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The map label for this gene is aceA

Identifier: 145296297

GI number: 145296297

Start: 2441878

End: 2443176

Strand: Direct

Name: aceA

Synonym: cgR_2212

Alternate gene names: 145296297

Gene position: 2441878-2443176 (Clockwise)

Preceding gene: 145296294

Following gene: 145296298

Centisome position: 73.68

GC content: 58.12

Gene sequence:

>1299_bases
ATGTCAAACGTTGGAAAGCCACGTACCGCACAGGAAATCCAGCAGGATTGGGATACCAACCCTCGTTGGAACGGCATCAC
CCGCGACTACACCGCAGACCAGGTAGCTGATCTGCAGGGTTCCGTCATCGAGGAGCACACTCTTGCTCGCCGCGGCTCAG
AGATCCTCTGGGACGCAGTCACCCAGGAAGGTGACGGATACATCAACGCGCTTGGCGCACTCACCGGTAACCAGGCTGTT
CAGCAGGTTCGTGCAGGCCTGAAGGCTGTCTACCTGTCCGGTTGGCAGGTTGCAGGTGACGCTAACCTCTCCGGCCACAC
CTACCCTGACCAGTCCCTCTACCCAGCGAACTCCGTTCCAAGCGTCGTTCGTCGCATCAACAACGCGCTGCTGCGTTCCG
ATGAAATCGCACGCACCGAAGGCGACACCTCCGTTGACAACTGGGTTGTCCCAATCGTCGCGGACGGCGAAGCTGGCTTC
GGTGGAGCACTCAACGTCTACGAACTCCAGAAGGCAATGATCGCAGCTGGCGCTGCAGGCACCCACTGGGAAGACCAGCT
CGCTTCCGAGAAGAAGTGTGGCCACCTCGGCGGCAAGGTTCTGATCCCAACCCAGCAGCACATCCGCACCCTGAACTCTG
CCCGCCTTGCAGCAGACGTTGCAAACACCCCAACTGTTGTTATCGCACGTACCGACGCTGAGGCAGCAACCCTGATCACC
TCTGACGTTGATGAGCGCGACCAACCATTCATCACCGGTGAGCGCACCGCAGAAGGCTACTACCACGTCAAGAATGGTCT
CGAGCCATGTATCGCACGTGCAAAGTCCTACGCACCATACGCAGATATGATCTGGATGGAGACCGGCACCCCTGACCTGG
AGCTCGCTAAGAAGTTCGCTGAAGGCGTTCGCTCTGAGTTCCCAGACCAGCTGCTGTCCTACAACTGCTCCCCATCCTTC
AACTGGTCTGCACACCTCGAGGCAGATGAGATCGCTAAGTTCCAGAAGGAACTCGGCGCAATGGGCTTCAAGTTCCAGTT
CATCACCCTCGCAGGCTTCCACTCCCTCAACTACGGCATGTTCGACCTGGCTTACGGATACGCTCGCGAAGGCATGACCT
CCTTCGTTGACCTGCAGAACCGTGAGTTCAAGGCAGCTGAAGAGCGTGGCTTCACCGCTGTTAAGCACCAGCGTGAGGTT
GGCGCAGGCTACTTCGACCAGATCGCAACCACCGTTGACCCGAACTCTTCTACCACCGCTTTGAAGGGTTCCACTGAAGA
AGGCCAGTTCCACAACTAG

Upstream 100 bases:

>100_bases
TAAACCGATGCACATCACGTCGAAACAGTGACAGTGCATTAGCTCATACTTTGTGGTTGGCACCGCCCATTGCGAATCAG
CACTTAAGGAAGTGACTTTG

Downstream 100 bases:

>100_bases
GACCTACAGGTTCTGACAATTTAAATCTCCCTACATCTGCACAACGGATGTAGGGAGTTTTTCCTTATATATGCCCTCCA
CAAATCCCCTATCGTGTGAG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSNVGKPRTAQEIQQDWDTNPRWNGITRDYTADQVADLQGSVIEEHTLARRGSEILWDAVTQEGDGYINALGALTGNQAV
QQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVRRINNALLRSDEIARTEGDTSVDNWVVPIVADGEAGF
GGALNVYELQKAMIAAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTVVIARTDAEAATLIT
SDVDERDQPFITGERTAEGYYHVKNGLEPCIARAKSYAPYADMIWMETGTPDLELAKKFAEGVRSEFPDQLLSYNCSPSF
NWSAHLEADEIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTSFVDLQNREFKAAEERGFTAVKHQREV
GAGYFDQIATTVDPNSSTTALKGSTEEGQFHN

Sequences:

>Translated_432_residues
MSNVGKPRTAQEIQQDWDTNPRWNGITRDYTADQVADLQGSVIEEHTLARRGSEILWDAVTQEGDGYINALGALTGNQAV
QQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVRRINNALLRSDEIARTEGDTSVDNWVVPIVADGEAGF
GGALNVYELQKAMIAAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTVVIARTDAEAATLIT
SDVDERDQPFITGERTAEGYYHVKNGLEPCIARAKSYAPYADMIWMETGTPDLELAKKFAEGVRSEFPDQLLSYNCSPSF
NWSAHLEADEIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTSFVDLQNREFKAAEERGFTAVKHQREV
GAGYFDQIATTVDPNSSTTALKGSTEEGQFHN
>Mature_431_residues
SNVGKPRTAQEIQQDWDTNPRWNGITRDYTADQVADLQGSVIEEHTLARRGSEILWDAVTQEGDGYINALGALTGNQAVQ
QVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVRRINNALLRSDEIARTEGDTSVDNWVVPIVADGEAGFG
GALNVYELQKAMIAAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTVVIARTDAEAATLITS
DVDERDQPFITGERTAEGYYHVKNGLEPCIARAKSYAPYADMIWMETGTPDLELAKKFAEGVRSEFPDQLLSYNCSPSFN
WSAHLEADEIAKFQKELGAMGFKFQFITLAGFHSLNYGMFDLAYGYAREGMTSFVDLQNREFKAAEERGFTAVKHQREVG
AGYFDQIATTVDPNSSTTALKGSTEEGQFHN

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=58.3908045977011, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1786525, Length=276, Percent_Identity=28.9855072463768, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982926, Length=416, Percent_Identity=59.8557692307692, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17561814, Length=416, Percent_Identity=59.8557692307692, Blast_Score=506, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6320908, Length=260, Percent_Identity=36.5384615384615, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325263, Length=253, Percent_Identity=35.5731225296443, Blast_Score=133, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEA_CORGL (P42449)

Other databases:

- EMBL:   X75504
- EMBL:   BA000036
- EMBL:   BX927154
- PIR:   I40713
- RefSeq:   NP_601531.1
- RefSeq:   YP_226575.1
- ProteinModelPortal:   P42449
- SMR:   P42449
- DIP:   DIP-2898N
- GeneID:   1020281
- GeneID:   3345421
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2560
- KEGG:   cgl:NCgl2248
- HOGENOM:   HBG308645
- OMA:   QAVQQVK
- PhylomeDB:   P42449
- ProtClustDB:   PRK15063
- BioCyc:   CGLU196627:CG2560-MONOMER
- BRENDA:   4.1.3.1
- GO:   GO:0005737
- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR21631:SF3
- PIRSF:   PIRSF001362
- TIGRFAMs:   TIGR01346

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.1

Molecular weight: Translated: 47229; Mature: 47098

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVGKPRTAQEIQQDWDTNPRWNGITRDYTADQVADLQGSVIEEHTLARRGSEILWDAV
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
TQEGDGYINALGALTGNQAVQQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVP
HCCCCCCEEHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHH
SVVRRINNALLRSDEIARTEGDTSVDNWVVPIVADGEAGFGGALNVYELQKAMIAAGAAG
HHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
THWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTVVIARTDAEAATLIT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHEEEE
SDVDERDQPFITGERTAEGYYHVKNGLEPCIARAKSYAPYADMIWMETGTPDLELAKKFA
CCCCCCCCCEECCCCCCCCCHHHHCCHHHHHHHHHCCCCCHHEEEEECCCCHHHHHHHHH
EGVRSEFPDQLLSYNCSPSFNWSAHLEADEIAKFQKELGAMGFKFQFITLAGFHSLNYGM
HHHHHHCCHHHHCCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEEEECHHHCCCCH
FDLAYGYAREGMTSFVDLQNREFKAAEERGFTAVKHQREVGAGYFDQIATTVDPNSSTTA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEE
LKGSTEEGQFHN
ECCCCCCCCCCC
>Mature Secondary Structure 
SNVGKPRTAQEIQQDWDTNPRWNGITRDYTADQVADLQGSVIEEHTLARRGSEILWDAV
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
TQEGDGYINALGALTGNQAVQQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVP
HCCCCCCEEHHHHHCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHH
SVVRRINNALLRSDEIARTEGDTSVDNWVVPIVADGEAGFGGALNVYELQKAMIAAGAAG
HHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
THWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTVVIARTDAEAATLIT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHEEEE
SDVDERDQPFITGERTAEGYYHVKNGLEPCIARAKSYAPYADMIWMETGTPDLELAKKFA
CCCCCCCCCEECCCCCCCCCHHHHCCHHHHHHHHHCCCCCHHEEEEECCCCHHHHHHHHH
EGVRSEFPDQLLSYNCSPSFNWSAHLEADEIAKFQKELGAMGFKFQFITLAGFHSLNYGM
HHHHHHCCHHHHCCCCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEEEEEECHHHCCCCH
FDLAYGYAREGMTSFVDLQNREFKAAEERGFTAVKHQREVGAGYFDQIATTVDPNSSTTA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCEE
LKGSTEEGQFHN
ECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8206824; 12948626