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The map label for this gene is ddl

Identifier: 145295462

GI number: 145295462

Start: 1528843

End: 1529925

Strand: Direct

Name: ddl

Synonym: cgR_1395

Alternate gene names: 145295462

Gene position: 1528843-1529925 (Clockwise)

Preceding gene: 145295461

Following gene: 145295464

Centisome position: 46.13

GC content: 54.29

Gene sequence:

>1083_bases
GTGAGCAACTCTAATTCCGGAAAAGTCCGCGTCGCAGTCGTTTATGGTGGTCGCAGTTCTGAGCACTCCGTCTCCTGCGT
CTCCGCTGGTGCCATCATGGCCCATCTTGATCCTGAGAAGTACGATGTGATCCCCGTCGGCATTACTGTCGACGGTGCGT
GGGTTGTTGGTGAGACCGATCCGCAGAAGCTAACTCTCATCGATCGCACTATGCCTGAGGTGGAGCATCGTGAAGAGGTT
CGCCCAAGCCTGGATCCTGCACACCGTGGAGAGTTCCACTTTTCCGATGGCAGCTTGTATGCCACCGCTGATGTGATTTT
CCCTGTGCTGCATGGTCGTTTTGGTGAAGACGGCACTGTGCAGGGTCTGTTTGCACTGTCTGATATTCCGGTCGTTGGCC
CAGGTGTGCTGGCCTCTGCTGCGGGAATGGACAAGGAATACACTAAGAAGCTCATGGCAGCGGAAGGCCTGCCCATTGGC
CGTGAGGTGATTCTGCGTGATCGTACCGAGCTGACCGAGGCAGAAAAGAACCTGCTGGGCCTGCCTGTATTTGTGAAGCC
TGCGCGTGGTGGCTCATCGATTGGTATCTCTCGTGTTACTGCGTGGGAGGATTTTAATAAGGCTGTGGGGCTTGCTCGTG
CCCATGATGAGAAGGTCATTGTGGAATCAGAGATCGTTGGCTCTGAGGTGGAGTGTGGCGTGCTGCAGTATCCAGACGGT
CGTATCGTGGCGTCTGTTCCTGCGTTGCTGTCTGGCACCGAATCAGGCGCTGGTGGATTCTATGACTTTGATACCAAGTA
CTTGGACAACGTTGTTACTGCGGAGATCCCAGCACCGCTTGATGAGAAGACCACGGAACTGATCCAGTCTTTGGCTGTGG
AATCTTTCCAGGCTCTTGCGTGCGAAGGCCTTGCCCGCGTGGACTTCTTTGTTACCGCCAATGGTCCTGTGCTCAATGAG
ATCAACACCATGCCAGGATTTACTCCCATTTCCATGTACCCACAGATGTTCACTGCATCAGGCGTCGCCTATGAGGAATT
GTTGGATGTGTTGGTGCAGCAGGCATTGCACCGCGATAATTAA

Upstream 100 bases:

>100_bases
CGGTGTCTGCCACCGGGATATGAAAGTAACTGACATGATTGTGGCTCTCATGGGCAGGTCTAAGAAGGCTGAGTAGTCTT
AGGTTGTAAGCTTCAATGCT

Downstream 100 bases:

>100_bases
CCTCAAATAAAAACAGCCCCACCTCTCAAACAAGGTGGGGCGATTTTTTATGTCTTATTCCGCAGGAACGGCATCTTCAA
TGAAACCAGAGAGGGTGATC

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSNSNSGKVRVAVVYGGRSSEHSVSCVSAGAIMAHLDPEKYDVIPVGITVDGAWVVGETDPQKLTLIDRTMPEVEHREEV
RPSLDPAHRGEFHFSDGSLYATADVIFPVLHGRFGEDGTVQGLFALSDIPVVGPGVLASAAGMDKEYTKKLMAAEGLPIG
REVILRDRTELTEAEKNLLGLPVFVKPARGGSSIGISRVTAWEDFNKAVGLARAHDEKVIVESEIVGSEVECGVLQYPDG
RIVASVPALLSGTESGAGGFYDFDTKYLDNVVTAEIPAPLDEKTTELIQSLAVESFQALACEGLARVDFFVTANGPVLNE
INTMPGFTPISMYPQMFTASGVAYEELLDVLVQQALHRDN

Sequences:

>Translated_360_residues
MSNSNSGKVRVAVVYGGRSSEHSVSCVSAGAIMAHLDPEKYDVIPVGITVDGAWVVGETDPQKLTLIDRTMPEVEHREEV
RPSLDPAHRGEFHFSDGSLYATADVIFPVLHGRFGEDGTVQGLFALSDIPVVGPGVLASAAGMDKEYTKKLMAAEGLPIG
REVILRDRTELTEAEKNLLGLPVFVKPARGGSSIGISRVTAWEDFNKAVGLARAHDEKVIVESEIVGSEVECGVLQYPDG
RIVASVPALLSGTESGAGGFYDFDTKYLDNVVTAEIPAPLDEKTTELIQSLAVESFQALACEGLARVDFFVTANGPVLNE
INTMPGFTPISMYPQMFTASGVAYEELLDVLVQQALHRDN
>Mature_359_residues
SNSNSGKVRVAVVYGGRSSEHSVSCVSAGAIMAHLDPEKYDVIPVGITVDGAWVVGETDPQKLTLIDRTMPEVEHREEVR
PSLDPAHRGEFHFSDGSLYATADVIFPVLHGRFGEDGTVQGLFALSDIPVVGPGVLASAAGMDKEYTKKLMAAEGLPIGR
EVILRDRTELTEAEKNLLGLPVFVKPARGGSSIGISRVTAWEDFNKAVGLARAHDEKVIVESEIVGSEVECGVLQYPDGR
IVASVPALLSGTESGAGGFYDFDTKYLDNVVTAEIPAPLDEKTTELIQSLAVESFQALACEGLARVDFFVTANGPVLNEI
NTMPGFTPISMYPQMFTASGVAYEELLDVLVQQALHRDN

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=40.3361344537815, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1786280, Length=347, Percent_Identity=32.5648414985591, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CORGB (A4QDT4)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138283.1
- STRING:   A4QDT4
- GeneID:   4992223
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1395
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38593; Mature: 38462

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSNSGKVRVAVVYGGRSSEHSVSCVSAGAIMAHLDPEKYDVIPVGITVDGAWVVGETD
CCCCCCCEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEEEEEEECCEEEECCCC
PQKLTLIDRTMPEVEHREEVRPSLDPAHRGEFHFSDGSLYATADVIFPVLHGRFGEDGTV
CCEEEEEHHCCCCHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCCCE
QGLFALSDIPVVGPGVLASAAGMDKEYTKKLMAAEGLPIGREVILRDRTELTEAEKNLLG
EEEEEECCCCEECCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHEECCHHHHHHHHHHHCC
LPVFVKPARGGSSIGISRVTAWEDFNKAVGLARAHDEKVIVESEIVGSEVECGVLQYPDG
CCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCC
RIVASVPALLSGTESGAGGFYDFDTKYLDNVVTAEIPAPLDEKTTELIQSLAVESFQALA
CEEEECHHHHCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
CEGLARVDFFVTANGPVLNEINTMPGFTPISMYPQMFTASGVAYEELLDVLVQQALHRDN
HCCCEEEEEEEECCCCEEHHCCCCCCCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSNSGKVRVAVVYGGRSSEHSVSCVSAGAIMAHLDPEKYDVIPVGITVDGAWVVGETD
CCCCCCEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEEEEEEECCEEEECCCC
PQKLTLIDRTMPEVEHREEVRPSLDPAHRGEFHFSDGSLYATADVIFPVLHGRFGEDGTV
CCEEEEEHHCCCCHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCCCE
QGLFALSDIPVVGPGVLASAAGMDKEYTKKLMAAEGLPIGREVILRDRTELTEAEKNLLG
EEEEEECCCCEECCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHEECCHHHHHHHHHHHCC
LPVFVKPARGGSSIGISRVTAWEDFNKAVGLARAHDEKVIVESEIVGSEVECGVLQYPDG
CCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCEEECCCC
RIVASVPALLSGTESGAGGFYDFDTKYLDNVVTAEIPAPLDEKTTELIQSLAVESFQALA
CEEEECHHHHCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
CEGLARVDFFVTANGPVLNEINTMPGFTPISMYPQMFTASGVAYEELLDVLVQQALHRDN
HCCCEEEEEEEECCCCEEHHCCCCCCCCCHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA