Click here to switch to the map view.

The map label for this gene is glkA [H]

Identifier: 145296147

GI number: 145296147

Start: 2280584

End: 2281555

Strand: Reverse

Name: glkA [H]

Synonym: cgR_2067

Alternate gene names: 145296147

Gene position: 2281555-2280584 (Counterclockwise)

Preceding gene: 145296148

Following gene: 145296146

Centisome position: 68.84

GC content: 55.86

Gene sequence:

>972_bases
ATGCCACAAAAACCGGCCAGTTTCGCGGTGGGCTTTGACATCGGCGGCACCAACATGCGAGCTGGGCTTGTCGACGAATC
CGGGCGCATCGTGACCAGTTTGTCGGCGCCGTCGCCGCGCACGACGCAGGCAATGGAACAGGGGATTTTTGATCTAGTCG
AACAGCTCAAGGCCGAATACCCGGTTGGTGCTGTGGGACTTGCCGTCGCGGGATTTTTGGATCCTGAGTGCGAGGTTGTT
CGATTTGCCCCGCACCTTCCTTGGCGCGATGAGCCAGTGCGTGAAAAGTTGGAAAACCTTTTGGGCCTGCCTGTTCGTTT
GGAACATGATGCCAACTCAGCAGCGTGGGGTGAGCATCGTTTTGGTGCAGCTCAAGGCGCTGACAACTGGGTTTTGTTGG
CACTCGGCACTGGAATTGGTGCAGCGCTGATTGAAAAAGGCGAAATTTACCGTGGTGCATATGGCACGGCACCAGAATTT
GGTCATTTGCGTGTTGTTCGTGGCGGACGCGCATGTGCGTGTGGCAAAGAAGGCTGCCTGGAGCGTTACTGTTCCGGTAC
TGCCTTGGTTTACACTGCGCGTGAATTGGCTTCGCATGGCTCATTCCGCAACAGCGGGCTGTTTGACAAGATCAAAGCCG
ATCCGAATTCCATCAATGGAAAAACGATCACTGCGGCAGCGCGCCAAGAAGACCCACTTGCTCTCGCCGTTCTGGAAGAT
TTCAGCGAGTGGCTGGGCGAAACTTTGGCGATCATTGCTGATGTCCTTGACCCAGGTATGATCATCATTGGTGGCGGACT
GTCCAATGCTGCCGACCTTTATTTGGATCGCTCGGTTAACCACTATTCCACCCGCATCGTCGGCGCAGGATATCGCCCTT
TGGCACGCGTTGCCACAGCTCAGTTGGGTGCGGATGCTGGCATGATCGGTGTCGCTGATCTGGCTCGACGTTCCGTGTTG
GAAGCCAACTAG

Upstream 100 bases:

>100_bases
TTGCAGAGTGAACCACGATGATGGTTGGACAGCTGTTGATAGCTATACTTTGAAAGATTAAATTCACCTAAATCCTGTGT
AGAACGCGAGGGGCACTCTT

Downstream 100 bases:

>100_bases
GTGTTTTTCGGTGGGCTGCGATGACGCATGTCCACCAAAAGAGCCACCCCTTAAAGAAACTAAAAAGTGGTTTTGGTAGC
TTCGCAGCAAAATACACATC

Product: hypothetical protein

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPQKPASFAVGFDIGGTNMRAGLVDESGRIVTSLSAPSPRTTQAMEQGIFDLVEQLKAEYPVGAVGLAVAGFLDPECEVV
RFAPHLPWRDEPVREKLENLLGLPVRLEHDANSAAWGEHRFGAAQGADNWVLLALGTGIGAALIEKGEIYRGAYGTAPEF
GHLRVVRGGRACACGKEGCLERYCSGTALVYTARELASHGSFRNSGLFDKIKADPNSINGKTITAAARQEDPLALAVLED
FSEWLGETLAIIADVLDPGMIIIGGGLSNAADLYLDRSVNHYSTRIVGAGYRPLARVATAQLGADAGMIGVADLARRSVL
EAN

Sequences:

>Translated_323_residues
MPQKPASFAVGFDIGGTNMRAGLVDESGRIVTSLSAPSPRTTQAMEQGIFDLVEQLKAEYPVGAVGLAVAGFLDPECEVV
RFAPHLPWRDEPVREKLENLLGLPVRLEHDANSAAWGEHRFGAAQGADNWVLLALGTGIGAALIEKGEIYRGAYGTAPEF
GHLRVVRGGRACACGKEGCLERYCSGTALVYTARELASHGSFRNSGLFDKIKADPNSINGKTITAAARQEDPLALAVLED
FSEWLGETLAIIADVLDPGMIIIGGGLSNAADLYLDRSVNHYSTRIVGAGYRPLARVATAQLGADAGMIGVADLARRSVL
EAN
>Mature_322_residues
PQKPASFAVGFDIGGTNMRAGLVDESGRIVTSLSAPSPRTTQAMEQGIFDLVEQLKAEYPVGAVGLAVAGFLDPECEVVR
FAPHLPWRDEPVREKLENLLGLPVRLEHDANSAAWGEHRFGAAQGADNWVLLALGTGIGAALIEKGEIYRGAYGTAPEFG
HLRVVRGGRACACGKEGCLERYCSGTALVYTARELASHGSFRNSGLFDKIKADPNSINGKTITAAARQEDPLALAVLEDF
SEWLGETLAIIADVLDPGMIIIGGGLSNAADLYLDRSVNHYSTRIVGAGYRPLARVATAQLGADAGMIGVADLARRSVLE
AN

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=268, Percent_Identity=30.2238805970149, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI298566325, Length=268, Percent_Identity=30.2238805970149, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI4885285, Length=268, Percent_Identity=30.2238805970149, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI190014632, Length=268, Percent_Identity=30.2238805970149, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1786891, Length=318, Percent_Identity=28.3018867924528, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081733, Length=279, Percent_Identity=31.1827956989247, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787363, Length=279, Percent_Identity=30.1075268817204, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787878, Length=226, Percent_Identity=27.8761061946903, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790522, Length=312, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082127, Length=288, Percent_Identity=25.6944444444444, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082230, Length=298, Percent_Identity=26.510067114094, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 34201; Mature: 34070

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKPASFAVGFDIGGTNMRAGLVDESGRIVTSLSAPSPRTTQAMEQGIFDLVEQLKAEY
CCCCCCCEEEEEECCCCCCEECEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PVGAVGLAVAGFLDPECEVVRFAPHLPWRDEPVREKLENLLGLPVRLEHDANSAAWGEHR
CCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
FGAAQGADNWVLLALGTGIGAALIEKGEIYRGAYGTAPEFGHLRVVRGGRACACGKEGCL
CCCCCCCCCEEEEEECCCCCHHHHCCCCEEECCCCCCCCCCCEEEEECCCEECCCHHHHH
ERYCSGTALVYTARELASHGSFRNSGLFDKIKADPNSINGKTITAAARQEDPLALAVLED
HHHHCCCEEEEEHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCEEHHHHHH
FSEWLGETLAIIADVLDPGMIIIGGGLSNAADLYLDRSVNHYSTRIVGAGYRPLARVATA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEECCCCHHHEEEECCCCHHHHHHHHH
QLGADAGMIGVADLARRSVLEAN
HHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQKPASFAVGFDIGGTNMRAGLVDESGRIVTSLSAPSPRTTQAMEQGIFDLVEQLKAEY
CCCCCCEEEEEECCCCCCEECEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PVGAVGLAVAGFLDPECEVVRFAPHLPWRDEPVREKLENLLGLPVRLEHDANSAAWGEHR
CCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
FGAAQGADNWVLLALGTGIGAALIEKGEIYRGAYGTAPEFGHLRVVRGGRACACGKEGCL
CCCCCCCCCEEEEEECCCCCHHHHCCCCEEECCCCCCCCCCCEEEEECCCEECCCHHHHH
ERYCSGTALVYTARELASHGSFRNSGLFDKIKADPNSINGKTITAAARQEDPLALAVLED
HHHHCCCEEEEEHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCEEHHHHHH
FSEWLGETLAIIADVLDPGMIIIGGGLSNAADLYLDRSVNHYSTRIVGAGYRPLARVATA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEECCCCHHHEEEECCCCHHHHHHHHH
QLGADAGMIGVADLARRSVLEAN
HHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]