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The map label for this gene is sigW [H]

Identifier: 145295263

GI number: 145295263

Start: 1308233

End: 1308874

Strand: Direct

Name: sigW [H]

Synonym: cgR_1204

Alternate gene names: 145295263

Gene position: 1308233-1308874 (Clockwise)

Preceding gene: 145295261

Following gene: 145295264

Centisome position: 39.47

GC content: 55.14

Gene sequence:

>642_bases
ATGAAAAAGAAGTCCCGAGATGACGCACCCGTCGTAATCGAAACCGTTCAAGCGGAACATGCTGAAGAACTCACGGGCAC
TGCAGCATTCGATGCTGGACAGGCAGACATGCCAACATGGGGCGAGCTAGTCGCAGAACATGCAGATAGCGTTTACCGCC
TCGCGTACCGTCTTTCCGGCAACCAGCACGATGCCGAAGACCTGACCCAAGAAACATTCATGCGTGTCTTCCGCTCGTTG
AAGAGCTACCAGCCAGGCACCTTTGAGGGCTGGCTGCACCGCATCACCACCAACTTGTTCCTTGATATGGTTCGCCACCG
CGGCAAGATCCGCATGGAGGCGCTGCCTGAAGATTATGAGCGCGTTCCGGGCAATGACATCACCCCAGAGCAGGCCTACA
CCGAAGCGAATCTTGATCCTGCATTGCAGGCAGCCCTCGATGAGTTGAGCCCAGACTTCCGCGTGGCCGTGATCCTCTGT
GATGTTGTTGGTATGAGCTATGACGAAATCGCAGAAACCCTCGGAGTGAAAATGGGTACCGTGCGTTCCCGTATTCACCG
TGGACGCAGCCAGCTTCGTGCAAGTTTGGAAGCTGCAGCAATGACCAGCGAGGAAGTTTCTTTGTTGGTTCCAACCCACT
AA

Upstream 100 bases:

>100_bases
AAGTACCTACACGCCTTAAGTGCAAATGAATTCACAATTGCCAGAAGATGCACAGGATGTAATCTAGATTTCCCAAGTTC
AGTGGGGCAAAATGACTTAT

Downstream 100 bases:

>100_bases
AGTTGGTGTGTTTTCTGACACGACAAACGCAAATGTCGTGTCATTTTTGCAGCTCAGTGCATTATTTTGGGGTTCGTGGT
GCGGACAGGGAACTTATCGC

Product: RNA polymerase sigma factor SigE

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKKKSRDDAPVVIETVQAEHAEELTGTAAFDAGQADMPTWGELVAEHADSVYRLAYRLSGNQHDAEDLTQETFMRVFRSL
KSYQPGTFEGWLHRITTNLFLDMVRHRGKIRMEALPEDYERVPGNDITPEQAYTEANLDPALQAALDELSPDFRVAVILC
DVVGMSYDEIAETLGVKMGTVRSRIHRGRSQLRASLEAAAMTSEEVSLLVPTH

Sequences:

>Translated_213_residues
MKKKSRDDAPVVIETVQAEHAEELTGTAAFDAGQADMPTWGELVAEHADSVYRLAYRLSGNQHDAEDLTQETFMRVFRSL
KSYQPGTFEGWLHRITTNLFLDMVRHRGKIRMEALPEDYERVPGNDITPEQAYTEANLDPALQAALDELSPDFRVAVILC
DVVGMSYDEIAETLGVKMGTVRSRIHRGRSQLRASLEAAAMTSEEVSLLVPTH
>Mature_213_residues
MKKKSRDDAPVVIETVQAEHAEELTGTAAFDAGQADMPTWGELVAEHADSVYRLAYRLSGNQHDAEDLTQETFMRVFRSL
KSYQPGTFEGWLHRITTNLFLDMVRHRGKIRMEALPEDYERVPGNDITPEQAYTEANLDPALQAALDELSPDFRVAVILC
DVVGMSYDEIAETLGVKMGTVRSRIHRGRSQLRASLEAAAMTSEEVSLLVPTH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=27.7486910994764, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23820; Mature: 23820

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKSRDDAPVVIETVQAEHAEELTGTAAFDAGQADMPTWGELVAEHADSVYRLAYRLSG
CCCCCCCCCCEEEHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NQHDAEDLTQETFMRVFRSLKSYQPGTFEGWLHRITTNLFLDMVRHRGKIRMEALPEDYE
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
RVPGNDITPEQAYTEANLDPALQAALDELSPDFRVAVILCDVVGMSYDEIAETLGVKMGT
HCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHH
VRSRIHRGRSQLRASLEAAAMTSEEVSLLVPTH
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure
MKKKSRDDAPVVIETVQAEHAEELTGTAAFDAGQADMPTWGELVAEHADSVYRLAYRLSG
CCCCCCCCCCEEEHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
NQHDAEDLTQETFMRVFRSLKSYQPGTFEGWLHRITTNLFLDMVRHRGKIRMEALPEDYE
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHH
RVPGNDITPEQAYTEANLDPALQAALDELSPDFRVAVILCDVVGMSYDEIAETLGVKMGT
HCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHH
VRSRIHRGRSQLRASLEAAAMTSEEVSLLVPTH
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]