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The map label for this gene is pdxR [H]

Identifier: 145294950

GI number: 145294950

Start: 989875

End: 991236

Strand: Reverse

Name: pdxR [H]

Synonym: cgR_0896

Alternate gene names: 145294950

Gene position: 991236-989875 (Counterclockwise)

Preceding gene: 145294953

Following gene: 145294948

Centisome position: 29.91

GC content: 64.17

Gene sequence:

>1362_bases
ATGCTTGCCGACCTTCCCATCGCCTTAAACCCACACGAACCAACATCCATCCCCACGCAGCTCACAGAACAGATCCGCCG
TCTCGTGGCGAGGGGAATTCTCACCCCAGGAGACCCGCTTCCCAGCAGTCGCTCGCTATCCACCCAATTGGGGGTATCCC
GCGGCAGTGTGGTGACCGCTTATGACCAATTGGCCGGTGAAGGCTACCTCAGCACCGCCCGCGGTTCCGGTACAACGATC
AACCCAGATCTGCATTTGTTGAAGCCGGTGGAAATTGAGAAGAAGGAGACGTCGAGAAGCGTCCCGCCCCCGCTGCTCAA
CCTGAGCCCCGGCGTGCCCGATACCGCGACGCTCGCCGATTCCGCATGGCGCGCTGCGTGGCGCGAAGCCTGCGCCAATC
CACCCACGAACTCCCCTGAGCAGGGACTTTTGAGGCTGCGGATCGAGATCGCCGACCACCTGCGCCAGATGCGTGGCCTC
ATGGTCGAGCCGGAGCAGATCATCGTCACCGCCGGCGCACGCGAGGGGCTGAGTCTGCTGCTGCGCACCATGGATGCGCC
TGCCCGCATCGGCGTCGAATCGCCCGGCTACCCCAGCCTGCGCCGCATCCCGCAGGTGCTTGGCCATGAGACGATCGATG
TGCCGACCGACGAATCCGGCCTCGTACCCCGCGCGCTGCCCCACGACCTCAACGCGCTACTGGTAACCCCTAGCCATCAA
TATCCCTACGGCGGCTCGCTGCCCGCCGATCGCCGCACCGCGCTAGTCGCGTGGGCTGAGGCAAACGATGCGTTGCTTAT
TGAAGACGACTTCGATTCTGAGCTGCGCTACGTCGGTATGCCGCTTCCGCCGCTGCGTGCGCTGGCGCCCGATCGCACGA
TTCTGCTCGGCACGTTTTCCTCCGTGATCACACCACAAGTCGCCTGCGGATACCTCATCGCGCCGACGCCCCAGGCGCGC
GTGCTCGCCACGCTTCGAGGGATTCTCGGCCAGCCAGTCGGCGCCATCACCCAACACGCGCTCGCTTCCTACCTCGCATC
GGGTGCTTTACGACGCCGCACCCAACGTTTGCGGCGCCTTTACCGACACCGCCGCTCCATCGTCCAAGACACCCTCGGTG
ACCTCCCGAATACACAGCTTCGCCCCATCAACGGCGGTCTCCACGCTGTACTTCTGTGCGACAAACCCCAAGAGCTCGTC
GTCTCTACACTGGCCTCCCGTGGCCTCAACGTCACCGCGCTCTCCCACTACTGGGGCGGCACCGGCGCAGACAACGGCAT
CGTTTTCGGCTTCGGCTCCCACGACGAAGACACCCTTAGATGGGTGCTTGCTGAGATCAGCGATGCGGTGTCTCTGGGCT
AA

Upstream 100 bases:

>100_bases
GTTTCGGTCATGACAGATGATCCAACGCCACAAAGTGGACTAGCGGTAGATCCACTTTCAGCCACTTGCATTAGACCACT
TTTTTGAGGACGATGAAGCC

Downstream 100 bases:

>100_bases
AGAAAAAACAGCCCGAGAGGAACGATCCTCTCGGGCTGTTTACTCAAACGCTACTGTGCGGACTGGTAGCTATCGACGGC
ACGATCAAGCGCATCGAGTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACANPPTNSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQELV
VSTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG

Sequences:

>Translated_453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACANPPTNSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQELV
VSTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
>Mature_453_residues
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTAYDQLAGEGYLSTARGSGTTI
NPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLADSAWRAAWREACANPPTNSPEQGLLRLRIEIADHLRQMRGL
MVEPEQIIVTAGAREGLSLLLRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFSSVITPQVACGYLIAPTPQAR
VLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRLYRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQELV
VSTLASRGLNVTALSHYWGGTGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG

Specific function: May have a regulatory function in pyridoxine biosynthesis. Is said to also have an aminotransferase activity in valine biosynthesis as a double inactivation of ilvE and pdxR results in an auxotrophic requirement for valine [H]

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790797, Length=459, Percent_Identity=26.5795206971678, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787710, Length=380, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 48822; Mature: 48822

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEH
YDQLAGEGYLSTARGSGTTINPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLAD
HHHHCCCCCEEECCCCCCEECCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHH
SAWRAAWREACANPPTNSPEQGLLRLRIEIADHLRQMRGLMVEPEQIIVTAGAREGLSLL
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
LRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFS
CCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEECCCCCCHHHCCCCCEEEEECHH
SVITPQVACGYLIAPTPQARVLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRL
HHHCHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQELVVSTLASRGLNVTALSHYWGG
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCCC
TGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLADLPIALNPHEPTSIPTQLTEQIRRLVARGILTPGDPLPSSRSLSTQLGVSRGSVVTA
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEH
YDQLAGEGYLSTARGSGTTINPDLHLLKPVEIEKKETSRSVPPPLLNLSPGVPDTATLAD
HHHHCCCCCEEECCCCCCEECCCCEEECCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHH
SAWRAAWREACANPPTNSPEQGLLRLRIEIADHLRQMRGLMVEPEQIIVTAGAREGLSLL
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHH
LRTMDAPARIGVESPGYPSLRRIPQVLGHETIDVPTDESGLVPRALPHDLNALLVTPSHQ
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
YPYGGSLPADRRTALVAWAEANDALLIEDDFDSELRYVGMPLPPLRALAPDRTILLGTFS
CCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCEEECCCCCCHHHCCCCCEEEEECHH
SVITPQVACGYLIAPTPQARVLATLRGILGQPVGAITQHALASYLASGALRRRTQRLRRL
HHHCHHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRHRRSIVQDTLGDLPNTQLRPINGGLHAVLLCDKPQELVVSTLASRGLNVTALSHYWGG
HHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCCC
TGADNGIVFGFGSHDEDTLRWVLAEISDAVSLG
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948641; 12948626 [H]