Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 145295383

GI number: 145295383

Start: 1451121

End: 1453163

Strand: Direct

Name: ligA

Synonym: cgR_1321

Alternate gene names: 145295383

Gene position: 1451121-1453163 (Clockwise)

Preceding gene: 145295380

Following gene: 145295385

Centisome position: 43.79

GC content: 55.85

Gene sequence:

>2043_bases
GTGACTGAAGATAATGCTCAACTGCGTAGAACGTGGAACGACTTAGCCGAGAAGGTTCGTTATCACCGAGATCGTTATTA
CAACGAACAGCCAGAGATCCCTGATGCTGATTTTGATGCGCTTTTTAAACAGCTTCAGCAGTTGGAAGAAGACCACCCGG
AGCTCGCCGTCCCTGATAGCCCCACCATGGTTGTGGGCGCTCCGGTGGCAGAGCAATCAAGCTTTGACAATGTTGAGCAC
TTGGAGCGAATGCTCAGCTTGGACAATGTTTTTGATGAGCAGGAGTTGCGTGATTGGTTGGGCAGGACGCCAGCCAAGCA
GTATTTGACGGAGTTGAAAATTGATGGCTTGTCCATCGACTTGGTGTATCGCAATGGCCAGTTAGAGCGTGCCGCTACTC
GTGGTGATGGTCGCGTGGGCGAGGACATCACGGCCAATGCTCGCGTGATCGAAGATATCCCGCACCAGCTTCAGGGCACT
GATGAATATCCTGTGCCTGCTGTGCTGGAGATTCGCGGTGAGGTGTTCATCACTGTGGAGGATTTCCCAGAGGTCAACGC
GCAGCGCATTGCTGATGGTGGCAAGCCGTTTGCCAACCCGCGTAATGCTGCGGCTGGTTCTCTGCGTCAGAAAAATATTG
AGGACGTGAAGAAGCGTCGCCTGCGGATGATCAGCCATGGCATCGGTTTCACTGAAGGCTTTAGCCCTGCGTCTCAGCAT
GATGCGTATCTGGCATTGGCTGCCTGGGGTTTGCCCACCTCGCCGTACACAGAGGCTGTGACTGATCCAGAAGATGTGGT
GAAAAAGGTCAGCTACTGGGCTGATCACCGCCACGACGCACTCCATGAGATGGATGGCCTGGTAATTAAGGTCGATGACA
TCGCATCTCAGCGTGCTTTGGGCTCCACCAGCCGCGCGCCTCGCTGGGCCATTGCGTACAAGTACCCTCCGGAGGAGGTC
ACCACCAAGCTGCTTGATATTCAGGTTGGCGTTGGTCGCACCGGCCGTGTGACCCCATTCGCGGTCATGGAGCCTGTTCT
TGTTGCAGGATCAACGGTGTCTATGGCGACGCTGCATAACCAGAGCGAAGTCAAGCGTAAAGGCGTACTCATCGGTGACA
CCGTGGTTATCCGCAAGGCGGGCGAGGTTATCCCAGAGGTGCTTGGCCCTGTCGTAGAGCTTCGTGACGGCACAGAGCGC
GAGTACATCTTCCCAACGCTGTGCCCAGAATGCGGTACCCGTCTGGCGCCCGCGAAGGCCGATGACGTGGATTGGCGTTG
CCCCAACATGCAAAGCTGTCCAGGTCAGCTGTCCACGCGTTTGACCTACCTTGCTGGTCGTGGCGCTTTTGATATTGAAG
CATTGGGCGAAAAGGGCGCTGAAGACCTCATCCGCACCGGCATTTTGCTTGACGAGTCTGGCCTGTTCGACCTCACAGAG
GACGATCTGCTGAGCTCCAATGTCTACACCACCAACGCCGGCAAAGTGAATGCCAGCGGCAAGAAACTGCTGGACAACCT
GCAAAAATCCAAGCAGACCGACCTCTGGCGAGTCCTCGTGGCACTATCTATCAGGCACGTAGGCCCCACCGCAGCGCGCG
CCCTTGCAGGTCGCTATCACTCCATCCAGGCGCTTATCGACGCCCCCCTCGAGGAACTCTCCGAAACCGATGGAGTAGGT
ACCATCATTGCCCAATCCTTCAAGGACTGGTTCGAGGTTGATTGGCACAAGGCCATCGTGGACAAGTGGGCAGCCGCTGG
TGTGACTATGGAGGAAGAAGTAGGGGAGGTCGCTGAACAAACCCTTGAAGGCCTAACCATCGTGGTCACCGGAGGATTGG
AAGGCTTCACCAGAGATTCGGTGAAGGAAGCCATCATCTCCCGTGGCGGAAAAGCCTCTGGATCTGTCTCGAAAAAAACT
GACTACGTGGTGGTTGGTGAAAACGCAGGTTCCAAGGCCACCAAGGCAGAAGAACTAGGGCTGCGCATTCTGGATGAGGC
AGGATTCGTCCGTTTGCTCAATACCGGCTCAGCTGACGAATAG

Upstream 100 bases:

>100_bases
AGGTCTGCACCCATTCATACGCGAATTTTGACGTGAGTTTTAGCTTTCCGAGCACCTGTGCGAATGGCCGTGTCCACCCT
TAGGGTTAGAATCGGGAATC

Downstream 100 bases:

>100_bases
TCGCACATGAAAATGCCCCAAGGAAGATCTTCCTGGGGGCATTTTTTAAACAGCTCAGATTATTTTTGAAGCATTGCTCC
GATAATGTCACCGACTTGGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEH
LERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGT
DEYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEV
TTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTER
EYIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVG
TIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKT
DYVVVGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE

Sequences:

>Translated_680_residues
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEH
LERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGT
DEYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEV
TTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTER
EYIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVG
TIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKT
DYVVVGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE
>Mature_679_residues
TEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEHL
ERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTD
EYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQHD
AYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEVT
TKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTERE
YIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTED
DLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVGT
IIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTD
YVVVGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=85, Percent_Identity=48.2352941176471, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788750, Length=687, Percent_Identity=39.7379912663755, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87082305, Length=457, Percent_Identity=24.2888402625821, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CORGB (A4QDK5)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138204.1
- ProteinModelPortal:   A4QDK5
- STRING:   A4QDK5
- GeneID:   4993372
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1321
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74693; Mature: 74562

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 113-113 BINDING 111-111 BINDING 134-134 BINDING 174-174 BINDING 290-290 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCC
PTMVVGAPVAEQSSFDNVEHLERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSID
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHEECCEEEE
LVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTDEYPVPAVLEIRGEVFITVE
EEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCEEEEECCCEEEEEC
DFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRAL
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHC
GSTSRAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHN
CCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHCC
QSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTEREYIFPTLCPECGTRLAPAKA
HHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHCHHHCCCCCCCCC
DDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHCCEECCCCCCCCCH
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYH
HHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SIQALIDAPLEELSETDGVGTIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTDYVVVGENAGSKATKAEELG
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHC
LRILDEAGFVRLLNTGSADE
CEEECCCCEEEEECCCCCCC
>Mature Secondary Structure 
TEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCC
PTMVVGAPVAEQSSFDNVEHLERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSID
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHEECCEEEE
LVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTDEYPVPAVLEIRGEVFITVE
EEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCEEEEECCCEEEEEC
DFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRAL
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHC
GSTSRAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHN
CCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHCC
QSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTEREYIFPTLCPECGTRLAPAKA
HHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHCHHHCCCCCCCCC
DDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHCCEECCCCCCCCCH
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYH
HHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SIQALIDAPLEELSETDGVGTIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTDYVVVGENAGSKATKAEELG
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHC
LRILDEAGFVRLLNTGSADE
CEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA