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The map label for this gene is carA [H]

Identifier: 145295732

GI number: 145295732

Start: 1845864

End: 1847045

Strand: Reverse

Name: carA [H]

Synonym: cgR_1659

Alternate gene names: 145295732

Gene position: 1847045-1845864 (Counterclockwise)

Preceding gene: 145295733

Following gene: 145295731

Centisome position: 55.73

GC content: 58.29

Gene sequence:

>1182_bases
GTGAGTAAAGACACCACCACCTACCAGGGAGTCACCGAGATCGGATCCGTTCCGGCATACCTGGTTCTTGCAGACGGACG
CACCTTCACCGGATTTGGCTTTGGAGCTATCGGCACCACCCTTGGTGAGGCAGTGTTCACCACCGCCATGACCGGTTACC
AGGAAACCATGACCGATCCTTCCTACCACCGCCAGATCGTTGTGGCTACCGCACCGCAGATTGGCAACACCGGCTGGAAC
GATGAGGACAACGAGTCCCGCGACGGCAAGATTTGGGTTGCAGGCCTTGTTATCCGCGACCTCGCAGCACGTGTGTCCAA
CTGGCGCGCCACCACCTCCTTGCAACAGGAAATGGCAGACCAGGGCATCGTCGGCATCGGCGGAATCGACACCCGCGCAC
TGGTTCGCCACCTGCGCAACGAAGGTTCCATCGCAGCGGGCATCTTCTCCGGCGCTGACGCACAGCGCCCAGTTGAAGAA
CTCGTAGAGATCGTCAAGAATCAGCCAGCAATGGCCGGCGCAAACCTCTCCGTTGAGGTCTCTGCTGATGAAACCTACGT
CATCGAAGCTGAAGGCGAAGAGCGCCACACCGTCGTGGCCTACGACCTGGGCATTAAGCAAAACACCCCACGTCGTTTCT
CTGCACGCGGTGTTCGCACCGTCATCGTGCCTGCTGAAACCCCATTCGAGGACATCAAGCAGTACAACCCATCAGGCGTG
TTTATCTCCAATGGCCCTGGCGACCCTGCAGCAGCAGACGTCATGGTTGATATCGTCCGCGAAGTTCTGGAAGCCGACAT
TCCATTCTTCGGCATCTGCTTCGGCAACCAGATCCTCGGCCGCGCATTCGGCATGGAGACCTACAAGCTGAAGTTTGGCC
ACCGCGGCATCAACGTTCCAGTGAAGAACCACATCACCGGCAAGATCGACATCACCGCCCAGAACCACGGCTTCGCACTC
AAGGGCGAAGCAGGCCAGGAATTCGACACCGATTTCGGCACTGCAATTGTCACCCACACCTGCCTCAACGACGGCGTCGT
TGAAGGTGTTGCGCTGAAGTCCGGTCGCGCATACTCCGTTCAGTACCACCCAGAGGCCGCTGCCGGCCCAAATGATGCAA
GCACCCTGTTTGACCAGTTTGTTGAGCTGATGGATGCAGACGCTCAGAAGAAAGGCGCATAA

Upstream 100 bases:

>100_bases
GTTGCATATAATGCAACATATTGAATAAACTACATTCAGGGTTATCAACCAGCCAATTTCTTTTAAAAAGGCAGACACAC
GAAAGGCGACAACAGTCACC

Downstream 100 bases:

>100_bases
ATAACATGCCAAAGCGTTCAGATATTAACCACGTCCTCGTCATCGGTTCCGGCCCCATCGTCATTGGTCAGGCATGTGAA
TTCGACTACTCCGGCACCCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSKDTTTYQGVTEIGSVPAYLVLADGRTFTGFGFGAIGTTLGEAVFTTAMTGYQETMTDPSYHRQIVVATAPQIGNTGWN
DEDNESRDGKIWVAGLVIRDLAARVSNWRATTSLQQEMADQGIVGIGGIDTRALVRHLRNEGSIAAGIFSGADAQRPVEE
LVEIVKNQPAMAGANLSVEVSADETYVIEAEGEERHTVVAYDLGIKQNTPRRFSARGVRTVIVPAETPFEDIKQYNPSGV
FISNGPGDPAAADVMVDIVREVLEADIPFFGICFGNQILGRAFGMETYKLKFGHRGINVPVKNHITGKIDITAQNHGFAL
KGEAGQEFDTDFGTAIVTHTCLNDGVVEGVALKSGRAYSVQYHPEAAAGPNDASTLFDQFVELMDADAQKKGA

Sequences:

>Translated_393_residues
MSKDTTTYQGVTEIGSVPAYLVLADGRTFTGFGFGAIGTTLGEAVFTTAMTGYQETMTDPSYHRQIVVATAPQIGNTGWN
DEDNESRDGKIWVAGLVIRDLAARVSNWRATTSLQQEMADQGIVGIGGIDTRALVRHLRNEGSIAAGIFSGADAQRPVEE
LVEIVKNQPAMAGANLSVEVSADETYVIEAEGEERHTVVAYDLGIKQNTPRRFSARGVRTVIVPAETPFEDIKQYNPSGV
FISNGPGDPAAADVMVDIVREVLEADIPFFGICFGNQILGRAFGMETYKLKFGHRGINVPVKNHITGKIDITAQNHGFAL
KGEAGQEFDTDFGTAIVTHTCLNDGVVEGVALKSGRAYSVQYHPEAAAGPNDASTLFDQFVELMDADAQKKGA
>Mature_392_residues
SKDTTTYQGVTEIGSVPAYLVLADGRTFTGFGFGAIGTTLGEAVFTTAMTGYQETMTDPSYHRQIVVATAPQIGNTGWND
EDNESRDGKIWVAGLVIRDLAARVSNWRATTSLQQEMADQGIVGIGGIDTRALVRHLRNEGSIAAGIFSGADAQRPVEEL
VEIVKNQPAMAGANLSVEVSADETYVIEAEGEERHTVVAYDLGIKQNTPRRFSARGVRTVIVPAETPFEDIKQYNPSGVF
ISNGPGDPAAADVMVDIVREVLEADIPFFGICFGNQILGRAFGMETYKLKFGHRGINVPVKNHITGKIDITAQNHGFALK
GEAGQEFDTDFGTAIVTHTCLNDGVVEGVALKSGRAYSVQYHPEAAAGPNDASTLFDQFVELMDADAQKKGA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=373, Percent_Identity=38.0697050938338, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI169790915, Length=378, Percent_Identity=36.2433862433862, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI21361331, Length=377, Percent_Identity=36.0742705570292, Blast_Score=198, Evalue=7e-51,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=45.1443569553806, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI193204318, Length=375, Percent_Identity=36.8, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=35.8056265984655, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=36.4556962025316, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=36.2694300518135, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=36.2694300518135, Blast_Score=215, Evalue=3e-56,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42139; Mature: 42007

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDTTTYQGVTEIGSVPAYLVLADGRTFTGFGFGAIGTTLGEAVFTTAMTGYQETMTDP
CCCCCCCCCCHHHHCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCC
SYHRQIVVATAPQIGNTGWNDEDNESRDGKIWVAGLVIRDLAARVSNWRATTSLQQEMAD
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
QGIVGIGGIDTRALVRHLRNEGSIAAGIFSGADAQRPVEELVEIVKNQPAMAGANLSVEV
CCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
SADETYVIEAEGEERHTVVAYDLGIKQNTPRRFSARGVRTVIVPAETPFEDIKQYNPSGV
ECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCEE
FISNGPGDPAAADVMVDIVREVLEADIPFFGICFGNQILGRAFGMETYKLKFGHRGINVP
EEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCEEEEEEECCCCCCCC
VKNHITGKIDITAQNHGFALKGEAGQEFDTDFGTAIVTHTCLNDGVVEGVALKSGRAYSV
CCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCEEEE
QYHPEAAAGPNDASTLFDQFVELMDADAQKKGA
EECCCCCCCCCHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SKDTTTYQGVTEIGSVPAYLVLADGRTFTGFGFGAIGTTLGEAVFTTAMTGYQETMTDP
CCCCCCCCCHHHHCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCCC
SYHRQIVVATAPQIGNTGWNDEDNESRDGKIWVAGLVIRDLAARVSNWRATTSLQQEMAD
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
QGIVGIGGIDTRALVRHLRNEGSIAAGIFSGADAQRPVEELVEIVKNQPAMAGANLSVEV
CCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEE
SADETYVIEAEGEERHTVVAYDLGIKQNTPRRFSARGVRTVIVPAETPFEDIKQYNPSGV
ECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCEE
FISNGPGDPAAADVMVDIVREVLEADIPFFGICFGNQILGRAFGMETYKLKFGHRGINVP
EEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCEEEEEEECCCCCCCC
VKNHITGKIDITAQNHGFALKGEAGQEFDTDFGTAIVTHTCLNDGVVEGVALKSGRAYSV
CCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCEEEE
QYHPEAAAGPNDASTLFDQFVELMDADAQKKGA
EECCCCCCCCCHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]