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The map label for this gene is glgB [H]

Identifier: 145295364

GI number: 145295364

Start: 1430057

End: 1432252

Strand: Reverse

Name: glgB [H]

Synonym: cgR_1302

Alternate gene names: 145295364

Gene position: 1432252-1430057 (Counterclockwise)

Preceding gene: 145295365

Following gene: 145295357

Centisome position: 43.22

GC content: 56.88

Gene sequence:

>2196_bases
ATGACCGTTGACCCCGCGAGCCACATCACCATCCCTGAGGCAGATCTGGCCCGCCTGCGCCACTGCAACCATCACGATCC
TCACGGGTTTTATGGTTGGCATGAGACCGAAGCTGGTTCGGTTATCCGCACGCGCCAGATCGGCGCAACGCAGGTTAATT
TGCTTATCGACGACACCTCTCACGTCATGACCCCTATCGGCGACGACATTTTCGCAATTGATTTGAGGCACCGCGAGCGC
GCTGACTATCGCTTGGAAGTCACCTGGCCTGATCAAGAACCGCAGGTCAAGGCTGATCCATACTACTTCCTCCCCACCGT
AGGCGAGATGGATATTTACCTCTTCTCTGAGGGACGCCATGAGCGTTTGTGGGAGGTTCTCGGTGCCAACATCAAGACCT
ACCAAACTGCGCTCGGAACAGTTCGTGGCACCGCATTCACCGTATGGGCTCCAAACGCAATTGGCTGCGCAGTGGTCGGT
GGCTTCAACGGTTGGAATGCATCCCAGCATCCGATGCGTTCCATGGGTGGTTCGGGTCTGTGGGAGCTTTTCATCCCAGG
CATAGAGGAAGGCGAAGTGTACAAATTCGCCCTCCAAACCAAGGAAGGCCACCGCCGTGACAAGGCCGACCCAATGGCTC
GTCGCGCAGAACTGGCACCGGCGACCGGATCTATTGTCGCTTCCTCTGAGTACCAGTGGCAGGATTCCGAGTGGCTGCGC
GAGCGTTCCCAAACTGATCTCCCATCCAAGCCAATGAGTGTCTACGAGGTCCACCTCGGTTCTTGGCGCTGGGGTAAGAA
CTATGAGGATTTGGCTACTGAGCTGGTTGATTACGTCGCAGATCTTGGCTACACCCACGTGGAATTCCTCCCTGTCGCAG
AGCACCCCTTCGGCGGTTCCTGGGGTTACCAGGTCACCGGCTACTACGCACCGACCTCTCGTTGGGGTACTCCAGATCAG
TTCCGTGCGCTAGTCGATGCTTTCCACGCCCGCGGTATTGGCGTGATCATGGACTGGGTCCCCGCTCACTTCCCTAAGGA
TGATTGGGCTCTTGCCCGCTTTGATGGCGAAGCCCTCTATGAACACCCTGACTGGAGGCGCGGCGAACAAAAAGATTGGG
GCACCCTGGTCTTTGACTTCGGTCGCAACGAAGTCCGCAACTTCCTCGTCGCTAATGCGTTGTACTGGATTGAAGAATTC
CACATCGATGGTCTGCGCGTCGACGCCGTGGCCTCCATGCTGTACCTCGATTACTCCCGTGAGCACGGCGAATGGGAACC
AAACATCTACGGTGGTCGCGAGAACCTCGAGGCAGTGCAGTTCCTGCAGGAAATGAACGCCACGGTGCTGCGACTGCACC
CTGGTGCGCTCACCATCGCTGAGGAATCAACTTCATGGCCTGGCGTGACCGCACCAACGTGGGACGGCGGCTTGGGATTC
TCCCTCAAGTGGAACATGGGCTGGATGAACGACACCTTAGAGTACTTCTCCAAAAACCCTGTGCACCGCGCATTCCACCA
CAGTGAGCTCACTTTCTCCTTGGTGTACGCATTCTCTGAGCGTTTTGTACTTCCGATCTCTCACGATGAAGTCGTCCACG
GCAAGGGTTCCCTGTGGGACCGTATGCCTGGCGATACGTGGAACAAGGCCGCTGGTCTTCGCACCTTCCTTGCGTACATG
TGGTCACACCCAGGCAAGAAGCTGCTTTTCATGGGTCAGGAGTTTGGTCAGCGTGAAGAGTGGGCTGAAGGCCAGGGACT
GCCATGGGATATTGTCGACGGCTGGCAAGGCGAGTACCACGAAGCCATCCGCACTCTGACCCGCTCCCTCAACGGTGTCT
ACTCAGACTCCCCTGCGCTGCACACTCAGGATTTCACCGGAGAAGGCTTCACATGGAATAAGGGCGACGACGCCACCAAC
AACATTTTGGCGTTCACGCGTTTCGGCAGCGACGGCTCCCAGATGCTGTGTGTATTCAACCTGTCTGGAACCTCCCAGCC
TGAGTACCAGCTCGGTGTTGCAGCGGGTGGCGAATGGAAGCTCGTTCTCAACACTGATGATGCTGAATTCCTCGGTGCAG
AAAACGATATCGCTACTTCCGTTCAGGCAGCAGCGACACCACGCGATAATTTCGCCTACTCACTCTCACTGCATGTCCCA
GCGATGAGTGCTCAGTTCTACTCACTGCAGAAGTAG

Upstream 100 bases:

>100_bases
GAGCTTCCAGCGTCTCGCCGTGAACGTCTCGCGTGGCGCGAAATCAAAACCTACCGCGCGTAATTTCCCATCTCTGTACC
TTCTATCAAGGATTATCATC

Downstream 100 bases:

>100_bases
GACACAGGAAAATGCATCCTTGGGCCAATACCGGCTCAAGGATACAAATTTTCTACCAAAATGGCTAGTTCGGAGGCCAG
CGGGATCGAATATTCGCGCC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MTVDPASHITIPEADLARLRHCNHHDPHGFYGWHETEAGSVIRTRQIGATQVNLLIDDTSHVMTPIGDDIFAIDLRHRER
ADYRLEVTWPDQEPQVKADPYYFLPTVGEMDIYLFSEGRHERLWEVLGANIKTYQTALGTVRGTAFTVWAPNAIGCAVVG
GFNGWNASQHPMRSMGGSGLWELFIPGIEEGEVYKFALQTKEGHRRDKADPMARRAELAPATGSIVASSEYQWQDSEWLR
ERSQTDLPSKPMSVYEVHLGSWRWGKNYEDLATELVDYVADLGYTHVEFLPVAEHPFGGSWGYQVTGYYAPTSRWGTPDQ
FRALVDAFHARGIGVIMDWVPAHFPKDDWALARFDGEALYEHPDWRRGEQKDWGTLVFDFGRNEVRNFLVANALYWIEEF
HIDGLRVDAVASMLYLDYSREHGEWEPNIYGGRENLEAVQFLQEMNATVLRLHPGALTIAEESTSWPGVTAPTWDGGLGF
SLKWNMGWMNDTLEYFSKNPVHRAFHHSELTFSLVYAFSERFVLPISHDEVVHGKGSLWDRMPGDTWNKAAGLRTFLAYM
WSHPGKKLLFMGQEFGQREEWAEGQGLPWDIVDGWQGEYHEAIRTLTRSLNGVYSDSPALHTQDFTGEGFTWNKGDDATN
NILAFTRFGSDGSQMLCVFNLSGTSQPEYQLGVAAGGEWKLVLNTDDAEFLGAENDIATSVQAAATPRDNFAYSLSLHVP
AMSAQFYSLQK

Sequences:

>Translated_731_residues
MTVDPASHITIPEADLARLRHCNHHDPHGFYGWHETEAGSVIRTRQIGATQVNLLIDDTSHVMTPIGDDIFAIDLRHRER
ADYRLEVTWPDQEPQVKADPYYFLPTVGEMDIYLFSEGRHERLWEVLGANIKTYQTALGTVRGTAFTVWAPNAIGCAVVG
GFNGWNASQHPMRSMGGSGLWELFIPGIEEGEVYKFALQTKEGHRRDKADPMARRAELAPATGSIVASSEYQWQDSEWLR
ERSQTDLPSKPMSVYEVHLGSWRWGKNYEDLATELVDYVADLGYTHVEFLPVAEHPFGGSWGYQVTGYYAPTSRWGTPDQ
FRALVDAFHARGIGVIMDWVPAHFPKDDWALARFDGEALYEHPDWRRGEQKDWGTLVFDFGRNEVRNFLVANALYWIEEF
HIDGLRVDAVASMLYLDYSREHGEWEPNIYGGRENLEAVQFLQEMNATVLRLHPGALTIAEESTSWPGVTAPTWDGGLGF
SLKWNMGWMNDTLEYFSKNPVHRAFHHSELTFSLVYAFSERFVLPISHDEVVHGKGSLWDRMPGDTWNKAAGLRTFLAYM
WSHPGKKLLFMGQEFGQREEWAEGQGLPWDIVDGWQGEYHEAIRTLTRSLNGVYSDSPALHTQDFTGEGFTWNKGDDATN
NILAFTRFGSDGSQMLCVFNLSGTSQPEYQLGVAAGGEWKLVLNTDDAEFLGAENDIATSVQAAATPRDNFAYSLSLHVP
AMSAQFYSLQK
>Mature_730_residues
TVDPASHITIPEADLARLRHCNHHDPHGFYGWHETEAGSVIRTRQIGATQVNLLIDDTSHVMTPIGDDIFAIDLRHRERA
DYRLEVTWPDQEPQVKADPYYFLPTVGEMDIYLFSEGRHERLWEVLGANIKTYQTALGTVRGTAFTVWAPNAIGCAVVGG
FNGWNASQHPMRSMGGSGLWELFIPGIEEGEVYKFALQTKEGHRRDKADPMARRAELAPATGSIVASSEYQWQDSEWLRE
RSQTDLPSKPMSVYEVHLGSWRWGKNYEDLATELVDYVADLGYTHVEFLPVAEHPFGGSWGYQVTGYYAPTSRWGTPDQF
RALVDAFHARGIGVIMDWVPAHFPKDDWALARFDGEALYEHPDWRRGEQKDWGTLVFDFGRNEVRNFLVANALYWIEEFH
IDGLRVDAVASMLYLDYSREHGEWEPNIYGGRENLEAVQFLQEMNATVLRLHPGALTIAEESTSWPGVTAPTWDGGLGFS
LKWNMGWMNDTLEYFSKNPVHRAFHHSELTFSLVYAFSERFVLPISHDEVVHGKGSLWDRMPGDTWNKAAGLRTFLAYMW
SHPGKKLLFMGQEFGQREEWAEGQGLPWDIVDGWQGEYHEAIRTLTRSLNGVYSDSPALHTQDFTGEGFTWNKGDDATNN
ILAFTRFGSDGSQMLCVFNLSGTSQPEYQLGVAAGGEWKLVLNTDDAEFLGAENDIATSVQAAATPRDNFAYSLSLHVPA
MSAQFYSLQK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=638, Percent_Identity=28.6833855799373, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI1789839, Length=710, Percent_Identity=45.7746478873239, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI2367229, Length=311, Percent_Identity=25.08038585209, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554896, Length=607, Percent_Identity=26.0296540362438, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32564391, Length=577, Percent_Identity=25.8232235701906, Blast_Score=154, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320826, Length=678, Percent_Identity=24.9262536873156, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28573410, Length=643, Percent_Identity=26.7496111975117, Blast_Score=183, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 82691; Mature: 82560

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDPASHITIPEADLARLRHCNHHDPHGFYGWHETEAGSVIRTRQIGATQVNLLIDDTS
CCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCC
HVMTPIGDDIFAIDLRHRERADYRLEVTWPDQEPQVKADPYYFLPTVGEMDIYLFSEGRH
HHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCCH
ERLWEVLGANIKTYQTALGTVRGTAFTVWAPNAIGCAVVGGFNGWNASQHPMRSMGGSGL
HHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCE
WELFIPGIEEGEVYKFALQTKEGHRRDKADPMARRAELAPATGSIVASSEYQWQDSEWLR
EEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCHHHHH
ERSQTDLPSKPMSVYEVHLGSWRWGKNYEDLATELVDYVADLGYTHVEFLPVAEHPFGGS
HHHCCCCCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
WGYQVTGYYAPTSRWGTPDQFRALVDAFHARGIGVIMDWVPAHFPKDDWALARFDGEALY
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCHHC
EHPDWRRGEQKDWGTLVFDFGRNEVRNFLVANALYWIEEFHIDGLRVDAVASMLYLDYSR
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEECC
EHGEWEPNIYGGRENLEAVQFLQEMNATVLRLHPGALTIAEESTSWPGVTAPTWDGGLGF
CCCCCCCCEECCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCE
SLKWNMGWMNDTLEYFSKNPVHRAFHHSELTFSLVYAFSERFVLPISHDEVVHGKGSLWD
EEEECCCCHHHHHHHHCCCCHHHHHCCCCHHHEEEEEHHCCEEEEECCCCEECCCCCCCC
RMPGDTWNKAAGLRTFLAYMWSHPGKKLLFMGQEFGQREEWAEGQGLPWDIVDGWQGEYH
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHCCCCCCCCCCCCCCCCHHH
EAIRTLTRSLNGVYSDSPALHTQDFTGEGFTWNKGDDATNNILAFTRFGSDGSQMLCVFN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEEE
LSGTSQPEYQLGVAAGGEWKLVLNTDDAEFLGAENDIATSVQAAATPRDNFAYSLSLHVP
CCCCCCCCEEEEEECCCEEEEEEECCCHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECC
AMSAQFYSLQK
CCCCCHHCCCC
>Mature Secondary Structure 
TVDPASHITIPEADLARLRHCNHHDPHGFYGWHETEAGSVIRTRQIGATQVNLLIDDTS
CCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCC
HVMTPIGDDIFAIDLRHRERADYRLEVTWPDQEPQVKADPYYFLPTVGEMDIYLFSEGRH
HHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCCH
ERLWEVLGANIKTYQTALGTVRGTAFTVWAPNAIGCAVVGGFNGWNASQHPMRSMGGSGL
HHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCE
WELFIPGIEEGEVYKFALQTKEGHRRDKADPMARRAELAPATGSIVASSEYQWQDSEWLR
EEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCHHHHH
ERSQTDLPSKPMSVYEVHLGSWRWGKNYEDLATELVDYVADLGYTHVEFLPVAEHPFGGS
HHHCCCCCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
WGYQVTGYYAPTSRWGTPDQFRALVDAFHARGIGVIMDWVPAHFPKDDWALARFDGEALY
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEECCCHHC
EHPDWRRGEQKDWGTLVFDFGRNEVRNFLVANALYWIEEFHIDGLRVDAVASMLYLDYSR
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHEECC
EHGEWEPNIYGGRENLEAVQFLQEMNATVLRLHPGALTIAEESTSWPGVTAPTWDGGLGF
CCCCCCCCEECCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCE
SLKWNMGWMNDTLEYFSKNPVHRAFHHSELTFSLVYAFSERFVLPISHDEVVHGKGSLWD
EEEECCCCHHHHHHHHCCCCHHHHHCCCCHHHEEEEEHHCCEEEEECCCCEECCCCCCCC
RMPGDTWNKAAGLRTFLAYMWSHPGKKLLFMGQEFGQREEWAEGQGLPWDIVDGWQGEYH
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHCCCCCCCCCCCCCCCCHHH
EAIRTLTRSLNGVYSDSPALHTQDFTGEGFTWNKGDDATNNILAFTRFGSDGSQMLCVFN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCEEEEEEE
LSGTSQPEYQLGVAAGGEWKLVLNTDDAEFLGAENDIATSVQAAATPRDNFAYSLSLHVP
CCCCCCCCEEEEEECCCEEEEEEECCCHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEECC
AMSAQFYSLQK
CCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]