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The map label for this gene is dacB [H]

Identifier: 145294853

GI number: 145294853

Start: 883640

End: 884950

Strand: Reverse

Name: dacB [H]

Synonym: cgR_0800

Alternate gene names: 145294853

Gene position: 884950-883640 (Counterclockwise)

Preceding gene: 145294854

Following gene: 145294849

Centisome position: 26.7

GC content: 57.28

Gene sequence:

>1311_bases
ATGAGGAGATCACTTCGACACGGATTCACAGCACTTCTGACAACGTGGGCGCTACTTTTACCCACTGTTGCGGTAGCACA
GGAACCGATCGACAACGTCCCCGAAGTGACAGCCGACAACGCAGAAACTCTCGAAGACATCATCGACGACGCGCTTACCC
CCACCACCCGGTCGGAAGCTCCCAACACTGATCTGTGCCCCTACAAGGAATCCCCTTCCCCGGCGCGCAGCACATCAGAG
GTAGTCGCGCCGGGTGCACAAACGCCCGCGCCGGTTCCGGTTCCATCTGTTCCCGCAGGTGGTGAGAAATTGGCGACATG
CGGTGTGATCACTCCCAATGGCTTTGAGCTTCCTGAAGATTCTGATGAAACTATCACTGCGTCGGCGTGGATGGTGTTTG
ATATTGATTCCGGTGAGATCATCGCAGCAAAGGATCCCCACGGAAGGTATCGCCCCGCATCGATCATCAAGGTGTTACTC
AGCTTGGTTGCCATCGATGAGCTGAACCCCACCACCGTGGTCACGGGCACGATGGAAGCCGCCAACATTGAGGGCTCCCG
CGTGGGTGTCGGCGAGGGCGGCCAGTATACCGTTGATCAGCTGCTGCACGGTCTTCTTTTAGCCAGCGGTAACGATGCGG
CGTATCTGTTGGCTCAGGAACTTGGTGGGGATCAAGCAACCCTGGAGAAAGTAAACGCGCTGGCCAAGGAGTTGGGCACT
CAAGACACCTTCGTTGCCACTTATTCCGGTTTGGATGCGCCGGGAATGTCGACCTCCGCATACGACATGTCATTGATTTA
TCAGCATGCGTGGCAGAACCCGGTTTTCGAGTCGATTATCTCCACCGATCACATTGATTTCCCTGGTTGGGGCGATAATG
AGGGTTTCCAAGTCTGGAACGATAACGCCTTGTTCATGAACGATCCTGATGGCATCGGCGGCAAGACCGGCTACACCGAC
GACGCGAACCACACCTTTGTCGGCGGTCTCGATCGGGGTGGTCGCCGCCTCGCCGCCGTACTCTTGGATTCCACCGTCAG
CGACATTCGTCCGTGGGAACAAGCGCGTTTGCTTATCGACGCCTCCCTCCCCATCACGCCGGGGTCCGGCGTGGGCCAGC
TAGGCTCCGGCAGCGCGAACGATGTGGCACCGGCGACCCCAGAATTACCAGAACCCACCGACAACCTGACTTCAGGTGAG
GGTGGGTCTCAGAACACGCTGCTTAAGCTCATGGTGCCCATCGGAATCATCGTGCTGTTGCTAATCGCCGCACTAGCGTG
GACATTCAGATCTCCCAAGAAAAAGAACTAG

Upstream 100 bases:

>100_bases
AATAGCCGCCCAGCCGGCCACGACATCACAGGCACACAGACACGCCTAAACACCACCGGTCCCTCGTCGAGCACGCAAGA
AAAGGTAATATGTCCAACCC

Downstream 100 bases:

>100_bases
GTGTTCTTCTTCACGACCTCTGCGTTGGCCAAGCGGTCGTGCATACCGCGCATCTGATTGCTTTCATTAATGGAAGAGCC
ATAAACGATGGCCATCACAA

Product: hypothetical protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRRSLRHGFTALLTTWALLLPTVAVAQEPIDNVPEVTADNAETLEDIIDDALTPTTRSEAPNTDLCPYKESPSPARSTSE
VVAPGAQTPAPVPVPSVPAGGEKLATCGVITPNGFELPEDSDETITASAWMVFDIDSGEIIAAKDPHGRYRPASIIKVLL
SLVAIDELNPTTVVTGTMEAANIEGSRVGVGEGGQYTVDQLLHGLLLASGNDAAYLLAQELGGDQATLEKVNALAKELGT
QDTFVATYSGLDAPGMSTSAYDMSLIYQHAWQNPVFESIISTDHIDFPGWGDNEGFQVWNDNALFMNDPDGIGGKTGYTD
DANHTFVGGLDRGGRRLAAVLLDSTVSDIRPWEQARLLIDASLPITPGSGVGQLGSGSANDVAPATPELPEPTDNLTSGE
GGSQNTLLKLMVPIGIIVLLLIAALAWTFRSPKKKN

Sequences:

>Translated_436_residues
MRRSLRHGFTALLTTWALLLPTVAVAQEPIDNVPEVTADNAETLEDIIDDALTPTTRSEAPNTDLCPYKESPSPARSTSE
VVAPGAQTPAPVPVPSVPAGGEKLATCGVITPNGFELPEDSDETITASAWMVFDIDSGEIIAAKDPHGRYRPASIIKVLL
SLVAIDELNPTTVVTGTMEAANIEGSRVGVGEGGQYTVDQLLHGLLLASGNDAAYLLAQELGGDQATLEKVNALAKELGT
QDTFVATYSGLDAPGMSTSAYDMSLIYQHAWQNPVFESIISTDHIDFPGWGDNEGFQVWNDNALFMNDPDGIGGKTGYTD
DANHTFVGGLDRGGRRLAAVLLDSTVSDIRPWEQARLLIDASLPITPGSGVGQLGSGSANDVAPATPELPEPTDNLTSGE
GGSQNTLLKLMVPIGIIVLLLIAALAWTFRSPKKKN
>Mature_436_residues
MRRSLRHGFTALLTTWALLLPTVAVAQEPIDNVPEVTADNAETLEDIIDDALTPTTRSEAPNTDLCPYKESPSPARSTSE
VVAPGAQTPAPVPVPSVPAGGEKLATCGVITPNGFELPEDSDETITASAWMVFDIDSGEIIAAKDPHGRYRPASIIKVLL
SLVAIDELNPTTVVTGTMEAANIEGSRVGVGEGGQYTVDQLLHGLLLASGNDAAYLLAQELGGDQATLEKVNALAKELGT
QDTFVATYSGLDAPGMSTSAYDMSLIYQHAWQNPVFESIISTDHIDFPGWGDNEGFQVWNDNALFMNDPDGIGGKTGYTD
DANHTFVGGLDRGGRRLAAVLLDSTVSDIRPWEQARLLIDASLPITPGSGVGQLGSGSANDVAPATPELPEPTDNLTSGE
GGSQNTLLKLMVPIGIIVLLLIAALAWTFRSPKKKN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=248, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786851, Length=248, Percent_Identity=31.4516129032258, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787062, Length=264, Percent_Identity=29.9242424242424, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45981; Mature: 45981

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSLRHGFTALLTTWALLLPTVAVAQEPIDNVPEVTADNAETLEDIIDDALTPTTRSEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PNTDLCPYKESPSPARSTSEVVAPGAQTPAPVPVPSVPAGGEKLATCGVITPNGFELPED
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
SDETITASAWMVFDIDSGEIIAAKDPHGRYRPASIIKVLLSLVAIDELNPTTVVTGTMEA
CCCEEEEEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHH
ANIEGSRVGVGEGGQYTVDQLLHGLLLASGNDAAYLLAQELGGDQATLEKVNALAKELGT
CCCCCCEEECCCCCCEEHHHHHHHHEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC
QDTFVATYSGLDAPGMSTSAYDMSLIYQHAWQNPVFESIISTDHIDFPGWGDNEGFQVWN
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEC
DNALFMNDPDGIGGKTGYTDDANHTFVGGLDRGGRRLAAVLLDSTVSDIRPWEQARLLID
CCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
ASLPITPGSGVGQLGSGSANDVAPATPELPEPTDNLTSGEGGSQNTLLKLMVPIGIIVLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIAALAWTFRSPKKKN
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRSLRHGFTALLTTWALLLPTVAVAQEPIDNVPEVTADNAETLEDIIDDALTPTTRSEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
PNTDLCPYKESPSPARSTSEVVAPGAQTPAPVPVPSVPAGGEKLATCGVITPNGFELPED
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
SDETITASAWMVFDIDSGEIIAAKDPHGRYRPASIIKVLLSLVAIDELNPTTVVTGTMEA
CCCEEEEEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHH
ANIEGSRVGVGEGGQYTVDQLLHGLLLASGNDAAYLLAQELGGDQATLEKVNALAKELGT
CCCCCCEEECCCCCCEEHHHHHHHHEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC
QDTFVATYSGLDAPGMSTSAYDMSLIYQHAWQNPVFESIISTDHIDFPGWGDNEGFQVWN
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEC
DNALFMNDPDGIGGKTGYTDDANHTFVGGLDRGGRRLAAVLLDSTVSDIRPWEQARLLID
CCEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
ASLPITPGSGVGQLGSGSANDVAPATPELPEPTDNLTSGEGGSQNTLLKLMVPIGIIVLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LIAALAWTFRSPKKKN
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]