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The map label for this gene is ansA [H]

Identifier: 145296104

GI number: 145296104

Start: 2229423

End: 2230400

Strand: Direct

Name: ansA [H]

Synonym: cgR_2025

Alternate gene names: 145296104

Gene position: 2229423-2230400 (Clockwise)

Preceding gene: 145296102

Following gene: 145296111

Centisome position: 67.27

GC content: 56.65

Gene sequence:

>978_bases
ATGTCGAAGCAGCACTCCACACCATTAAACAATGATGAAGAACACACTTCCGCTCCTCAAAAGGTTGCGGTAATCACCAC
GGGCGGAACCATCGCCTGTACTTCCGACGCAAATGGGCATCTGCTTCCCACCGTCAGCGGTGCAGACCTGCTTGCGCCAA
TCGCACCACGGTTCAATGGAGCGCAGATCGCTTTCGAAATCCACGAAATCAACCGCCTTGATTCCTCCTCCATGACGTTT
GAGGATCTCGATTCCATCATCGCCACGGTTCATAAGGTGTTGGAGGATCCGGATGTTGTTGGCGTAGTAGTTACCCACGG
CACCGATTCCATGGAAGAGTCCGCCATCGCCGTAGACACCTTCCTTGATGATCCCCGCCCAGTCATTTTCACCGGCGCCC
AAAAACCCTTCGATCATCCCGAAGCCGACGGCCCAAACAACCTTTTCGAAGCCTGCCTCATCGCATCCGACCCCTCCGCT
CGCGGAATTGGTGCACTCATTGTCTTCGGTCACGCCGTCATCCCTGCTCGCGGCTGCGTTAAATGGCACACCTCTGATGA
GCTGGCGTTTGCAACCAACGGCCCTGAAGAACCAGAGCGCCCCGATGCGCTGCCCGTAGCTAAATTGGCGGATGTCTCTG
TCGAAATCATCCCCGCATACCCTGGTGCCACCGGCGCAATGGTGGAAGCTGCCATCGCTGCCGGCGCTCAAGGACTTGTA
GTGGAAGCAATGGGATCAGGCAATGTTGGTTCCCGCATGGGTGATGCCCTAGGTAAAGCACTTGACGCTGGAATTCCCGT
GGTGATGAGCACTAGGGTTCCTCGTGGTGAAGTATCCGGAGTGTATGGCGGTGCAGGTGGAGGTGCGACTTTGGCTGCGA
AGGGCGCTGTGGGATCTCGCTACTTCAGAGCTGGTCAGGCACGTATTTTGCTCGCGATTGCCATTGCCACGGGCGCACAT
CCGGTGACGCTTTACTAA

Upstream 100 bases:

>100_bases
CACGTGATTCATTTGTGACCAACAACCGAAACTGAGCCAGAAGACTGTCAATCCCCTGCTGTGCACATAACAACTGCAGC
TAGTTGATACGCTAGAGCGC

Downstream 100 bases:

>100_bases
TTTCGCCGTTGGTGTTGCATTAGGTTGAGCAGTGAAGACCCCTTTGGCTCTAGAAGGCGATTTAAAGGGTCGGATTTTCA
CATGGACCTTTGGTGTGGAT

Product: hypothetical protein

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSKQHSTPLNNDEEHTSAPQKVAVITTGGTIACTSDANGHLLPTVSGADLLAPIAPRFNGAQIAFEIHEINRLDSSSMTF
EDLDSIIATVHKVLEDPDVVGVVVTHGTDSMEESAIAVDTFLDDPRPVIFTGAQKPFDHPEADGPNNLFEACLIASDPSA
RGIGALIVFGHAVIPARGCVKWHTSDELAFATNGPEEPERPDALPVAKLADVSVEIIPAYPGATGAMVEAAIAAGAQGLV
VEAMGSGNVGSRMGDALGKALDAGIPVVMSTRVPRGEVSGVYGGAGGGATLAAKGAVGSRYFRAGQARILLAIAIATGAH
PVTLY

Sequences:

>Translated_325_residues
MSKQHSTPLNNDEEHTSAPQKVAVITTGGTIACTSDANGHLLPTVSGADLLAPIAPRFNGAQIAFEIHEINRLDSSSMTF
EDLDSIIATVHKVLEDPDVVGVVVTHGTDSMEESAIAVDTFLDDPRPVIFTGAQKPFDHPEADGPNNLFEACLIASDPSA
RGIGALIVFGHAVIPARGCVKWHTSDELAFATNGPEEPERPDALPVAKLADVSVEIIPAYPGATGAMVEAAIAAGAQGLV
VEAMGSGNVGSRMGDALGKALDAGIPVVMSTRVPRGEVSGVYGGAGGGATLAAKGAVGSRYFRAGQARILLAIAIATGAH
PVTLY
>Mature_324_residues
SKQHSTPLNNDEEHTSAPQKVAVITTGGTIACTSDANGHLLPTVSGADLLAPIAPRFNGAQIAFEIHEINRLDSSSMTFE
DLDSIIATVHKVLEDPDVVGVVVTHGTDSMEESAIAVDTFLDDPRPVIFTGAQKPFDHPEADGPNNLFEACLIASDPSAR
GIGALIVFGHAVIPARGCVKWHTSDELAFATNGPEEPERPDALPVAKLADVSVEIIPAYPGATGAMVEAAIAAGAQGLVV
EAMGSGNVGSRMGDALGKALDAGIPVVMSTRVPRGEVSGVYGGAGGGATLAAKGAVGSRYFRAGQARILLAIAIATGAHP
VTLY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=240, Percent_Identity=31.25, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788065, Length=300, Percent_Identity=28, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193207372, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=26.5671641791045, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=26.5671641791045, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=26.5671641791045, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=26.5671641791045, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320527, Length=336, Percent_Identity=25.5952380952381, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24645530, Length=313, Percent_Identity=28.1150159744409, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639609, Length=319, Percent_Identity=25.3918495297806, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33299; Mature: 33168

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQHSTPLNNDEEHTSAPQKVAVITTGGTIACTSDANGHLLPTVSGADLLAPIAPRFNG
CCCCCCCCCCCCHHHCCCCCEEEEEEECCEEEEECCCCCCEEEEECCCHHHHHCCCCCCC
AQIAFEIHEINRLDSSSMTFEDLDSIIATVHKVLEDPDVVGVVVTHGTDSMEESAIAVDT
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEH
FLDDPRPVIFTGAQKPFDHPEADGPNNLFEACLIASDPSARGIGALIVFGHAVIPARGCV
HCCCCCCEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCEEEEEECCEEECCCCEE
KWHTSDELAFATNGPEEPERPDALPVAKLADVSVEIIPAYPGATGAMVEAAIAAGAQGLV
EECCCCCEEEECCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEE
VEAMGSGNVGSRMGDALGKALDAGIPVVMSTRVPRGEVSGVYGGAGGGATLAAKGAVGSR
EEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCCCCEEEECCCCCHH
YFRAGQARILLAIAIATGAHPVTLY
HHHCCCCEEEEEEEEECCCCEEEEC
>Mature Secondary Structure 
SKQHSTPLNNDEEHTSAPQKVAVITTGGTIACTSDANGHLLPTVSGADLLAPIAPRFNG
CCCCCCCCCCCHHHCCCCCEEEEEEECCEEEEECCCCCCEEEEECCCHHHHHCCCCCCC
AQIAFEIHEINRLDSSSMTFEDLDSIIATVHKVLEDPDVVGVVVTHGTDSMEESAIAVDT
EEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEH
FLDDPRPVIFTGAQKPFDHPEADGPNNLFEACLIASDPSARGIGALIVFGHAVIPARGCV
HCCCCCCEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCEEEEEECCEEECCCCEE
KWHTSDELAFATNGPEEPERPDALPVAKLADVSVEIIPAYPGATGAMVEAAIAAGAQGLV
EECCCCCEEEECCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEE
VEAMGSGNVGSRMGDALGKALDAGIPVVMSTRVPRGEVSGVYGGAGGGATLAAKGAVGSR
EEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCCCCEEEECCCCCHH
YFRAGQARILLAIAIATGAHPVTLY
HHHCCCCEEEEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]