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The map label for this gene is argF [H]

Identifier: 145295533

GI number: 145295533

Start: 1614498

End: 1615457

Strand: Direct

Name: argF [H]

Synonym: cgR_1461

Alternate gene names: 145295533

Gene position: 1614498-1615457 (Clockwise)

Preceding gene: 145295532

Following gene: 145295534

Centisome position: 48.71

GC content: 56.77

Gene sequence:

>960_bases
ATGACTTCACAACCACAGGTTCGCCATTTCCTGGCTGATGATGATCTCACCCCTGCAGAGCAGGCTGAGGTTTTGACCCT
GGCCGCAAAGCTTAAGGCAGCGCCGTTTTCGGAGCGTCCACTCGAGGGACCAAAGTCCGTTGCAGTTCTTTTTGATAAGA
CTTCAACTCGTACTCGCTTCTCCTTCGACGCGGGCATCGCTCATTTGGGTGGACACGCCATCGTCGTGGATTCCGGCAGC
TCACAGATGGGTAAGGGCGAGACCCTGCAGGACACCGCAGCTGTATTGTCCCGCTACGTGGAAGCAATTGTGTGGCGCAC
CTACGCACACAGCAATTTCCACGCCATGGCGGAGACGTCCACTGTGCCGCTGGTGAACTCCTTGACCGATGATCTGCACC
CATGCCAGATTCTGGCTGATCTGCAGACCATCGTGGAAAACCTCAGCCCTGAAGAAGGCCCAGCAGGCCTTAAGGGTAAG
AAGGCTGTGTACCTGGGCGATGGCGACAACAACATGGCCAACTCCTACATGATTGGCTTTGCCACCGCGGGCATGGATAT
TTCCATCATCGCTCCTGAAGGGTTCCAGCCTCGTGCGGAATTCGTGGAGCGCGCGGAAAAGCGTGGCCAGGAAACCGGCG
CGAAGGTTGTTGTCACCGACAGTCTCGACGAGGTTGCCGGCGCCGATGTTGTCATCACCGATACCTGGGTATCCATGGGT
ATGGAAAACGACGGCATCGATCGCACCACACCTTTCGTTCCTTACCAGGTCAACGATGAGGTCATGGCGAAAGCTAACGA
CGGCGCCATCTTCCTGCACTGCCTTCCTGCCTACCGTGGCAAAGAAGTGGCAGCCTCCGTGATTGATGGACCAGCGTCCA
AAGTTTTCGATGAAGCAGAAAACCGCCTCCACGCTCAGAAAGCACTGCTGGTGTGGCTGCTGGCCAACCAGCCGAGGTAA

Upstream 100 bases:

>100_bases
AACATTATCCGTTTGACGCCGCCGCTGGTGATCACCGACGAAGAAATCGCAGACGCAGTCAAGGCTATTGCCGAGACAAT
CGCATAAAGGACTTAAACTT

Downstream 100 bases:

>100_bases
GACATGTCTCTTGGCTCAACCCCGTCGACACCGGAAAACTTAAATCCCGTGACTCGCACTGCACGCCAAGCTCTCATTTT
GCAGATTTTGGACAAACAAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGS
SQMGKGETLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLTDDLHPCQILADLQTIVENLSPEEGPAGLKGK
KAVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR

Sequences:

>Translated_319_residues
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGS
SQMGKGETLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLTDDLHPCQILADLQTIVENLSPEEGPAGLKGK
KAVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR
>Mature_318_residues
TSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRFSFDAGIAHLGGHAIVVDSGSS
QMGKGETLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETSTVPLVNSLTDDLHPCQILADLQTIVENLSPEEGPAGLKGKK
AVYLGDGDNNMANSYMIGFATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMGM
ENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAENRLHAQKALLVWLLANQPR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=317, Percent_Identity=37.8548895899054, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI18105007, Length=318, Percent_Identity=23.8993710691824, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790703, Length=333, Percent_Identity=37.5375375375375, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=35.1681957186544, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI48994908, Length=323, Percent_Identity=28.1733746130031, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=30.0940438871473, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=277, Percent_Identity=35.0180505415162, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=26.0450160771704, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=26.8987341772152, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34357; Mature: 34226

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRF
CCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
SFDAGIAHLGGHAIVVDSGSSQMGKGETLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETS
EECCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TVPLVNSLTDDLHPCQILADLQTIVENLSPEEGPAGLKGKKAVYLGDGDNNMANSYMIGF
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
ATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
EECCCEEEEECCCCCCCHHHHHHHHHHCCHHCCCEEEEECCHHHHCCCCEEEEEHHHHCC
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAE
CCCCCCCCCCCCCEEEECCHHEEECCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
NRLHAQKALLVWLLANQPR
HHHHHHHHHEEEEECCCCC
>Mature Secondary Structure 
TSQPQVRHFLADDDLTPAEQAEVLTLAAKLKAAPFSERPLEGPKSVAVLFDKTSTRTRF
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEE
SFDAGIAHLGGHAIVVDSGSSQMGKGETLQDTAAVLSRYVEAIVWRTYAHSNFHAMAETS
EECCCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
TVPLVNSLTDDLHPCQILADLQTIVENLSPEEGPAGLKGKKAVYLGDGDNNMANSYMIGF
CCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEE
ATAGMDISIIAPEGFQPRAEFVERAEKRGQETGAKVVVTDSLDEVAGADVVITDTWVSMG
EECCCEEEEECCCCCCCHHHHHHHHHHCCHHCCCEEEEECCHHHHCCCCEEEEEHHHHCC
MENDGIDRTTPFVPYQVNDEVMAKANDGAIFLHCLPAYRGKEVAASVIDGPASKVFDEAE
CCCCCCCCCCCCCEEEECCHHEEECCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHH
NRLHAQKALLVWLLANQPR
HHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10420995; 12948626; 8581175 [H]