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The map label for this gene is efpA [H]

Identifier: 145295491

GI number: 145295491

Start: 1560268

End: 1561692

Strand: Reverse

Name: efpA [H]

Synonym: cgR_1420

Alternate gene names: 145295491

Gene position: 1561692-1560268 (Counterclockwise)

Preceding gene: 145295494

Following gene: 145295489

Centisome position: 47.12

GC content: 54.95

Gene sequence:

>1425_bases
TTGCAGCGATCAACAATTGGTCACATGATTTTAAGCATCGTCCTTCTGGGCTACTTCATGATTCTGCTTGACACTTCCAT
CGTAATTACTGGACTACCTGCAATCGGCAGTGAACTAGACATCGATCCCGTCCACCTGTCGTGGGTGCAAAGCTCCTATA
CCTTGGTCTTCGGCGCACTTCTCCTACTGGGAGCTCGCGCCGGTGACATTTTCGGCCGAAAGAAAATGCTCTACATTGGT
CTCGCATTGTTTGCGGCCTCATCGTTAGCCATTGCGATCGCTCCAAACGCTGCAGTGCTCATTGCAGCGCGTGTGGTTCA
AGGTGCTGGCGCTGCGATTATCGCCCCAGCGACACTTGCGCTGATTACTGAATTCTTCCCCGAAGGCCCAGCTCGCCTTC
GCGCTACCTCGGCTTATGGTGCTGTTGCTGGCATCGGTGTGGCAGCAGGTCTTGTGATCGGCGGCGTATTTGCTGATCTT
TTGTCGTGGCGTATCGGCTTCTTTATCAACGTCCCCATTGCCACAGCGTTGGCTTACATGGTGCACAAAGCTATTCCCAC
AACCTCCAGCAGGCCTGGATCTCTCGATATCTTCGGCGCAATTACCTCCACCGTTGGTATCGCCGCCGTGCTCTACGCAA
TTGTCCGCAGCGCCGATTACAGCTGGACAGATCCGATTGTGCTGATCTCCCTCGTGCTGGGCATCGCAGTATTCATCTGG
TTCCTCCGCCACGAATCCACAGCCAAAGAACCGCTTCTGCCCCTGGGGCTCTTTAAAAACCACAGGCGAAACACCATCTT
GGCCAGCCGCTTTCTTCTGGTTGGCTCCGTGATGTCATTCTTCTTCTTTGCCACTCAGCTGTTCCAGGACACCATGGGAA
TGAATGCTCTCCGGGCAGGCCTTGCGTTCATTCCGCTATCTCTACTGCAGTTTGCCAGCGCCGCGATGGTGCCACGGCTT
TCCCGAGCAGGCGTATCTGATTCCATTCTCACCGTCATCGGTTTCGCCATCATGGTCATCGGCATGGCAGGCCTCGCATT
TGTACCAAACACGATGATCGCGCTGATCCTACCAATAGTTTTGGTGGGATTTGGCCAAGGCTTCGCTTTCGGACCAATGA
CAGCTCTGGCAGTTCAAGGTGCACCGAAGAACCAATCCGGCGCCGTTTCTGGCCTGGTGAATTCCCTTCACCAAATCGGC
GGCACCTTCGGTTTGGGTGTGTTCTCCTCCTTGGCTGTCGCTGTCATCGGACATGATGCAACATCAGAGATGATCAGCGA
CCGCGCACACTTCGGATTCTTGCTCTCCACCGTGACGCTGACGCTGGCCACCATCTTTGCGGTCACACTGCTGAAGCGCC
ACGAAACTCGAAAGAGTAGCGAGCGCCCACCCCGGCGAAACGAGGAAAAGGCAGTTACCTCTTAG

Upstream 100 bases:

>100_bases
GAGTATCTGCGTGTACATCCATATCTTGAACATAGGCGTTGCGTCTGACTTTTAAACAGGTACCAGCAGTACCGGCATAA
ACGTTCACTGTTGCGGCTTC

Downstream 100 bases:

>100_bases
TGCGCTGCAGCATCCCAGTTAACGCCATCACCAGCTGAAACTTCCAAAGGAACGGACAGCTTGATGGCGTTGTCCATTTC
GCGTTCCAGAATCTCACGGA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MQRSTIGHMILSIVLLGYFMILLDTSIVITGLPAIGSELDIDPVHLSWVQSSYTLVFGALLLLGARAGDIFGRKKMLYIG
LALFAASSLAIAIAPNAAVLIAARVVQGAGAAIIAPATLALITEFFPEGPARLRATSAYGAVAGIGVAAGLVIGGVFADL
LSWRIGFFINVPIATALAYMVHKAIPTTSSRPGSLDIFGAITSTVGIAAVLYAIVRSADYSWTDPIVLISLVLGIAVFIW
FLRHESTAKEPLLPLGLFKNHRRNTILASRFLLVGSVMSFFFFATQLFQDTMGMNALRAGLAFIPLSLLQFASAAMVPRL
SRAGVSDSILTVIGFAIMVIGMAGLAFVPNTMIALILPIVLVGFGQGFAFGPMTALAVQGAPKNQSGAVSGLVNSLHQIG
GTFGLGVFSSLAVAVIGHDATSEMISDRAHFGFLLSTVTLTLATIFAVTLLKRHETRKSSERPPRRNEEKAVTS

Sequences:

>Translated_474_residues
MQRSTIGHMILSIVLLGYFMILLDTSIVITGLPAIGSELDIDPVHLSWVQSSYTLVFGALLLLGARAGDIFGRKKMLYIG
LALFAASSLAIAIAPNAAVLIAARVVQGAGAAIIAPATLALITEFFPEGPARLRATSAYGAVAGIGVAAGLVIGGVFADL
LSWRIGFFINVPIATALAYMVHKAIPTTSSRPGSLDIFGAITSTVGIAAVLYAIVRSADYSWTDPIVLISLVLGIAVFIW
FLRHESTAKEPLLPLGLFKNHRRNTILASRFLLVGSVMSFFFFATQLFQDTMGMNALRAGLAFIPLSLLQFASAAMVPRL
SRAGVSDSILTVIGFAIMVIGMAGLAFVPNTMIALILPIVLVGFGQGFAFGPMTALAVQGAPKNQSGAVSGLVNSLHQIG
GTFGLGVFSSLAVAVIGHDATSEMISDRAHFGFLLSTVTLTLATIFAVTLLKRHETRKSSERPPRRNEEKAVTS
>Mature_474_residues
MQRSTIGHMILSIVLLGYFMILLDTSIVITGLPAIGSELDIDPVHLSWVQSSYTLVFGALLLLGARAGDIFGRKKMLYIG
LALFAASSLAIAIAPNAAVLIAARVVQGAGAAIIAPATLALITEFFPEGPARLRATSAYGAVAGIGVAAGLVIGGVFADL
LSWRIGFFINVPIATALAYMVHKAIPTTSSRPGSLDIFGAITSTVGIAAVLYAIVRSADYSWTDPIVLISLVLGIAVFIW
FLRHESTAKEPLLPLGLFKNHRRNTILASRFLLVGSVMSFFFFATQLFQDTMGMNALRAGLAFIPLSLLQFASAAMVPRL
SRAGVSDSILTVIGFAIMVIGMAGLAFVPNTMIALILPIVLVGFGQGFAFGPMTALAVQGAPKNQSGAVSGLVNSLHQIG
GTFGLGVFSSLAVAVIGHDATSEMISDRAHFGFLLSTVTLTLATIFAVTLLKRHETRKSSERPPRRNEEKAVTS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=408, Percent_Identity=25.9803921568627, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=27.027027027027, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790195, Length=476, Percent_Identity=25, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789042, Length=413, Percent_Identity=24.2130750605327, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788392, Length=423, Percent_Identity=26.241134751773, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=436, Percent_Identity=23.6238532110092, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323520, Length=437, Percent_Identity=24.4851258581236, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=170, Percent_Identity=34.1176470588235, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323935, Length=441, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=22.7979274611399, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49888; Mature: 49888

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSTIGHMILSIVLLGYFMILLDTSIVITGLPAIGSELDIDPVHLSWVQSSYTLVFGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLLGARAGDIFGRKKMLYIGLALFAASSLAIAIAPNAAVLIAARVVQGAGAAIIAPATLA
HHHHCCCCHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
LITEFFPEGPARLRATSAYGAVAGIGVAAGLVIGGVFADLLSWRIGFFINVPIATALAYM
HHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
VHKAIPTTSSRPGSLDIFGAITSTVGIAAVLYAIVRSADYSWTDPIVLISLVLGIAVFIW
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FLRHESTAKEPLLPLGLFKNHRRNTILASRFLLVGSVMSFFFFATQLFQDTMGMNALRAG
HHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LAFIPLSLLQFASAAMVPRLSRAGVSDSILTVIGFAIMVIGMAGLAFVPNTMIALILPIV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVGFGQGFAFGPMTALAVQGAPKNQSGAVSGLVNSLHQIGGTFGLGVFSSLAVAVIGHDA
HHHCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCH
TSEMISDRAHFGFLLSTVTLTLATIFAVTLLKRHETRKSSERPPRRNEEKAVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRSTIGHMILSIVLLGYFMILLDTSIVITGLPAIGSELDIDPVHLSWVQSSYTLVFGAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLLGARAGDIFGRKKMLYIGLALFAASSLAIAIAPNAAVLIAARVVQGAGAAIIAPATLA
HHHHCCCCHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
LITEFFPEGPARLRATSAYGAVAGIGVAAGLVIGGVFADLLSWRIGFFINVPIATALAYM
HHHHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
VHKAIPTTSSRPGSLDIFGAITSTVGIAAVLYAIVRSADYSWTDPIVLISLVLGIAVFIW
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FLRHESTAKEPLLPLGLFKNHRRNTILASRFLLVGSVMSFFFFATQLFQDTMGMNALRAG
HHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LAFIPLSLLQFASAAMVPRLSRAGVSDSILTVIGFAIMVIGMAGLAFVPNTMIALILPIV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVGFGQGFAFGPMTALAVQGAPKNQSGAVSGLVNSLHQIGGTFGLGVFSSLAVAVIGHDA
HHHCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCH
TSEMISDRAHFGFLLSTVTLTLATIFAVTLLKRHETRKSSERPPRRNEEKAVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]