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The map label for this gene is aspA [H]

Identifier: 145295636

GI number: 145295636

Start: 1728408

End: 1729988

Strand: Reverse

Name: aspA [H]

Synonym: cgR_1563

Alternate gene names: 145295636

Gene position: 1729988-1728408 (Counterclockwise)

Preceding gene: 145295637

Following gene: 145295635

Centisome position: 52.2

GC content: 53.13

Gene sequence:

>1581_bases
ATGTCTAAGACGAGCAACAAGTCTTCAGCAGACTCAAAGAATGACGCAAAAGCCGAAGACATTGTGAACGGCGAGAACCA
AATCGCCACGAATGAGTCGCAGTCTTCAGACAGCGCTGCAGTTTCGGAACGTGTCGTCGAACCAAAAACCACGGTTCAGA
AAAAGTTCCGAATCGAATCGGATCTGCTTGGTGAACTTCAGATCCCATCCCACGCATATTACGGCGTGCACACCCTTCGT
GCGGTGGACAACTTCCAAATCTCACGAACCACCATCAACCACGTCCCAGATTTCATTCGCGGCATGGTCCAGGTGAAAAA
GGCCGCAGCTTTAGCAAACCGCCGACTACACACACTTCCAGCACAAAAAGCAGAAGCAATTGTCTGGGCTTGTGATCAGA
TCCTCATTGAGGGACGCTGTATGGATCAGTTCCCCATCGATGTGTTCCAGGGTGGCGCAGGTACCTCACTGAACATGAAC
ACCAACGAGGTTGTTGCCAACCTTGCACTTGAGTTCTTAGGCCATGAAAAGGGCGAGTACCACATCCTGCACCCCATGGA
TGATGTGAACATGTCCCAGTCCACCAACGATTCCTACCCAACTGGTTTCCGCCTGGGCATTTACGCTGGACTGCAGACCC
TCATCGCTGAAATTGATGAGCTTCAGGTTGCGTTCCGCCACAAGGGCAATGAGTTTGTCGACATCATCAAGATGGGCCGC
ACCCAGTTGCAGGATGCTGTTCCCATGAGCTTGGGCGAAGAGTTCCGAGCATTCGCGCACAACCTCGCAGAAGAGCAGAC
CGTGCTGCGTGAAGCTGCCAACCGTCTCCTCGAGGTCAACCTTGGTGCAACCGCAATCGGTACTGGTGTGAACACTCCAG
CAGGCTACCGCCACCAGGTTGTCGCTGCTCTGTCTGAGGTCACCGGACTGGAACTAAAGTCCGCACGTGATCTCATTGAG
GCTACCTCTGACACCGGTGCATATGTTCATGCGCACTCCGCAATCAAGCGTGCAGCCATGAAACTGTCCAAGATCTGTAA
CGATCTACGTCTGCTGTCTTCTGGTCCTCGTGCTGGCTTGAACGAAATCAATCTGCCACCACGCCAGGCTGGTTCCTCCA
TCATGCCAGCCAAGGTCAACCCAGTGATCCCAGAAGTGGTCAACCAGGTCTGCTTCAAGGTCTTCGGTAACGATCTCACC
GTCACCATGGCTGCGGAAGCTGGCCAGTTGCAGCTCAACGTCATGGAGCCAGTCATTGGCGAATCCCTCTTCCAGTCACT
GCGCATCCTGGGCAATGCAGCCAAGACTTTGCGTGAGAAGTGCGTCGTAGGAATCACCGCCAACGCTGATGTTTGCCGTG
CTTACGTTGATAACTCCATTGGAATTATCACTTACCTGAACCCATTCCTGGGCCACGACATTGGAGATCAGATCGGTAAG
GAAGCAGCCGAAACTGGTCGACCAGTGCGTGAACTCATCCTGGAAAAGAAGCTCATGGATGAAAAGACGCTCGAGGCAGT
CCTATCCAAGGAGAACCTCATGCACCCAATGTTCCGCGGAAGGCTCTACTTGGAGAACTAA

Upstream 100 bases:

>100_bases
CCCCGAAAATGTAGTGGGGTAGTTCCACACTGCCTCTTACAAGTACGTAGGATAATCCACAGCACCATCGTGATTTCCTT
CAACTTGTGAGAGGCAGTAC

Downstream 100 bases:

>100_bases
TCCAAGATCTCGTCTGATACCTGGGCAATTAGGTTGAGAGTTCATCTCACCTGGTTGCCCAGGTTTTTTCGTGGGTGAAT
TCAGCAGGCGCCTGTTTAAG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLR
AVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEGRCMDQFPIDVFQGGAGTSLNMN
TNEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIE
ATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLT
VTMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN

Sequences:

>Translated_526_residues
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLR
AVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEGRCMDQFPIDVFQGGAGTSLNMN
TNEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIE
ATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLT
VTMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
>Mature_525_residues
SKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLRA
VDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEGRCMDQFPIDVFQGGAGTSLNMNT
NEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGRT
QLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIEA
TSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTV
TMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGKE
AAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=38.0645161290323, Blast_Score=321, Evalue=1e-87,
Organism=Escherichia coli, GI87082375, Length=468, Percent_Identity=64.5299145299145, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1787896, Length=470, Percent_Identity=36.5957446808511, Blast_Score=302, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17553882, Length=470, Percent_Identity=38.2978723404255, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=37.3088685015291, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=36.7965367965368, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=37.9310344827586, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=37.9310344827586, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=37.9609544468547, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24662684, Length=470, Percent_Identity=35.9574468085106, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24583245, Length=456, Percent_Identity=35.0877192982456, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 57497; Mature: 57366

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIES
CCCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHCCH
DLLGELQIPSHAYYGVHTLRAVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLP
HHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
AQKAEAIVWACDQILIEGRCMDQFPIDVFQGGAGTSLNMNTNEVVANLALEFLGHEKGEY
CHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
HILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHH
VAALSEVTGLELKSARDLIEATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGL
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIG
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHH
ESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
HHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEHHCHHHCCCHHHHHHH
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure 
SKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIES
CCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHCCH
DLLGELQIPSHAYYGVHTLRAVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLP
HHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
AQKAEAIVWACDQILIEGRCMDQFPIDVFQGGAGTSLNMNTNEVVANLALEFLGHEKGEY
CHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
HILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHH
VAALSEVTGLELKSARDLIEATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGL
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIG
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHH
ESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
HHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEHHCHHHCCCHHHHHHH
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7789816; 12948626 [H]