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The map label for this gene is opuD [H]

Identifier: 145296267

GI number: 145296267

Start: 2410021

End: 2411676

Strand: Reverse

Name: opuD [H]

Synonym: cgR_2184

Alternate gene names: 145296267

Gene position: 2411676-2410021 (Counterclockwise)

Preceding gene: 145296268

Following gene: 145296263

Centisome position: 72.77

GC content: 48.67

Gene sequence:

>1656_bases
ATGTATATCGGGGGTGTCTCCTTGGTTTTCATTTTCCTCATGGGTATCTTTGTGTCCCGGTATGGCCGGGTAAAACTTGG
TGATGATGATGATGACCCCGAGCACACCCTAATCGTGTGGTTCTGTATGCTTTTTGCTGGCGGTGTCGGTGCAGTCTTAA
TGTTTTGGGGTGTTGCCGAACCGATTAACCACGCGTTCAACGTGCCAATGGCCAATGAAGAATCCATGAGTGAAGCCGCA
ATTGTGCAGGCTTTTGCTTATACTTTCTATCACTTCGGTATTCACATGTGGGTAATCATGGCACTCCCAGGATTATCATT
GGGATACTTTATTTACAAACGTAAGCTACCTCCGCGTCTATCCTCTGTGTTTTCTCCGATCTTGGGTAAGCACATTTATT
CCACACCCGGCAAGCTCATCGATGTACTGGCCATCGTAGGCACCACGTTTGGTATTGCTGTGTCAGTAGGTCTTGGTGTG
CTGCAAATCAATGCAGGTATGAACAAACTTTGGAGCACCCCTCAAGTATCGTGGGTTCAGCTTTTGATCATCTTGATCAT
CACCGCGGTTGCATGTATTTCCGTTGCTTCCGGTTTGGATAAGGGCATTAAGTTACTGTCCAACATTAATATTGCAATGG
CCGTTGCGTTGATGTTCTTCATCTTGTTCACTGGTCCAACCCTCACATTGCTGCGCTTTCTCGTAGAATCCTTCGGAATC
TACGCATCCTGGATGCCTAATCTGATGTTTTGGACTGACTCTTTCCAAGATAACCCAGGCTGGCAGGGCAAATGGACGGT
GTTCTATTGGGCATGGACTATTTGTTGGTCGCCATATGTCGGCATGTTCGTGGCGCGTATTTCGCGTGGACGTACCGTCC
GTGAATTTATCGGTGGGGTTCTAGCTCTGCCAGCGATCTTTGGCGTAGTTTGGTTCTCTATCTTTGGTCGTGCAGGCATC
GAAGTGGAACTGAGTAACCCAGGTTTCTTGACCCAGCCAACTGTTGTTGAAGGTGACGTGCCAGCAGCGCTTTTTAATGT
GCTGCAAGAGTATCCGCTGACTGGAATTGTCTCCGCGTTTGCACTTGTAATTATTGTGATTTTCTTTATCACCTCCATCG
ATTCCGCAGCGCTAGTTAACGATATGTTCGCTACCGGTGAAGAAAATCAAACACCGACTAGTTACCGCGTGATGTGGGCC
TGCACCATTGGGGCGGTCGCAGGTTCCTTGCTGATCATTTCCCCATCCTCTGGTATTGCCACGCTGCAAGAAGTGGTTAT
CATCGTGGCTTTCCCATTCTTCCTCGTGCAATTTGTCATGATGTTTTCTTTGCTTAAAGGCATGAGTGAAGATGCTGCTG
CGGTTCGTCGTGTGCAGACTCGTCAGTGGGAAAAGACTGATACACCAGAAAAACTTGAAGAGCATTCGTCCCAACCAGCC
CCGGGCTATGATGACGAGGGCAACCCCTTGCCGATGCCTGCCCTCGAACATGATGAGGACGGTAACATTGTTATCCCAGG
CAACGTAGTCATTGAAGGTGATCTTGGGGTAGTTGGTGATGTGGTCGACGATCCTGAGGAAGCCCAAGAGATGGGCTCTC
GTTTTAAGATCGTCGAGCAAACTCGCCCCCAGTCCAGGGACGAATACGATATTTAA

Upstream 100 bases:

>100_bases
TTTTATCGTGGTGTTTGTCATCGCCACAATTGCGCTAGGCGAGAAAGCTCGAACAACCTTTTCCGCGGTTGCCGGCTGGC
TCTTAGAAAATTTAGGGTGG

Downstream 100 bases:

>100_bases
ACGATTGCTTTTCGACGCACCTCCCACCCTGTTTAAGTATCCCGCGTGGGGTGGGACGGCAGAGAATCGTTGGTAAAAAC
CCAGCCTTGTGGAAAACCTG

Product: hypothetical protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MYIGGVSLVFIFLMGIFVSRYGRVKLGDDDDDPEHTLIVWFCMLFAGGVGAVLMFWGVAEPINHAFNVPMANEESMSEAA
IVQAFAYTFYHFGIHMWVIMALPGLSLGYFIYKRKLPPRLSSVFSPILGKHIYSTPGKLIDVLAIVGTTFGIAVSVGLGV
LQINAGMNKLWSTPQVSWVQLLIILIITAVACISVASGLDKGIKLLSNINIAMAVALMFFILFTGPTLTLLRFLVESFGI
YASWMPNLMFWTDSFQDNPGWQGKWTVFYWAWTICWSPYVGMFVARISRGRTVREFIGGVLALPAIFGVVWFSIFGRAGI
EVELSNPGFLTQPTVVEGDVPAALFNVLQEYPLTGIVSAFALVIIVIFFITSIDSAALVNDMFATGEENQTPTSYRVMWA
CTIGAVAGSLLIISPSSGIATLQEVVIIVAFPFFLVQFVMMFSLLKGMSEDAAAVRRVQTRQWEKTDTPEKLEEHSSQPA
PGYDDEGNPLPMPALEHDEDGNIVIPGNVVIEGDLGVVGDVVDDPEEAQEMGSRFKIVEQTRPQSRDEYDI

Sequences:

>Translated_551_residues
MYIGGVSLVFIFLMGIFVSRYGRVKLGDDDDDPEHTLIVWFCMLFAGGVGAVLMFWGVAEPINHAFNVPMANEESMSEAA
IVQAFAYTFYHFGIHMWVIMALPGLSLGYFIYKRKLPPRLSSVFSPILGKHIYSTPGKLIDVLAIVGTTFGIAVSVGLGV
LQINAGMNKLWSTPQVSWVQLLIILIITAVACISVASGLDKGIKLLSNINIAMAVALMFFILFTGPTLTLLRFLVESFGI
YASWMPNLMFWTDSFQDNPGWQGKWTVFYWAWTICWSPYVGMFVARISRGRTVREFIGGVLALPAIFGVVWFSIFGRAGI
EVELSNPGFLTQPTVVEGDVPAALFNVLQEYPLTGIVSAFALVIIVIFFITSIDSAALVNDMFATGEENQTPTSYRVMWA
CTIGAVAGSLLIISPSSGIATLQEVVIIVAFPFFLVQFVMMFSLLKGMSEDAAAVRRVQTRQWEKTDTPEKLEEHSSQPA
PGYDDEGNPLPMPALEHDEDGNIVIPGNVVIEGDLGVVGDVVDDPEEAQEMGSRFKIVEQTRPQSRDEYDI
>Mature_551_residues
MYIGGVSLVFIFLMGIFVSRYGRVKLGDDDDDPEHTLIVWFCMLFAGGVGAVLMFWGVAEPINHAFNVPMANEESMSEAA
IVQAFAYTFYHFGIHMWVIMALPGLSLGYFIYKRKLPPRLSSVFSPILGKHIYSTPGKLIDVLAIVGTTFGIAVSVGLGV
LQINAGMNKLWSTPQVSWVQLLIILIITAVACISVASGLDKGIKLLSNINIAMAVALMFFILFTGPTLTLLRFLVESFGI
YASWMPNLMFWTDSFQDNPGWQGKWTVFYWAWTICWSPYVGMFVARISRGRTVREFIGGVLALPAIFGVVWFSIFGRAGI
EVELSNPGFLTQPTVVEGDVPAALFNVLQEYPLTGIVSAFALVIIVIFFITSIDSAALVNDMFATGEENQTPTSYRVMWA
CTIGAVAGSLLIISPSSGIATLQEVVIIVAFPFFLVQFVMMFSLLKGMSEDAAAVRRVQTRQWEKTDTPEKLEEHSSQPA
PGYDDEGNPLPMPALEHDEDGNIVIPGNVVIEGDLGVVGDVVDDPEEAQEMGSRFKIVEQTRPQSRDEYDI

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=559, Percent_Identity=32.9159212880143, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1788102, Length=467, Percent_Identity=26.5524625267666, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786224, Length=438, Percent_Identity=26.027397260274, Blast_Score=148, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60620; Mature: 60620

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]