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The map label for this gene is thtR [H]

Identifier: 145294874

GI number: 145294874

Start: 909128

End: 910018

Strand: Reverse

Name: thtR [H]

Synonym: cgR_0821

Alternate gene names: 145294874

Gene position: 910018-909128 (Counterclockwise)

Preceding gene: 145294876

Following gene: 145294873

Centisome position: 27.46

GC content: 58.36

Gene sequence:

>891_bases
ATGGCAGCACCGTTCGACCCATTCCCAGCTTTTGAGGAATACGCACACCCTGAAAGGATCGTGTCTGCATCCTGGCTTTC
CGCCCGCCTCGGCTCCCCTGGTCTGAAGGTTGTGGAGTCCAATGAAGATTCCCTTCTTTACGATATCGGACACCTCCCCG
GCGCTGTGCGCATTGACTGGGCAAAGGACCTCAACGATCCCCTCACCCGCGATTTCATTGACGGTGAAGCTTTCGCTGAG
CTGATGAACCGCAAGGGCATCGCTCGCGATGACACCGTTGTTGTCTACGGTGACAAGTCCAACTGGTGGGCTGCGTTCAC
CCTGTGGGTCTTCGAACTGTTCGGCCACTCCGATGTCCGCCTGCTCAACGGCGGCCGTGACGCGTGGATGGCTGAAGAGC
GCGACACCTCCTACGTGGTTCCGGAGTACCCCTCCGCCAACTACCCCGTCGTCGAGCGTGTCGACGAAAACCAGCGCGCG
TTCGTGGCTGAGGTGCTCGGTTCGCTCACGCAATCCGGTGGCATGACGCTTGTCGACGTCAGGACCCCTTCGGAGTTCTC
CGGATTGGATGAGCACGGCAACCCAACCTCAAACACCGGCGTGCTTCGTGGTGGACACATCCCAGGCGCGATCAACCTGG
ATTGGTCGGACGCTGTTCTTCCCAACGGAAACTTCCGCACCCGTGCAGAGTTGGACAAGCTCTACGCTGATCTCAACCCA
GCTGACGATACCGTTGTCTACTGCCAGGTTGGCGACCGCGCGGCCCACACCTGGTTCGTGCTGAAGTATCTGCTCGGTTT
CAACAACGTCCGAAACTATGACGGATCGTGGGCAGAATGGGGCAATATGGTTCGCATGCCGATCGAAACTGGCGAAAACA
CCAAAAAATAA

Upstream 100 bases:

>100_bases
CTAGTAATTAAGGACACAAACACCTTTAACCAATATGCTGGGATACAGTATTTAATTTTCACCCATTTATGCACTCGTTT
ATGGAAGGCAGCGACCCCAC

Downstream 100 bases:

>100_bases
CGTTTTGGTGTCATAGAATAGGCGTATCCCCTTTTTGCCTAATCAAAAGCCATATTTCCTGCGTAAAACAGAGGAATTCC
CCCAGAATATGAGTAAATCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAE
LMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRA
FVAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKK

Sequences:

>Translated_296_residues
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAE
LMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRA
FVAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKK
>Mature_295_residues
AAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAEL
MNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAF
VAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNPA
DDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKK

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=290, Percent_Identity=31.0344827586207, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=28.1021897810219, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=28.1021897810219, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=28.1021897810219, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082121, Length=282, Percent_Identity=28.0141843971631, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081967, Length=300, Percent_Identity=28, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17561888, Length=277, Percent_Identity=25.9927797833935, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=226, Percent_Identity=29.2035398230088, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997283, Length=235, Percent_Identity=26.8085106382979, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=240, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33022; Mature: 32891

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDW
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDPLTRDFIDGEAFAELMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVR
HHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
LLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAFVAEVLGSLTQSGGMTLVDV
EEECCCCCCEECCCCCCEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
RTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
CCCCHHCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKK
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
AAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDW
CCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDPLTRDFIDGEAFAELMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVR
HHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
LLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAFVAEVLGSLTQSGGMTLVDV
EEECCCCCCEECCCCCCEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
RTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
CCCCHHCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKK
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626; 8772169 [H]