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The map label for this gene is glyA [H]

Identifier: 145295144

GI number: 145295144

Start: 1199130

End: 1200434

Strand: Direct

Name: glyA [H]

Synonym: cgR_1087

Alternate gene names: 145295144

Gene position: 1199130-1200434 (Clockwise)

Preceding gene: 145295142

Following gene: 145295145

Centisome position: 36.18

GC content: 53.95

Gene sequence:

>1305_bases
ATGACCGATGCCCACCAAGCGGACGATGTCCGTTACCAGCCACTGAACGAGCTTGATCCTGAGGTGGCTGCTGCCATCGC
TGGGGAACTTGCCCGTCAACGCGATACATTAGAGATGATCGCGTCTGAGAACTTCGTTCCCCGTTCTGTTTTGCAGGCGC
AGGGTTCTGTTCTTACCAATAAGTATGCCGAGGGTTACCCTGGCCGCCGTTACTACGGTGGTTGCGAACAAGTTGACATC
ATTGAGGATCTTGCACGTGATCGTGCGAAGGCTCTCTTCGGTGCAGAGTTCGCCAATGTTCAGCCTCACTCCGGCGCGCA
GGCTAATGCTGCTGTGCTGATGACTTTGGCTGAGCCAGGCGACAAGATCATGGGTCTGTCTTTGGCTCATGGTGGTCACT
TGACCCACGGAATGAAGTTGAACTTCTCCGGAAAGCTGTACGAGGTTGTTGCGTACGGTGTTGATCCTGAGACCATGCGT
GTTGATATGGATCAGGTTCGTGAGATTGCTCTGAAGGAGCAGCCAAAGGTAATTATCGCTGGCTGGTCTGCATACCCTCG
CCACCTTGATTTCGAGGCTTTCCAGTCTATTGCTGCGGAAGTTGGCGCGAAGCTGTGGGTCGATATGGCTCACTTCGCTG
GTCTTGTTGCTGCTGGTTTGCACCCAAGCCCAGTTCCTTACTCTGATGTTGTTTCTTCCACTGTCCACAAGACTTTGGGT
GGACCTCGTTCCGGCATCATTCTGGCTAAGCAGGAGTACGCGAAGAAGCTGAACTCTTCCGTATTCCCAGGTCAGCAGGG
TGGTCCTTTGATGCACGCAGTTGCTGCGAAGGCTACTTCTTTGAAGATTGCTGGCACTGAGCAGTTCCGTGACCGTCAGG
CTCGCACGTTGGAGGGTGCTCGCATTCTTGCCGAGCGTCTGACTGCTTCTGATGCGAAGGCCGCTGGCGTGGATGTCTTG
ACCGGTGGCACTGATGTGCACTTGGTTTTGGCTGATCTGCGTAACTCCCAGATGGATGGCCAACAGGCGGAAGATCTGCT
GCACGAGGTTGGTATCACTGTGAACCGTAACGCGGTTCCTTTCGATCCTCGTCCACCAATGGTTACTTCTGGTCTGCGTA
TTGGTACTCCTGCGCTGGCTACCCGTGGTTTCGATATTCCTGCATTCACTGAGGTTGCAGACATCATCGGTACTGCTTTG
GCTAATGGTAAGTCCGCAGACATTGAGTCCCTGCGTGGCCGTGTAGCAAAGCTTGCTGCAGATTACCCACTGTATGAGGG
CTTGGAAGACTGGACCATCGTCTAA

Upstream 100 bases:

>100_bases
TTTTATATATGGGTATCGGCGGTCTATGCTTGTGGGCGTACCTGTCCCGCGAGTGAGGTCTTACGCGCGGGATTCGTCTT
GTGAAAGGTTAGCTGACCTG

Downstream 100 bases:

>100_bases
GCTTTTCTTTGAGTTTTCATATGTAGAAGGCATCGTCGGCTTCGGCCTGGCGGTGCTTTTCTCGTTGTTTTGTGGTTTTG
TCAGAGGATGTCATGCGCGT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTDAHQADDVRYQPLNELDPEVAAAIAGELARQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGYPGRRYYGGCEQVDI
IEDLARDRAKALFGAEFANVQPHSGAQANAAVLMTLAEPGDKIMGLSLAHGGHLTHGMKLNFSGKLYEVVAYGVDPETMR
VDMDQVREIALKEQPKVIIAGWSAYPRHLDFEAFQSIAAEVGAKLWVDMAHFAGLVAAGLHPSPVPYSDVVSSTVHKTLG
GPRSGIILAKQEYAKKLNSSVFPGQQGGPLMHAVAAKATSLKIAGTEQFRDRQARTLEGARILAERLTASDAKAAGVDVL
TGGTDVHLVLADLRNSQMDGQQAEDLLHEVGITVNRNAVPFDPRPPMVTSGLRIGTPALATRGFDIPAFTEVADIIGTAL
ANGKSADIESLRGRVAKLAADYPLYEGLEDWTIV

Sequences:

>Translated_434_residues
MTDAHQADDVRYQPLNELDPEVAAAIAGELARQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGYPGRRYYGGCEQVDI
IEDLARDRAKALFGAEFANVQPHSGAQANAAVLMTLAEPGDKIMGLSLAHGGHLTHGMKLNFSGKLYEVVAYGVDPETMR
VDMDQVREIALKEQPKVIIAGWSAYPRHLDFEAFQSIAAEVGAKLWVDMAHFAGLVAAGLHPSPVPYSDVVSSTVHKTLG
GPRSGIILAKQEYAKKLNSSVFPGQQGGPLMHAVAAKATSLKIAGTEQFRDRQARTLEGARILAERLTASDAKAAGVDVL
TGGTDVHLVLADLRNSQMDGQQAEDLLHEVGITVNRNAVPFDPRPPMVTSGLRIGTPALATRGFDIPAFTEVADIIGTAL
ANGKSADIESLRGRVAKLAADYPLYEGLEDWTIV
>Mature_433_residues
TDAHQADDVRYQPLNELDPEVAAAIAGELARQRDTLEMIASENFVPRSVLQAQGSVLTNKYAEGYPGRRYYGGCEQVDII
EDLARDRAKALFGAEFANVQPHSGAQANAAVLMTLAEPGDKIMGLSLAHGGHLTHGMKLNFSGKLYEVVAYGVDPETMRV
DMDQVREIALKEQPKVIIAGWSAYPRHLDFEAFQSIAAEVGAKLWVDMAHFAGLVAAGLHPSPVPYSDVVSSTVHKTLGG
PRSGIILAKQEYAKKLNSSVFPGQQGGPLMHAVAAKATSLKIAGTEQFRDRQARTLEGARILAERLTASDAKAAGVDVLT
GGTDVHLVLADLRNSQMDGQQAEDLLHEVGITVNRNAVPFDPRPPMVTSGLRIGTPALATRGFDIPAFTEVADIIGTALA
NGKSADIESLRGRVAKLAADYPLYEGLEDWTIV

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=458, Percent_Identity=40.3930131004367, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI261862350, Length=458, Percent_Identity=40.3930131004367, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI261862348, Length=458, Percent_Identity=40.3930131004367, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI19923315, Length=458, Percent_Identity=40.3930131004367, Blast_Score=308, Evalue=8e-84,
Organism=Homo sapiens, GI22547186, Length=413, Percent_Identity=44.3099273607748, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=40.1766004415011, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI22547189, Length=398, Percent_Identity=42.713567839196, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1788902, Length=419, Percent_Identity=51.0739856801909, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25144732, Length=422, Percent_Identity=43.1279620853081, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25144729, Length=422, Percent_Identity=43.1279620853081, Blast_Score=318, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6319739, Length=463, Percent_Identity=38.4449244060475, Blast_Score=313, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6323087, Length=424, Percent_Identity=39.3867924528302, Blast_Score=300, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24640005, Length=463, Percent_Identity=42.7645788336933, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI221329721, Length=463, Percent_Identity=42.7645788336933, Blast_Score=341, Evalue=6e-94,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46541; Mature: 46410

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAHQADDVRYQPLNELDPEVAAAIAGELARQRDTLEMIASENFVPRSVLQAQGSVLTN
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
KYAEGYPGRRYYGGCEQVDIIEDLARDRAKALFGAEFANVQPHSGAQANAAVLMTLAEPG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
DKIMGLSLAHGGHLTHGMKLNFSGKLYEVVAYGVDPETMRVDMDQVREIALKEQPKVIIA
CCEEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEECHHHHHHHHHCCCCCEEEE
GWSAYPRHLDFEAFQSIAAEVGAKLWVDMAHFAGLVAAGLHPSPVPYSDVVSSTVHKTLG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
GPRSGIILAKQEYAKKLNSSVFPGQQGGPLMHAVAAKATSLKIAGTEQFRDRQARTLEGA
CCCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHH
RILAERLTASDAKAAGVDVLTGGTDVHLVLADLRNSQMDGQQAEDLLHEVGITVNRNAVP
HHHHHHHHCCCCCCCCCEEEECCCCEEEEEEHHCCCCCCCHHHHHHHHHCCCEECCCCCC
FDPRPPMVTSGLRIGTPALATRGFDIPAFTEVADIIGTALANGKSADIESLRGRVAKLAA
CCCCCCCEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DYPLYEGLEDWTIV
CCHHHCCCCCCCCC
>Mature Secondary Structure 
TDAHQADDVRYQPLNELDPEVAAAIAGELARQRDTLEMIASENFVPRSVLQAQGSVLTN
CCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
KYAEGYPGRRYYGGCEQVDIIEDLARDRAKALFGAEFANVQPHSGAQANAAVLMTLAEPG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
DKIMGLSLAHGGHLTHGMKLNFSGKLYEVVAYGVDPETMRVDMDQVREIALKEQPKVIIA
CCEEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCHHEEECHHHHHHHHHCCCCCEEEE
GWSAYPRHLDFEAFQSIAAEVGAKLWVDMAHFAGLVAAGLHPSPVPYSDVVSSTVHKTLG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
GPRSGIILAKQEYAKKLNSSVFPGQQGGPLMHAVAAKATSLKIAGTEQFRDRQARTLEGA
CCCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHH
RILAERLTASDAKAAGVDVLTGGTDVHLVLADLRNSQMDGQQAEDLLHEVGITVNRNAVP
HHHHHHHHCCCCCCCCCEEEECCCCEEEEEEHHCCCCCCCHHHHHHHHHCCCEECCCCCC
FDPRPPMVTSGLRIGTPALATRGFDIPAFTEVADIIGTALANGKSADIESLRGRVAKLAA
CCCCCCCEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DYPLYEGLEDWTIV
CCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]