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The map label for this gene is ribE [H]

Identifier: 145295717

GI number: 145295717

Start: 1829820

End: 1830455

Strand: Reverse

Name: ribE [H]

Synonym: cgR_1644

Alternate gene names: 145295717

Gene position: 1830455-1829820 (Counterclockwise)

Preceding gene: 145295718

Following gene: 145295716

Centisome position: 55.23

GC content: 53.93

Gene sequence:

>636_bases
ATGTTCACAGGTATCGTCGAGGAGCTTGGCTCCGTTGCAGGCGTGGAACATCTGGGAGATTCCATCCGGATGCAGATTTC
CGCGTCCACCGTTTTAGAGGGTGTACATTTGGGAGATTCCATTTCCGTCAATGGTGTGTGCTTGACAGTGGCGTCCTTTG
ACGAGGGACATTTCACTGCAGACCTCATGCAGGAAACCTTAGATCGCAGCTCCCTGGGCGCATTATCCACCGGTAGCAAA
GTTAACCTTGAGCGCGCCATGGCAGCCGATGGCCGTCTGGGTGGACACATCATGCAAGGCCATGTTGATGCCACCACCAC
GCTGATCAAGCGCACCAGCTCAGAGAACTGGGATGTTCTGCGTTTTGAGCTGCCAGCTGATTTGGCTCGCTATGTGGTGG
AAAAAGGCTCCATCGCACTCAATGGCACATCCTTGACTGTATCGTCTTTGGGCGATGATTGGTTTGAGGTTTCCCTGATT
CCCACCACCTTGCGCGACACCACCCACGGCGAACTGGCGGTAGGGGATATCGTAAACATTGAGGTTGATGTGATCGCTAA
GTACGTCGAACGCATGATGACGCGCGGCGTGGCTGGAAACACTCCCAATGACTACACCGATTTGACGAGAGACTAG

Upstream 100 bases:

>100_bases
ACAAGAAACCACAATGGATCAGATTATGCGTTTTGACACCACGTCCGTGAGACAGTTGGGTTCAGATGTATTGATAGAAA
TGATGAGAAAGGAACACTAA

Downstream 100 bases:

>100_bases
GTTAGACAACGTGAGTGAACATGAGCAGGCACACAGCCAATTAGATTCTGTTGAAGAGGCCATCGCTGACATCGCTGCGG
GTAAAGCCGTCGTGGTGGTA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFTGIVEELGSVAGVEHLGDSIRMQISASTVLEGVHLGDSISVNGVCLTVASFDEGHFTADLMQETLDRSSLGALSTGSK
VNLERAMAADGRLGGHIMQGHVDATTTLIKRTSSENWDVLRFELPADLARYVVEKGSIALNGTSLTVSSLGDDWFEVSLI
PTTLRDTTHGELAVGDIVNIEVDVIAKYVERMMTRGVAGNTPNDYTDLTRD

Sequences:

>Translated_211_residues
MFTGIVEELGSVAGVEHLGDSIRMQISASTVLEGVHLGDSISVNGVCLTVASFDEGHFTADLMQETLDRSSLGALSTGSK
VNLERAMAADGRLGGHIMQGHVDATTTLIKRTSSENWDVLRFELPADLARYVVEKGSIALNGTSLTVSSLGDDWFEVSLI
PTTLRDTTHGELAVGDIVNIEVDVIAKYVERMMTRGVAGNTPNDYTDLTRD
>Mature_211_residues
MFTGIVEELGSVAGVEHLGDSIRMQISASTVLEGVHLGDSISVNGVCLTVASFDEGHFTADLMQETLDRSSLGALSTGSK
VNLERAMAADGRLGGHIMQGHVDATTTLIKRTSSENWDVLRFELPADLARYVVEKGSIALNGTSLTVSSLGDDWFEVSLI
PTTLRDTTHGELAVGDIVNIEVDVIAKYVERMMTRGVAGNTPNDYTDLTRD

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=210, Percent_Identity=37.1428571428571, Blast_Score=119, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=38.5365853658537, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22600; Mature: 22600

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEELGSVAGVEHLGDSIRMQISASTVLEGVHLGDSISVNGVCLTVASFDEGHFTA
CCCHHHHHHHHHHCHHHCCCCEEEEEEHHHHHHHEECCCCEEECEEEEEEEECCCCCEEH
DLMQETLDRSSLGALSTGSKVNLERAMAADGRLGGHIMQGHVDATTTLIKRTSSENWDVL
HHHHHHHCHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEE
RFELPADLARYVVEKGSIALNGTSLTVSSLGDDWFEVSLIPTTLRDTTHGELAVGDIVNI
EEECCHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEEEEEECCCCCCCCEEECCEEEE
EVDVIAKYVERMMTRGVAGNTPNDYTDLTRD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFTGIVEELGSVAGVEHLGDSIRMQISASTVLEGVHLGDSISVNGVCLTVASFDEGHFTA
CCCHHHHHHHHHHCHHHCCCCEEEEEEHHHHHHHEECCCCEEECEEEEEEEECCCCCEEH
DLMQETLDRSSLGALSTGSKVNLERAMAADGRLGGHIMQGHVDATTTLIKRTSSENWDVL
HHHHHHHCHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEE
RFELPADLARYVVEKGSIALNGTSLTVSSLGDDWFEVSLIPTTLRDTTHGELAVGDIVNI
EEECCHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEEEEEECCCCCCCCEEECCEEEE
EVDVIAKYVERMMTRGVAGNTPNDYTDLTRD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]