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The map label for this gene is ilvD1 [H]

Identifier: 145295407

GI number: 145295407

Start: 1476225

End: 1478066

Strand: Reverse

Name: ilvD1 [H]

Synonym: cgR_1344

Alternate gene names: 145295407

Gene position: 1478066-1476225 (Counterclockwise)

Preceding gene: 145295408

Following gene: 145295406

Centisome position: 44.6

GC content: 58.9

Gene sequence:

>1842_bases
ATGATCCCACTTCGTTCAAAAGTCACCACCGTCGGTCGCAATGCAGCTGGCGCTCGCGCCCTTTGGCGTGCCACCGGCAC
CAAGGAAAATGAGTTCGGCAAGCCAATTGTTGCCATCGTGAACTCCTACACCCAGTTCGTGCCCGGACACGTTCACCTTA
AGAACGTCGGCGATATTGTGGCAGATGCAGTGCGCAAAGCCGGTGGCGTTCCAAAAGAATTCAACACCATCGCCGTCGAT
GACGGCATCGCCATGGGACACGGCGGCATGCTGTACTCCCTGCCATCCCGTGAAATCATCGCCGACTCCGTCGAATACAT
GGTCAACGCACACACCGCCGACGCCATGGTGTGTATCTCCAACTGTGACAAGATCACCCCAGGCATGCTCAACGCAGCAA
TGCGCCTGAACATCCCAGTGGTCTTCGTTTCCGGTGGCCCAATGGAAGCTGGCAAGGCTGTCGTCGTTGACGGCGTTGCA
CACGCACCAACCGACCTCATCACCGCGATCTCCGCATCCGCAAGCGATGCAGTCGACGACGCAGGCCTTGCAGCCGTTGA
AGCATCCGCATGCCCAACCTGTGGCTCCTGCTCCGGTATGTTCACCGCGAACTCCATGAACTGCCTCACCGAAGCTCTGG
GACTTTCTCTCCCAGGCAACGGCTCCACCCTGGCAACCCACGCAGCACGTCGCGCACTGTTTGAAAAGGCCGGCGAAACC
GTCGTTGAACTGTGCCGCCGCTACTACGGTGAAGAAGACGAATCCGTTCTGCCACGTGGCATTGCCACCAAGAAGGCATT
CGAAAACGCAATGGCACTGGATATGGCCATGGGTGGATCCACCAACACCATCCTCCACATCCTCGCAGCTGCCCAGGAAG
GCGAAGTTGACTTCGACCTCGCAGACATCGACGAACTGTCCAAAAACGTCCCCTGCCTGTCCAAGGTTGCACCAAACTCC
GACTACCACATGGAAGACGTCCACCGCGCCGGTGGCATTCCAGCACTGCTCGGCGAGCTCAACCGCGGTGGCCTGCTGAA
TAAGGACGTCCACTCCGTTCACTCCAACGACCTTGAAGGTTGGTTGGATGACTGGGATATCCGCTCTGGCAAGACCACCG
AAGTAGCAACCGAACTCTTCCACGCAGCCCCAGGTGGCATCCGCACCACCGAAGCATTCTCCACCGAGAACCGCTGGGAC
GAACTCGACACCGACGCTGCCAAGGGCTGCATCCGCGACGTTGAACACGCCTACACCGCCGACGGCGGCCTGGTTGTTCT
TCGCGGCAACATCTCCCCTGACGGCGCAGTGATCAAGTCCGCAGGTATCGAAGAAGAGCTGTGGAACTTCACCGGACCAG
CACGAGTTGTCGAAAGCCAGGAAGAGGCAGTCTCTGTCATCCTGACCAAGACCATCCAAGCTGGCGAAGTTCTGGTCGTC
CGCTACGAAGGCCCATCAGGTGGACCAGGCATGCAGGAAATGCTTCACCCAACCGCATTCCTCAAGGGATCCGGCCTGGG
CAAGAAGTGTGCACTGATCACCGACGGCCGTTTCTCCGGAGGTTCCTCAGGACTGTCCATCGGCCACGTCTCCCCAGAAG
CAGCACACGGCGGAGTCATTGGTCTGATCGAAAACGGCGACATCGTTTCCATCGACGTTCACAACCGCAAGCTCGAAGTT
CAGGTCTCCAACGAGGAACTCCAGCGCCGCCGCGACGCTATGAACGCCTCCGAGAAGCCATGGCAGCCAGTCAACCGTAA
CCGCGTTGTCACCAAGGCACTGCGCGCATACGCAAAGATGGCTACCTCCGCTGATAAGGGTGCAGTCCGTCAGGTCGACT
AA

Upstream 100 bases:

>100_bases
CCACACCTCTGCACGAGTAGAAGCTCACCCAAGTTTTCAAAGTGCCGTTGATTCTTGACAACCACCCGCCGCTCCTTAGA
GCAGATTTGAAAAGCGCATC

Downstream 100 bases:

>100_bases
CCCTTTGTAAGTGTTTGAGCACCGGTTCCCTACTTTGGGTTCCGGTGCTTTTTCATGTCTTGGGCTGTGTGGGCGTGGTG
GAGCTCCCCGTTGCAAATAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 1 [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MIPLRSKVTTVGRNAAGARALWRATGTKENEFGKPIVAIVNSYTQFVPGHVHLKNVGDIVADAVRKAGGVPKEFNTIAVD
DGIAMGHGGMLYSLPSREIIADSVEYMVNAHTADAMVCISNCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKAVVVDGVA
HAPTDLITAISASASDAVDDAGLAAVEASACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHAARRALFEKAGET
VVELCRRYYGEEDESVLPRGIATKKAFENAMALDMAMGGSTNTILHILAAAQEGEVDFDLADIDELSKNVPCLSKVAPNS
DYHMEDVHRAGGIPALLGELNRGGLLNKDVHSVHSNDLEGWLDDWDIRSGKTTEVATELFHAAPGGIRTTEAFSTENRWD
ELDTDAAKGCIRDVEHAYTADGGLVVLRGNISPDGAVIKSAGIEEELWNFTGPARVVESQEEAVSVILTKTIQAGEVLVV
RYEGPSGGPGMQEMLHPTAFLKGSGLGKKCALITDGRFSGGSSGLSIGHVSPEAAHGGVIGLIENGDIVSIDVHNRKLEV
QVSNEELQRRRDAMNASEKPWQPVNRNRVVTKALRAYAKMATSADKGAVRQVD

Sequences:

>Translated_613_residues
MIPLRSKVTTVGRNAAGARALWRATGTKENEFGKPIVAIVNSYTQFVPGHVHLKNVGDIVADAVRKAGGVPKEFNTIAVD
DGIAMGHGGMLYSLPSREIIADSVEYMVNAHTADAMVCISNCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKAVVVDGVA
HAPTDLITAISASASDAVDDAGLAAVEASACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHAARRALFEKAGET
VVELCRRYYGEEDESVLPRGIATKKAFENAMALDMAMGGSTNTILHILAAAQEGEVDFDLADIDELSKNVPCLSKVAPNS
DYHMEDVHRAGGIPALLGELNRGGLLNKDVHSVHSNDLEGWLDDWDIRSGKTTEVATELFHAAPGGIRTTEAFSTENRWD
ELDTDAAKGCIRDVEHAYTADGGLVVLRGNISPDGAVIKSAGIEEELWNFTGPARVVESQEEAVSVILTKTIQAGEVLVV
RYEGPSGGPGMQEMLHPTAFLKGSGLGKKCALITDGRFSGGSSGLSIGHVSPEAAHGGVIGLIENGDIVSIDVHNRKLEV
QVSNEELQRRRDAMNASEKPWQPVNRNRVVTKALRAYAKMATSADKGAVRQVD
>Mature_613_residues
MIPLRSKVTTVGRNAAGARALWRATGTKENEFGKPIVAIVNSYTQFVPGHVHLKNVGDIVADAVRKAGGVPKEFNTIAVD
DGIAMGHGGMLYSLPSREIIADSVEYMVNAHTADAMVCISNCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKAVVVDGVA
HAPTDLITAISASASDAVDDAGLAAVEASACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHAARRALFEKAGET
VVELCRRYYGEEDESVLPRGIATKKAFENAMALDMAMGGSTNTILHILAAAQEGEVDFDLADIDELSKNVPCLSKVAPNS
DYHMEDVHRAGGIPALLGELNRGGLLNKDVHSVHSNDLEGWLDDWDIRSGKTTEVATELFHAAPGGIRTTEAFSTENRWD
ELDTDAAKGCIRDVEHAYTADGGLVVLRGNISPDGAVIKSAGIEEELWNFTGPARVVESQEEAVSVILTKTIQAGEVLVV
RYEGPSGGPGMQEMLHPTAFLKGSGLGKKCALITDGRFSGGSSGLSIGHVSPEAAHGGVIGLIENGDIVSIDVHNRKLEV
QVSNEELQRRRDAMNASEKPWQPVNRNRVVTKALRAYAKMATSADKGAVRQVD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=612, Percent_Identity=65.5228758169935, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=550, Percent_Identity=28.9090909090909, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI2367371, Length=558, Percent_Identity=30.1075268817204, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1786464, Length=526, Percent_Identity=30.7984790874525, Blast_Score=170, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322476, Length=609, Percent_Identity=34.3185550082102, Blast_Score=321, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 64674; Mature: 64674

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLRSKVTTVGRNAAGARALWRATGTKENEFGKPIVAIVNSYTQFVPGHVHLKNVGDIV
CCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHH
ADAVRKAGGVPKEFNTIAVDDGIAMGHGGMLYSLPSREIIADSVEYMVNAHTADAMVCIS
HHHHHHHCCCCCCCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEC
NCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKAVVVDGVAHAPTDLITAISASASDAVDD
CCCCCCCHHHHHEEEECCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCC
AGLAAVEASACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHAARRALFEKAGET
CCCEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
VVELCRRYYGEEDESVLPRGIATKKAFENAMALDMAMGGSTNTILHILAAAQEGEVDFDL
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCH
ADIDELSKNVPCLSKVAPNSDYHMEDVHRAGGIPALLGELNRGGLLNKDVHSVHSNDLEG
HHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCCCCCCC
WLDDWDIRSGKTTEVATELFHAAPGGIRTTEAFSTENRWDELDTDAAKGCIRDVEHAYTA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHCCCHHHHHHHHHHHHHHCC
DGGLVVLRGNISPDGAVIKSAGIEEELWNFTGPARVVESQEEAVSVILTKTIQAGEVLVV
CCCEEEEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHCHHHHHHHHHHHCCCCCCEEEE
RYEGPSGGPGMQEMLHPTAFLKGSGLGKKCALITDGRFSGGSSGLSIGHVSPEAAHGGVI
EECCCCCCCCHHHHHCHHHHEECCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCEE
GLIENGDIVSIDVHNRKLEVQVSNEELQRRRDAMNASEKPWQPVNRNRVVTKALRAYAKM
EEEECCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ATSADKGAVRQVD
HCCCCCCCCCCCC
>Mature Secondary Structure
MIPLRSKVTTVGRNAAGARALWRATGTKENEFGKPIVAIVNSYTQFVPGHVHLKNVGDIV
CCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHH
ADAVRKAGGVPKEFNTIAVDDGIAMGHGGMLYSLPSREIIADSVEYMVNAHTADAMVCIS
HHHHHHHCCCCCCCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEC
NCDKITPGMLNAAMRLNIPVVFVSGGPMEAGKAVVVDGVAHAPTDLITAISASASDAVDD
CCCCCCCHHHHHEEEECCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCC
AGLAAVEASACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHAARRALFEKAGET
CCCEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
VVELCRRYYGEEDESVLPRGIATKKAFENAMALDMAMGGSTNTILHILAAAQEGEVDFDL
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCH
ADIDELSKNVPCLSKVAPNSDYHMEDVHRAGGIPALLGELNRGGLLNKDVHSVHSNDLEG
HHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHCCCCCCC
WLDDWDIRSGKTTEVATELFHAAPGGIRTTEAFSTENRWDELDTDAAKGCIRDVEHAYTA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHCCCHHHHHHHHHHHHHHCC
DGGLVVLRGNISPDGAVIKSAGIEEELWNFTGPARVVESQEEAVSVILTKTIQAGEVLVV
CCCEEEEECCCCCCCCEEECCCCHHHHHCCCCCHHHHHCHHHHHHHHHHHCCCCCCEEEE
RYEGPSGGPGMQEMLHPTAFLKGSGLGKKCALITDGRFSGGSSGLSIGHVSPEAAHGGVI
EECCCCCCCCHHHHHCHHHHEECCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCEE
GLIENGDIVSIDVHNRKLEVQVSNEELQRRRDAMNASEKPWQPVNRNRVVTKALRAYAKM
EEEECCCEEEEEECCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ATSADKGAVRQVD
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]