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The map label for this gene is glgE [H]

Identifier: 145295365

GI number: 145295365

Start: 1432290

End: 1434047

Strand: Reverse

Name: glgE [H]

Synonym: cgR_1303

Alternate gene names: 145295365

Gene position: 1434047-1432290 (Counterclockwise)

Preceding gene: 145295372

Following gene: 145295364

Centisome position: 43.27

GC content: 55.12

Gene sequence:

>1758_bases
GTGGATGCGTGGTCGGATCCGATGGCTACATGGCGTCATGCGATTACCACTAAGATTGAGGCTGGTCAGGGTTCGGATGA
GTTGTATAACGACTTCGAGCATGGGGCTCAGCTGTTTGAACGGGCTGCGGAGAATTTGTCTAAAGAGGATAGGACTGCGC
TTTTCGACGTCGCCTCCTCTCTGCGGCGTGGCGGCGATGTCCGCGCACGTCTCGCCCCAGCGCTCACCGCGAGTGTCACT
CGTCTTCTAGAACTTAACCCGTTGCGTGAGTTGGTCACGATGGGTGAAAACCTGCAGGTTCGTGTCGAGCGTCGTGCCGC
TTTGGTCAACTCTTGGTATGAGCTTTTCCCTCGTTCCACAGGTGGTTGGGATGAGTCCGGCACCCCCGTTCATGGCACTT
TCGCTACCACTGCTCAGGCGTTGGAGCGTGTCGCGAAGATGGGCTTCGATACTGTTTACTTCCCGCCGATCCATCCGATT
GGCGAGGTCAACCGCAAGGGCCGCAATAATACGCTGACCCCGGAACCTCATGATGTGGGTTCGCCGTGGGCTATTGGTTC
TAAAGATGGTGGGCATGATGCAACGCATCCGCGGTTGGGCACCATTGAAGATTTCCAGGCGTTGTTGGCTCGCGCACGGG
AACTCAATTTGGAAGTTGCACTCGATCTAGCTCTGCAGGCTGCCCCTGATCATCCATGGGCGCAGGAACACCGCGAGTTT
TTCACGGTGTTGGCTGATGGCACCATTGCGTATGCAGAAAACCCACCGAAGAAGTACCAGGATATTTATCCGATCAACTT
CGATAACGCCCCTGAGAAGATCTACGCAGAAATTTTCCGTGTGGTGAAGTTCTGGGTGGATTTGGGTGTGACCACATTCC
GCGTGGATAACCCGCACACGAAGCCCGCTAATTTCTGGCAGTGGCTTATTTCTGCCATCCATAAATCAAACCCTGAGGTC
ATTTTCCTAGCGGAGGCGTTTACTCGCCCGGCACGTCTGTATGGCTTGGCCAAGATTGGTTTCTCCCAGTCTTACACCTA
CTTCACGTGGAAGGTCACCAAGGAGGAGCTCACCGAGTTCGCTACTGAGATCGCCCGCATGGCGGATATTTCTCGTCCGA
ACCTGTTTGTGAACACTCCCGACATTTTGCATGCGTCTCTGCAGCATGGTGGACGCGCAATGTTCGCTATCCGCGCCGCG
TTGGCCGCCACGATGTCTCCTGTGTGGGGCGTGTACTCCGGATATGAGCTTTTTGAGCATGAGGCAGTGAAGCCTGGTTC
GGAGGAGTACTTGGATTCTGAGAAGTATGAGCTGCGTCCGCGCGATTTCGAGGGTGCTCTGGAACGTGGCGATTCTCTCG
AGGATTACATCGCTCTGCTCAACCAGATCCGTCGCGCGAACCCTGCCTTGCAGCAGCTACGCAACATCCACTTCCACGAA
GCGGACAATGATCAGATCATCGCCTACTCCAAGGTTGATGCTTTGACCGGAAATACCGTGTTGATTGTGGTCAACTTGGA
TCCACGTAGTGCTCGTGAGGCTACTGTTCGCCTTGATCTTGGAGCGCTTGGCTTAGAAGCGGGTGCACAGTTTGAGGTGC
GCGATGCCATCACTGGTTCTCGTTACCTGTGGTCAGAGACAAACTTTGTCCGCCTTGAGCCGTTGCGCGATGTCGCCCAC
ATCTTTGTTCTTCCTGAGCTTCCAGCGTCTCGCCGTGAACGTCTCGCGTGGCGCGAAATCAAAACCTACCGCGCGTAA

Upstream 100 bases:

>100_bases
TGCTGAGCCTATTCAGATACATATGCGTCCCACTCCTGATAATCAGGATCAGTCGAATGCGTTTTTCGTTCCTGATGTCC
CTGGTGATTGGACTTTTCGT

Downstream 100 bases:

>100_bases
TTTCCCATCTCTGTACCTTCTATCAAGGATTATCATCATGACCGTTGACCCCGCGAGCCACATCACCATCCCTGAGGCAG
ATCTGGCCCGCCTGCGCCAC

Product: hypothetical protein

Products: D-glucose; cholesterol

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MDAWSDPMATWRHAITTKIEAGQGSDELYNDFEHGAQLFERAAENLSKEDRTALFDVASSLRRGGDVRARLAPALTASVT
RLLELNPLRELVTMGENLQVRVERRAALVNSWYELFPRSTGGWDESGTPVHGTFATTAQALERVAKMGFDTVYFPPIHPI
GEVNRKGRNNTLTPEPHDVGSPWAIGSKDGGHDATHPRLGTIEDFQALLARARELNLEVALDLALQAAPDHPWAQEHREF
FTVLADGTIAYAENPPKKYQDIYPINFDNAPEKIYAEIFRVVKFWVDLGVTTFRVDNPHTKPANFWQWLISAIHKSNPEV
IFLAEAFTRPARLYGLAKIGFSQSYTYFTWKVTKEELTEFATEIARMADISRPNLFVNTPDILHASLQHGGRAMFAIRAA
LAATMSPVWGVYSGYELFEHEAVKPGSEEYLDSEKYELRPRDFEGALERGDSLEDYIALLNQIRRANPALQQLRNIHFHE
ADNDQIIAYSKVDALTGNTVLIVVNLDPRSAREATVRLDLGALGLEAGAQFEVRDAITGSRYLWSETNFVRLEPLRDVAH
IFVLPELPASRRERLAWREIKTYRA

Sequences:

>Translated_585_residues
MDAWSDPMATWRHAITTKIEAGQGSDELYNDFEHGAQLFERAAENLSKEDRTALFDVASSLRRGGDVRARLAPALTASVT
RLLELNPLRELVTMGENLQVRVERRAALVNSWYELFPRSTGGWDESGTPVHGTFATTAQALERVAKMGFDTVYFPPIHPI
GEVNRKGRNNTLTPEPHDVGSPWAIGSKDGGHDATHPRLGTIEDFQALLARARELNLEVALDLALQAAPDHPWAQEHREF
FTVLADGTIAYAENPPKKYQDIYPINFDNAPEKIYAEIFRVVKFWVDLGVTTFRVDNPHTKPANFWQWLISAIHKSNPEV
IFLAEAFTRPARLYGLAKIGFSQSYTYFTWKVTKEELTEFATEIARMADISRPNLFVNTPDILHASLQHGGRAMFAIRAA
LAATMSPVWGVYSGYELFEHEAVKPGSEEYLDSEKYELRPRDFEGALERGDSLEDYIALLNQIRRANPALQQLRNIHFHE
ADNDQIIAYSKVDALTGNTVLIVVNLDPRSAREATVRLDLGALGLEAGAQFEVRDAITGSRYLWSETNFVRLEPLRDVAH
IFVLPELPASRRERLAWREIKTYRA
>Mature_585_residues
MDAWSDPMATWRHAITTKIEAGQGSDELYNDFEHGAQLFERAAENLSKEDRTALFDVASSLRRGGDVRARLAPALTASVT
RLLELNPLRELVTMGENLQVRVERRAALVNSWYELFPRSTGGWDESGTPVHGTFATTAQALERVAKMGFDTVYFPPIHPI
GEVNRKGRNNTLTPEPHDVGSPWAIGSKDGGHDATHPRLGTIEDFQALLARARELNLEVALDLALQAAPDHPWAQEHREF
FTVLADGTIAYAENPPKKYQDIYPINFDNAPEKIYAEIFRVVKFWVDLGVTTFRVDNPHTKPANFWQWLISAIHKSNPEV
IFLAEAFTRPARLYGLAKIGFSQSYTYFTWKVTKEELTEFATEIARMADISRPNLFVNTPDILHASLQHGGRAMFAIRAA
LAATMSPVWGVYSGYELFEHEAVKPGSEEYLDSEKYELRPRDFEGALERGDSLEDYIALLNQIRRANPALQQLRNIHFHE
ADNDQIIAYSKVDALTGNTVLIVVNLDPRSAREATVRLDLGALGLEAGAQFEVRDAITGSRYLWSETNFVRLEPLRDVAH
IFVLPELPASRRERLAWREIKTYRA

Specific function: Could be involved in glycogen catabolism [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR013780
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF11896 DUF3416 [H]

EC number: 3.2.1.1

Molecular weight: Translated: 65997; Mature: 65997

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAWSDPMATWRHAITTKIEAGQGSDELYNDFEHGAQLFERAAENLSKEDRTALFDVASS
CCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRRGGDVRARLAPALTASVTRLLELNPLRELVTMGENLQVRVERRAALVNSWYELFPRST
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCC
GGWDESGTPVHGTFATTAQALERVAKMGFDTVYFPPIHPIGEVNRKGRNNTLTPEPHDVG
CCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCC
SPWAIGSKDGGHDATHPRLGTIEDFQALLARARELNLEVALDLALQAAPDHPWAQEHREF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCHHHHHHHH
FTVLADGTIAYAENPPKKYQDIYPINFDNAPEKIYAEIFRVVKFWVDLGVTTFRVDNPHT
HHHHCCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KPANFWQWLISAIHKSNPEVIFLAEAFTRPARLYGLAKIGFSQSYTYFTWKVTKEELTEF
CCHHHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHHHHH
ATEIARMADISRPNLFVNTPDILHASLQHGGRAMFAIRAALAATMSPVWGVYSGYELFEH
HHHHHHHHCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EAVKPGSEEYLDSEKYELRPRDFEGALERGDSLEDYIALLNQIRRANPALQQLRNIHFHE
HCCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
ADNDQIIAYSKVDALTGNTVLIVVNLDPRSAREATVRLDLGALGLEAGAQFEVRDAITGS
CCCCCEEEEEECCEECCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEHHHCCCC
RYLWSETNFVRLEPLRDVAHIFVLPELPASRRERLAWREIKTYRA
CEEECCCCEEEECCHHHHHHEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAWSDPMATWRHAITTKIEAGQGSDELYNDFEHGAQLFERAAENLSKEDRTALFDVASS
CCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRRGGDVRARLAPALTASVTRLLELNPLRELVTMGENLQVRVERRAALVNSWYELFPRST
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCC
GGWDESGTPVHGTFATTAQALERVAKMGFDTVYFPPIHPIGEVNRKGRNNTLTPEPHDVG
CCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCC
SPWAIGSKDGGHDATHPRLGTIEDFQALLARARELNLEVALDLALQAAPDHPWAQEHREF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCHHHHHHHH
FTVLADGTIAYAENPPKKYQDIYPINFDNAPEKIYAEIFRVVKFWVDLGVTTFRVDNPHT
HHHHCCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
KPANFWQWLISAIHKSNPEVIFLAEAFTRPARLYGLAKIGFSQSYTYFTWKVTKEELTEF
CCHHHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHHHHH
ATEIARMADISRPNLFVNTPDILHASLQHGGRAMFAIRAALAATMSPVWGVYSGYELFEH
HHHHHHHHCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EAVKPGSEEYLDSEKYELRPRDFEGALERGDSLEDYIALLNQIRRANPALQQLRNIHFHE
HCCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
ADNDQIIAYSKVDALTGNTVLIVVNLDPRSAREATVRLDLGALGLEAGAQFEVRDAITGS
CCCCCEEEEEECCEECCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEHHHCCCC
RYLWSETNFVRLEPLRDVAHIFVLPELPASRRERLAWREIKTYRA
CEEECCCCEEEECCHHHHHHEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; cholesteryl-beta-D-glucoside

Specific reaction: H2O + cholesteryl-beta-D-glucoside = D-glucose + cholesterol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10542168 [H]