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The map label for this gene is dop [H]

Identifier: 145295629

GI number: 145295629

Start: 1719901

End: 1721445

Strand: Reverse

Name: dop [H]

Synonym: cgR_1556

Alternate gene names: 145295629

Gene position: 1721445-1719901 (Counterclockwise)

Preceding gene: 145295630

Following gene: 145295628

Centisome position: 51.94

GC content: 54.82

Gene sequence:

>1545_bases
ATGGCGCGTTTTATGGGATCGGAAACCGAATACGGCATTTCCACACCGTCTGCACCGTTTTTAAGCCCGATCGTTACTTC
CACGCACGCGGTGGTGGCGTATTCCACCGCGCGTGGATTTGGTGAGCACCGGGTGCGTTGGGACTATGCGCAAGAGTCCC
CACTGCGCGATACTCGTGGCTTTGATCTGCGTCGATACCACCAGGCCCCTGTGGTGGATCCGCACGCCATTGGTGTGGCC
AACGTGTTTGCGCCCAATGGTGCCAGGTTTTATGTCGATCACGCGCACCCGGAATACTCCTCCCCAGAGGTCACCAATGC
GTGGGATGCCATGGTTTACGATGCCGCCGGTGATCACATCCTCATGCAGGCCGTCTCTGATGTTGCGAGTTTCACCAGCC
AGAATAGGTCTGTGTTGGACGGCCATGATCCGTGCCCGGCTTTGAAAATCTACAAAAACAATGTCGACGGTAAGGGTGCT
AGCTACGGCTCCCACGAGAATTACCTTTACTCACGTGAGACCGATTTTGATGTGCTGGCTCAGGCATTGATCCCATTTTT
TGTGTGTCGCCAGGTCATCATCGGTGCCGGACGCGTGGGATTGGGTCCTCAGTGCGATAAGCCCGGATTCCAGATCAGCC
AGCGAGCTGACTACATTGAGCAAGAAATTTCACTGGAAACCACACTCAACCGCGGCATTATCAACACCCGCGATGAACCA
CACACCGACGCTGATCACTGGGGTCGCCTGCACGTGATCATCGGCGATGCCAACATGTCGCAGACTGCGAATTTCCTCAA
ATTCGGCATGACCTCCCTGGTGCTGGATGCCATTGAGGCTGGGGTGGATTTCTCTGAACTCAAGCTGAAGAACGCGGTGA
GTGAAGTAGCAAAGGTCTCCCATGATCTTTCCCTTACCCACCAGCTGCAATTGGCGGATGGTTCAGAGCTCACCGCTATT
GATATTCTGCGCCGCTACTTGGACAAGGTGCAGCCGTTTGCAGAAACCCCAGCGGAACAACGCGTCACTGCGCTGTGGGG
TGAGGTGCTGGGGCTCCTGGAGAATGATCCGCTCTCCACCAGCCATCTCCTTGATTGGACTGCAAAACTTGCCCTGATCA
AGTCTTTTGAGGCGCGTGGGCTGTCCATTAACGATCCCAAGATGTTCCTCATTGACCTGCAGTACAGCGATATTGATCCG
CAGAAGAGCCTGTATCACGCCCTGGTATCCAAGGGGCGGATGAAAACACTGTGCAGTGCGCAGGACATTGCAGATGCAGC
GGCCACTCCACCGCAGGACTCGCGGGCATACTTCCGCGGACGTGTGATGGATAAATTTGGCGAATCAGTGCTCGCAGCAA
ACTGGGAATCGCTGATTGTTGCTACCTCCAGAGCTAAGGAATACCGCGTGCACACCAGCGGTTTGGATGCCTTGACCCGA
GAAAAAGCAGGGGAGTTGGTGGAGAAATCCGCCTCGATTGATGAGTTAATTGAGGGGCTTGAAAAGGAAAACTTGACCCC
GCTCACGGCCATTCAAGCTATTTAG

Upstream 100 bases:

>100_bases
GCAGAGCGAAGACCTGCCAAACACCTCCAACCCGGATGAATGGTCCAGGATCACTGGCCGCCAGGGCAAGCAGGTTACTC
ACGCAGAGGTGGTTATCTAA

Downstream 100 bases:

>100_bases
GGTACCCTGAATAGGTGGTTAGGGGATGTCCCTAGAATCATCTCAGGGGTGAATCAAATCCCTTCAACTAACCCATGTGA
CGGCGCTAACGTGTGATGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MARFMGSETEYGISTPSAPFLSPIVTSTHAVVAYSTARGFGEHRVRWDYAQESPLRDTRGFDLRRYHQAPVVDPHAIGVA
NVFAPNGARFYVDHAHPEYSSPEVTNAWDAMVYDAAGDHILMQAVSDVASFTSQNRSVLDGHDPCPALKIYKNNVDGKGA
SYGSHENYLYSRETDFDVLAQALIPFFVCRQVIIGAGRVGLGPQCDKPGFQISQRADYIEQEISLETTLNRGIINTRDEP
HTDADHWGRLHVIIGDANMSQTANFLKFGMTSLVLDAIEAGVDFSELKLKNAVSEVAKVSHDLSLTHQLQLADGSELTAI
DILRRYLDKVQPFAETPAEQRVTALWGEVLGLLENDPLSTSHLLDWTAKLALIKSFEARGLSINDPKMFLIDLQYSDIDP
QKSLYHALVSKGRMKTLCSAQDIADAAATPPQDSRAYFRGRVMDKFGESVLAANWESLIVATSRAKEYRVHTSGLDALTR
EKAGELVEKSASIDELIEGLEKENLTPLTAIQAI

Sequences:

>Translated_514_residues
MARFMGSETEYGISTPSAPFLSPIVTSTHAVVAYSTARGFGEHRVRWDYAQESPLRDTRGFDLRRYHQAPVVDPHAIGVA
NVFAPNGARFYVDHAHPEYSSPEVTNAWDAMVYDAAGDHILMQAVSDVASFTSQNRSVLDGHDPCPALKIYKNNVDGKGA
SYGSHENYLYSRETDFDVLAQALIPFFVCRQVIIGAGRVGLGPQCDKPGFQISQRADYIEQEISLETTLNRGIINTRDEP
HTDADHWGRLHVIIGDANMSQTANFLKFGMTSLVLDAIEAGVDFSELKLKNAVSEVAKVSHDLSLTHQLQLADGSELTAI
DILRRYLDKVQPFAETPAEQRVTALWGEVLGLLENDPLSTSHLLDWTAKLALIKSFEARGLSINDPKMFLIDLQYSDIDP
QKSLYHALVSKGRMKTLCSAQDIADAAATPPQDSRAYFRGRVMDKFGESVLAANWESLIVATSRAKEYRVHTSGLDALTR
EKAGELVEKSASIDELIEGLEKENLTPLTAIQAI
>Mature_513_residues
ARFMGSETEYGISTPSAPFLSPIVTSTHAVVAYSTARGFGEHRVRWDYAQESPLRDTRGFDLRRYHQAPVVDPHAIGVAN
VFAPNGARFYVDHAHPEYSSPEVTNAWDAMVYDAAGDHILMQAVSDVASFTSQNRSVLDGHDPCPALKIYKNNVDGKGAS
YGSHENYLYSRETDFDVLAQALIPFFVCRQVIIGAGRVGLGPQCDKPGFQISQRADYIEQEISLETTLNRGIINTRDEPH
TDADHWGRLHVIIGDANMSQTANFLKFGMTSLVLDAIEAGVDFSELKLKNAVSEVAKVSHDLSLTHQLQLADGSELTAID
ILRRYLDKVQPFAETPAEQRVTALWGEVLGLLENDPLSTSHLLDWTAKLALIKSFEARGLSINDPKMFLIDLQYSDIDPQ
KSLYHALVSKGRMKTLCSAQDIADAAATPPQDSRAYFRGRVMDKFGESVLAANWESLIVATSRAKEYRVHTSGLDALTRE
KAGELVEKSASIDELIEGLEKENLTPLTAIQAI

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 56698; Mature: 56567

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFMGSETEYGISTPSAPFLSPIVTSTHAVVAYSTARGFGEHRVRWDYAQESPLRDTRG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHCCCCCCCEEECCCCCCCCCCCCC
FDLRRYHQAPVVDPHAIGVANVFAPNGARFYVDHAHPEYSSPEVTNAWDAMVYDAAGDHI
CCHHHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEECCCCCHH
LMQAVSDVASFTSQNRSVLDGHDPCPALKIYKNNVDGKGASYGSHENYLYSRETDFDVLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHH
QALIPFFVCRQVIIGAGRVGLGPQCDKPGFQISQRADYIEQEISLETTLNRGIINTRDEP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HTDADHWGRLHVIIGDANMSQTANFLKFGMTSLVLDAIEAGVDFSELKLKNAVSEVAKVS
CCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HDLSLTHQLQLADGSELTAIDILRRYLDKVQPFAETPAEQRVTALWGEVLGLLENDPLST
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
SHLLDWTAKLALIKSFEARGLSINDPKMFLIDLQYSDIDPQKSLYHALVSKGRMKTLCSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
QDIADAAATPPQDSRAYFRGRVMDKFGESVLAANWESLIVATSRAKEYRVHTSGLDALTR
HHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHEEEEECCCHHEEEECCCHHHHHH
EKAGELVEKSASIDELIEGLEKENLTPLTAIQAI
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure 
ARFMGSETEYGISTPSAPFLSPIVTSTHAVVAYSTARGFGEHRVRWDYAQESPLRDTRG
CCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHCCCCCCCEEECCCCCCCCCCCCC
FDLRRYHQAPVVDPHAIGVANVFAPNGARFYVDHAHPEYSSPEVTNAWDAMVYDAAGDHI
CCHHHHCCCCCCCCHHCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEECCCCCHH
LMQAVSDVASFTSQNRSVLDGHDPCPALKIYKNNVDGKGASYGSHENYLYSRETDFDVLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHH
QALIPFFVCRQVIIGAGRVGLGPQCDKPGFQISQRADYIEQEISLETTLNRGIINTRDEP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HTDADHWGRLHVIIGDANMSQTANFLKFGMTSLVLDAIEAGVDFSELKLKNAVSEVAKVS
CCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HDLSLTHQLQLADGSELTAIDILRRYLDKVQPFAETPAEQRVTALWGEVLGLLENDPLST
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
SHLLDWTAKLALIKSFEARGLSINDPKMFLIDLQYSDIDPQKSLYHALVSKGRMKTLCSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
QDIADAAATPPQDSRAYFRGRVMDKFGESVLAANWESLIVATSRAKEYRVHTSGLDALTR
HHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHEEEEECCCHHEEEECCCHHHHHH
EKAGELVEKSASIDELIEGLEKENLTPLTAIQAI
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7583123 [H]