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The map label for this gene is leuB

Identifier: 145295427

GI number: 145295427

Start: 1497252

End: 1498274

Strand: Direct

Name: leuB

Synonym: cgR_1364

Alternate gene names: 145295427

Gene position: 1497252-1498274 (Clockwise)

Preceding gene: 145295426

Following gene: 145295429

Centisome position: 45.18

GC content: 56.79

Gene sequence:

>1023_bases
ATGAAACTTGCTGTTATTGGTGGAGACGGTATCGGCCCAGAGGTTACTGCAGAAGCCCTCAAGGTTCTAAACGCTGTCCG
CGACGACATCGAGACCACCGATTATGACCTTGGCGCACGCCGTTACCTCAAAAATGGCGAGCTGCTCACCGACGAGGATC
TGGCATCCCTGCGCGAGCATGACGCGATCCTTCTTGGCGCTATCGGTGCACCAGGTTCCGTCCCTCCAGGAATTCTCGAG
CGCGGTTTGCTGCTGAAGATGCGATTCGCACTGGATCACCACGTGAACCTGCGCCCATCCAAGCTGTACGACGGCGTGGA
GTCCCCACTGCGTAACCCAGGCAAGATTGATTTCGTTGTGGTCCGCGAAGGTACCGAAGGCGCCTACACTGGCAACGGTG
GAGCAATCCGCGTGGGAACCGCTCACGAGATTGCCAATGAAACCTCCGTGAACACTCGCTACGGCGCTGAGCGCGTTATT
CGCTACGCATTCGAGCTGGCACAGAGCCGCCGCAAGAAACTCACCCTCGTGCACAAGACCAACGTCTTGGTTCACGGTGG
TGGCCTGTGGCAGCGCACCGTAGATGAGGTTGCAAAGGAATACCCAGAGGTAGCCGTCGATTACAACCACATCGATGCAG
CAACCATCTATCTGATCACTGATCCTTCCCGCTTCGATGTGATTGTTACCGATAACCTCTTCGGCGACATCCTCACCGAT
GAGGCAGGCGCAGTCTCTGGCGGAATTGGCCTCGCAGCTTCCGGAAACATTGATGCCACGGGCACCAACCCTTCCATGTT
TGAGCCAGTTCACGGATCTGCGCCTGATATCGCAGGCCAGGGAATCGCAGACCCAACGGCAGCAATCCTATCCGCTGCGA
TGTTGCTGCGTCACTTAGGTGATGAGGACAACGCAGTACGTATTGAAACAGCCATCGCGGCTGATGTGGCTGGCCGAGAT
AACTCTCAGCCGATTTCTACCACTGAGGTGGGAGACCGCATCGTCAAGGCGCTGCAAAGCTAA

Upstream 100 bases:

>100_bases
GCCCTGCATGATGGGGTAGTGGGGGTTGTTGGGCAGGTACGAGCTGTGATCAATCAGCTACACTAGTGAAGTCCATATAG
TGAGAAGGGAATCCCACAAC

Downstream 100 bases:

>100_bases
ATTTCGAGGCCTAACCCCTTATCTTTATGGTGAGGGGTTCACTTTTCGGTATGGGTTGAATTGGTTGGTGTAAATACTTG
CTGGATTGATATCGGGGACG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTAHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLITDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS

Sequences:

>Translated_340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTAHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLITDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS
>Mature_340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTAHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLITDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=315, Percent_Identity=35.2380952380952, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI4758582, Length=324, Percent_Identity=31.1728395061728, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI28178838, Length=316, Percent_Identity=31.6455696202532, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178821, Length=312, Percent_Identity=29.4871794871795, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=29.746835443038, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI28178819, Length=163, Percent_Identity=35.5828220858896, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=43.2584269662921, Blast_Score=270, Evalue=7e-74,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=41.8539325842697, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1787381, Length=395, Percent_Identity=28.6075949367089, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71986051, Length=312, Percent_Identity=34.9358974358974, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=302, Percent_Identity=32.7814569536424, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=30.5084745762712, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=29.2537313432836, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=319, Percent_Identity=35.7366771159875, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=34.6153846153846, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=32.378223495702, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=32.033426183844, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24643270, Length=311, Percent_Identity=35.6913183279743, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24643268, Length=311, Percent_Identity=35.6913183279743, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=33.0409356725146, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281362242, Length=300, Percent_Identity=29.6666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648872, Length=300, Percent_Identity=29.6666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078633, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24650122, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI161078635, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI161078637, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=30.1449275362319, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20130355, Length=313, Percent_Identity=27.4760383386581, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_CORGB (A4QDP9)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138248.1
- ProteinModelPortal:   A4QDP9
- SMR:   A4QDP9
- STRING:   A4QDP9
- GeneID:   4994502
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1364
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   AATIFMV
- ProtClustDB:   PRK03437
- GO:   GO:0005737
- HAMAP:   MF_01035
- InterPro:   IPR019818
- InterPro:   IPR001804
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 36133; Mature: 36133

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 88-88 BINDING 98-98 BINDING 122-122 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEECHHHHHHHHHC
DAILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
VREGTEGAYTGNGGAIRVGTAHEIANETSVNTRYGAERVIRYAFELAQSRRKKLTLVHKT
EEECCCCCEECCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
NVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLITDPSRFDVIVTDNLFGDILTD
EEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHCC
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEDNAVRIETAIAADVAGRDNSQPISTTEVGDRIVKALQS
CCCCEEEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEECHHHHHHHHHC
DAILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
VREGTEGAYTGNGGAIRVGTAHEIANETSVNTRYGAERVIRYAFELAQSRRKKLTLVHKT
EEECCCCCEECCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
NVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLITDPSRFDVIVTDNLFGDILTD
EEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHCC
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEDNAVRIETAIAADVAGRDNSQPISTTEVGDRIVKALQS
CCCCEEEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA