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The map label for this gene is folP2 [H]

Identifier: 145295253

GI number: 145295253

Start: 1300182

End: 1300799

Strand: Direct

Name: folP2 [H]

Synonym: cgR_1194

Alternate gene names: 145295253

Gene position: 1300182-1300799 (Clockwise)

Preceding gene: 145295252

Following gene: 145295254

Centisome position: 39.23

GC content: 56.8

Gene sequence:

>618_bases
ATGAGTTCTTTGCCGGTCATCATGGCCATCGTCAACCGCACCCCGGATTCTTTCTATGACAAGGGTGCGACATTTGAGGA
CACCGCTGCGCTAAACAGGGCAACGGAGGTCATTGAACAAGGCGCCGGCATTGTCGATATCGGTGGGGTGAAAGCCGGCC
CGGGGGATTTCGTGTCGGCGGAGGAAGAGATCGACCGCGTGGTGCCAATCATCGCTGCGGTGCGAGAACGTTTTCCTGAC
ATTGATATTTCTGTTGATACCTGGCGGGCGTCGGTGGCTGATGTCGCAGTGGCGCATGGAGCAACGCTGATCAATGACAC
TTGGGCCGGCCATGATCATGAGTTGGTGCAGGTAGCAGGGCAGCACAAGGTGGGTTATGTCTGCTCGCACACCGGCGGGG
TGATTCCAAGAACGCGACCATATCGGGTGCATTTCGATGACATCGTGGCCGATGTAATTACGGAGACCACCAAATTGGCA
GAGCAAGCTGTTCGTGCCGGGGTGCCAGAGGAACGGGTGTTTATTGATCCCACCCATGATTCGGGAAAAACACCTTCCAC
GGACTGGAGCTTTTACGACGGATCGATGAGGTGGTTGCCACGGGCTGGCCGGTGCTGA

Upstream 100 bases:

>100_bases
CACTGATGACCTGGATCAGGCCGTCAAATTCATCGTCGATGCACACGCTGGATTGGACGTAGCGCGTCGCCACAATTAAG
CAGTGGCTACATTAGGTGTT

Downstream 100 bases:

>100_bases
TGGCCTTGAGTAATAAGGATTTCATTGGGGAAACTTTGGAAAGGGGCGTCGATAAGCGTGTTGCTGGCACGCTTGCGGCC
ACTGCCTGGGCCGCGGCGCG

Product: hypothetical protein

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSSLPVIMAIVNRTPDSFYDKGATFEDTAALNRATEVIEQGAGIVDIGGVKAGPGDFVSAEEEIDRVVPIIAAVRERFPD
IDISVDTWRASVADVAVAHGATLINDTWAGHDHELVQVAGQHKVGYVCSHTGGVIPRTRPYRVHFDDIVADVITETTKLA
EQAVRAGVPEERVFIDPTHDSGKTPSTDWSFYDGSMRWLPRAGRC

Sequences:

>Translated_205_residues
MSSLPVIMAIVNRTPDSFYDKGATFEDTAALNRATEVIEQGAGIVDIGGVKAGPGDFVSAEEEIDRVVPIIAAVRERFPD
IDISVDTWRASVADVAVAHGATLINDTWAGHDHELVQVAGQHKVGYVCSHTGGVIPRTRPYRVHFDDIVADVITETTKLA
EQAVRAGVPEERVFIDPTHDSGKTPSTDWSFYDGSMRWLPRAGRC
>Mature_204_residues
SSLPVIMAIVNRTPDSFYDKGATFEDTAALNRATEVIEQGAGIVDIGGVKAGPGDFVSAEEEIDRVVPIIAAVRERFPDI
DISVDTWRASVADVAVAHGATLINDTWAGHDHELVQVAGQHKVGYVCSHTGGVIPRTRPYRVHFDDIVADVITETTKLAE
QAVRAGVPEERVFIDPTHDSGKTPSTDWSFYDGSMRWLPRAGRC

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=191, Percent_Identity=32.9842931937173, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324073, Length=142, Percent_Identity=37.3239436619718, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 22256; Mature: 22125

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPVIMAIVNRTPDSFYDKGATFEDTAALNRATEVIEQGAGIVDIGGVKAGPGDFVSA
CCCCCEEHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
EEEIDRVVPIIAAVRERFPDIDISVDTWRASVADVAVAHGATLINDTWAGHDHELVQVAG
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHC
QHKVGYVCSHTGGVIPRTRPYRVHFDDIVADVITETTKLAEQAVRAGVPEERVFIDPTHD
CCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
SGKTPSTDWSFYDGSMRWLPRAGRC
CCCCCCCCCEEECCCCEECCCCCCC
>Mature Secondary Structure 
SSLPVIMAIVNRTPDSFYDKGATFEDTAALNRATEVIEQGAGIVDIGGVKAGPGDFVSA
CCCCEEHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
EEEIDRVVPIIAAVRERFPDIDISVDTWRASVADVAVAHGATLINDTWAGHDHELVQVAG
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHC
QHKVGYVCSHTGGVIPRTRPYRVHFDDIVADVITETTKLAEQAVRAGVPEERVFIDPTHD
CCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
SGKTPSTDWSFYDGSMRWLPRAGRC
CCCCCCCCCEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]