Click here to switch to the map view.

The map label for this gene is ysgA [H]

Identifier: 145295523

GI number: 145295523

Start: 1602070

End: 1602885

Strand: Direct

Name: ysgA [H]

Synonym: cgR_1451

Alternate gene names: 145295523

Gene position: 1602070-1602885 (Clockwise)

Preceding gene: 145295521

Following gene: 145295524

Centisome position: 48.34

GC content: 56.86

Gene sequence:

>816_bases
ATGGCATTGGACTTTAATGAGGCGTTTACCGAACGCACCCCGCGCATCGTCAACGCAGCTAAACTGCATCGCGCCGCGCA
GCGCAAAAAAGATAAGCGTTTTCTAGTTGAAGGCGAAAATTCCGTTGAAGCAGCTGTCGCTACCGGCGCAGCAACTGACC
TCTTTGTCACTGAATCCGCTGCGGAGCGCTTCGAAGAAATCGTCCGCACCGCCGGTTACATGAATGTCTACACCCACGCG
ATCACGGACAAGGCTGCAAAGCATCTTAGCGACACCGTCACCACCACGGGCATTTTTGCGCTTTGCGACGACGTCCTGTG
GTCAGTCGGCAAGGCGATCACCGGCCAGCCACGTCTAGTGAGCGTGCCGGTTGAGACCCGCGAGCCCGGTAATGCCGGAA
CCTTGATTCGCGTATCCGACGCGGTCGGCGCCGACGCCGTCGTCTTCGCTGGTGAATCAGTAGATCCACTTGGCGCAAAA
GCTGTGCGCTCCTCAGCGGGATCGCTGTTTCACATTCCAGTGGCACGCAACAACAACATCGCAGATGTCTTGGGGCAGCT
TCGTTCCAAGGGCCTGCAGATCCTTGCGACCTCAGCCGATGGGGAAGTAGACCTCGATGACGCCGATGAGCTGCTAGCCA
AGCCAACCGCATGGCTTTTTGGTAATGAAGCTCACGGACTTGATGAGAGTCTGCTTGCTCAGGCTGATCACCGCGTGCGT
ATTCCGATCCGCGGCCGCGCAGAATCACTCAATTTGGCCACAGCAGCGTCAATTTGTTTGTACGAATCCTCCAAGGCACT
ATTCGCCGGTGAGTAA

Upstream 100 bases:

>100_bases
GATGGGCAACAATGCGCATATTCTTATACATCCTTTTTTGAGGGCTTTTTAAGGATAGGGAGCAAAGTTGACCGCACGGT
TAACTGTTAGTGTTATGAGC

Downstream 100 bases:

>100_bases
ACCCCAATTCATGCCCAGCGGGTATGATGGCGAGGTTAGTTGACTTAACAATAAACATGAGGGAAGGGCTGGACTACACG
GTGTCCGAAATTCAGTTGAC

Product: hypothetical protein

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVRTAGYMNVYTHA
ITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAK
AVRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDESLLAQADHRVR
IPIRGRAESLNLATAASICLYESSKALFAGE

Sequences:

>Translated_271_residues
MALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVRTAGYMNVYTHA
ITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAK
AVRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDESLLAQADHRVR
IPIRGRAESLNLATAASICLYESSKALFAGE
>Mature_270_residues
ALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESAAERFEEIVRTAGYMNVYTHAI
TDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLVSVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAKA
VRSSAGSLFHIPVARNNNIADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDESLLAQADHRVRI
PIRGRAESLNLATAASICLYESSKALFAGE

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790623, Length=138, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 28813; Mature: 28681

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESA
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHEEEEHHH
AERFEEIVRTAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAKAVRSSAGSLFHIPVARNNNI
EECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCH
ADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDESLLAQADHRVR
HHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCHHHHHCCCCEEE
IPIRGRAESLNLATAASICLYESSKALFAGE
EEECCCCCCCCHHHHHEEEEEECCCCEECCC
>Mature Secondary Structure 
ALDFNEAFTERTPRIVNAAKLHRAAQRKKDKRFLVEGENSVEAAVATGAATDLFVTESA
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCHHEEEEHHH
AERFEEIVRTAGYMNVYTHAITDKAAKHLSDTVTTTGIFALCDDVLWSVGKAITGQPRLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SVPVETREPGNAGTLIRVSDAVGADAVVFAGESVDPLGAKAVRSSAGSLFHIPVARNNNI
EECCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCH
ADVLGQLRSKGLQILATSADGEVDLDDADELLAKPTAWLFGNEAHGLDESLLAQADHRVR
HHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEECCCCCCCCHHHHHCCCCEEE
IPIRGRAESLNLATAASICLYESSKALFAGE
EEECCCCCCCCHHHHHEEEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]