Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 145296285

GI number: 145296285

Start: 2429000

End: 2430511

Strand: Direct

Name: gsiB [H]

Synonym: cgR_2201

Alternate gene names: 145296285

Gene position: 2429000-2430511 (Clockwise)

Preceding gene: 145296282

Following gene: 145296286

Centisome position: 73.29

GC content: 56.15

Gene sequence:

>1512_bases
ATGAAGATCACGCGCGGACTCCTGCCATCATTGCTGTTGGCAAGCACAATCGTGGTGTCGTCATGCTCTGCTGGATCGAC
TGCGTATCAGCAGCCCCCTGCTGTTGATCAATCATCCATTGTCATTGCTACCACGGCTGCTGCGGCGTCACTTGATTTCA
CCAATGCTGCGGGCGCTGCTATTCCGCAGGCCATGATGTCCAATATTTACGAGGGGCTTGTGCGCATCGATGCGGAGGGT
GAGATTCAGCCGCTGCTTGCCAAGTCGTGGGATATTTCAGACGATCGCACCGAGTACATTTTCCATTTGCGGGAGGGTGT
GCTGTTTTCCAACGGCGATCCCTTCAATGCTGATTCCGCGAAGTTTTCCATTGATCGGGTAAAAACTGACTGGACCAATG
GTTTGAAAAGTGGCATGGATGTGGTGGAGTCCACCGAGGTGATTGACGATCACACGCTGAAAGTTTCGCTGGTTAGGCCG
TCCAACCAATGGTTGTGGAGCATGGGTACCGCGATCGGTGCCATGATGACGGAGGGGGGCGTCGATAAGCTGGCAACTGA
TCCCGTTGGCACCGGCCCGTACACGGTGACGCACTGGGCGCCGGGCCGCGCAATTGGGTTCGGCGCGCGGGCCGATTATT
GGGGGCAGAAGCCGCTTAACGACGCCGCAACCATCCGTTACTTCAGCGATGCGACGGCCTCGACCAATGCGCTGCAAAGC
GGTGACGTGGACGTGATTTGGGCGATGCAAGCGCCCGAACAGCTGGCTACGCTGCAGGAATACACCGTGGAAGTGGGCAC
AACCAATGGTGAGATGTTGCTGTCGATGAATAATCAGCGTGCACCTTTTGATGATGTGCGTGTGCGCCAGGCGGTGATGT
TTGCGATTGATCGCCAAGCCGTCATTGATACCGCCTTAGAAGGCTACGGCACCGACACTGGTGGCGCGCCTGTTCCGCCG
ACTGATCCGTGGTACGAGAAATCCACGATGTACCCCTACGATCCGGACCGCGCACGGGCATTGTTAGAGGAAGCCGGCGC
TGAGGGAACGCGGATCACCATGTCCATTCCTTCGTTGCCGTACGCTCAGGCAGCCTCTGAAATCCTGTACTCGCAACTGC
GAGATGTTGGTTTTGATCCTGTGATTGAATCAACCGAGTTCCCAGCCGTCTGGTTGGCACAAGTCATGGGGCAAAAAGAC
TACGACATGTCACTAATCGCGCATGTGGAACCCCGCGACATCCCCACGCTGTTTAGCCCCAACTACTATTTGGGCTTTGA
CGACGCCGAAACCCAAGCCCTCCTCGCAGAGGCAGACAGTTCAGCAAACGAAGTGGAATTGATGCAACAAGCTGTCGATC
GAATCATGGAACAAGCCGTCGCCGACAACCTCATGAACGTGGCCAACATCGTGGTGATGTCACCAGAGATCACCGGCATT
GATCCCAACGTGGTGTCCGGGGCGTTGGAATTGTCGTTGATTGGTCGGAAAGAATCCGGGGTAGCGCAGTGA

Upstream 100 bases:

>100_bases
GGATTTTCCATTGGCGGAGGTTCGTGCGGCGGGTATCCATTGCCTTTCATTTTAGTTTTCCATTTAGTTTCCCGGATCAC
ACCGACTAATCTCAGAAGCC

Downstream 100 bases:

>100_bases
GTAAAACAATCGCTTGGACTGTGCTACGGTACACCCTGACTTTTGTGATAGCCAGCATCATCATTTTTGTGCTGATTCGA
GTCATCCCCGGTGACCCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKITRGLLPSLLLASTIVVSSCSAGSTAYQQPPAVDQSSIVIATTAAAASLDFTNAAGAAIPQAMMSNIYEGLVRIDAEG
EIQPLLAKSWDISDDRTEYIFHLREGVLFSNGDPFNADSAKFSIDRVKTDWTNGLKSGMDVVESTEVIDDHTLKVSLVRP
SNQWLWSMGTAIGAMMTEGGVDKLATDPVGTGPYTVTHWAPGRAIGFGARADYWGQKPLNDAATIRYFSDATASTNALQS
GDVDVIWAMQAPEQLATLQEYTVEVGTTNGEMLLSMNNQRAPFDDVRVRQAVMFAIDRQAVIDTALEGYGTDTGGAPVPP
TDPWYEKSTMYPYDPDRARALLEEAGAEGTRITMSIPSLPYAQAASEILYSQLRDVGFDPVIESTEFPAVWLAQVMGQKD
YDMSLIAHVEPRDIPTLFSPNYYLGFDDAETQALLAEADSSANEVELMQQAVDRIMEQAVADNLMNVANIVVMSPEITGI
DPNVVSGALELSLIGRKESGVAQ

Sequences:

>Translated_503_residues
MKITRGLLPSLLLASTIVVSSCSAGSTAYQQPPAVDQSSIVIATTAAAASLDFTNAAGAAIPQAMMSNIYEGLVRIDAEG
EIQPLLAKSWDISDDRTEYIFHLREGVLFSNGDPFNADSAKFSIDRVKTDWTNGLKSGMDVVESTEVIDDHTLKVSLVRP
SNQWLWSMGTAIGAMMTEGGVDKLATDPVGTGPYTVTHWAPGRAIGFGARADYWGQKPLNDAATIRYFSDATASTNALQS
GDVDVIWAMQAPEQLATLQEYTVEVGTTNGEMLLSMNNQRAPFDDVRVRQAVMFAIDRQAVIDTALEGYGTDTGGAPVPP
TDPWYEKSTMYPYDPDRARALLEEAGAEGTRITMSIPSLPYAQAASEILYSQLRDVGFDPVIESTEFPAVWLAQVMGQKD
YDMSLIAHVEPRDIPTLFSPNYYLGFDDAETQALLAEADSSANEVELMQQAVDRIMEQAVADNLMNVANIVVMSPEITGI
DPNVVSGALELSLIGRKESGVAQ
>Mature_503_residues
MKITRGLLPSLLLASTIVVSSCSAGSTAYQQPPAVDQSSIVIATTAAAASLDFTNAAGAAIPQAMMSNIYEGLVRIDAEG
EIQPLLAKSWDISDDRTEYIFHLREGVLFSNGDPFNADSAKFSIDRVKTDWTNGLKSGMDVVESTEVIDDHTLKVSLVRP
SNQWLWSMGTAIGAMMTEGGVDKLATDPVGTGPYTVTHWAPGRAIGFGARADYWGQKPLNDAATIRYFSDATASTNALQS
GDVDVIWAMQAPEQLATLQEYTVEVGTTNGEMLLSMNNQRAPFDDVRVRQAVMFAIDRQAVIDTALEGYGTDTGGAPVPP
TDPWYEKSTMYPYDPDRARALLEEAGAEGTRITMSIPSLPYAQAASEILYSQLRDVGFDPVIESTEFPAVWLAQVMGQKD
YDMSLIAHVEPRDIPTLFSPNYYLGFDDAETQALLAEADSSANEVELMQQAVDRIMEQAVADNLMNVANIVVMSPEITGI
DPNVVSGALELSLIGRKESGVAQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=490, Percent_Identity=28.9795918367347, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1789966, Length=475, Percent_Identity=28.8421052631579, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787551, Length=386, Percent_Identity=26.4248704663212, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789887, Length=470, Percent_Identity=27.0212765957447, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1787762, Length=450, Percent_Identity=23.3333333333333, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787495, Length=383, Percent_Identity=24.0208877284595, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789397, Length=267, Percent_Identity=25.8426966292135, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082063, Length=281, Percent_Identity=26.6903914590747, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081878, Length=286, Percent_Identity=24.8251748251748, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54306; Mature: 54306

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRGLLPSLLLASTIVVSSCSAGSTAYQQPPAVDQSSIVIATTAAAASLDFTNAAGAA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCCCHH
IPQAMMSNIYEGLVRIDAEGEIQPLLAKSWDISDDRTEYIFHLREGVLFSNGDPFNADSA
HHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCC
KFSIDRVKTDWTNGLKSGMDVVESTEVIDDHTLKVSLVRPSNQWLWSMGTAIGAMMTEGG
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
VDKLATDPVGTGPYTVTHWAPGRAIGFGARADYWGQKPLNDAATIRYFSDATASTNALQS
CHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
GDVDVIWAMQAPEQLATLQEYTVEVGTTNGEMLLSMNNQRAPFDDVRVRQAVMFAIDRQA
CCEEEEEEECCCHHHHHHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VIDTALEGYGTDTGGAPVPPTDPWYEKSTMYPYDPDRARALLEEAGAEGTRITMSIPSLP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
YAQAASEILYSQLRDVGFDPVIESTEFPAVWLAQVMGQKDYDMSLIAHVEPRDIPTLFSP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEECC
NYYLGFDDAETQALLAEADSSANEVELMQQAVDRIMEQAVADNLMNVANIVVMSPEITGI
CEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
DPNVVSGALELSLIGRKESGVAQ
CHHHHHCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKITRGLLPSLLLASTIVVSSCSAGSTAYQQPPAVDQSSIVIATTAAAASLDFTNAAGAA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCCCHH
IPQAMMSNIYEGLVRIDAEGEIQPLLAKSWDISDDRTEYIFHLREGVLFSNGDPFNADSA
HHHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCC
KFSIDRVKTDWTNGLKSGMDVVESTEVIDDHTLKVSLVRPSNQWLWSMGTAIGAMMTEGG
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCC
VDKLATDPVGTGPYTVTHWAPGRAIGFGARADYWGQKPLNDAATIRYFSDATASTNALQS
CHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
GDVDVIWAMQAPEQLATLQEYTVEVGTTNGEMLLSMNNQRAPFDDVRVRQAVMFAIDRQA
CCEEEEEEECCCHHHHHHHHHEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VIDTALEGYGTDTGGAPVPPTDPWYEKSTMYPYDPDRARALLEEAGAEGTRITMSIPSLP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCC
YAQAASEILYSQLRDVGFDPVIESTEFPAVWLAQVMGQKDYDMSLIAHVEPRDIPTLFSP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEECC
NYYLGFDDAETQALLAEADSSANEVELMQQAVDRIMEQAVADNLMNVANIVVMSPEITGI
CEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
DPNVVSGALELSLIGRKESGVAQ
CHHHHHCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA