232

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLPVTPYSQEASIGASFPAVDPDTKDSAAYGHESGMRERISNAKRVVVKIGSSSLTND
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCC
EDGHTVDPNRINTIVNALQARMEAGSDLIVVSSGAVAAGMAPLGLSTRPTELAVKQAAAA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHH
VGQVHLMHQWGRSFARYGRPIGQVLLTAADAGKRDRARNAQRTIDKLRILGAVPIVNEND
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCC
TVATTGVNFGDNDRLAAIVAHLVSADALVLLSDVDGLFDKNPTDPTAKFISEVRDGNDLK
CEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCHHHHHHHHHCCCCCCE
GVIAGDGGKVGTGGMASKVSAARLASRSGVPVLLTSAANIGPALEDAQVGTVFHPKDNRL
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCC
SAWKFWALYAADTAGKIRLDDGAVEAVTSGGKSLLAVGITEIIGDFQQGEIVEILGPAGQ
CCEEEEEEEEECCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
IIGRGEVSYDSDTLQSMVGMQTQDLPDGMQRPVVHADYLSNYASRA
EEECCCCCCCHHHHHHHHCCCCCCCCCHHHCCEEEHHHHHHHHCCC
>Mature Secondary Structure 
TGLPVTPYSQEASIGASFPAVDPDTKDSAAYGHESGMRERISNAKRVVVKIGSSSLTND
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCC
EDGHTVDPNRINTIVNALQARMEAGSDLIVVSSGAVAAGMAPLGLSTRPTELAVKQAAAA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHH
VGQVHLMHQWGRSFARYGRPIGQVLLTAADAGKRDRARNAQRTIDKLRILGAVPIVNEND
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCC
TVATTGVNFGDNDRLAAIVAHLVSADALVLLSDVDGLFDKNPTDPTAKFISEVRDGNDLK
CEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCHHHHHHHHHCCCCCCE
GVIAGDGGKVGTGGMASKVSAARLASRSGVPVLLTSAANIGPALEDAQVGTVFHPKDNRL
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCC
SAWKFWALYAADTAGKIRLDDGAVEAVTSGGKSLLAVGITEIIGDFQQGEIVEILGPAGQ
CCEEEEEEEEECCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
IIGRGEVSYDSDTLQSMVGMQTQDLPDGMQRPVVHADYLSNYASRA
EEECCCCCCCHHHHHHHHCCCCCCCCCHHHCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]