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The map label for this gene is glnA [H]

Identifier: 145296187

GI number: 145296187

Start: 2325253

End: 2326593

Strand: Reverse

Name: glnA [H]

Synonym: cgR_2106

Alternate gene names: 145296187

Gene position: 2326593-2325253 (Counterclockwise)

Preceding gene: 145296190

Following gene: 145296186

Centisome position: 70.2

GC content: 52.87

Gene sequence:

>1341_bases
ATGAACAGCGAACAGGAATTTGTACTCAGCGCCATTGAAGAACGTGACATTAAGTTTGTGCGTCTATGGTTCACTGACAT
TCTTGGCCACCTGAAGTCAGTGGTTGTGGCTCCTGCAGAACTAGAGTCTGCGTTGGAAGAAGGCATCGGATTCGATGGCT
CAGCTATTGAGGGCTACGCGCGTATCTCGGAAGCGGACACCATTGCCCGCCCAGATCCTTCGACATTCCAGGTCCTCCCA
CTAGAAGCGGGCATCTCAAAACTGCAGGCAGCACGCCTGTTTTGCGATGTCACGATGCCAGACGGACAGCCATCTTTTTC
TGACCCGCGCCAAGTGCTGCGCAGGCAGGTCCAACTAGCTGCAGATGAAGGCTTGACCTGCATGATCTCACCAGAGATTG
AGTTCTATTTGGTGCAAAGCCTTCGCACCAACGGACTGCCACCTGTGCCCACTGACAACGGCGGATATTTCGACCAAGCC
ACATTCAATGAGGCGCCGAATTTCCGTCGAAACGCGATGGTAGCGCTGGAGGAACTCGGCATCCCTGTCGAGTTCTCCCA
CCATGAAACTGCACCTGGCCAGCAAGAAATCGATTTACGCCATGCGGATGCGCTCACCATGGCCGACAACATCATGACCT
TCCGCTACATCATGAAACAGGTGGCAAGGGACCAAGGCGTTGGGGCATCATTTATGCCCAAGCCATTCCAAGAACATGCA
GGCTCCGCCATGCACACGCACATGTCCTTATTTGAGGGCGATACCAACGCGTTCCACGATCCAGACGATTCTTACATGCT
GTCCAAAACCGCAAAACAGTTCATCGCTGGAATCTTGCATCACGCTCCAGAATTCACCGCTGTGACCAACCAGTGGGTCA
ATTCCTACAAACGCATCGTGTACGGAAACGAAGCTCCAACTGCGGCAACCTGGGGTGTATCTAATCGTTCTGCGCTGGTT
CGTGTTCCTACCTACCGTTTGAATAAGGAGGAGTCGCGCCGGGTGGAGGTGCGTCTTCCTGATACCGCTTGTAACCCATA
TTTGGCGTTTTCAGTGATGCTCGGCGCTGGTTTGAAAGGCATTAAAGAAGGTTATGAGCTCGACGAGCCAGCTGAGGACG
ATATCTCCAACTTGAGCTTCCGGGAACGTCGCGCCATGGGCTACAACGATCTGCCAAGCAGCCTTGATCAGGCACTGCGC
CAAATGGAAAAGTCAGAGCTTGTTGCTGACATCCTCGGTGAGCACGTTTTTGAGTTTTTCTTGCGCAATAAGTGGCGTGA
ATGGCGTGACTACCAAGAGCAGATCACTCCGTGGGAGCTCCGAAACAATCTTGATTACTAG

Upstream 100 bases:

>100_bases
CGCGACATCCTTGAGTAACTCTGAGAAAAACTACCCCCGATGCGAGTATAAAAGTGGCAAATGCGCAGTCGATGTCCCAT
CGCTGCGTAGATTAGTTTTC

Downstream 100 bases:

>100_bases
ACTTTTGCACTCCAATGGAAACCCTACGGTGACCCAATTGCGACCCGATAAAGGAGGGGAGAAGCTATGTCAGGACCGTT
AAGAAGTGAACGTAAAGTCG

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNSEQEFVLSAIEERDIKFVRLWFTDILGHLKSVVVAPAELESALEEGIGFDGSAIEGYARISEADTIARPDPSTFQVLP
LEAGISKLQAARLFCDVTMPDGQPSFSDPRQVLRRQVQLAADEGLTCMISPEIEFYLVQSLRTNGLPPVPTDNGGYFDQA
TFNEAPNFRRNAMVALEELGIPVEFSHHETAPGQQEIDLRHADALTMADNIMTFRYIMKQVARDQGVGASFMPKPFQEHA
GSAMHTHMSLFEGDTNAFHDPDDSYMLSKTAKQFIAGILHHAPEFTAVTNQWVNSYKRIVYGNEAPTAATWGVSNRSALV
RVPTYRLNKEESRRVEVRLPDTACNPYLAFSVMLGAGLKGIKEGYELDEPAEDDISNLSFRERRAMGYNDLPSSLDQALR
QMEKSELVADILGEHVFEFFLRNKWREWRDYQEQITPWELRNNLDY

Sequences:

>Translated_446_residues
MNSEQEFVLSAIEERDIKFVRLWFTDILGHLKSVVVAPAELESALEEGIGFDGSAIEGYARISEADTIARPDPSTFQVLP
LEAGISKLQAARLFCDVTMPDGQPSFSDPRQVLRRQVQLAADEGLTCMISPEIEFYLVQSLRTNGLPPVPTDNGGYFDQA
TFNEAPNFRRNAMVALEELGIPVEFSHHETAPGQQEIDLRHADALTMADNIMTFRYIMKQVARDQGVGASFMPKPFQEHA
GSAMHTHMSLFEGDTNAFHDPDDSYMLSKTAKQFIAGILHHAPEFTAVTNQWVNSYKRIVYGNEAPTAATWGVSNRSALV
RVPTYRLNKEESRRVEVRLPDTACNPYLAFSVMLGAGLKGIKEGYELDEPAEDDISNLSFRERRAMGYNDLPSSLDQALR
QMEKSELVADILGEHVFEFFLRNKWREWRDYQEQITPWELRNNLDY
>Mature_446_residues
MNSEQEFVLSAIEERDIKFVRLWFTDILGHLKSVVVAPAELESALEEGIGFDGSAIEGYARISEADTIARPDPSTFQVLP
LEAGISKLQAARLFCDVTMPDGQPSFSDPRQVLRRQVQLAADEGLTCMISPEIEFYLVQSLRTNGLPPVPTDNGGYFDQA
TFNEAPNFRRNAMVALEELGIPVEFSHHETAPGQQEIDLRHADALTMADNIMTFRYIMKQVARDQGVGASFMPKPFQEHA
GSAMHTHMSLFEGDTNAFHDPDDSYMLSKTAKQFIAGILHHAPEFTAVTNQWVNSYKRIVYGNEAPTAATWGVSNRSALV
RVPTYRLNKEESRRVEVRLPDTACNPYLAFSVMLGAGLKGIKEGYELDEPAEDDISNLSFRERRAMGYNDLPSSLDQALR
QMEKSELVADILGEHVFEFFLRNKWREWRDYQEQITPWELRNNLDY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=452, Percent_Identity=24.5575221238938, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1790301, Length=431, Percent_Identity=33.6426914153132, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI87081870, Length=458, Percent_Identity=27.5109170305677, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50382; Mature: 50382

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEQEFVLSAIEERDIKFVRLWFTDILGHLKSVVVAPAELESALEEGIGFDGSAIEGYA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
RISEADTIARPDPSTFQVLPLEAGISKLQAARLFCDVTMPDGQPSFSDPRQVLRRQVQLA
HHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHH
ADEGLTCMISPEIEFYLVQSLRTNGLPPVPTDNGGYFDQATFNEAPNFRRNAMVALEELG
HCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
IPVEFSHHETAPGQQEIDLRHADALTMADNIMTFRYIMKQVARDQGVGASFMPKPFQEHA
CCEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GSAMHTHMSLFEGDTNAFHDPDDSYMLSKTAKQFIAGILHHAPEFTAVTNQWVNSYKRIV
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEE
YGNEAPTAATWGVSNRSALVRVPTYRLNKEESRRVEVRLPDTACNPYLAFSVMLGAGLKG
ECCCCCCCEECCCCCCCEEEECCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHH
IKEGYELDEPAEDDISNLSFRERRAMGYNDLPSSLDQALRQMEKSELVADILGEHVFEFF
HHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKWREWRDYQEQITPWELRNNLDY
HHHHHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MNSEQEFVLSAIEERDIKFVRLWFTDILGHLKSVVVAPAELESALEEGIGFDGSAIEGYA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
RISEADTIARPDPSTFQVLPLEAGISKLQAARLFCDVTMPDGQPSFSDPRQVLRRQVQLA
HHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHH
ADEGLTCMISPEIEFYLVQSLRTNGLPPVPTDNGGYFDQATFNEAPNFRRNAMVALEELG
HCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
IPVEFSHHETAPGQQEIDLRHADALTMADNIMTFRYIMKQVARDQGVGASFMPKPFQEHA
CCEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GSAMHTHMSLFEGDTNAFHDPDDSYMLSKTAKQFIAGILHHAPEFTAVTNQWVNSYKRIV
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEE
YGNEAPTAATWGVSNRSALVRVPTYRLNKEESRRVEVRLPDTACNPYLAFSVMLGAGLKG
ECCCCCCCEECCCCCCCEEEECCCEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHH
IKEGYELDEPAEDDISNLSFRERRAMGYNDLPSSLDQALRQMEKSELVADILGEHVFEFF
HHCCCCCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKWREWRDYQEQITPWELRNNLDY
HHHHHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA