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The map label for this gene is aspS

Identifier: 145295757

GI number: 145295757

Start: 1871433

End: 1873259

Strand: Reverse

Name: aspS

Synonym: cgR_1683

Alternate gene names: 145295757

Gene position: 1873259-1871433 (Counterclockwise)

Preceding gene: 145295759

Following gene: 145295756

Centisome position: 56.52

GC content: 58.51

Gene sequence:

>1827_bases
GTGCTGCGCACTCATCTCTCAGGCGAGCTTCGTAAAGAAAACGCAGGCCAGTCAGTAACCCTGACCGGCTGGGTCAACCG
TCGCCGTGACCACGGTGGCGTGATCTTCATCGATCTTCGCGACCGTACTGGCATTGCCCAGGTTGTTTTCCGCAATGAGG
ACGTTGCTGAGCGTGCACACGCACTGCGCAGCGAATTCGTCCTACGCGTCACCGGCGTCGTCGAAGAGCGCCCAGAAGGC
TCTCAGAACCCTAACCTTGCTTCCGGCGACATTGAAGTCAGCGTCACCGAATTTGAGGTACTCAACGAGTCCGCTCCACT
TCCTTTCCAGATCGAGGATTCCTCCTCTGCTGGTGAAGTCGGCGAAGAGACCCGTCTGAAGTACCGCTACCTAGACCTGC
GTCGCCCAGTTCAGGCGAACGCCCTGCGTCTGCGCTCTGCTGCCAACAAGGCTGCACGCACCGTGTTGGACAGCCACGAT
TTTACCGAGATCGAGACCCCAACCTTGACTCGTTCCACCCCAGAAGGCGCACGTGACTTCCTGGTGCCAGCTCGTCTGCG
CCCAGGCACTTTCTACGCATTGCCACAGTCCCCACAGCTGTTCAAGCAGCTGCTGCAGGTTGCTGGCATGGAGCGCTACT
ACCAGATCGCTCGTTGCTACCGCGATGAGGATTTCCGTGCAGACCGCCAGCCAGAGTTCACCCAGCTGGACGTTGAAATG
TCCTTCGTGGACCAGGATGATGTCATCGCACTGGGCGAGGAGATCATCTCTGAGGTGTGGAAGCTGATCGGATATGAGAT
CAAGACTCCAATCCCACGCATGACCTACGCCGATGCAATGCGTCGCTACGGCTCCGACAAGCCAGACCTGCGCTTCGACA
TCGAAATCACCGAGTGCACCGAGTTCTTCCAAGACACCACATTCCGTGTGTTCAAGAACGAATATGTCGGCGCAGTTGTC
ATGACCGGTGGTGCTTCCCAGCCTCGCCGTCAGCTCGACGCATGGCAGGAATGGGCCAAGCAGCGCGGTGCTAAGGGACT
TGCTTACATCCTCGTTGGTGAAGACGGTGAGCTGTCCGGACCTGTAGCTAAGAACATCACCGACGCTGAGCGCGCAGGAA
TCGCAGCTCACGTTGGCGCACAGCCAGGCGACTGCATCTTCTTCGCAGCAGGAGACACCAAGTCTTCCCGCGCACTGCTC
GGTGCGGCTCGTGGCGAGATCGCTAAGAAGCTTGACCTGATCAAGGAAGGCGACTGGGCATTCACCTGGATCGTTGACGC
TCCAATGTTCGAGCCAGCAGCAGATGCCACCGCATCCGGTGACGTTGCACTGGGTAACTCCAAGTGGACCGCAGTCCACC
ACGCCTTCACCTCACCTAAGCCAGAGTTCCTAGATAACTTTGACACCAACCCAGGTGACGCACTTGCTTACGCATACGAC
ATCGTGTGCAACGGCAACGAAATCGGTGGCGGTTCCATCCGTATCCACCAGCGCGACGTTCAGGAACGCGTTTTCGAGGT
CATGGGCATCACCGGTGAAGAAGCACGCGAGAAGTTCGGCTTCCTGCTAGACGCTTTCGCTTTCGGCGCACCTCCACACG
GCGGAATCGCATTCGGCTGGGACCGCATCGTGTCCTTGCTGGGCGGCTTTGACTCCATCCGCGACGTCATCGCGTTCCCT
AAGTCCGGTGGCGGAATCGACCCACTGACTGACGCTCCTGCAGCAATCACCCCGCAGCAGCGTAAGGAAGCTGGCATCGA
TGCAAAGCCAAAGCCTAAGGCGGAAGCACAGGCAGAGGCGCAGGCTGAAGAGTCTGCTGAGAAGTAA

Upstream 100 bases:

>100_bases
TCTGGGCTGCATGCCCGCCCTTTTTGGGTGGTTGAGCTTATTGTTTAAGGCTCCAGAAACCATCGACTGATCAAAACCAA
GCGGAAGGACTTCCACCAAC

Downstream 100 bases:

>100_bases
CTAAGAATGAAATGGGGGTGAAGTTATTCAACTCACCCCCATTTTCTATGGTGCTCTGTACCATAGAATGAGGAAATATG
AGCTTCCGCGCCGATCAAAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MLRTHLSGELRKENAGQSVTLTGWVNRRRDHGGVIFIDLRDRTGIAQVVFRNEDVAERAHALRSEFVLRVTGVVEERPEG
SQNPNLASGDIEVSVTEFEVLNESAPLPFQIEDSSSAGEVGEETRLKYRYLDLRRPVQANALRLRSAANKAARTVLDSHD
FTEIETPTLTRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEM
SFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECTEFFQDTTFRVFKNEYVGAVV
MTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALL
GAARGEIAKKLDLIKEGDWAFTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYD
IVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFDSIRDVIAFP
KSGGGIDPLTDAPAAITPQQRKEAGIDAKPKPKAEAQAEAQAEESAEK

Sequences:

>Translated_608_residues
MLRTHLSGELRKENAGQSVTLTGWVNRRRDHGGVIFIDLRDRTGIAQVVFRNEDVAERAHALRSEFVLRVTGVVEERPEG
SQNPNLASGDIEVSVTEFEVLNESAPLPFQIEDSSSAGEVGEETRLKYRYLDLRRPVQANALRLRSAANKAARTVLDSHD
FTEIETPTLTRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEM
SFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECTEFFQDTTFRVFKNEYVGAVV
MTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALL
GAARGEIAKKLDLIKEGDWAFTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYD
IVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFDSIRDVIAFP
KSGGGIDPLTDAPAAITPQQRKEAGIDAKPKPKAEAQAEAQAEESAEK
>Mature_608_residues
MLRTHLSGELRKENAGQSVTLTGWVNRRRDHGGVIFIDLRDRTGIAQVVFRNEDVAERAHALRSEFVLRVTGVVEERPEG
SQNPNLASGDIEVSVTEFEVLNESAPLPFQIEDSSSAGEVGEETRLKYRYLDLRRPVQANALRLRSAANKAARTVLDSHD
FTEIETPTLTRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEM
SFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECTEFFQDTTFRVFKNEYVGAVV
MTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSGPVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALL
GAARGEIAKKLDLIKEGDWAFTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYD
IVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGWDRIVSLLGGFDSIRDVIAFP
KSGGGIDPLTDAPAAITPQQRKEAGIDAKPKPKAEAQAEAQAEESAEK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=603, Percent_Identity=38.6401326699834, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI45439306, Length=250, Percent_Identity=28, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI4758762, Length=283, Percent_Identity=26.8551236749117, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=26.8774703557312, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=26.8774703557312, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=602, Percent_Identity=46.843853820598, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789256, Length=317, Percent_Identity=25.8675078864353, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790571, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=608, Percent_Identity=34.703947368421, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=28.4172661870504, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71984122, Length=250, Percent_Identity=27.2, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=170, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=170, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=627, Percent_Identity=33.9712918660287, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321807, Length=268, Percent_Identity=27.6119402985075, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=280, Percent_Identity=27.1428571428571, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=264, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=37.5827814569536, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=254, Percent_Identity=28.3464566929134, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921528, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640851, Length=258, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640849, Length=258, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_CORGB (A4QEL0)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138578.1
- ProteinModelPortal:   A4QEL0
- SMR:   A4QEL0
- STRING:   A4QEL0
- GeneID:   4992634
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1683
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67054; Mature: 67054

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTHLSGELRKENAGQSVTLTGWVNRRRDHGGVIFIDLRDRTGIAQVVFRNEDVAERAH
CCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
ALRSEFVLRVTGVVEERPEGSQNPNLASGDIEVSVTEFEVLNESAPLPFQIEDSSSAGEV
HHHHHHHEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCCCCCCC
GEETRLKYRYLDLRRPVQANALRLRSAANKAARTVLDSHDFTEIETPTLTRSTPEGARDF
CHHHHEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHC
LVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECT
EECCCCCHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHH
EFFQDTTFRVFKNEYVGAVVMTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSG
HHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALLGAARGEIAKKLDLIKEGDWA
CHHCCCCHHHHCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYD
EEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEE
IVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGW
EEECCCEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECH
DRIVSLLGGFDSIRDVIAFPKSGGGIDPLTDAPAAITPQQRKEAGIDAKPKPKAEAQAEA
HHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
QAEESAEK
HHHHCCCC
>Mature Secondary Structure
MLRTHLSGELRKENAGQSVTLTGWVNRRRDHGGVIFIDLRDRTGIAQVVFRNEDVAERAH
CCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
ALRSEFVLRVTGVVEERPEGSQNPNLASGDIEVSVTEFEVLNESAPLPFQIEDSSSAGEV
HHHHHHHEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCCCCCCC
GEETRLKYRYLDLRRPVQANALRLRSAANKAARTVLDSHDFTEIETPTLTRSTPEGARDF
CHHHHEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHC
LVPARLRPGTFYALPQSPQLFKQLLQVAGMERYYQIARCYRDEDFRADRQPEFTQLDVEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDQDDVIALGEEIISEVWKLIGYEIKTPIPRMTYADAMRRYGSDKPDLRFDIEITECT
EECCCCCHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHH
EFFQDTTFRVFKNEYVGAVVMTGGASQPRRQLDAWQEWAKQRGAKGLAYILVGEDGELSG
HHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNITDAERAGIAAHVGAQPGDCIFFAAGDTKSSRALLGAARGEIAKKLDLIKEGDWA
CHHCCCCHHHHCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FTWIVDAPMFEPAADATASGDVALGNSKWTAVHHAFTSPKPEFLDNFDTNPGDALAYAYD
EEEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEE
IVCNGNEIGGGSIRIHQRDVQERVFEVMGITGEEAREKFGFLLDAFAFGAPPHGGIAFGW
EEECCCEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECH
DRIVSLLGGFDSIRDVIAFPKSGGGIDPLTDAPAAITPQQRKEAGIDAKPKPKAEAQAEA
HHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH
QAEESAEK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA