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The map label for this gene is pcrA [H]

Identifier: 145295025

GI number: 145295025

Start: 1075776

End: 1078067

Strand: Direct

Name: pcrA [H]

Synonym: cgR_0969

Alternate gene names: 145295025

Gene position: 1075776-1078067 (Clockwise)

Preceding gene: 145295024

Following gene: 145295030

Centisome position: 32.46

GC content: 55.45

Gene sequence:

>2292_bases
ATGAATACTTCTCCTTTTACCCCAGGTTCCCCCGATCTCATCGATGGTTTGAATGAGCAACAGCGTGCTGCTGTGGAGCA
TATTGGTTCTCCGCTGCTGATTGTCGCTGGTGCTGGTTCAGGCAAGACTGCTGTGTTGACCAGGCGTATTGCTTATTTGA
TGCGTTACCGTGGTGTGCATCCGCAGCAAATTTTGGCCATTACCTTTACCAATAAGGCTGCCGCTGAGATGCGTGAGCGT
GTCAGTCAGCTGGTGGGCCCGGTTGCAGAGCGCATGTGGGTGGCTACGTTCCACTCGGTGTGTGTGCGTATTTTGCGTCA
GCAGGCGCAGTTGGTGGAGGGGCTGAACACTAACTTCACTATTTATGATTCGGATGATTCGAGGCGTTTGCTCACGATGA
TCGCCAAGGATCTGGAGTTGGATATTAAGAAGTTCTCGGCGCGTACGTTGCTGGGTGCTATTTCTAATTTGAAAAATGAG
TTGGTTACTCCCCAGGAAGCTCTTGCGGATGCTGAACGCACGCACAACCCTTATGAAACAGTTGTGGCTAGGGCGTTTTC
GGAGTATCAGAGCAGGCTTCGCCGTGCCAACGCTGTGGATTTTGATGATCTGATTGGGGAGACTGTTCGGATTTTCCGGG
AGCATCCACCTGTTGCGGAGTATTACCGCAGACGTTTCCGTCACGTGCTGATCGATGAGTATCAGGACACCAACCACGCC
CAGTATGAGCTGATTTCTACACTTGTGGGCAAGCCTGACCAGGATCCGTCTGAGCTGTGTGTGGTGGGTGATTCGGATCA
GTCTATTTATGCTTTCCGTGGCGCCACGATCCGCAACATCGAAGAGTTTGAGCGCGATTTCTCCAACGCCCGCACCATTT
TGCTGGAGCAGAATTACCGTTCCACCCAGACGATTCTTTCTGCTGCCAACGCGGTGATTTCTCAAAATGAGAACCGTCGA
CCTAAAAACCTGTGGACTGCGCTGGGGGAGGGCGAGCAGATCATTGGTTATGTTGCCGACAATGAGCACGATGAAGCCCG
TTTTATTGCTAGTGAGATCGACAATTTAGTTGACCACGGCATGAACTATTCCGACATCGCGATCATGTACCGCACGAACA
ATTCCTCGCGCGCACTAGAGGATGTCTTCATGCGCACCGGCGTCCCCTACAAAGTAGTCGGCGGCACGAAGTTCTACGAA
CGCAAAGAAATCCGTGACATCATCGCTTACCTGCGCGTTTTGGAAAACCCCGATGACACCGTCAACCTCCGCCGCATCAT
CAACACCCCCAAGCGCGGCATCGGCGATCGCGCGCAGGCGTTCATCGCGCTGCACAGCGAGAACAATCAGATCAGCTTCG
GGCAAGCGCTTCTCGACGCCGGCCTCGGCAAAATCGACCTGCTCGGCGCACGCGGCAAGAATGCGGTCATCAAGTTCAAT
GAGCTTTTCGACGCCCTCCGCACCGAACTTCCCACCATGGTCAATGAGGTCACCGGCCTGCCAGATATCGGCCAAGTCAT
CAGCCGTATCCTCGATATCACCGGCTACAAAGCAGAACTTGAGGCCTCCAACGATCCACAAGATGGCGCACGCCTAGACA
ACCTGAACGAGCTTGTCTCCGTGGCCCGCGAGTTCTCATCCGACGCCGCAAACCGCATGGTTAATGAAGTTCCAGAAGGC
GAAGCCCAACCGGGCAGTTTACAGGCATTCTTGGAGCGCGTCTCCCTGGTTGCCGACGCCGACCAAATCCCCGATTCCGA
CAACGGCGTAGTCACCCTCATGACCCTGCATACCGCCAAGGGCCTTGAATTCCCCATCGTGTTCCTCACAGGCTGGGAAG
ACGGACAGTTCCCACACCTGCGTTCCCTTGGTGATGCCAAAGAACTTGCCGAGGAACGCCGCCTCGCCTACGTGGGTATC
ACCCGCGCCCGCAAGCGCCTCTACATGACCAGAGCCATGCTGCGCAGCTCCTGGGGCAACCCGGTGACCAACCCACCGTC
ACGTTTCCTCCAAGAAGTGCCCGCAGAACTCATCGATTGGAGGCGCGAAGAACCCCAGATGTCTTCCGCCTGGGCTCCTC
GACCCACCCGAAGCATCCCCACCAAAACTCGCACCAACAATAAGCAGTTGGATCTGTCGGTGGGGGATCGCGTCAATCAC
GACAAGTACGGCTTGGGAACCGTGCTCTCTTCAGATGGCAGCGGCCCCCGAGCCACCGTCACCATCGATTTCGGTTCCTC
CGGCAAGGTTAGATTGATGCTTCTTGGTGGCGTCCCGATGGAGAAGCTATAG

Upstream 100 bases:

>100_bases
CTGATCTCGAAGTTGTCACCTGCATTAGTCATGCACACTATTCTGCCACGTCTGGCTGTCGACTATGCGTGTCCACCCCA
CCTAGTAGGTTATTGGGGTA

Downstream 100 bases:

>100_bases
CCCCAATCACGGCGCACACCAGCGGCGCCACAACGAGGAGGCTTACCAACAGAGGCCACGGCAGCATCATGTGTTCAAGC
GTGCCGTATTCCAAGATTGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MNTSPFTPGSPDLIDGLNEQQRAAVEHIGSPLLIVAGAGSGKTAVLTRRIAYLMRYRGVHPQQILAITFTNKAAAEMRER
VSQLVGPVAERMWVATFHSVCVRILRQQAQLVEGLNTNFTIYDSDDSRRLLTMIAKDLELDIKKFSARTLLGAISNLKNE
LVTPQEALADAERTHNPYETVVARAFSEYQSRLRRANAVDFDDLIGETVRIFREHPPVAEYYRRRFRHVLIDEYQDTNHA
QYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFERDFSNARTILLEQNYRSTQTILSAANAVISQNENRR
PKNLWTALGEGEQIIGYVADNEHDEARFIASEIDNLVDHGMNYSDIAIMYRTNNSSRALEDVFMRTGVPYKVVGGTKFYE
RKEIRDIIAYLRVLENPDDTVNLRRIINTPKRGIGDRAQAFIALHSENNQISFGQALLDAGLGKIDLLGARGKNAVIKFN
ELFDALRTELPTMVNEVTGLPDIGQVISRILDITGYKAELEASNDPQDGARLDNLNELVSVAREFSSDAANRMVNEVPEG
EAQPGSLQAFLERVSLVADADQIPDSDNGVVTLMTLHTAKGLEFPIVFLTGWEDGQFPHLRSLGDAKELAEERRLAYVGI
TRARKRLYMTRAMLRSSWGNPVTNPPSRFLQEVPAELIDWRREEPQMSSAWAPRPTRSIPTKTRTNNKQLDLSVGDRVNH
DKYGLGTVLSSDGSGPRATVTIDFGSSGKVRLMLLGGVPMEKL

Sequences:

>Translated_763_residues
MNTSPFTPGSPDLIDGLNEQQRAAVEHIGSPLLIVAGAGSGKTAVLTRRIAYLMRYRGVHPQQILAITFTNKAAAEMRER
VSQLVGPVAERMWVATFHSVCVRILRQQAQLVEGLNTNFTIYDSDDSRRLLTMIAKDLELDIKKFSARTLLGAISNLKNE
LVTPQEALADAERTHNPYETVVARAFSEYQSRLRRANAVDFDDLIGETVRIFREHPPVAEYYRRRFRHVLIDEYQDTNHA
QYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFERDFSNARTILLEQNYRSTQTILSAANAVISQNENRR
PKNLWTALGEGEQIIGYVADNEHDEARFIASEIDNLVDHGMNYSDIAIMYRTNNSSRALEDVFMRTGVPYKVVGGTKFYE
RKEIRDIIAYLRVLENPDDTVNLRRIINTPKRGIGDRAQAFIALHSENNQISFGQALLDAGLGKIDLLGARGKNAVIKFN
ELFDALRTELPTMVNEVTGLPDIGQVISRILDITGYKAELEASNDPQDGARLDNLNELVSVAREFSSDAANRMVNEVPEG
EAQPGSLQAFLERVSLVADADQIPDSDNGVVTLMTLHTAKGLEFPIVFLTGWEDGQFPHLRSLGDAKELAEERRLAYVGI
TRARKRLYMTRAMLRSSWGNPVTNPPSRFLQEVPAELIDWRREEPQMSSAWAPRPTRSIPTKTRTNNKQLDLSVGDRVNH
DKYGLGTVLSSDGSGPRATVTIDFGSSGKVRLMLLGGVPMEKL
>Mature_763_residues
MNTSPFTPGSPDLIDGLNEQQRAAVEHIGSPLLIVAGAGSGKTAVLTRRIAYLMRYRGVHPQQILAITFTNKAAAEMRER
VSQLVGPVAERMWVATFHSVCVRILRQQAQLVEGLNTNFTIYDSDDSRRLLTMIAKDLELDIKKFSARTLLGAISNLKNE
LVTPQEALADAERTHNPYETVVARAFSEYQSRLRRANAVDFDDLIGETVRIFREHPPVAEYYRRRFRHVLIDEYQDTNHA
QYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFERDFSNARTILLEQNYRSTQTILSAANAVISQNENRR
PKNLWTALGEGEQIIGYVADNEHDEARFIASEIDNLVDHGMNYSDIAIMYRTNNSSRALEDVFMRTGVPYKVVGGTKFYE
RKEIRDIIAYLRVLENPDDTVNLRRIINTPKRGIGDRAQAFIALHSENNQISFGQALLDAGLGKIDLLGARGKNAVIKFN
ELFDALRTELPTMVNEVTGLPDIGQVISRILDITGYKAELEASNDPQDGARLDNLNELVSVAREFSSDAANRMVNEVPEG
EAQPGSLQAFLERVSLVADADQIPDSDNGVVTLMTLHTAKGLEFPIVFLTGWEDGQFPHLRSLGDAKELAEERRLAYVGI
TRARKRLYMTRAMLRSSWGNPVTNPPSRFLQEVPAELIDWRREEPQMSSAWAPRPTRSIPTKTRTNNKQLDLSVGDRVNH
DKYGLGTVLSSDGSGPRATVTIDFGSSGKVRLMLLGGVPMEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=736, Percent_Identity=40.4891304347826, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI48994965, Length=684, Percent_Identity=37.719298245614, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=384, Percent_Identity=26.3020833333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=760, Percent_Identity=27.7631578947368, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324477, Length=674, Percent_Identity=25.0741839762611, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85332; Mature: 85332

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSPFTPGSPDLIDGLNEQQRAAVEHIGSPLLIVAGAGSGKTAVLTRRIAYLMRYRGVH
CCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
PQQILAITFTNKAAAEMRERVSQLVGPVAERMWVATFHSVCVRILRQQAQLVEGLNTNFT
HHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IYDSDDSRRLLTMIAKDLELDIKKFSARTLLGAISNLKNELVTPQEALADAERTHNPYET
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
VVARAFSEYQSRLRRANAVDFDDLIGETVRIFREHPPVAEYYRRRFRHVLIDEYQDTNHA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
QYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFERDFSNARTILLEQNYR
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCEEEEEECCCH
STQTILSAANAVISQNENRRPKNLWTALGEGEQIIGYVADNEHDEARFIASEIDNLVDHG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
MNYSDIAIMYRTNNSSRALEDVFMRTGVPYKVVGGTKFYERKEIRDIIAYLRVLENPDDT
CCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCH
VNLRRIINTPKRGIGDRAQAFIALHSENNQISFGQALLDAGLGKIDLLGARGKNAVIKFN
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEHH
ELFDALRTELPTMVNEVTGLPDIGQVISRILDITGYKAELEASNDPQDGARLDNLNELVS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHCCHHHHHH
VAREFSSDAANRMVNEVPEGEAQPGSLQAFLERVSLVADADQIPDSDNGVVTLMTLHTAK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
GLEFPIVFLTGWEDGQFPHLRSLGDAKELAEERRLAYVGITRARKRLYMTRAMLRSSWGN
CCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
PVTNPPSRFLQEVPAELIDWRREEPQMSSAWAPRPTRSIPTKTRTNNKQLDLSVGDRVNH
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DKYGLGTVLSSDGSGPRATVTIDFGSSGKVRLMLLGGVPMEKL
CCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MNTSPFTPGSPDLIDGLNEQQRAAVEHIGSPLLIVAGAGSGKTAVLTRRIAYLMRYRGVH
CCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
PQQILAITFTNKAAAEMRERVSQLVGPVAERMWVATFHSVCVRILRQQAQLVEGLNTNFT
HHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IYDSDDSRRLLTMIAKDLELDIKKFSARTLLGAISNLKNELVTPQEALADAERTHNPYET
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
VVARAFSEYQSRLRRANAVDFDDLIGETVRIFREHPPVAEYYRRRFRHVLIDEYQDTNHA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCH
QYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFERDFSNARTILLEQNYR
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCEEEEEECCCH
STQTILSAANAVISQNENRRPKNLWTALGEGEQIIGYVADNEHDEARFIASEIDNLVDHG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
MNYSDIAIMYRTNNSSRALEDVFMRTGVPYKVVGGTKFYERKEIRDIIAYLRVLENPDDT
CCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCH
VNLRRIINTPKRGIGDRAQAFIALHSENNQISFGQALLDAGLGKIDLLGARGKNAVIKFN
HHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEHH
ELFDALRTELPTMVNEVTGLPDIGQVISRILDITGYKAELEASNDPQDGARLDNLNELVS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHCCHHHHHH
VAREFSSDAANRMVNEVPEGEAQPGSLQAFLERVSLVADADQIPDSDNGVVTLMTLHTAK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
GLEFPIVFLTGWEDGQFPHLRSLGDAKELAEERRLAYVGITRARKRLYMTRAMLRSSWGN
CCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
PVTNPPSRFLQEVPAELIDWRREEPQMSSAWAPRPTRSIPTKTRTNNKQLDLSVGDRVNH
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DKYGLGTVLSSDGSGPRATVTIDFGSSGKVRLMLLGGVPMEKL
CCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]