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The map label for this gene is menF [H]

Identifier: 145295433

GI number: 145295433

Start: 1503860

End: 1504990

Strand: Reverse

Name: menF [H]

Synonym: cgR_1370

Alternate gene names: 145295433

Gene position: 1504990-1503860 (Counterclockwise)

Preceding gene: 145295435

Following gene: 145295428

Centisome position: 45.41

GC content: 58.09

Gene sequence:

>1131_bases
ATGTCTGCCCACCGCAACCCCTCTGCCCCCAACACCGCCCCAGATTTCCTCTTATCCAGAGGCCACGGGTCTGTGCGCAC
CCAAGGATCAAAAGAAGTATTCACCGATCCCTGGGAGGCAGTAGACGCCCTTAAACAAGGGCGCGTAGCCATGGTGGTGG
GTGCTTTGCCCTTCGAACGGGACAAACCAGCAGCGCTGACTGTCCCAGAACACATCATCCGCGAAGACGGCCCCCTAGAA
CCCCACGCCTACTACCGGTTTGGACCAGGCTCAGTACTGCACGCCAGCGTCGCAGGAATTGATCCCTCACCGATTGAGCA
TTTACGACGCGTCAATGCAGCCATCAACACCATCAACAACACCGTCTTGAACAAAGTTGTACTCGCACGTGCCGTGGACA
TCTCCTTTGATCCACCCGTTGATCCCCTGTTGGTAGCCGCCCGCTTGATTGATAACTCCCACAACAAGGACGGCTTCATC
GCTGACCTGACCCCCGCAGGTTCAAACTTTGCAGGCCACATGCTGGTTGGATCCTCCCCAGAGGTCCTGATCAAGCGCCA
AGGATCCACCATCACCGCATACCCACTGGCAGGATCCGCACCACGCCTTGCCGATCCAGTACGCGATGAAGCCCAAGGCC
AAAACCTCCTTGCCAGCGCCAAAAACCTAGAAGAACACTCCTACGTGGTCAACCACCTGCGCACCATCCTGGAACCACTG
TGCTCACAATTTGACGCCCCAACAGTTCCTGAACTGACCAAAACCAACGAAATGTGGCACCTCGCAACACCCATCGTTGG
CACCCTCAAGTACCCACACATCACCGCACTAGAACTAGCCATCCGCACACACCCCACCCCCGCGATCTGTGGCACCCCCA
CCGACGCCGCCGAAGCCCTCATCATCGAAGCGGAATCCCCCCGAAACTTCTACGCCGGAGCAGTCGGCTGGTGTGACTCC
ACCGGAGACGGCGAATACATGGTAGCCATCCGCTGCGCCGAAGTATCCGAAGACGGAACCTGGGCCAGAGCATGGGCAGG
CGGAGGCATCGTCGCCGAATCAGACGCCCAAGAAGAGTTTGATGAAACCACCGCGAAGCTCCAAACCATCATGCGCTCGC
TTGGTTTGTGA

Upstream 100 bases:

>100_bases
CAGAAACGAACACGAACATCAGTCATGCAGGCCATGTTAATAGCCCGCACCCACCGCCCGCGCGTTTACCCCAGTTCTAC
TGGGATTAGAATGGCTAACC

Downstream 100 bases:

>100_bases
GGTTGTGGTCTTAAAACACCGACTTCACGTGAGCTGACTTCTCACGCTCAACTTCTCGTGTGCAGACTTCACTCCAGATT
CCGGGCTCTCACTACTGATG

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSAHRNPSAPNTAPDFLLSRGHGSVRTQGSKEVFTDPWEAVDALKQGRVAMVVGALPFERDKPAALTVPEHIIREDGPLE
PHAYYRFGPGSVLHASVAGIDPSPIEHLRRVNAAINTINNTVLNKVVLARAVDISFDPPVDPLLVAARLIDNSHNKDGFI
ADLTPAGSNFAGHMLVGSSPEVLIKRQGSTITAYPLAGSAPRLADPVRDEAQGQNLLASAKNLEEHSYVVNHLRTILEPL
CSQFDAPTVPELTKTNEMWHLATPIVGTLKYPHITALELAIRTHPTPAICGTPTDAAEALIIEAESPRNFYAGAVGWCDS
TGDGEYMVAIRCAEVSEDGTWARAWAGGGIVAESDAQEEFDETTAKLQTIMRSLGL

Sequences:

>Translated_376_residues
MSAHRNPSAPNTAPDFLLSRGHGSVRTQGSKEVFTDPWEAVDALKQGRVAMVVGALPFERDKPAALTVPEHIIREDGPLE
PHAYYRFGPGSVLHASVAGIDPSPIEHLRRVNAAINTINNTVLNKVVLARAVDISFDPPVDPLLVAARLIDNSHNKDGFI
ADLTPAGSNFAGHMLVGSSPEVLIKRQGSTITAYPLAGSAPRLADPVRDEAQGQNLLASAKNLEEHSYVVNHLRTILEPL
CSQFDAPTVPELTKTNEMWHLATPIVGTLKYPHITALELAIRTHPTPAICGTPTDAAEALIIEAESPRNFYAGAVGWCDS
TGDGEYMVAIRCAEVSEDGTWARAWAGGGIVAESDAQEEFDETTAKLQTIMRSLGL
>Mature_375_residues
SAHRNPSAPNTAPDFLLSRGHGSVRTQGSKEVFTDPWEAVDALKQGRVAMVVGALPFERDKPAALTVPEHIIREDGPLEP
HAYYRFGPGSVLHASVAGIDPSPIEHLRRVNAAINTINNTVLNKVVLARAVDISFDPPVDPLLVAARLIDNSHNKDGFIA
DLTPAGSNFAGHMLVGSSPEVLIKRQGSTITAYPLAGSAPRLADPVRDEAQGQNLLASAKNLEEHSYVVNHLRTILEPLC
SQFDAPTVPELTKTNEMWHLATPIVGTLKYPHITALELAIRTHPTPAICGTPTDAAEALIIEAESPRNFYAGAVGWCDST
GDGEYMVAIRCAEVSEDGTWARAWAGGGIVAESDAQEEFDETTAKLQTIMRSLGL

Specific function: Might be involved in the biosynthesis of menaquinone. Converts chorismate to isochorismate [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=337, Percent_Identity=35.9050445103858, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082077, Length=273, Percent_Identity=30.4029304029304, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787518, Length=213, Percent_Identity=32.3943661971831, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788114, Length=204, Percent_Identity=29.9019607843137, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=203, Percent_Identity=33.4975369458128, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 40348; Mature: 40217

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHRNPSAPNTAPDFLLSRGHGSVRTQGSKEVFTDPWEAVDALKQGRVAMVVGALPFER
CCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCC
DKPAALTVPEHIIREDGPLEPHAYYRFGPGSVLHASVAGIDPSPIEHLRRVNAAINTINN
CCCCEEECCHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TVLNKVVLARAVDISFDPPVDPLLVAARLIDNSHNKDGFIADLTPAGSNFAGHMLVGSSP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCC
EVLIKRQGSTITAYPLAGSAPRLADPVRDEAQGQNLLASAKNLEEHSYVVNHLRTILEPL
CEEEEECCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CSQFDAPTVPELTKTNEMWHLATPIVGTLKYPHITALELAIRTHPTPAICGTPTDAAEAL
HHHCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEE
IIEAESPRNFYAGAVGWCDSTGDGEYMVAIRCAEVSEDGTWARAWAGGGIVAESDAQEEF
EEECCCCCCEECCEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEEECCCCHHHH
DETTAKLQTIMRSLGL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAHRNPSAPNTAPDFLLSRGHGSVRTQGSKEVFTDPWEAVDALKQGRVAMVVGALPFER
CCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCC
DKPAALTVPEHIIREDGPLEPHAYYRFGPGSVLHASVAGIDPSPIEHLRRVNAAINTINN
CCCCEEECCHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TVLNKVVLARAVDISFDPPVDPLLVAARLIDNSHNKDGFIADLTPAGSNFAGHMLVGSSP
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCC
EVLIKRQGSTITAYPLAGSAPRLADPVRDEAQGQNLLASAKNLEEHSYVVNHLRTILEPL
CEEEEECCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
CSQFDAPTVPELTKTNEMWHLATPIVGTLKYPHITALELAIRTHPTPAICGTPTDAAEAL
HHHCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEE
IIEAESPRNFYAGAVGWCDSTGDGEYMVAIRCAEVSEDGTWARAWAGGGIVAESDAQEEF
EEECCCCCCEECCEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEEECCCCHHHH
DETTAKLQTIMRSLGL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]