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The map label for this gene is glnH [H]

Identifier: 145295473

GI number: 145295473

Start: 1540247

End: 1541251

Strand: Reverse

Name: glnH [H]

Synonym: cgR_1406

Alternate gene names: 145295473

Gene position: 1541251-1540247 (Counterclockwise)

Preceding gene: 145295475

Following gene: 145295472

Centisome position: 46.5

GC content: 56.02

Gene sequence:

>1005_bases
ATGGCAGGTAGTATTTTGCCCATGATTGAGAGTTTCACCCGATTGCGAATTTCGCAGACACCGCCTCTGAAACGGCTACT
GCATAGCACCTTGCGTTTCCCCAAAATCCCCAAGCGCGCTGTAGCGGCGACCGTCGGCATCGTGGCAACCTCATTCACCT
TGGCTTCTTGTGTCACCAATGAGGAGCAGGGCAACCCAGATGGCTGGGAGCAGATCGTTCCAGATCCTGTACCGGAGATT
CAGGCGATGGTTCCCGAAGCTCTGGCTCAGCGCGGTGTGCTCACCGCCGGTGCCAACCCACCTTTCCCACCGTTTGAGTT
TAAAGATTCCGATGGTCAGATTATCGGTGTGGAAATGGACCTCGTGCGTGCAATGGCGGGGGTGATGGGCTTGGAGTTCA
GCCCTCAGGAGCAGGATTTCTCCCTCATCCTTCCATCAGTTCAAGCTGGCACCCTTGATATCGGAGCCTCTGGCTTCACT
GACAACGAGGAACGCCGCGAGAACTTTGATTTCATCGATTTCCTCTACGCAGGTGTGCAGTGGGCGCAGGCAACTGATCG
TGAAACCCCAATCGATCCGGAAAACGCCTGTGGTCTCACCGTTGCTGTACAGCGCACAACCGTGGCAGAGACCGACGATG
TCCGTCCTCGCTCAGCTCAATGTGAAGCCGAAGGCAAAGAGCCGATCACCATTTTGTCTTATGAAACCGCAGATACTGCA
GCTACCGCATTGATCCTGGGACGCGCAGACGCACTTGCTGCGGACTCCCCTGTTTCAGCTTGGGCTGCAGAGCGCTCCGA
AGGCCGCATCGAAGTTGTGGGCGATATGTACTTGGCTGCGCCATTTGGTTTCGCATTCCCGTTGGAATCTGACCTCACCC
CAGCAGCAGCTGCGGCGTTCCAGCACTTGATTGACACCGGCGATTACCAGCGCATCATGGCGCAATGGGGCATTGAAGAA
GGCCTTCTTGATGAGGCCCTGATCAACGAACAGCCACTCAAGTAG

Upstream 100 bases:

>100_bases
TAGCTGTTTATTTTAAAGTCCCTCCCGAGCCCATGCCGGATAAAACAAGACCCGAGCGCCTGCCAACGCAAAATCCAGCC
TCACTTAAGGTTATTGCACC

Downstream 100 bases:

>100_bases
AGCCTTCCAGCAACTAAAAACAATTAAAGGATTACTTCACAGATGTCAGATCTTAATCAAGGGCCTGGAGCTTCAACGGC
CCAACCAACACCGATTGAAG

Product: hypothetical protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAGSILPMIESFTRLRISQTPPLKRLLHSTLRFPKIPKRAVAATVGIVATSFTLASCVTNEEQGNPDGWEQIVPDPVPEI
QAMVPEALAQRGVLTAGANPPFPPFEFKDSDGQIIGVEMDLVRAMAGVMGLEFSPQEQDFSLILPSVQAGTLDIGASGFT
DNEERRENFDFIDFLYAGVQWAQATDRETPIDPENACGLTVAVQRTTVAETDDVRPRSAQCEAEGKEPITILSYETADTA
ATALILGRADALAADSPVSAWAAERSEGRIEVVGDMYLAAPFGFAFPLESDLTPAAAAAFQHLIDTGDYQRIMAQWGIEE
GLLDEALINEQPLK

Sequences:

>Translated_334_residues
MAGSILPMIESFTRLRISQTPPLKRLLHSTLRFPKIPKRAVAATVGIVATSFTLASCVTNEEQGNPDGWEQIVPDPVPEI
QAMVPEALAQRGVLTAGANPPFPPFEFKDSDGQIIGVEMDLVRAMAGVMGLEFSPQEQDFSLILPSVQAGTLDIGASGFT
DNEERRENFDFIDFLYAGVQWAQATDRETPIDPENACGLTVAVQRTTVAETDDVRPRSAQCEAEGKEPITILSYETADTA
ATALILGRADALAADSPVSAWAAERSEGRIEVVGDMYLAAPFGFAFPLESDLTPAAAAAFQHLIDTGDYQRIMAQWGIEE
GLLDEALINEQPLK
>Mature_333_residues
AGSILPMIESFTRLRISQTPPLKRLLHSTLRFPKIPKRAVAATVGIVATSFTLASCVTNEEQGNPDGWEQIVPDPVPEIQ
AMVPEALAQRGVLTAGANPPFPPFEFKDSDGQIIGVEMDLVRAMAGVMGLEFSPQEQDFSLILPSVQAGTLDIGASGFTD
NEERRENFDFIDFLYAGVQWAQATDRETPIDPENACGLTVAVQRTTVAETDDVRPRSAQCEAEGKEPITILSYETADTAA
TALILGRADALAADSPVSAWAAERSEGRIEVVGDMYLAAPFGFAFPLESDLTPAAAAAFQHLIDTGDYQRIMAQWGIEEG
LLDEALINEQPLK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=28.7610619469027, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=22.4137931034483, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 36052; Mature: 35921

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSILPMIESFTRLRISQTPPLKRLLHSTLRFPKIPKRAVAATVGIVATSFTLASCVTN
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEQGNPDGWEQIVPDPVPEIQAMVPEALAQRGVLTAGANPPFPPFEFKDSDGQIIGVEMD
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCEECCCCCEEEEHHH
LVRAMAGVMGLEFSPQEQDFSLILPSVQAGTLDIGASGFTDNEERRENFDFIDFLYAGVQ
HHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHH
WAQATDRETPIDPENACGLTVAVQRTTVAETDDVRPRSAQCEAEGKEPITILSYETADTA
HHHCCCCCCCCCCCCCCCEEEEEEECEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHH
ATALILGRADALAADSPVSAWAAERSEGRIEVVGDMYLAAPFGFAFPLESDLTPAAAAAF
HHHHHEECCHHHCCCCCHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHH
QHLIDTGDYQRIMAQWGIEEGLLDEALINEQPLK
HHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AGSILPMIESFTRLRISQTPPLKRLLHSTLRFPKIPKRAVAATVGIVATSFTLASCVTN
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEQGNPDGWEQIVPDPVPEIQAMVPEALAQRGVLTAGANPPFPPFEFKDSDGQIIGVEMD
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCEECCCCCEEEEHHH
LVRAMAGVMGLEFSPQEQDFSLILPSVQAGTLDIGASGFTDNEERRENFDFIDFLYAGVQ
HHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHH
WAQATDRETPIDPENACGLTVAVQRTTVAETDDVRPRSAQCEAEGKEPITILSYETADTA
HHHCCCCCCCCCCCCCCCEEEEEEECEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHH
ATALILGRADALAADSPVSAWAAERSEGRIEVVGDMYLAAPFGFAFPLESDLTPAAAAAF
HHHHHEECCHHHCCCCCHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCCCHHHHHHH
QHLIDTGDYQRIMAQWGIEEGLLDEALINEQPLK
HHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]