Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 145295540

GI number: 145295540

Start: 1620927

End: 1622189

Strand: Direct

Name: tyrS [H]

Synonym: cgR_1468

Alternate gene names: 145295540

Gene position: 1620927-1622189 (Clockwise)

Preceding gene: 145295539

Following gene: 145295543

Centisome position: 48.91

GC content: 57.32

Gene sequence:

>1263_bases
ATGAACATCATTGACGAACTCTCCTGGCGCGGACTCATCAACCAGTCCACCGACCTTGAGGCCCTTCGCGAGGAAGCTTC
AACCCCCATCACCTTGTACTGCGGATTCGACCCAACCGGTCCATCCCTCCACGCAGGACACCTCGTTCCACTGCTCATGC
TGCGCCGTTTCCAGCAAGCAGGACACAACCCAATCGTGCTTGCAGGTGGCGCAACCGGCATGATCGGCGACCCCCGCGAT
GTCGGCGAGCGCACCATGAACTCCGCAGACACCGTCTCTGTTTGGGCAGAACGCATCTCCGGTCAGCTCAGCCGCTTCGT
TGATTTCGACGGCGAGCACGCAGCCCGTCTGGTCAACAACGCCGAGTGGACCAACGAAATGTCCGTAGTGACCTTTCTCC
GTGATGTAGGAAAGCACTTCTCCCTCAACACCATGCTCGCTCGCGACACCGTCAAGCGTCGCCTCGAGTCCGACGGCATT
TCCTACACCGAGTTCTCCTACATGCTGCTGCAGGCCAACGACTACGTTGAACTGAACAAGCGCTTCGGCTGCACCCTGCA
GGTAGGTGGCGGTGACCAGTGGGGCAACATCGTTTCTGGCGTTGACCTCAACCGTCGCGTCAACGGCACCTCTGTCCACG
CAGTGACCGTACCTTTGGTTACCGATTCCGACGGCAAGAAGTTCGGCAAGTCCACCGGCGGCGGAAGTCTCTGGTTGGAT
CCGGAAATGACCAGCCCATACGCTTGGTACCAGTACTTCATCAACGCATCCGACGCTGACGTGATCCGTTACCTCCGCTG
GTTCACCTTCCTCACCCAGGAAGAACTTGCTGAGTTGGAAGTTGAAGTTGCAGAGCGCCCATTCAAGCGCGAAGCACAGC
GTCGTCTTGCACGCGAAATGACCAACTTGGTTCACGGAACCGAAGCAACCGAAGCAGTCGAGCTTGCTGCTCAAGCACTG
TTCGGCCGCGCAGAACTCCGCGACTTAGACGAAAAAACTCTCGCTGCATCCGTTTCTGAAACTGCAGTTGCAGAAATCAA
GGCAGGGGAGCCACGCACCATCATAGATCTGTTGGTGGCCAGCGGACTTGCTGATTCCAAGGGCGCAGCAAAGCGTGCAG
TCAAGGAAGGCGGTGCGTACGTAAACAACGAACGTATCGAATCGGATGATTGGGAGCCTTCCGCTGAAGACCTTCTTCAC
GGTTCATGGCTAGTACTGCGCCGTGGCAAGAAGAACTTCGCAGGAGTTCAGATCCTCGGCTAA

Upstream 100 bases:

>100_bases
GGCCTATCGCATTGACGACGGAATTCCGGTGCTTCTCATCGACGAAGCCACCGAGTGGACCCCCAACAACTAGAAGTACA
TCAAGGACATTTTTAACACC

Downstream 100 bases:

>100_bases
TAATCGCTGAACTGATTGCGGTTTTTCATTTGTCTGACTTCTGGAAAACCAAAAGGAGGGGTGAGCACCAAATTCTGGTG
CCCACCCCTCCTTTTAGCAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNIIDELSWRGLINQSTDLEALREEASTPITLYCGFDPTGPSLHAGHLVPLLMLRRFQQAGHNPIVLAGGATGMIGDPRD
VGERTMNSADTVSVWAERISGQLSRFVDFDGEHAARLVNNAEWTNEMSVVTFLRDVGKHFSLNTMLARDTVKRRLESDGI
SYTEFSYMLLQANDYVELNKRFGCTLQVGGGDQWGNIVSGVDLNRRVNGTSVHAVTVPLVTDSDGKKFGKSTGGGSLWLD
PEMTSPYAWYQYFINASDADVIRYLRWFTFLTQEELAELEVEVAERPFKREAQRRLAREMTNLVHGTEATEAVELAAQAL
FGRAELRDLDEKTLAASVSETAVAEIKAGEPRTIIDLLVASGLADSKGAAKRAVKEGGAYVNNERIESDDWEPSAEDLLH
GSWLVLRRGKKNFAGVQILG

Sequences:

>Translated_420_residues
MNIIDELSWRGLINQSTDLEALREEASTPITLYCGFDPTGPSLHAGHLVPLLMLRRFQQAGHNPIVLAGGATGMIGDPRD
VGERTMNSADTVSVWAERISGQLSRFVDFDGEHAARLVNNAEWTNEMSVVTFLRDVGKHFSLNTMLARDTVKRRLESDGI
SYTEFSYMLLQANDYVELNKRFGCTLQVGGGDQWGNIVSGVDLNRRVNGTSVHAVTVPLVTDSDGKKFGKSTGGGSLWLD
PEMTSPYAWYQYFINASDADVIRYLRWFTFLTQEELAELEVEVAERPFKREAQRRLAREMTNLVHGTEATEAVELAAQAL
FGRAELRDLDEKTLAASVSETAVAEIKAGEPRTIIDLLVASGLADSKGAAKRAVKEGGAYVNNERIESDDWEPSAEDLLH
GSWLVLRRGKKNFAGVQILG
>Mature_420_residues
MNIIDELSWRGLINQSTDLEALREEASTPITLYCGFDPTGPSLHAGHLVPLLMLRRFQQAGHNPIVLAGGATGMIGDPRD
VGERTMNSADTVSVWAERISGQLSRFVDFDGEHAARLVNNAEWTNEMSVVTFLRDVGKHFSLNTMLARDTVKRRLESDGI
SYTEFSYMLLQANDYVELNKRFGCTLQVGGGDQWGNIVSGVDLNRRVNGTSVHAVTVPLVTDSDGKKFGKSTGGGSLWLD
PEMTSPYAWYQYFINASDADVIRYLRWFTFLTQEELAELEVEVAERPFKREAQRRLAREMTNLVHGTEATEAVELAAQAL
FGRAELRDLDEKTLAASVSETAVAEIKAGEPRTIIDLLVASGLADSKGAAKRAVKEGGAYVNNERIESDDWEPSAEDLLH
GSWLVLRRGKKNFAGVQILG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=35.3658536585366, Blast_Score=232, Evalue=4e-61,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=47.8468899521531, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25145802, Length=422, Percent_Identity=33.8862559241706, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25145805, Length=296, Percent_Identity=34.4594594594595, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325160, Length=448, Percent_Identity=34.1517857142857, Blast_Score=250, Evalue=4e-67,
Organism=Drosophila melanogaster, GI20130383, Length=405, Percent_Identity=35.5555555555556, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46370; Mature: 46370

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDELSWRGLINQSTDLEALREEASTPITLYCGFDPTGPSLHAGHLVPLLMLRRFQQA
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GHNPIVLAGGATGMIGDPRDVGERTMNSADTVSVWAERISGQLSRFVDFDGEHAARLVNN
CCCCEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
AEWTNEMSVVTFLRDVGKHFSLNTMLARDTVKRRLESDGISYTEFSYMLLQANDYVELNK
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHEEEEEEECCCCEEECC
RFGCTLQVGGGDQWGNIVSGVDLNRRVNGTSVHAVTVPLVTDSDGKKFGKSTGGGSLWLD
CCCCEEEECCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCHHHCCCCCCCCEEEC
PEMTSPYAWYQYFINASDADVIRYLRWFTFLTQEELAELEVEVAERPFKREAQRRLAREM
CCCCCCCEEHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TNLVHGTEATEAVELAAQALFGRAELRDLDEKTLAASVSETAVAEIKAGEPRTIIDLLVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SGLADSKGAAKRAVKEGGAYVNNERIESDDWEPSAEDLLHGSWLVLRRGKKNFAGVQILG
CCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCEEEEC
>Mature Secondary Structure
MNIIDELSWRGLINQSTDLEALREEASTPITLYCGFDPTGPSLHAGHLVPLLMLRRFQQA
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GHNPIVLAGGATGMIGDPRDVGERTMNSADTVSVWAERISGQLSRFVDFDGEHAARLVNN
CCCCEEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
AEWTNEMSVVTFLRDVGKHFSLNTMLARDTVKRRLESDGISYTEFSYMLLQANDYVELNK
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHEEEEEEECCCCEEECC
RFGCTLQVGGGDQWGNIVSGVDLNRRVNGTSVHAVTVPLVTDSDGKKFGKSTGGGSLWLD
CCCCEEEECCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCHHHCCCCCCCCEEEC
PEMTSPYAWYQYFINASDADVIRYLRWFTFLTQEELAELEVEVAERPFKREAQRRLAREM
CCCCCCCEEHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TNLVHGTEATEAVELAAQALFGRAELRDLDEKTLAASVSETAVAEIKAGEPRTIIDLLVA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SGLADSKGAAKRAVKEGGAYVNNERIESDDWEPSAEDLLHGSWLVLRRGKKNFAGVQILG
CCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]