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The map label for this gene is glcB [H]

Identifier: 145296295

GI number: 145296295

Start: 2439060

End: 2441279

Strand: Reverse

Name: glcB [H]

Synonym: cgR_2211

Alternate gene names: 145296295

Gene position: 2441279-2439060 (Counterclockwise)

Preceding gene: 145296296

Following gene: 145296293

Centisome position: 73.66

GC content: 55.54

Gene sequence:

>2220_bases
ATGACTGAACAGGAACTGTTGTCTGCTCAGACTGCCGACAACGCTGGAACTGACAGCACCGAACGCGTTGACGCGGGCGG
AATGCAGGTTGCAAAAGTTCTCTACGACTTTGTAACCGAAGCGGTACTCCCTCGCGTGGGTGTGGATGCGGAAAAGTTCT
GGTCCGGATTCGCCGCCATCGCCCGGGACCTCACCCCACGCAACCGCGAACTGCTTGCTCGTCGCGATGAACTGCAGACG
CTTATCGACGACTACCACCGCAACAACTCCGGCACCATCGACCAAGACGCGTACGAGGATTTCCTTAAAGAAATCGGATA
CTTGGTTGAGGAGCCAGAAGCTGCAGAAATCCGTACCCAAAACGTCGATACGGAAATCTCCAGCACCGCAGGACCTCAGC
TGGTTGTGCCAATTCTGAACGCACGCTTCGCGCTGAATGCTGCCAATGCTCGTTGGGGTTCCCTCTACGATGCGTTGTAC
GGCACCAACGCCATCCCAGAAACTGATGGCGCTGAAAAGGGCAAGGAGTACAACCCGGTCCGCGGCCAGAAGGTCATCGA
GTGGGGTCGTCAATTCCTCGACAGCGTTGTCCCACTGGACGGTGCTTCGCATGCCGATGTTGAGAAGTACAACATCACGG
ATGGAAAGCTTGCAGCCCATGTTGGCGACAGCGTCTACCGTTTGAAGAATCGCGATTCTTACCGCGGCTTCACCGGCAAC
TTCCTTGATCCAGAAGTAATCCTGCTGGAAACCAATGGCCTGCACATTGAGCTTCAGATCGATCCTGTCCACCCAATCGG
CAAGGCAGACAAGACCGGTCTGAAAGACATCGTTTTGGAATCTGCGATCACCACGATCATGGACTTCGAAGACTCCGTTG
CAGCTGTTGATGCTGAAGACAAGACCTTAGGTTACTCTAACTGGTTCGGACTCAACACCGGCGAACTGAAAGAAGAGATG
TCCAAGAACGGACGCACCTTCACCCGTGAGCTCAATAAGGACCGCGTCTACATTGGCCGCAATGGTACCGAGCTGGTTCT
GCACGGTCGTTCCCTGCTGTTCGTCCGCAACGTTGGTCACCTCATGCGAAACCCATCCATCTTGATTGATGGCGAGGAGA
TCTTCGAAGGCATCATGGATGCTGTTCTCACCACTGTTTGTGCGATTCCAGGAATTGCTCCGCAGAACAAGATGCGCAAT
TCCCGCAAGGGCTCCATCTACATCGTGAAGCCTAAACAGCACGGCCCTGAAGAAGTCGCGTTCACCAACGAACTCTTCGG
CCGCGTTGAGGATCTGCTCGATCTGCCACGCCATACCCTCAAGGTTGGTGTCATGGATGAAGAGCGTCGCACCTCCGTCA
ACCTGGATGCCTGCATCATGGAAGTTGCTGACCGCCTGGCGTTCATTAACACCGGCTTCCTGGATCGCACCGGCGATGAG
ATTCACACCTCCATGGAGGCAGGCGCAATGGTGCGCAAGGCTGACATGCAGACTGCACCGTGGAAGCAGGCCTACGAGAA
CAACAACGTTGATGCAGGTATTCAGCGTGGTCTTCCTGGCAAGGCTCAGATCGGTAAGGGCATGTGGGCGATGACTGAAC
TCATGGCAGAAATGCTGGAGAAGAAGATCGGCCAGCCACGCGAAGGCGCCAACACTGCATGGGTTCCTTCACCAACTGGT
GCGACGCTGCACGCAACGCACTACCACTTGGTTGATGTGTTCAAGGTTCAAGACGAACTGCGTGCTGCCGGCCGCCGCGA
CAGCCTGCGCAACATTCTCACCATTCCAACCGCACCAAACACCAATTGGTCTGAGGAAGAGAAGAAGGAAGAGATGGACA
ACAACTGCCAGTCCATCCTCGGATACGTTGTGCGCTGGGTTGAACACGGTGTTGGTTGCTCCAAGGTTCCAGACATCCAT
GACATCGACCTCATGGAAGACCGCGCAACGCTGCGTATTTCCTCGCAGATGCTGGCCAACTGGATCCGCCATGATGTTGT
CTCGAAGGAGCAGGTCTTGGAGTCACTGGAACGAATGGCAGTGGTCGTCGACAAGCAAAATGCGGGCGACGAGGCCTACC
GCGATATGGCGCCGAACTACGACGCCTCCCTCGCCTTCCAGGCGGCTAAGGACTTGATTTTCGAAGGCACCAAGTCCCCA
TCGGGCTACACCGAGCCCATCTTGCACGCACGCCGCCGCGAGTTCAAAGCAAAAAACTAA

Upstream 100 bases:

>100_bases
GTTTTCACAACCGTTAACGGCGTAGCCAAACAAGAAGGATTCGCATTCTTCTGGTTTAGGCACAGGTCATCTAAAACCCA
TGCTTTAAAAGGAGCCTTCA

Downstream 100 bases:

>100_bases
GCACGCTTTTCGACGCTTACCTGCATCCCAACGGTGACTGACTGCCCCGGAGCCACCCTCACTCCTTTTTGGTCAGCACC
CAAAAGCGCCGGTTCAACAC

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTEQELLSAQTADNAGTDSTERVDAGGMQVAKVLYDFVTEAVLPRVGVDAEKFWSGFAAIARDLTPRNRELLARRDELQT
LIDDYHRNNSGTIDQDAYEDFLKEIGYLVEEPEAAEIRTQNVDTEISSTAGPQLVVPILNARFALNAANARWGSLYDALY
GTNAIPETDGAEKGKEYNPVRGQKVIEWGRQFLDSVVPLDGASHADVEKYNITDGKLAAHVGDSVYRLKNRDSYRGFTGN
FLDPEVILLETNGLHIELQIDPVHPIGKADKTGLKDIVLESAITTIMDFEDSVAAVDAEDKTLGYSNWFGLNTGELKEEM
SKNGRTFTRELNKDRVYIGRNGTELVLHGRSLLFVRNVGHLMRNPSILIDGEEIFEGIMDAVLTTVCAIPGIAPQNKMRN
SRKGSIYIVKPKQHGPEEVAFTNELFGRVEDLLDLPRHTLKVGVMDEERRTSVNLDACIMEVADRLAFINTGFLDRTGDE
IHTSMEAGAMVRKADMQTAPWKQAYENNNVDAGIQRGLPGKAQIGKGMWAMTELMAEMLEKKIGQPREGANTAWVPSPTG
ATLHATHYHLVDVFKVQDELRAAGRRDSLRNILTIPTAPNTNWSEEEKKEEMDNNCQSILGYVVRWVEHGVGCSKVPDIH
DIDLMEDRATLRISSQMLANWIRHDVVSKEQVLESLERMAVVVDKQNAGDEAYRDMAPNYDASLAFQAAKDLIFEGTKSP
SGYTEPILHARRREFKAKN

Sequences:

>Translated_739_residues
MTEQELLSAQTADNAGTDSTERVDAGGMQVAKVLYDFVTEAVLPRVGVDAEKFWSGFAAIARDLTPRNRELLARRDELQT
LIDDYHRNNSGTIDQDAYEDFLKEIGYLVEEPEAAEIRTQNVDTEISSTAGPQLVVPILNARFALNAANARWGSLYDALY
GTNAIPETDGAEKGKEYNPVRGQKVIEWGRQFLDSVVPLDGASHADVEKYNITDGKLAAHVGDSVYRLKNRDSYRGFTGN
FLDPEVILLETNGLHIELQIDPVHPIGKADKTGLKDIVLESAITTIMDFEDSVAAVDAEDKTLGYSNWFGLNTGELKEEM
SKNGRTFTRELNKDRVYIGRNGTELVLHGRSLLFVRNVGHLMRNPSILIDGEEIFEGIMDAVLTTVCAIPGIAPQNKMRN
SRKGSIYIVKPKQHGPEEVAFTNELFGRVEDLLDLPRHTLKVGVMDEERRTSVNLDACIMEVADRLAFINTGFLDRTGDE
IHTSMEAGAMVRKADMQTAPWKQAYENNNVDAGIQRGLPGKAQIGKGMWAMTELMAEMLEKKIGQPREGANTAWVPSPTG
ATLHATHYHLVDVFKVQDELRAAGRRDSLRNILTIPTAPNTNWSEEEKKEEMDNNCQSILGYVVRWVEHGVGCSKVPDIH
DIDLMEDRATLRISSQMLANWIRHDVVSKEQVLESLERMAVVVDKQNAGDEAYRDMAPNYDASLAFQAAKDLIFEGTKSP
SGYTEPILHARRREFKAKN
>Mature_738_residues
TEQELLSAQTADNAGTDSTERVDAGGMQVAKVLYDFVTEAVLPRVGVDAEKFWSGFAAIARDLTPRNRELLARRDELQTL
IDDYHRNNSGTIDQDAYEDFLKEIGYLVEEPEAAEIRTQNVDTEISSTAGPQLVVPILNARFALNAANARWGSLYDALYG
TNAIPETDGAEKGKEYNPVRGQKVIEWGRQFLDSVVPLDGASHADVEKYNITDGKLAAHVGDSVYRLKNRDSYRGFTGNF
LDPEVILLETNGLHIELQIDPVHPIGKADKTGLKDIVLESAITTIMDFEDSVAAVDAEDKTLGYSNWFGLNTGELKEEMS
KNGRTFTRELNKDRVYIGRNGTELVLHGRSLLFVRNVGHLMRNPSILIDGEEIFEGIMDAVLTTVCAIPGIAPQNKMRNS
RKGSIYIVKPKQHGPEEVAFTNELFGRVEDLLDLPRHTLKVGVMDEERRTSVNLDACIMEVADRLAFINTGFLDRTGDEI
HTSMEAGAMVRKADMQTAPWKQAYENNNVDAGIQRGLPGKAQIGKGMWAMTELMAEMLEKKIGQPREGANTAWVPSPTGA
TLHATHYHLVDVFKVQDELRAAGRRDSLRNILTIPTAPNTNWSEEEKKEEMDNNCQSILGYVVRWVEHGVGCSKVPDIHD
IDLMEDRATLRISSQMLANWIRHDVVSKEQVLESLERMAVVVDKQNAGDEAYRDMAPNYDASLAFQAAKDLIFEGTKSPS
GYTEPILHARRREFKAKN

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=723, Percent_Identity=56.0165975103734, Blast_Score=805, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 82351; Mature: 82219

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50222 EF_HAND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQELLSAQTADNAGTDSTERVDAGGMQVAKVLYDFVTEAVLPRVGVDAEKFWSGFAAI
CCHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ARDLTPRNRELLARRDELQTLIDDYHRNNSGTIDQDAYEDFLKEIGYLVEEPEAAEIRTQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHC
NVDTEISSTAGPQLVVPILNARFALNAANARWGSLYDALYGTNAIPETDGAEKGKEYNPV
CCCCHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RGQKVIEWGRQFLDSVVPLDGASHADVEKYNITDGKLAAHVGDSVYRLKNRDSYRGFTGN
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHCCHHHHHCCCCCCCCCCCC
FLDPEVILLETNGLHIELQIDPVHPIGKADKTGLKDIVLESAITTIMDFEDSVAAVDAED
CCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCC
KTLGYSNWFGLNTGELKEEMSKNGRTFTRELNKDRVYIGRNGTELVLHGRSLLFVRNVGH
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCEEEEECCEEEEHHHHHH
LMRNPSILIDGEEIFEGIMDAVLTTVCAIPGIAPQNKMRNSRKGSIYIVKPKQHGPEEVA
HHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCCHHHH
FTNELFGRVEDLLDLPRHTLKVGVMDEERRTSVNLDACIMEVADRLAFINTGFLDRTGDE
HHHHHHHHHHHHHHCCHHHEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
IHTSMEAGAMVRKADMQTAPWKQAYENNNVDAGIQRGLPGKAQIGKGMWAMTELMAEMLE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHCCCCCHHHHCCHHHHHHHHHHHHHH
KKIGQPREGANTAWVPSPTGATLHATHYHLVDVFKVQDELRAAGRRDSLRNILTIPTAPN
HHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHCCEEECCCCCC
TNWSEEEKKEEMDNNCQSILGYVVRWVEHGVGCSKVPDIHDIDLMEDRATLRISSQMLAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
WIRHDVVSKEQVLESLERMAVVVDKQNAGDEAYRDMAPNYDASLAFQAAKDLIFEGTKSP
HHHHHHCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
SGYTEPILHARRREFKAKN
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEQELLSAQTADNAGTDSTERVDAGGMQVAKVLYDFVTEAVLPRVGVDAEKFWSGFAAI
CHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ARDLTPRNRELLARRDELQTLIDDYHRNNSGTIDQDAYEDFLKEIGYLVEEPEAAEIRTQ
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHC
NVDTEISSTAGPQLVVPILNARFALNAANARWGSLYDALYGTNAIPETDGAEKGKEYNPV
CCCCHHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RGQKVIEWGRQFLDSVVPLDGASHADVEKYNITDGKLAAHVGDSVYRLKNRDSYRGFTGN
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHHCCHHHHHCCCCCCCCCCCC
FLDPEVILLETNGLHIELQIDPVHPIGKADKTGLKDIVLESAITTIMDFEDSVAAVDAED
CCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCC
KTLGYSNWFGLNTGELKEEMSKNGRTFTRELNKDRVYIGRNGTELVLHGRSLLFVRNVGH
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCEEEEECCEEEEHHHHHH
LMRNPSILIDGEEIFEGIMDAVLTTVCAIPGIAPQNKMRNSRKGSIYIVKPKQHGPEEVA
HHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECCCCCCHHHH
FTNELFGRVEDLLDLPRHTLKVGVMDEERRTSVNLDACIMEVADRLAFINTGFLDRTGDE
HHHHHHHHHHHHHHCCHHHEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
IHTSMEAGAMVRKADMQTAPWKQAYENNNVDAGIQRGLPGKAQIGKGMWAMTELMAEMLE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHCCCCCHHHHCCHHHHHHHHHHHHHH
KKIGQPREGANTAWVPSPTGATLHATHYHLVDVFKVQDELRAAGRRDSLRNILTIPTAPN
HHCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHCCEEECCCCCC
TNWSEEEKKEEMDNNCQSILGYVVRWVEHGVGCSKVPDIHDIDLMEDRATLRISSQMLAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
WIRHDVVSKEQVLESLERMAVVVDKQNAGDEAYRDMAPNYDASLAFQAAKDLIFEGTKSP
HHHHHHCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
SGYTEPILHARRREFKAKN
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7812449; 12948626 [H]