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The map label for this gene is narH [H]

Identifier: 145295327

GI number: 145295327

Start: 1389959

End: 1391554

Strand: Reverse

Name: narH [H]

Synonym: cgR_1267

Alternate gene names: 145295327

Gene position: 1391554-1389959 (Counterclockwise)

Preceding gene: 145295328

Following gene: 145295326

Centisome position: 41.99

GC content: 58.15

Gene sequence:

>1596_bases
ATGAAGGTCATGGCTCAGATCGCAATGATCATGAACTTGGATAAGTGCATTGGCTGCCACACGTGTTCTGTCACGTGTAA
GCAGGCCTGGACCAACCGTCAAGGCACGGAATACATTTGGTTCAACAACGTGGAAACCCGTCCGGGCGTGGGTTATCCCC
GCGGTTGGGAAGATCAGGATAAATGGGAAGGCGGTTGGGTCCGCACCAACTCCGGCAAGTTGAAGCCGAAGGCCGGTGGT
CGGTTGAAGAAGCTGGCGACCATCTTCCACAACCCGAAGCTGCCCACCATCGATGATTACTACGAGCCGTGGACCTATGA
GTACGACAAGTTGCTCTCGGCGCCAGCGGGTCAGAAAACACAGCCCACTGCGCGTCCGGTCAGTCAGTTGGATGGCCGTC
CCATCGATAAAATTGAGTGGTCGTCCAACTGGGATGACAACCTGGGCGGATCCACCACCACGCTTGATCAGGATCCCGTC
CTGGAGAAAATGAACCTCACGGTGCAAAAAGAAATCGAAGATTCCTTCATGTTCTATTTGCCCCGCATCTGCGAGCACTG
CCTCAACCCCACCTGTGTGTCCTCCTGCCCATCCGGTGCCATGTACAAGCGCGCCGAAGACGGCATCGTGTTGGTTGACC
AGGATCAATGCCGCGGCTGGCGCATGTGTGTTTCCGGCTGCCCCTACAAAAAGGTCTACTTCAACCACAAGTCGGGCAAG
GCCGAAAAGTGTACGCTGTGCTATCCGCGCCTCGAGGTCGGCCAGCCGACCGTGTGCTCCGAGACGTGCGTGGGTCGCTT
GCGCTACTTGGGCGTTTTGCTTTACGACGCCGACCGTGTCGCTGAAGTCGCCGCCACGCCAGACGAGAAGGATCTTTTTG
AAGCCCAAAAGACCCTCTTCCTAGATCCCCACGACCCACAGGTGATCGCCGACGCCCAACGCAACGGCATCCCGCACTCC
TGGATCGAAGCTGCGCAGAACTCCCCAATTTACGATCTCATCTTCAAATACGAGGTTGCCCTCCCGCTTCACCCTGAATA
CCGCACCTTGCCGATGGTTTGGTACATTCCGCCACTAAGCCCCATCGTTGATGAGGTGACCGCGTCCGGCAACGACGGCG
AAGACCACAAGATCCTGCTCACCGCGCTGTCCACCATGCGCATCCCGCTGGAATACCTGGCTGGATTGTTCACTGCCGGT
GATACCAGGCCGGTGGAAAAATCCCTCCGACGCCTAGCCGCCATGCGATCATATATGCGCGATATCAGTTTGGGCCGCGA
ACCTCAGGAAGAAATCGCAGAGGCTGTCGGAATGACCGGCAAGGTGGTGCAGGAAATGTATCGCATCCTGGCCATTGCCA
AGTATGACGATCGCTATGTCATCCCCACCGCCTCCCCTGAGACCCCGCGCGGAATTTCGTCCCTGGATCCTTTCGGCGAT
GTCGATCCAGCCCGAGCCACCGAGCAGCTCAACATCGGTTTGGGCGAAGGCGCTCCAGAGGCCTGTGGCACGGGAGCTCC
CAGCGGCAAGGTGAGTCTGACCTCGTGGAACGTTGGCGAACGCCCCGCTGCGATGTTCCCACCAAGGAAGGACTAG

Upstream 100 bases:

>100_bases
ACGGCCACTTAACCTATGGCTTCAACTACATCGGCCCAACCGGAAACAACCGCGATGAGGTCACCAGAATTCGTCGCCGC
TCCCAGGAGGTGCAGTACTA

Downstream 100 bases:

>100_bases
GCGGATGCGCACCCATACTGGCAAAATTCCGGATCACTTTGTGCCTCGCATCTCCATGACGGAGGAGCAGCGGCGCGTGG
TGTTCATGCTGAATAGTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNRQGTEYIWFNNVETRPGVGYPRGWEDQDKWEGGWVRTNSGKLKPKAGG
RLKKLATIFHNPKLPTIDDYYEPWTYEYDKLLSAPAGQKTQPTARPVSQLDGRPIDKIEWSSNWDDNLGGSTTTLDQDPV
LEKMNLTVQKEIEDSFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDQCRGWRMCVSGCPYKKVYFNHKSGK
AEKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADRVAEVAATPDEKDLFEAQKTLFLDPHDPQVIADAQRNGIPHS
WIEAAQNSPIYDLIFKYEVALPLHPEYRTLPMVWYIPPLSPIVDEVTASGNDGEDHKILLTALSTMRIPLEYLAGLFTAG
DTRPVEKSLRRLAAMRSYMRDISLGREPQEEIAEAVGMTGKVVQEMYRILAIAKYDDRYVIPTASPETPRGISSLDPFGD
VDPARATEQLNIGLGEGAPEACGTGAPSGKVSLTSWNVGERPAAMFPPRKD

Sequences:

>Translated_531_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNRQGTEYIWFNNVETRPGVGYPRGWEDQDKWEGGWVRTNSGKLKPKAGG
RLKKLATIFHNPKLPTIDDYYEPWTYEYDKLLSAPAGQKTQPTARPVSQLDGRPIDKIEWSSNWDDNLGGSTTTLDQDPV
LEKMNLTVQKEIEDSFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDQCRGWRMCVSGCPYKKVYFNHKSGK
AEKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADRVAEVAATPDEKDLFEAQKTLFLDPHDPQVIADAQRNGIPHS
WIEAAQNSPIYDLIFKYEVALPLHPEYRTLPMVWYIPPLSPIVDEVTASGNDGEDHKILLTALSTMRIPLEYLAGLFTAG
DTRPVEKSLRRLAAMRSYMRDISLGREPQEEIAEAVGMTGKVVQEMYRILAIAKYDDRYVIPTASPETPRGISSLDPFGD
VDPARATEQLNIGLGEGAPEACGTGAPSGKVSLTSWNVGERPAAMFPPRKD
>Mature_531_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNRQGTEYIWFNNVETRPGVGYPRGWEDQDKWEGGWVRTNSGKLKPKAGG
RLKKLATIFHNPKLPTIDDYYEPWTYEYDKLLSAPAGQKTQPTARPVSQLDGRPIDKIEWSSNWDDNLGGSTTTLDQDPV
LEKMNLTVQKEIEDSFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDQCRGWRMCVSGCPYKKVYFNHKSGK
AEKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADRVAEVAATPDEKDLFEAQKTLFLDPHDPQVIADAQRNGIPHS
WIEAAQNSPIYDLIFKYEVALPLHPEYRTLPMVWYIPPLSPIVDEVTASGNDGEDHKILLTALSTMRIPLEYLAGLFTAG
DTRPVEKSLRRLAAMRSYMRDISLGREPQEEIAEAVGMTGKVVQEMYRILAIAKYDDRYVIPTASPETPRGISSLDPFGD
VDPARATEQLNIGLGEGAPEACGTGAPSGKVSLTSWNVGERPAAMFPPRKD

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=467, Percent_Identity=57.8158458244111, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1787478, Length=472, Percent_Identity=56.9915254237288, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=43.4343434343434, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=43.4343434343434, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789370, Length=137, Percent_Identity=35.036496350365, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787749, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI2367345, Length=175, Percent_Identity=27.4285714285714, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790326, Length=154, Percent_Identity=30.5194805194805, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI226510944, Length=115, Percent_Identity=34.7826086956522, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59362; Mature: 59362

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNRQGTEYIWFNNVETRPGVGYPRGWEDQD
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWEGGWVRTNSGKLKPKAGGRLKKLATIFHNPKLPTIDDYYEPWTYEYDKLLSAPAGQKT
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
QPTARPVSQLDGRPIDKIEWSSNWDDNLGGSTTTLDQDPVLEKMNLTVQKEIEDSFMFYL
CCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
PRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDQCRGWRMCVSGCPYKKVYFNHKSGK
HHHHHHHCCCHHHHCCCCCCHHHCCCCCEEEEECHHCCCHHHHHCCCCCEEEEEECCCCC
AEKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADRVAEVAATPDEKDLFEAQKTLF
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHCCCCHHHHHHHHHHEE
LDPHDPQVIADAQRNGIPHSWIEAAQNSPIYDLIFKYEVALPLHPEYRTLPMVWYIPPLS
ECCCCCCEEECHHHCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCH
PIVDEVTASGNDGEDHKILLTALSTMRIPLEYLAGLFTAGDTRPVEKSLRRLAAMRSYMR
HHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DISLGREPQEEIAEAVGMTGKVVQEMYRILAIAKYDDRYVIPTASPETPRGISSLDPFGD
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCCCCCCC
VDPARATEQLNIGLGEGAPEACGTGAPSGKVSLTSWNVGERPAAMFPPRKD
CCHHHHHHHEEEECCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNRQGTEYIWFNNVETRPGVGYPRGWEDQD
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWEGGWVRTNSGKLKPKAGGRLKKLATIFHNPKLPTIDDYYEPWTYEYDKLLSAPAGQKT
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
QPTARPVSQLDGRPIDKIEWSSNWDDNLGGSTTTLDQDPVLEKMNLTVQKEIEDSFMFYL
CCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
PRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDQCRGWRMCVSGCPYKKVYFNHKSGK
HHHHHHHCCCHHHHCCCCCCHHHCCCCCEEEEECHHCCCHHHHHCCCCCEEEEEECCCCC
AEKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADRVAEVAATPDEKDLFEAQKTLF
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHCCCCHHHHHHHHHHEE
LDPHDPQVIADAQRNGIPHSWIEAAQNSPIYDLIFKYEVALPLHPEYRTLPMVWYIPPLS
ECCCCCCEEECHHHCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCCH
PIVDEVTASGNDGEDHKILLTALSTMRIPLEYLAGLFTAGDTRPVEKSLRRLAAMRSYMR
HHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DISLGREPQEEIAEAVGMTGKVVQEMYRILAIAKYDDRYVIPTASPETPRGISSLDPFGD
HHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCCCCCCC
VDPARATEQLNIGLGEGAPEACGTGAPSGKVSLTSWNVGERPAAMFPPRKD
CCHHHHHHHEEEECCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]