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The map label for this gene is hisS [H]

Identifier: 145295768

GI number: 145295768

Start: 1886436

End: 1887725

Strand: Reverse

Name: hisS [H]

Synonym: cgR_1694

Alternate gene names: 145295768

Gene position: 1887725-1886436 (Counterclockwise)

Preceding gene: 145295769

Following gene: 145295767

Centisome position: 56.96

GC content: 55.43

Gene sequence:

>1290_bases
GTGAGTCAAAACAAGTCCAAGTCTGAAAAACTTCAGTCATTTGCTGCACCCAAGGGTGTTCCTGATTACGCCCCACCAAA
ATCTGCAGCGTTTTTAGCAGTCCGTGATGCCTTTGTTGATCAAGCGCATAAGGCCGGGTTTGAGCATATTGAACTGCCGA
TCTTTGAAGACACCGGCTTGTTTGCGCGTGGTGTTGGTGAGTCCACCGACGTGGTGAGCAAGGAAATGTACACCTTCGCT
GATCGTGGCGAGCGCTCTGTCACACTGCGCCCAGAAGGCACCGCAGGTGTGATGCGTGCAGTTATTGAACACAGCCTGGA
CCGTGGACAGCTTCCCGTAAAGCTGAACTATGCGGGACCATTCTTCCGTTATGAGCGTCCACAGGCGGGTCGTTACCGTC
AGCTTCAGCAAGTTGGCGTGGAGGCCATTGGTGTGGATGATCCAGCGCTCGACGCAGAGATCATTGCGTTGGCGGATCGT
TCGTACCGCAGCCTGGGTTTGAAGGATTTCCGCCTGGAGCTCACCAGCTTGGGTGATCGTCACTGCCGTCCGGAGTACCG
CCAGAAGCTGCAGGATTTCTTGTTTGCACTTCCCTTGGATGAGGAAACCCGCAAGCGCGCAGAGATCAACCCACTTCGCG
TGTTGGATGATAAGCGTCCTGAAGTCCAAGAGATGACTGCGGATGCACCATTGATGCTGGATCACCTTGATGCAGAGTGC
CGTGAGCACTTTGAAACAGTGACTGGTTTGCTCGATGACATGGGTGTTCCATATGTGATTAACCCACGCATGGTTCGTGG
TTTGGATTATTACACCAAGACTTGTTTTGAGTTCGTTCACGATGGCCTGGGCGCACAGTCTGGCATTGGTGGCGGCGGAC
GCTACGACGGTCTGATGGCACAGCTTGGCGGACAGGATCTGTCTGGCATCGGCTATGGCCTGGGTGTGGATCGCACCATG
TTGGCTCTGGAAGCTGAAGGCGTGACCGTCGGTGCTGAGCGTCGCGTTGATGTGTACGGCGTTCCACTGGGCAAGGATGC
CAAGAAGGCGCTTGCTGGAATCGTGAACAAGCTGCGCGCTGCGGGCATTTCCACCGATATGTCTTACGGCGACCGTGGCC
TGAAGGGTGCCATGAAGGGCGCTGACCGCTCCAACGCGTTGTACACCTTGGTGCTGGGCGAGCAGGAGCTGGAGAACAAC
ACCATCGCGGTGAAGGATATGCGTGCGCATGAGCAGCACGATGTCGCATTGGACGAGGTTGTGGCCTTTTTGCAGGGGAA
ACTTGTTTAA

Upstream 100 bases:

>100_bases
AACCACCACCATGCGTGCAGAACGCACTGGTAACCCTTTCTTGCTGGCACTGTAGGGCTAAGTTCCGCACTACTTCTTTG
AATAGGTATCGTTAATAATC

Downstream 100 bases:

>100_bases
ATAATTCATAAGTAAAAAACCGTCAATTCACGATGTGGGTTGGCGGTTTTCCTATTAGGCTCACTTTTATGACGAGCGCA
CACTTTGAATCCCGCCGGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSQNKSKSEKLQSFAAPKGVPDYAPPKSAAFLAVRDAFVDQAHKAGFEHIELPIFEDTGLFARGVGESTDVVSKEMYTFA
DRGERSVTLRPEGTAGVMRAVIEHSLDRGQLPVKLNYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEIIALADR
SYRSLGLKDFRLELTSLGDRHCRPEYRQKLQDFLFALPLDEETRKRAEINPLRVLDDKRPEVQEMTADAPLMLDHLDAEC
REHFETVTGLLDDMGVPYVINPRMVRGLDYYTKTCFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQDLSGIGYGLGVDRTM
LALEAEGVTVGAERRVDVYGVPLGKDAKKALAGIVNKLRAAGISTDMSYGDRGLKGAMKGADRSNALYTLVLGEQELENN
TIAVKDMRAHEQHDVALDEVVAFLQGKLV

Sequences:

>Translated_429_residues
MSQNKSKSEKLQSFAAPKGVPDYAPPKSAAFLAVRDAFVDQAHKAGFEHIELPIFEDTGLFARGVGESTDVVSKEMYTFA
DRGERSVTLRPEGTAGVMRAVIEHSLDRGQLPVKLNYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEIIALADR
SYRSLGLKDFRLELTSLGDRHCRPEYRQKLQDFLFALPLDEETRKRAEINPLRVLDDKRPEVQEMTADAPLMLDHLDAEC
REHFETVTGLLDDMGVPYVINPRMVRGLDYYTKTCFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQDLSGIGYGLGVDRTM
LALEAEGVTVGAERRVDVYGVPLGKDAKKALAGIVNKLRAAGISTDMSYGDRGLKGAMKGADRSNALYTLVLGEQELENN
TIAVKDMRAHEQHDVALDEVVAFLQGKLV
>Mature_428_residues
SQNKSKSEKLQSFAAPKGVPDYAPPKSAAFLAVRDAFVDQAHKAGFEHIELPIFEDTGLFARGVGESTDVVSKEMYTFAD
RGERSVTLRPEGTAGVMRAVIEHSLDRGQLPVKLNYAGPFFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEIIALADRS
YRSLGLKDFRLELTSLGDRHCRPEYRQKLQDFLFALPLDEETRKRAEINPLRVLDDKRPEVQEMTADAPLMLDHLDAECR
EHFETVTGLLDDMGVPYVINPRMVRGLDYYTKTCFEFVHDGLGAQSGIGGGGRYDGLMAQLGGQDLSGIGYGLGVDRTML
ALEAEGVTVGAERRVDVYGVPLGKDAKKALAGIVNKLRAAGISTDMSYGDRGLKGAMKGADRSNALYTLVLGEQELENNT
IAVKDMRAHEQHDVALDEVVAFLQGKLV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=44.0758293838863, Blast_Score=340, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI71993686, Length=462, Percent_Identity=23.5930735930736, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=462, Percent_Identity=23.5930735930736, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47141; Mature: 47010

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNKSKSEKLQSFAAPKGVPDYAPPKSAAFLAVRDAFVDQAHKAGFEHIELPIFEDTGL
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FARGVGESTDVVSKEMYTFADRGERSVTLRPEGTAGVMRAVIEHSLDRGQLPVKLNYAGP
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCC
FFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEIIALADRSYRSLGLKDFRLELTSLGDR
CCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHCCCC
HCRPEYRQKLQDFLFALPLDEETRKRAEINPLRVLDDKRPEVQEMTADAPLMLDHLDAEC
CCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEECCCCCCHHHHHCCCCCHHHHHCCHHH
REHFETVTGLLDDMGVPYVINPRMVRGLDYYTKTCFEFVHDGLGAQSGIGGGGRYDGLMA
HHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QLGGQDLSGIGYGLGVDRTMLALEAEGVTVGAERRVDVYGVPLGKDAKKALAGIVNKLRA
HHCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
AGISTDMSYGDRGLKGAMKGADRSNALYTLVLGEQELENNTIAVKDMRAHEQHDVALDEV
CCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCHHHCCCEEEEEECHHHHHHCHHHHHH
VAFLQGKLV
HHHHHCCCC
>Mature Secondary Structure 
SQNKSKSEKLQSFAAPKGVPDYAPPKSAAFLAVRDAFVDQAHKAGFEHIELPIFEDTGL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FARGVGESTDVVSKEMYTFADRGERSVTLRPEGTAGVMRAVIEHSLDRGQLPVKLNYAGP
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCC
FFRYERPQAGRYRQLQQVGVEAIGVDDPALDAEIIALADRSYRSLGLKDFRLELTSLGDR
CCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHCCCC
HCRPEYRQKLQDFLFALPLDEETRKRAEINPLRVLDDKRPEVQEMTADAPLMLDHLDAEC
CCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEECCCCCCHHHHHCCCCCHHHHHCCHHH
REHFETVTGLLDDMGVPYVINPRMVRGLDYYTKTCFEFVHDGLGAQSGIGGGGRYDGLMA
HHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QLGGQDLSGIGYGLGVDRTMLALEAEGVTVGAERRVDVYGVPLGKDAKKALAGIVNKLRA
HHCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHH
AGISTDMSYGDRGLKGAMKGADRSNALYTLVLGEQELENNTIAVKDMRAHEQHDVALDEV
CCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCHHHCCCEEEEEECHHHHHHCHHHHHH
VAFLQGKLV
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]