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The map label for this gene is asnB [H]

Identifier: 145296158

GI number: 145296158

Start: 2292526

End: 2294448

Strand: Direct

Name: asnB [H]

Synonym: cgR_2077

Alternate gene names: 145296158

Gene position: 2292526-2294448 (Clockwise)

Preceding gene: 145296155

Following gene: 145296160

Centisome position: 69.17

GC content: 55.02

Gene sequence:

>1923_bases
ATGTGCGGCCTTCTTGGCATATTGACTGCAAATGGGAACGCTGAAGCATTCGTTCCTGCACTCGAGCGGGCCTTGCCATG
CATGCGCCACCGTGGTCCTGACGATGCCGGCACTTGGCATGACGCCGATGCAGCGTTTGGATTCAACCGCCTCTCCATCA
TTGATATTGCACACTCCCACCAACCACTGCGTTGGGGACCTGCGGATGAACCCGACCGCTACGCAATGACTTTCAACGGT
GAGATCTACAACTACGTTGAGCTGCGTAAAGAGCTCTCGGATTTGGGATATACCTTTAATACTTCTGGCGATGGCGAGCC
AATTGTTGTCGGTTTCCACCACTGGGGCGAGTCCGTGGTCGAGCATCTCCGCGGAATGTTCGGCATTGCCATTTGGGATA
CAAAGGAAAAGTCGCTTTTCCTTGCGCGTGATCAGTTCGGCATCAAGCCACTGTTCTACGCAACCACCGAGCATGGCACC
GTGTTCTCCTCAGAGAAGAAGACTATCTTGGAGATGGCCGAGGAGATGAATCTAGATTTGGGCCTTGATAAACGCACCAT
TGAGCACTACGTGGACTTGCAGTACGTGCCCGAGCCAGATACCCTTCACGCGCAGATTTCCCGCTTGGAGTCAGGCTGCA
CCGCAACAGTTCGTCCGGGCGGCAAGCTGGAACAGAAGCGTTACTTCAAGCCTCAGTTCCCAGTACAGAAGGTCGTAAAG
GGTAAGGAGCAGGACCTCTTCGATCGCATTGCCCAGGTGTTGGAGGATAGCGTCGAAAAGCATATGCGTGCCGACGTGAC
CGTAGGCTCGTTCCTTTCCGGCGGCATTGACTCAACCGCAATTGCGGCGCTTGCAAAGCGCCACAACCCTGACCTGCTCA
CCTTCACCACCGGTTTCGAGCGTGAAGGCTACTCGGAGGTCGATGTGGCTGCGGAGTCCGCCGCTGCGATTGGCGCTGAG
CACATCGTGAAGATTGTCTCGCCTGAGGAATACGCCAACGCGATTCCTAAGATCATGTGGTACTTGGATGATCCTGTAGC
TGACCCATCATTGGTCCCGCTGTACTTCGTGGCAGCGGAAGCACGTAAGCACGTCAAGGTTGTGCTGTCTGGCGAGGGCG
CAGATGAGCTGTTCGGTGGATACACCATTTACAAGGAGCCGCTATCGCTTGCTCCATTTGAGAAGATCCCTTCCCCACTA
CGTAAAGGCCTGGGAAAGCTCAGCAAGGTTCTGCCAGACGGCATGAAGGGCAAGTCCCTTCTTGAGCGTGGCTCCATGAC
CATGGAAGAGCGCTACTACGGCAACGCTCGCTCCTTCAATTTCGAGCAGATGCAACGCGTTATTCCATGGGCAAAGCGCG
AATGGGACCACCGCGAGGTCACTGCACCGATCTACGCACAGTCCCGCAACTTTGATCCAGTAGCCCGCATGCAACACCTG
GATCTGTTCACCTGGATGCGCGGCGACATCCTGGTCAAGGCTGACAAGATCAACATGGCGAACTCCCTTGAGCTGCGAGT
TCCATTCTTGGATAAGGAAGTTTTCAAGGTTGCAGAGACCATTCCTTACGATCTGAAGATTGCCAACGGTACCACCAAGT
ACGCGCTGCGCAGGGCACTCGAGCAGATTGTTCCGCCTCACGTTTTGCACCGCAAGAAGCTGGGCTTCCCTGTTCCCATG
CGCCACTGGCTTGCCGGCGATGAGCTGTTCGGTTGGGCGCAGGACACCATCAAGGAATCCGGTACTGAAGATATCTTCAA
CAAGCAGGCTGTGCTGGATATGCTGAACGAGCACCGCGATGGCGTGTCAGATCATTCCCGTCGACTGTGGACTGTTCTGT
CATTTATGGTGTGGCACGGCATTTTTGTGGAAAACCGCATTGATCCACAGATTGAGGACCGCTCCTACCCAGTCGAGCTT
TAA

Upstream 100 bases:

>100_bases
TATCGTTGACACGAGATTTAACAAATGCAGCGTCTTATTTCTTCCAACAAAATTTCTTTGCGATTTAAGGCGCCTTTTAT
TTCAGGAGGATTTTTCATTC

Downstream 100 bases:

>100_bases
GTCTTAAAGCCTAAACCCCCTCCTTCTCAAGGAGGGGGTTTCACTATTTCCTGAGGACAAAGCAATTACGCCAGCAAACA
CAAAAGCTCGGCCGTAGACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIAHSHQPLRWGPADEPDRYAMTFNG
EIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESVVEHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGT
VFSSEKKTILEMAEEMNLDLGLDKRTIEHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVK
GKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAALAKRHNPDLLTFTTGFEREGYSEVDVAAESAAAIGAE
HIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLYFVAAEARKHVKVVLSGEGADELFGGYTIYKEPLSLAPFEKIPSPL
RKGLGKLSKVLPDGMKGKSLLERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHREVTAPIYAQSRNFDPVARMQHL
DLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAETIPYDLKIANGTTKYALRRALEQIVPPHVLHRKKLGFPVPM
RHWLAGDELFGWAQDTIKESGTEDIFNKQAVLDMLNEHRDGVSDHSRRLWTVLSFMVWHGIFVENRIDPQIEDRSYPVEL

Sequences:

>Translated_640_residues
MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIAHSHQPLRWGPADEPDRYAMTFNG
EIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESVVEHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGT
VFSSEKKTILEMAEEMNLDLGLDKRTIEHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVK
GKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAALAKRHNPDLLTFTTGFEREGYSEVDVAAESAAAIGAE
HIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLYFVAAEARKHVKVVLSGEGADELFGGYTIYKEPLSLAPFEKIPSPL
RKGLGKLSKVLPDGMKGKSLLERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHREVTAPIYAQSRNFDPVARMQHL
DLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAETIPYDLKIANGTTKYALRRALEQIVPPHVLHRKKLGFPVPM
RHWLAGDELFGWAQDTIKESGTEDIFNKQAVLDMLNEHRDGVSDHSRRLWTVLSFMVWHGIFVENRIDPQIEDRSYPVEL
>Mature_640_residues
MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIAHSHQPLRWGPADEPDRYAMTFNG
EIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESVVEHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGT
VFSSEKKTILEMAEEMNLDLGLDKRTIEHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVK
GKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAALAKRHNPDLLTFTTGFEREGYSEVDVAAESAAAIGAE
HIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLYFVAAEARKHVKVVLSGEGADELFGGYTIYKEPLSLAPFEKIPSPL
RKGLGKLSKVLPDGMKGKSLLERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHREVTAPIYAQSRNFDPVARMQHL
DLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAETIPYDLKIANGTTKYALRRALEQIVPPHVLHRKKLGFPVPM
RHWLAGDELFGWAQDTIKESGTEDIFNKQAVLDMLNEHRDGVSDHSRRLWTVLSFMVWHGIFVENRIDPQIEDRSYPVEL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=419, Percent_Identity=27.9236276849642, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI168229252, Length=419, Percent_Identity=27.9236276849642, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI168229250, Length=419, Percent_Identity=27.9236276849642, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI296010848, Length=388, Percent_Identity=28.8659793814433, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI296010852, Length=317, Percent_Identity=28.7066246056782, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI296010850, Length=317, Percent_Identity=28.7066246056782, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1786889, Length=403, Percent_Identity=29.2803970223325, Blast_Score=157, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71993933, Length=409, Percent_Identity=29.3398533007335, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=409, Percent_Identity=29.3398533007335, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560178, Length=404, Percent_Identity=28.2178217821782, Blast_Score=119, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321563, Length=429, Percent_Identity=26.1072261072261, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=429, Percent_Identity=25.8741258741259, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45553209, Length=417, Percent_Identity=26.6187050359712, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72419; Mature: 72419

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIAHSH
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEECCCC
QPLRWGPADEPDRYAMTFNGEIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESVV
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHH
EHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGTVFSSEKKTILEMAEEMNLDL
HHHHHHHEEEEEECCCCEEEEEECCCCCCEEEEEECCCCEEECCCHHHHHHHHHHCCCCC
GLDKRTIEHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVK
CCCHHHHHHHHCEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCHHHHHC
GKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAALAKRHNPDLLTFTTGFE
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCC
REGYSEVDVAAESAAAIGAEHIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLYFVAAE
CCCCCHHHHHHHHHHHHCHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ARKHVKVVLSGEGADELFGGYTIYKEPLSLAPFEKIPSPLRKGLGKLSKVLPDGMKGKSL
CCCEEEEEEECCCHHHHHCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHH
LERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHREVTAPIYAQSRNFDPVARMQHL
HHHCCCCHHHHHCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCEECCCCCCHHHHHHHH
DLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAETIPYDLKIANGTTKYALRRAL
HHHHCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
EQIVPPHVLHRKKLGFPVPMRHWLAGDELFGWAQDTIKESGTEDIFNKQAVLDMLNEHRD
HHHCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GVSDHSRRLWTVLSFMVWHGIFVENRIDPQIEDRSYPVEL
CCCHHHHHHHHHHHHHHHHHEHEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIAHSH
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEECCCC
QPLRWGPADEPDRYAMTFNGEIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESVV
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHH
EHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGTVFSSEKKTILEMAEEMNLDL
HHHHHHHEEEEEECCCCEEEEEECCCCCCEEEEEECCCCEEECCCHHHHHHHHHHCCCCC
GLDKRTIEHYVDLQYVPEPDTLHAQISRLESGCTATVRPGGKLEQKRYFKPQFPVQKVVK
CCCHHHHHHHHCEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCHHHHHC
GKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAALAKRHNPDLLTFTTGFE
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCC
REGYSEVDVAAESAAAIGAEHIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLYFVAAE
CCCCCHHHHHHHHHHHHCHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ARKHVKVVLSGEGADELFGGYTIYKEPLSLAPFEKIPSPLRKGLGKLSKVLPDGMKGKSL
CCCEEEEEEECCCHHHHHCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHH
LERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHREVTAPIYAQSRNFDPVARMQHL
HHHCCCCHHHHHCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCEECCCCCCHHHHHHHH
DLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAETIPYDLKIANGTTKYALRRAL
HHHHCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
EQIVPPHVLHRKKLGFPVPMRHWLAGDELFGWAQDTIKESGTEDIFNKQAVLDMLNEHRD
HHHCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
GVSDHSRRLWTVLSFMVWHGIFVENRIDPQIEDRSYPVEL
CCCHHHHHHHHHHHHHHHHHEHEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]