Click here to switch to the map view.

The map label for this gene is mhpA [H]

Identifier: 145295300

GI number: 145295300

Start: 1355867

End: 1357459

Strand: Direct

Name: mhpA [H]

Synonym: cgR_1240

Alternate gene names: 145295300

Gene position: 1355867-1357459 (Clockwise)

Preceding gene: 145295298

Following gene: 145295301

Centisome position: 40.91

GC content: 52.79

Gene sequence:

>1593_bases
ATGTCACCCAATAACTTCGATACCGATGTCTGCATCGTCGGTGGAGGTCCTACCGGAACGCTCCTTGCAGTACTGCTCGG
CCAAAAAGGTCATCGCGTCACCATCCTGGAAAAGTGGCCAACATTCTACGAACGACCTCGTGCAGTCACCTTTGACCACG
AAATCGCGCGGATCCTTGGATACATTGGCATTGATTCTGAAAACGACGAAGCCATCGATTACCACTCCGACAGCTACGAC
TGGAAGAACGCAGCGGGGGAGACGCTTTTGGAAGTCGATTGGACCTCCATGACAGATTCCGGATGGCGCACCCGATACTG
GTTCTACCAGCCAGAACTTGAAAAGCGTCTGCGCGATCTGGCCCTGACCATGGATTTTGTGGATATTCGCTGTGGCTTCA
CCGCTGTCGGATTGTCCCAAGATGAAAACTCCGCCACCATTCACGGCATTGTCACTGATACCCCAGAGAACATTCCAGCA
GATGCTCAGCGCGAAGATATCCGAGCGAAGTATGTCATCGGTGCAGACGGAGCTAACAGTTTCGTGCGTAACTCCCTTGG
CTTAGAGATGAATGATCTTGGATACTTCTTCGACTGGCTGATTCTGGACCTCAAGCCAACTCAGGACATTGACTACGGAA
CAGATCACTGGCAACTGTGTGATCCCAAGCGCCCAACCACCATTGTTCCTGGAGGCCCCGGCCGCCGACGTTGGGAATTC
ATGGCGCTGCCTGGTGAAGATCTCAAGGAACTCGCTTCTGAAGAAAGCGCGTGGAAGCTACTTGAGCCATGGGATGTCAC
ACCTGACAAGGCTGTTCTCGAGCGCTCCGCAGTGTATCGATTCCAAGCTCGATGGGCCCAAGAATGGCGGTCCGGTAGGG
CTCTCATCGCAGGCGACGCCGCTCACCTCATGCCACCTTTCGCAGGTGAAGGCATGTGCGCGGGCTTGAGAGATTCACTG
GCGTTGGCATGGCGTCTTGATTTGGTGCTGAGCGGAAAATCAGATGATGCATTGCTAGACACCTACGGAGAAGAACGTCG
TGAACACGTCCACTACTACATTGATTTCTCCATGGACCTGGGCAATGTCATCTGCATTACTGATGAAGATGAAGCACGTT
TGCGCGATGAGCGCATGATTAAAGAGCTTGAAGCACAAGACGGGGTCCCTGTTAATACCGATGTCGCGCACTTGGGACCG
GGAATTTGGGATAAAGATTCTTCCCATGGTGGCGAGCTAGCGAAGCAGGGCATAGTGGAATACCAAGGTCGAAAGGCGCG
TTTCGACGACGCAGTCGGCCGTGGCTGGGCAGTTTTAGGCCTCAACACTGATCCACGAGAAGTGCTTGATGAGGATTCGC
TTGTGGCACTTGACGCCATCGGTGCAATCGTCGAATCAGTAGGTGATGCAACTTCCGCAGTTTTAGATGTTGAAGGTCTT
TACACTCGCTGGCTCAAGGAAGCCGGGGCAACATTTATTATTACCCGCCCCGATTTCTACGTCTATTCCACAGCAGTGGA
CGCTGAACAACTTCAAACACAGATTAAGCAGCTATCGGATCTACTTCACCTCAACTCAGTTGTCGGAGCATAG

Upstream 100 bases:

>100_bases
GGTATTGTAAATTCTGAATTCTTGTTCATTTGTGAATCATGATTCAGAATGTGATCTAGATAATGTTGTTCAGTTCACTA
TTCAAGAAGGGTTAGATCCC

Downstream 100 bases:

>100_bases
GAGCTAAAAATGTCTTTACAGTTCGATCATGAAACCCTCGGCCAACGAGTTCTGTTCGGTTCAGGTGAGGCGGCGCAAAA
TCTCGCCGCTGAAATTAGCC

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: tetrachlorohydroquinone; NADP+; chloride

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSPNNFDTDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILGYIGIDSENDEAIDYHSDSYD
WKNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDLALTMDFVDIRCGFTAVGLSQDENSATIHGIVTDTPENIPA
DAQREDIRAKYVIGADGANSFVRNSLGLEMNDLGYFFDWLILDLKPTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEF
MALPGEDLKELASEESAWKLLEPWDVTPDKAVLERSAVYRFQARWAQEWRSGRALIAGDAAHLMPPFAGEGMCAGLRDSL
ALAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDLGNVICITDEDEARLRDERMIKELEAQDGVPVNTDVAHLGP
GIWDKDSSHGGELAKQGIVEYQGRKARFDDAVGRGWAVLGLNTDPREVLDEDSLVALDAIGAIVESVGDATSAVLDVEGL
YTRWLKEAGATFIITRPDFYVYSTAVDAEQLQTQIKQLSDLLHLNSVVGA

Sequences:

>Translated_530_residues
MSPNNFDTDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILGYIGIDSENDEAIDYHSDSYD
WKNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDLALTMDFVDIRCGFTAVGLSQDENSATIHGIVTDTPENIPA
DAQREDIRAKYVIGADGANSFVRNSLGLEMNDLGYFFDWLILDLKPTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEF
MALPGEDLKELASEESAWKLLEPWDVTPDKAVLERSAVYRFQARWAQEWRSGRALIAGDAAHLMPPFAGEGMCAGLRDSL
ALAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDLGNVICITDEDEARLRDERMIKELEAQDGVPVNTDVAHLGP
GIWDKDSSHGGELAKQGIVEYQGRKARFDDAVGRGWAVLGLNTDPREVLDEDSLVALDAIGAIVESVGDATSAVLDVEGL
YTRWLKEAGATFIITRPDFYVYSTAVDAEQLQTQIKQLSDLLHLNSVVGA
>Mature_529_residues
SPNNFDTDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILGYIGIDSENDEAIDYHSDSYDW
KNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDLALTMDFVDIRCGFTAVGLSQDENSATIHGIVTDTPENIPAD
AQREDIRAKYVIGADGANSFVRNSLGLEMNDLGYFFDWLILDLKPTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEFM
ALPGEDLKELASEESAWKLLEPWDVTPDKAVLERSAVYRFQARWAQEWRSGRALIAGDAAHLMPPFAGEGMCAGLRDSLA
LAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDLGNVICITDEDEARLRDERMIKELEAQDGVPVNTDVAHLGPG
IWDKDSSHGGELAKQGIVEYQGRKARFDDAVGRGWAVLGLNTDPREVLDEDSLVALDAIGAIVESVGDATSAVLDVEGLY
TRWLKEAGATFIITRPDFYVYSTAVDAEQLQTQIKQLSDLLHLNSVVGA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=549, Percent_Identity=30.0546448087432, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.50

Molecular weight: Translated: 59242; Mature: 59111

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNNFDTDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILG
CCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHCCCEEEEHHHHHHHHH
YIGIDSENDEAIDYHSDSYDWKNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDL
HHCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECHHHHHHHHHH
ALTMDFVDIRCGFTAVGLSQDENSATIHGIVTDTPENIPADAQREDIRAKYVIGADGANS
HHHHEEEEEECCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHH
FVRNSLGLEMNDLGYFFDWLILDLKPTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEF
HHHHHCCCEEHHHHHEEEEEEEECCCCCCCCCCCCCCEECCCCCCCEEECCCCCCCEEEE
MALPGEDLKELASEESAWKLLEPWDVTPDKAVLERSAVYRFQARWAQEWRSGRALIAGDA
EECCCHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCH
AHLMPPFAGEGMCAGLRDSLALAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDL
HHHCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHCEEEEEEEEEECC
GNVICITDEDEARLRDERMIKELEAQDGVPVNTDVAHLGPGIWDKDSSHGGELAKQGIVE
CCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHH
YQGRKARFDDAVGRGWAVLGLNTDPREVLDEDSLVALDAIGAIVESVGDATSAVLDVEGL
HCCCCCCCCHHCCCCEEEEECCCCHHHHHCCCCEEHHHHHHHHHHHHCCHHHHEEEHHHH
YTRWLKEAGATFIITRPDFYVYSTAVDAEQLQTQIKQLSDLLHLNSVVGA
HHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPNNFDTDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILG
CCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHCCCEEEEHHHHHHHHH
YIGIDSENDEAIDYHSDSYDWKNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDL
HHCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECHHHHHHHHHH
ALTMDFVDIRCGFTAVGLSQDENSATIHGIVTDTPENIPADAQREDIRAKYVIGADGANS
HHHHEEEEEECCEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHH
FVRNSLGLEMNDLGYFFDWLILDLKPTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEF
HHHHHCCCEEHHHHHEEEEEEEECCCCCCCCCCCCCCEECCCCCCCEEECCCCCCCEEEE
MALPGEDLKELASEESAWKLLEPWDVTPDKAVLERSAVYRFQARWAQEWRSGRALIAGDA
EECCCHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCH
AHLMPPFAGEGMCAGLRDSLALAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDL
HHHCCCCCCCCCCCCHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHCEEEEEEEEEECC
GNVICITDEDEARLRDERMIKELEAQDGVPVNTDVAHLGPGIWDKDSSHGGELAKQGIVE
CCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHH
YQGRKARFDDAVGRGWAVLGLNTDPREVLDEDSLVALDAIGAIVESVGDATSAVLDVEGL
HCCCCCCCCHHCCCCEEEEECCCCHHHHHCCCCEEHHHHHHHHHHHHCCHHHHEEEHHHH
YTRWLKEAGATFIITRPDFYVYSTAVDAEQLQTQIKQLSDLLHLNSVVGA
HHHHHHHCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pentachlorophenol; NADPH; H+; O2

Specific reaction: pentachlorophenol + NADPH + H+ + O2 = tetrachlorohydroquinone + NADP+ + chloride

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA